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1.
B. M. Kapoor 《Genetica》1966,37(1):557-568
A study of the cytology of endosperm ofPisum sativum, pea, fixed at different stages of development reveals that it remains free nuclear throughout its entire life. The nuclei are extremely polymorphic and differ in size from each other. The nuclei increase proportionately in size with the advancement in endosperm age.The haploid chromosome number of the taxon was verified asn=7. The endosperm nuclei were normally triploid with 3n=21 chromosomes, but higher polyploidy (6n and 12n) and aneuploidy were also recorded in small proportions. Nuclear fusions and aberrations such as irregular separation of chromosomes, sticky bridges and laggards are believed to be responsible for the origin of polyploid nuclei.Accumulation of mitotic aberrations including bridges and laggards are considered to result in reduced divisional activity thereby leading to endosperm breakdown with the consequent low seed set in some cases.  相似文献   

2.
The development of the endosperm in Gleditsia is marked by the growth of the average nucleus from 141 μ3 on June 12 to 978 μ3 on July 14. At the time of formation of a cellular endosperm the average nuclear volume commences dropping and by July 20 reaches an average of 224 μ3 throughout the endosperm. In Podophyllum at the time of cell formation the average nuclear volume begins to drop from a value of about 2850 μ3 and falls to a value of from 500 μ3 to 770 μ3, the latter estimates depending upon the mode of fixation of the material. The volume decrease in the Podophyllum endosperm nucleus is accompanied by a fall in mean relative DNA content of the average nucleus, as determined by microspectrophotometry with the two wavelength method. The possibility of degeneration of some of the higher “ploid” types is considered, as well as depolyploidization.  相似文献   

3.
Most Liliaceae plants have the tetrasporic Fritillaria-type embryo sac and normally form diploid embryos and pentaploid endosperms derived from a 4:1 maternal-to-paternal genome ratio (4m:1p) after double fertilization. Here we characterize embryo sac and endosperm formation in Tulipa spp. of Liliaceae. Chromosome analysis using seeds derived from 2x × 2x crosses of Tulipa gesneriana (2n = 2x = 24) identified diploid chromosome number in the endosperm. Similarly, flow cytometric analysis confirmed diploid endosperm formation in T. gesneriana, T. fosteriana (2n = 2x = 24) and T. greigii (2n = 2x = 24). To further study the possible mechanism of diploid endosperm formation, we made interploidy crosses of triploid (2n = 3x = 36) × diploid in which aneuploid seeds with various chromosome numbers (2n = 25–36) were produced. Again, flow cytometric analysis confirmed the same ploidy level in both embryos and endosperms at all aneuploidy levels, suggesting that only a single haploid polar nucleus contributes to endosperm formation at fertilization. Histological observation further confirmed the physical separation of two polar nuclei by a large vacuole in the Fritillaria-type embryo sac of T. gesneriana that appeared to prevent the fusion of the two polar nuclei that originated at the micropylar and chalazal ends before fertilization. Taken together, these results indicate that diploid endosperms (1m:1p) are normally formed in Tulipa spp. by fusion of the micropylar polar nucleus (n) and a spermatid (n) but not by normal triple fusion. We also show that tulip endosperm partially overcomes the triploid block mechanism that occurs in interploidy crosses. Based on these observations, the possible role of triple nuclear fusion in double fertilization is discussed.  相似文献   

4.
This study describes the post-embryonic growth of the terminal ganglion in Acheta domesticus in terms of volume and cell number. All measurements were made at the beginning of each instar from hatching until the final moult on animals reared under controlled conditions. The terminal ganglion increases about 40-fold in volume from 2 × 106 μ3 in the first instar to 85 × 106 μ3 in the adult. A double logarithmic plot of ganglion volume against body weight shows that the ganglion volume is a function of body weight to the 0.56 power. Initially the neuropile grows at a greater rate than the cortex; in later stages they increase at the same rate. Increase in cell number was determined from serial sections. The total number of cells, based on corrected nuclear counts, increases from 3,400 to 20,000. There is little or no increase in the number of neurons. There are approximately 2,000 association neurons and 100 motor neurons in all stages. The number of glial cells increase from 1,000 to 17,000. Their multiplication rate appears to be related to the increase in neuron volume. Despite the increase in glial cell number, increase in cell volume is primarily responsible for the increase in total volume of the ganglion.  相似文献   

5.
Intergeneric gene transfer mediated by plant protoplast fusion   总被引:1,自引:0,他引:1  
Summary In attempts at somatic transfer of plant genomes of reduced size, X-irradiated leaf protoplasts of parsley (Petroselinum hortense, 2n=22) were fused with cell culture protoplasts of a nuclear albino mutant of carrot (Daucus carota, 2n=18). Introduction of genes from the irradiated parsley nuclei into the carrot genome was shown by the correction of the albino defect and by the appearance of parsley isoenzymes in selected green tissues and plants. The cytological studies provided information on significant deviation from the amphidiploid chromosome number. The high frequency of cells with 2n=19, 2n=38 and regeneration of plants with 2n=19 chromosomes can indicate that the elimination of parsley chromosomes is incomplete. A correlation was found between the lethality of selected tissues and differentiated or undifferentiated stages of the cells.  相似文献   

6.
Baroux C  Fransz P  Grossniklaus U 《Planta》2004,220(1):38-46
Somatic polyploidization is recognized as a means to increase gene expression levels in highly active metabolic cells. The most common mechanisms are endoreplication, endomitosis and cell fusion. In animals and plants the nuclei of multinucleate cells are usually prevented from fusing. Here, we report that the nuclei from the syncytial cyst of the chalazal endosperm of Arabidopsis thaliana (L.) Heynh. are polyploid with some intermediate ploidy levels that cannot be attributed to endoreplication, suggesting nuclear fusion. Analysis of isolated nuclei, together with fluorescent in situ hybridization (FISH), revealed that nuclei from the chalazal endosperm are two or three times bigger than the nuclei from the peripheral endosperm and have a corresponding increase in ploidy. Together with the consistent observation of adjoined nuclei, we propose that nuclear fusion contributes, at least in part, to the process of polyploidization in the chalazal endosperm. Confocal analysis of intact seeds further suggested that free nuclei from the peripheral endosperm get incorporated into the chalazal cyst and likely participate in nuclear fusions.Abbreviations BAC Bacterial artificial chromosome - CZE Chalazal endosperm - DAPI 4,6-Diamino-2-phenylindole - FISH Fluorescent in situ hybridization - NOR Nucleolar organizing region - NCD Nuclear cytoplasmic domain - PEN Peripheral endosperm  相似文献   

7.
The Araceae, a basal-most family of Alismatales that basally diverged subsequent to Acorales in monocot phylogeny, are known to have diverse modes of endosperm development: nuclear, helobial, and cellular. However, the occurrence of nuclear and helobial endosperm development has long been debated. Here, we report a (re-)investigation of endosperm development in Lysichiton, Orontium, and Symplocarpus of the Orontioideae (a basal Araceae), in which nuclear endosperm development was recorded more than 100 years ago. The results show that all three genera exhibit a cellular, rather than nuclear, endosperm development and suggest that the helobial endosperm development reported as an “unmistakable record” from Ariopsis is likely cellular. Thus the Araceae are very likely characterized by cellular endosperm development alone. An extensive comparison with other monocots in light of phylogenetic relationships demonstrates that a plesiomorphic cellular endosperm development is restricted to the three basal monocot orders Acorales, Alismatales, and Petrosaviales, in which evolutionary changes from cellular to nuclear endosperm development occurred twice as major events, once within Alismatales and once as a synapomorphy of the eight remaining monocot orders, including Dioscoreales, Liliales, Asparagales, and Poales, and that helobial endosperm development, which is known for many monocot families, evolved as homoplasy throughout the monocots.  相似文献   

8.
The four synaptonemal complexes (SC) in Meloidogyne nataliei (n=4) have normal, tripartite morphology, although the total width of the SC is only 50 nm. Each SC is attached at both ends to the nuclear envelope and there is no bouquet formation at pachytene. Pairing of homologues is regular but not complete, as there is one region on each bivalent where the SC is not formed. This region may be the fusion point of telomeres of nonhomologous chromosomes, since it is assumed that M. nataliei has been derived from an ancestral strain (n=8) via chromosomal fusions. In each pachytene nucleus there is a nonmembranous, vacuole-like structure of unknown function located in the nucleoplasm adjacent to the nucleolus, and of approximately the same volume as the nucleolus. The fact the SC structure of M. nataliei is strikingly different from that of most Meloidogyne species suggest that M. nataliei may not belong to the same phyletic group as the genus Meloidogyne.  相似文献   

9.
Summary The transparent integument ofJasione montana ovules enables the observation of the nucleolar vacuolar pulsation in egg cell, in secondary nucleus and after the fertilization in zygote, in primary endosperm nucleus and in cells of embryo and endosperm too. The rate of vacuolar pulsation depends on their volume, on the total cell activity and with the endosperm cells on the length of their mitotic cycle. Vacuolar activity can be observed continuously during the developmental cycle of the endosperm cell nucleoli. It starts immediately after the nucleolus formation in telophase, it continues during entire interphase and it ends immediately before the nucleolus disappearence in the late prophase. The pulsation is not interrupted during the nucleoli fusion in prophase.

Das Material für die Arbeit erhielten wir beim Drehen des wissenschaftlichen Filmes E 1751 am IWF in Göttingen. Für fachliche RatschlÄge, Durchführung der Filmaufnahmen und technische Mitarbeit danke ich Herrn Dr. H. K.Galle, Herrn H. H.Heunert und FrÄulein B.Milthaler. Die finanziellen Mittel stellte in dankenswerter Weise die DFG zur Verfügung.  相似文献   

10.
 Cytological details of endosperm development after pollination with irradiated pollen were studied in Actinidia deliciosa (kiwifruit) cultivar Hayward. Pollinations were carried out involving five different sources of pollen (Matua, Tomuri, Burt, Berryman, and fruiting male) irradiated with gamma rays at doses of 700 and 900 Gy. Non-irradiated crosses were used as controls. Irradiated pollen induced development of approximately 25–30% of the ovules. Two types of ovules were observed: (1) with both embryo and endosperm and (2) with endosperm only. No mitotic abnormalities were found in control or irradiated endosperms. Mitotic divisions were regular and nuclei spherical and evenly spaced. However, the cells of irradiated endosperm usually contained low amounts of storage products. Ploidy level of the endosperms was evaluated by nuclear size (volume) with the use of image analyzis. Mean nuclear size in control and irradiated endosperms was 1598.3 and 750.9 μm3, respectively. It is concluded that endosperm produced after pollination with irradiated pollen is autonomous and represents the 2n level. Received: 14 October 1998 / Revision accepted: 10 March 1999  相似文献   

11.
Specific chromatin domains within interphase nuclei are organized in cell type specific distributions and are rearranged in association with changes in cell function. Axotomy leads to changes in gene expression. Dorsal root ganglion (DRG) neurons in vitro are a model for axotomy because they are detached from their axons in preparation for the culturing procedure. In a test of the hypothesis that neurons regrowing in vitro undergo rearrangement of specific chromatin domains, changes in the distribution of centromere-associated kinetochores proteins within DRG neurons were assessed as a function of time in vitro. Comparison of the kinetochore distributions in neurons in situ to those 24 h after placement into culture showed that the mean proportion (±S.E.M.) of kinetochore signals in the karyoplasm decreased from 41.0 ± 1.8% to 28.6 ± 3.3%, while the proportion at the nucleolus increased from 35.2 ± 2.0% to 48.4 ± 2.9%. This indicated redistribution of centromeric domains to the nucleolus. Between 1 day and 16 days in vitro, signals were redistributed to the nuclear periphery, indicated by an increase in the proportion of signals in this nuclear compartment from 23.0 ± 4.3% to 37.6 ± 3.4% and a decrease in the proportion of signals from 48.4 ± 2.9% to 23.0 ± 2.3% at the nucleolus. The results indicate that neurite regrowth following axotomy is associated with changes in nuclear topology. The reorganization that occurs within 24 h is speculated to be associated with a recapitulation of a cytoskeletal development program, while later changes in centromeric distributions may be related to cues elicited by in vitro conditions. © 1996 John Wiley & Sons, Inc.  相似文献   

12.
Chengqi Ao 《Plant biosystems》2019,153(5):673-678
To unravel a low fecundity in Zephyranthes candida (Lindl.) Herb., the development of the endosperm was studied using conventional paraplast section technique. The results show that the endosperm develops normally and comprises four major stages viz. syncytial, cellularization, differentiation and maturation. Both proliferation of antipodal cells and their close contact with the primary endosperm nucleus were observed, which should favor transportation of nutrients and accelerate development of embryo and endosperm. In Z. candida, at least four events of nuclear migration occurred during the course of embryogenesis and endosperm development. The 12.7% structurally and functionally abnormal ovules, along with the 22.3% collapsed and aborted ovules observed accounts for the low fecundity to some extent.  相似文献   

13.
DATTA  P. C. 《Annals of botany》1971,35(2):421-427
From a study of 100 metaphase plates in root-tip squashes and75 fibre-cells in leaf-base squashes, it is found that the chromosomenumber, although having an irregular type of variation, commonlytend to occur as multiples of 15, the haploid number. In developingfibre-cells the tendency of complete endomitosis (resultingin 2n, 4n, and 8n numbers) is stronger than the partial endomitosis(resulting in 5n, 6n, 7n, etc.). Nuclear volumes of fibre-cellsalso roughly correspond to 2K, 4K, and 8K. Significant linearcorrelations between chromosome number and nuclear volume andbetween nuclear volume and fibre-cell volume has been obtained.Thus the development and differentiation of fibres involve completeand partial endomitoses, accompanied by somatic non-disjunctionphenomena.  相似文献   

14.
Abstract

Among Angiosperms, only six species are known to possess the lowest identified chromosome number, i.e. 2n = 4. These plants are the monocotyledons Zingeria biebersteiniana, Colpodium versicolor, Ornithogalum tenuifolium and Rhynchospora tenuis, and the dicotyledons Haplopappus gracilis and Brachyscome dichromosomatica. The low chromosome number may be cytogenetically derived from a different ancestral basic number, characteristic of each genus, by different processes, including tandem fusion or unequal reciprocal translocations with loss of centric fragments. All these plants possess low nuclear 4C DNA contents, ranging from 1.56 pg (R. tenuis) to 8.20 pg (H. gracilis), and they generally display a similar chromosome size and a similar position of the nucleolus organising region (NOR), that is often located in the terminal or subterminal region of the small chromosome pair. All these characteristics could be a consequence of common adaptative mechanisms. Peculiar characteristics within these karyotypes are the holocentric chromosomes of R. tenuis and the presence of B chromosomes in B. dichromosomatica. Plants with a very low chromosome number may be considered to constitute a fascinating “island of interest”; moreover, they represent simple systems helpful for the examination of the structural organisation and evolution of Angiosperm chromosomes.  相似文献   

15.
Selected nuclear and cytoplasmic changes of pollen grains of Hyoscyamus niger during normal gametophytic development and embryogenic development, induced by anther culture, were analyzed and compared ultrastructurally using stereological methods. Potentially embryogenic, uninucleate pollen could be identified within 6 hr of culture by an increased ratio of the volume density of the nucleolar granular zone to the volume density of the fibrillar zone and an increased ratio of dispersed to condensed chromatin in the nucleoplasm. Nonembryogenic pollen in vitro and in vivo possessed prominent nucleolar fibrillar zones and low ratios of dispersed to condensed chromatin. These differences may reflect changes in nuclear activity in potentially embryogenic pollen grains during early stages of culture. Following the first haploid mitosis, in potentially embryogenic pollen the generative cell maintained its large granular nucleolus and high ratio of dispersed to condensed chromatin through its first division to form a proembryoid. The volume fraction of the cytoplasm occupied by mitochondria and plastids and the area fraction occupied by RER and Golgi cisternae differed in the generative cells of potentially embryogenic and nonembryogenic pollen. Those changes only detected in generative cells of potentially embryogenic pollen include: increased area and complexity of cytoplasmic membranes, increased mitochondrial volume, and the presence of plastids at all stages of development. These results support the idea that embryogenic induction of H. niger takes place at the uninucleate stage of development and that subsequent nuclear and cytoplasmic changes are essential for continued sporophytic development.  相似文献   

16.
In angiosperms, interspecific crosses often display hybrid incompatibilities that are manifested as under‐proliferation or over‐proliferation of endosperm. Recent analyses using crosses between Arabidopsis thaliana and its related species with different ploidy levels have shown that interspecific hybridization causes delayed developmental transition and increased mitotic activity in the endosperm. In this study, we investigated endosperm development in interspecific crosses between diploid Oryza species. In a cross between female O. sativa and male O. punctata, we found that the hybrid endosperm was reduced in size and this cross was associated with precocious developmental transition. By contrast, the cross between O. sativa and O. longistaminata generated enlarged hybrid endosperm at the mid‐point of seed development and this cross was associated with delayed developmental transition. Subsequently, the hybrid endosperm displayed a shriveled appearance at the seed maturation stage. We found that the accumulation of storage products and the expression patterns of several marker genes were also altered in the hybrid endosperm. By contrast, the rate of syncytial mitotic nuclear divisions was not significantly affected. The gene OsMADS87 showed a maternal origin‐specific expression pattern in rice endosperm, in contrast to its Arabidopsis homologue PHERES1, which shows paternal origin‐specific expression. OsMADS87 expression was decreased or increased depending on the type of developmental transition change in the hybrid rice endosperm. Our results indicate that one of the interspecies hybridization barriers in Oryza endosperm is mediated by precocious or delayed developmental alterations and de‐regulation of OsMADS87, without change to the rate of syncytial mitotic nuclear division in the hybrid endosperm.  相似文献   

17.
18.
List , Albert , Jr . (Douglass Coll., Rutgers U., New Brunswick, N. J.) Some observations on DNA content and cell and nuclear volume growth in the developing xylem cells of certain higher plants. Amer. Jour. Bot. 50(4): 320–329. Illus. 1963.—The developing metaxylem cells of Acorus calamus roots undergo an over-all growth in cell and nuclear volume that may be expressed roughly as a constant ratio of relative growth rates. Within this over-all growth picture, however, there is a periodicity of both nuclear and cell volume growth. Other plants such as Peltandra, Eleocharis, and Dennstaedtia undergo a similar volume growth. Marsilea tracheary elements have an increase in nuclear number per cell by simultaneous divisions. Arisaema metaxylem nuclei go through a series of DNA doublings correlated with nuclear volume doubling and cell volume increase, at least up to the 16- or 32-ploid level. The cells display some tendency to fall into size classes, expressing a pulsation in growth. A fluctuating alternation or stepwise growth of cell and nucleus appears to describe the data more suitably than the allometric growth equation. In Zea, the cell and nuclear volumes for metaxylem cells fit a fluctuating envelope better than the straight line, and there is again the probability that cell volumes fall into size classes related to nuclear volume class. The DNA content of the nuclei was determined to fall into a frequency distribution having peaks at the 4-, 8-, 16-, and 32-ploid equivalent, agreeing with an earlier report for diploid maize. DNA content was highly correlated with nuclear volume in the over-all growth of the metaxylem cells.  相似文献   

19.
W. Nagl 《Planta》1971,96(2):145-151
Summary Gibberellic acid, injected into maturing ovules of Phaseolus vulgaris, induces 3.2-fold enhancement of the number of additional nucleoli within the endopolyploid endosperm nuclei. The additional nucleoli originate at different sites of polytene chromosome-like chromosome bundles. They contain RNA and are sensitive to actinomycin D. Similar nucleolar bodies are extruded by the main nucleolus. It is assumed that gibberellic acid stimulates the chromosomal and nucleolar RNA synthesis, i.e., the gene activity, in the endosperm nuclei.RNA synthesis was tested by 3H-thymidine it could be seen that gibberellin-treated and gibberellin-treated endosperms were labeled, but those of actinomycin-treated endosperms were not. Using 3H-thymidine it could be seen that gibberellin-treated endosperm nuclei continue to replicate their DNA for a longer period than untreated nuclei. Hence it follows that the treated nuclei become more highly endopolyploid and are capable of functioning longer than the untreated ones.

Mit Unterstützung durch den Fonds zur Förderung der wissenschaftlichen Forschung der Republik Österreich.  相似文献   

20.
Morphological changes in the nuclear degeneration of the synergid (mainly the synergid that receives the pollen tube) and antipodal cells in Triticum aestivum were studied. Although located in the same embryo sac, and derived from the same megaspore, nuclear degeneration of the synergid and antipodal cells differs greatly. Nuclear degeneration in the synergid is characterized by pycnosis, i.e., total chromatin condensation, nuclear deformation and distinct shrinkage in volume, followed by the formation of an irregular and densely stained mass—the degenerated nucleus—while the nucleolus disappears prior to the degradation of chromatin. In contrast, in the nuclear degeneration of antipodal cells, chromatin is only partly condensed and the nuclear volume changes only slightly after the distinct chromatin condensation. Chromatolysis then occurs, i.e., stainable contents disappear while the nuclear envelope is retained. The nucleoli persist after the disappearance of the chromatin. The possible functions of nuclear degeneration of synergid and antipodal cells are discussed, especially with respect to the guidance of pollen tube growth and the proliferation of free-nuclear endosperm. The degeneration of synergids and antipodal cells in T. aestivum are distinct forms of programmed cell death, regarded as cytoplasmic cell death and nuclear degradation in advance of cell death, respectively.  相似文献   

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