共查询到20条相似文献,搜索用时 601 毫秒
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Zulak KG Cornish A Daskalchuk TE Deyholos MK Goodenowe DB Gordon PM Klassen D Pelcher LE Sensen CW Facchini PJ 《Planta》2007,225(5):1085-1106
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Integrated analysis of metabolite and transcript levels reveals the metabolic shifts that underlie tomato fruit development and highlight regulatory aspects of metabolic network behavior
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Carrari F Baxter C Usadel B Urbanczyk-Wochniak E Zanor MI Nunes-Nesi A Nikiforova V Centero D Ratzka A Pauly M Sweetlove LJ Fernie AR 《Plant physiology》2006,142(4):1380-1396
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Lucila Peluffo Verónica Lia Carolina Troglia Paniego Norma Alberto Escande Anna Lytovchenko Ruth Heinz Fernando Carrari 《Phytochemistry》2010,71(1):70-80
We report a comprehensive primary metabolite profiling of sunflower (Helianthus annuus) genotypes displaying contrasting behavior to Sclerotinia sclerotiorum infection. Applying a GC-MS-based metabolite profiling approach, we were able to identify differential patterns involving a total of 63 metabolites including major and minor sugars and sugar alcohols, organic acids, amino acids, fatty acids and few soluble secondary metabolites in the sunflower capitulum, the main target organ of pathogen attack. Metabolic changes and disease incidence of the two contrasting genotypes were determined throughout the main infection period (R5.2-R6). Both point-by-point and non-parametric statistical analyses showed metabolic differences between genotypes as well as interaction effects between genotype and time after inoculation. Network correlation analyses suggested that these metabolic changes were synchronized in a time-dependent manner in response to the pathogen. Concerted differential metabolic changes were detected to a higher extent in the susceptible, rather than the resistant genotype, thereby allowing differentiation of modules composed by intermediates of the same pathway which are highly interconnected in the susceptible line but not in the resistant one. Evaluation of these data also demonstrated a genotype specific regulation of distinct metabolic pathways, suggesting the importance of detection of metabolic patterns rather than specific metabolite changes when looking for metabolic markers differentially responding to pathogen infection. In summary, the GC-MS strategy developed in this study was suitable for detection of differences in carbon primary metabolism in sunflower capitulum, a tissue which is the main entry point for this and other pathogens which cause great detrimental impact on crop yield. 相似文献
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In this work we show how three types of cucumber in vitro cultures – leaf callus culture, cytokinin dependent cell suspension and liquid culture of meristematic clumps – influence the metabolite profiles of plants in the first generative progeny. Based on this study we conclude that there exists a specific and inheritable metabolic fingerprint reflecting the history of previous generations, probably related to specific stress factors accompanying the passage through different types of culture. The leaf callus culture generated the highest heritable differences in metabolite content and was the most distinctly separated cluster in PCA analysis. The smallest number of variable metabolites characterizes the plants regenerated from cytokinin dependent cell suspension whereas the liquid culture of meristematic clumps induced slightly more changes. Changes induced by these two culture types were not as pronounced as in the case of leaf callus culture. However the plants after these types of culture were well separated from the control on PCA diagram. The highest changes were over 2-fold increases in cystin and galactose-6-P and over 2-fold decreases in aspartate, myo-inositol, hydroxylamine, phosphate and putrescine. These changes concerned the plants, which were one generation after the leaf callus culture. The possible nature of observed heritable changes is discussed. 相似文献
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Integrated analysis of rice transcriptomic and metabolomic responses to elevated night temperatures identifies sensitivity‐ and tolerance‐related profiles
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Ulrike Glaubitz Xia Li Sandra Schaedel Alexander Erban Ronan Sulpice Joachim Kopka Dirk K. Hincha Ellen Zuther 《Plant, cell & environment》2017,40(1):121-137
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《Cryobiology》2017
Comparative quantitative metabolite profiling can be used for better understanding of cell functions and dysfunctions in particular circumstances such as sperm banking which is an important approach for cryopreservation of endangered species. Cryopreservation techniques have some deleterious effects on spermatozoa which put the obtained results in controversy. Therefore, in the present study, quantitative 1H NMR (Nuclear Magnetic Resonance) based metabolite profiling was conducted to evaluate metabolite changes related to energetics and some other detected metabolites in vitrified semen of critically endangered wild Acipenser persicus. The semen was diluted with extenders containing 0, 5, 10, and 15 μM of fish antifreeze protein (AFP) type III as a cryoprotectant. Semen-extenders were vitrified and stored for two days. Based on post-thaw motility duration and motility percentage assessments, two treatments with 10 μM and 0 μM of AFP had the highest and the lowest motility percentages respectively and they were objected to 1H NMR spectroscopy investigations in order to reveal the extremes of the metabolites dynamic range. Univariate (ANOVA) and multivariate (PCA) analysis of the resulting metabolic profiles indicated significant changes (P > 0.05) in metabolites. The level of some metabolites including acetate, adenine, creatine, creatine phosphate, lactate, betaine, sarcosine, β-alanine and trimethylamine N-oxide significantly decreased in vitrified semen while some others such as creatinine, guanidinoacetate, N, N-dimethylglycine, and glycine significantly increased. There were also significant differences between vitrified treatments in levels of creatine, creatine phosphate, creatinine, glucose, guanidinoacetate, lactate, N, N-dimethylglycine, and glycine, suggesting how fish AFP type III can be effective as a cryoprotectant. 相似文献
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Identification of primary and secondary metabolites with phosphorus status‐dependent abundance in Arabidopsis,and of the transcription factor PHR1 as a major regulator of metabolic changes during phosphorus limitation
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BIKRAM‐DATT PANT POOJA PANT ALEXANDER ERBAN DAVID HUHMAN JOACHIM KOPKA WOLF‐RÜDIGER SCHEIBLE 《Plant, cell & environment》2015,38(1):172-187