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1.
《Comparative biochemistry and physiology. A, Comparative physiology》1987,86(1):141-146
- 1.1. Morphological similarities and differences for the urticating apparatus of three Lepidoptera were studied using a scanning electron microscope.
- 2.2. Complementary anatomical studies of the urticant apparatus were undertaken to explain the morphological results.
- 3.3. Biochemical identity of a thaumetopoein-like protein (an urticating protein) was demonstrated for Thaumatopoea urticating hairs but not for Hylesia moth spicules.
- 4.4. Urticating mechanisms appear to be different across species of Lepidoptera.
2.
《Molecular & cellular proteomics : MCP》2019,18(10):2003-2017
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- •Identification of the first evolutionary divergent sirtuin ScCobB2 in bacteria.
- •Implementing a global quantitative succinylome between ΔScCobB2 and WT cells.
- •ScCobB2 regulates S. coelicolor protein biosynthesis and carbon metabolism pathways.
- •The divergent sirtuin enzymes are prevalent in other groups of Actinobacteria.
3.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1992,101(4):929-932
- 1.1. In Allolobophora caliginosa, a Cd-binding protein distinct in charge from Cd-BP 14, a Cd-binding protein previously isolated from the same oligochaete species [Nejmeddine et al. (1992) Comp. Biochem. Physiol.101C, 601–605], has been purified by a three-step chromatographic procedure including gel permeation and cation-exchange chromatography.
- 2.2. This Cd-binding protein exists in a monomeric form with a molecular weight of 14 kDa and does not contain carbohydrate.
- 3.3. The purified protein significantly absorbed at 280 nm and its amino acid composition revealed the presence of a high level of aromatic amino acids and a lack of cysteine, indicating that the molecule is distinct from metallothioneins.
- 4.4. By contrast, except for its chromatographic behavior on an ion-exchange chromatography column, the metalloprotein was found to be similar to Cd-BP 14. We thus conclude that it represents a charge-variant of Cd-BP 14.
4.
《Molecular & cellular proteomics : MCP》2018,17(12):2412-2433
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- •Proteogenomics and secretome comparison of human and zoonotic Staphylococcus aureus lineages.
- •869 secreted proteins identified in eight S. aureus isolates of CC8, CC22 and CC398.
- •CC398 lower secretion of surface proteins and higher secretion of hemolysins and exoenzymes.
- •Regulatory differences in the secretomes could be linked to lower SigB activity in CC398.
5.
《The International journal of biochemistry》1984,16(2):129-145
- 1.1. The chemical identification of spatial arrangements of the subunits in oligomeric proteins is exclusively achieved by the analysis of the reaction products of the protein and bifunctional reagents.
- 2.2. Since the pioneer work of Hartman and Wold (Biochemistry6, 2439–2448, 1967) the bifunctional reagent such as bis-imido-esters was first introduced into protein chemistry.
- 3.3. We have listed the non-cleavable and cleavable bis-imido-esters, the N-hydroxy-succinimido-csters and the aryl azides which once photolyzed, become the highly reactive nitrene intermediates.
- 4.4. Different reagents classified as homo- and hetero-bifunctional reagents are also listed.
- 5.5. The advantages and limits of each group as well as their chemical properties are advanced and extensively discussed.
6.
《Molecular & cellular proteomics : MCP》2019,18(10):2078-2088
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- •Proteomic landscapes of Drosophila somatic and reproductive tissues during aging.
- •Pulsed metabolic labeling determines a decline in protein synthesis with age.
- •Drosophila model of human Parkinson's disease signifies an early-onset decline in protein synthesis.
- •Collapse of proteostasis and mitochondria are early signals for normal aging.
7.
《Molecular & cellular proteomics : MCP》2019,18(8):1572-1587
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- •Protein acetylation at Lys residues and the N terminus occurs widely in Sulfolobus.
- •SisPat preferentially acetylates a group of acyl-CoA synthetases.
- •SisArd1 acetylates the majority of the acetylated N termini identified in the cell.
- •SisArd1, but not SisPat, is required for the optimal growth of the organism.
8.
9.
《Molecular & cellular proteomics : MCP》2019,18(7):1437-1453
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- •mRNA-seq, miRNA-seq, proteomes of P. fulvidraco, P. vachelli, hybrid Huangyou-1.
- •Predicted miRNA-mRNA-protein pairs were found and validated by qRT-PCR and PRM.
- •Immune, metabolism, digestion, absorption, proliferation, development generate heterosis.
- •High parental gene/protein with low parental miRNAs inherit from the mother or father.
10.
《Molecular & cellular proteomics : MCP》2019,18(8):1669-1682
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- •Spatiotemporal microproteomics analysis of TBI.
- •Injury site microproteomics reveal distinct phases in 10-day frame post TBI.
- •Uninjured proteomic profile is restored in TBI at 10 days post injury.
- •Substantia nigra protein post 3 days suggest link to Parkinson's disease.
11.
《Comparative biochemistry and physiology. A, Comparative physiology》1993,104(3):571-579
- 1.1. Mineral balance was studied in meadow voles (Microtus pennsylvanicus) maintained in the laboratory.
- 2.2. Urine and fecal Na+ contents of voles on low-Na+ diets were comparable to those reported for other herbivore species, but urine and fecal K levels were higher.
- 3.3. Voles approached Na+ balance (input = output) on diets with Na+ content as low as 56 ppm.
- 4.4. There was not a clearcut hypertrophy of the adrenal-gland zona glomerulosa in voles maintained on low-Na+ diets.
- 5.5. Plasma K content and bone water content were higher in voles maintained on high-Na + vegetation diets, suggesting expansion of extracellular fluid volume.
12.
《Molecular & cellular proteomics : MCP》2019,18(11):2191-2206
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- •Quantitative high-throughput glycoanalytical technology as a diagnostic tool for ovarian cancer detection.
- •Multiplexed approach harnessing N-glycan data for six glycoproteins from a single biological sample.
- •Detailed characterization of human serum N-glycans from antibodies IgG, IgM and IgA and acute phase proteins transferrin, haptoglobin and alpha-1-antitrypsin.
- •Structural differences in antibody and acute phase protein glycosylation for mechanistic insights.
13.
《Molecular & cellular proteomics : MCP》2019,18(11):2138-2148
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- •Highly glycosylated matrisome proteins and their binding partners comprise extracellular networks that mediate tissue-specific cellular microenvironments.
- •Elucidation of roles of matrisome molecules in disease mechanisms requires detailed mapping of matrisome glycosylation and other post-translational modifications.
- •We review tissue workup methods for matrisome proteomics, glycomics and glycoproteomics.
- •The combination of proteomics, glycomics and glycoproteomics profiles matrisome protein modifications distinct from those studied by immunohistochemistry.
14.
《Molecular & cellular proteomics : MCP》2019,18(12):2516-2523
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- •Open source software for comprehensive HDX-MS data analysis.
- •Automatic back-exchange correction options.
- •Rigorous statistical analysis of the significance of uptake differences.
- •High quality visualization tools.
15.
《Molecular & cellular proteomics : MCP》2019,18(1):115-126
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- •Application of Sortase A to label protein N-termini across the whole proteome.
- •Novel gel, proteomic and ELISA-based methods to determine N-myristoylation of proteins in cells, without metabolic labelling.
- •Side by side mass spectrometric quantification of changes in protein N-myristoylation by two complementary methods.
- •Improved Biotin-Neutravidin affinity enrichment protocol.
16.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1993,104(1):115-124
- 1.1. Nematocyst structural proteins (NSP) from the sea anemones Aiptasia pallida and Metridium senile and the siphonophore Physalia physalis are primarily low molecular weight collagens linked by disulfide bonds.
- 2.2. NSP patterns resolved by SDS-PAGE revealed a common, major collagen species (40 kDa) in each nematocyst type, together with other collagens and non-thiol-containing proteins.
- 3.3. For each cnidarian, NSP glycosylation profiles were significantly different.
- 4.4. Monoclonal antibodies against Aiptasia NSP demonstrated a differential distribution between capsule wall and thread.
- 5.5. NSP differences would account for the diversity of morphologic and functional types.
17.
《Molecular & cellular proteomics : MCP》2019,18(5):1027-1035
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- •Insoluble protein aggregates preferentially precipitate on microparticles.
- •The protein aggregation capture (PAC) occurs irrespective of microparticle surface chemistry.
- •This process can be exploited for multi-purpose proteomics sample preparation.
- •Facilitates potential for low cost, efficient and high-sensitivity proteomics workflows.
18.
《Molecular & cellular proteomics : MCP》2019,18(10):2029-2043
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- •LFQ-based protein patterns define glioma subgroups that correlate with genomic alterations.
- •Proteomic analysis resolves distinct pathway-level differences among glioma subtypes.
- •Distinct IDH subtype-specific tumor patterns are maintained in glioma stem cell cultures.
19.
《Comparative biochemistry and physiology. C: Comparative pharmacology》1988,89(2):327-331
- 1.1. A potentiometric method for the assay of cholinesterase has been proposed and compared with a colorimetric assay.
- 2.2. Main kinetic parameters of cholinesterase from Hypostomus punctatus brain were determined indicating that true acetylcholinesterase is by far the predominant enzyme in the brain of this fish.
- 3.3. We have compared our data with published results described from other fish species.
- 4.4. The enzyme inhibition achieved after 3 hr incubation of brain homogenates with ethyl-parathion have indicated that this enzyme shows a characteristic organophosphorous sensitive behavior.
20.
Domain-specific Quantification of Prion Protein in Cerebrospinal Fluid by Targeted Mass Spectrometry
《Molecular & cellular proteomics : MCP》2019,18(12):2388-2400
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- •Targeted mass spectrometry assay to quantify prion protein (PrP) in spinal fluid.
- •Precise measurement of PrP peptide concentration across protein domains.
- •Peptides are uniformly decreased in symptomatic prion disease patients.
- •Assay applicable to humans and preclinical species for drug development.