共查询到20条相似文献,搜索用时 62 毫秒
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Gibbings JG Cook BP Dufault MR Madden SL Khuri S Turnbull CJ Dunwell JM 《Plant biotechnology journal》2003,1(4):271-285
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Pereira GS Brandão RM Giuliatti S Zago MA Silva WA 《Genetics and molecular research : GMR》2006,5(1):108-114
Serial analysis of gene expression (SAGE) technology produces large sets of interesting genes that are difficult to analyze directly. Bioinformatics tools are needed to interpret the functional information in these gene sets. We present an interactive web-based tool, called Gene Class, which allows functional annotation of SAGE data using the Gene Ontology (GO) database. This tool performs searches in the GO database for each SAGE tag, making associations in the selected GO category for a level selected in the hierarchy. This system provides user-friendly data navigation and visualization for mapping SAGE data onto the gene ontology structure. This tool also provides graphical visualization of the percentage of SAGE tags in each GO category, along with confidence intervals and hypothesis testing. 相似文献
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The impact of SNPs on the interpretation of SAGE and MPSS experimental data 总被引:2,自引:0,他引:2 下载免费PDF全文
Silva AP De Souza JE Galante PA Riggins GJ De Souza SJ Camargo AA 《Nucleic acids research》2004,32(20):6104-6110
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Transcriptomes for serial analysis of gene expression 总被引:1,自引:0,他引:1
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Serial analysis of gene expression in Plasmodium falciparum reveals the global expression profile of erythrocytic stages and the presence of anti-sense transcripts in the malarial parasite 总被引:9,自引:0,他引:9 下载免费PDF全文
Patankar S Munasinghe A Shoaibi A Cummings LM Wirth DF 《Molecular biology of the cell》2001,12(10):3114-3125
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Correct identification of genes from serial analysis of gene expression tag sequences 总被引:3,自引:0,他引:3
SAGE (serial analysis of gene expression) is a remarkable technique for genome-wide analysis of gene expression. It is crucial to understand the extent to which SAGE can accurately indicate a gene or expressed sequence tag (EST) with a single tag. We analyzed the effect of the size of SAGE tag on gene identification. Our observation indicates that SAGE tags are in general not long enough to achieve the degree of uniqueness of identification originally envisaged. Our observations also indicate that the limitation of using SAGE tag to identify a gene can be overcome by converting SAGE tags into longer 3' EST sequences with the generation of longer cDNA fragments from SAGE tages for gene identification (GLGI) method. 相似文献
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The Arabidopsis root transcriptome by serial analysis of gene expression. Gene identification using the genome sequence 总被引:5,自引:0,他引:5
Fizames C Muños S Cazettes C Nacry P Boucherez J Gaymard F Piquemal D Delorme V Commes T Doumas P Cooke R Marti J Sentenac H Gojon A 《Plant physiology》2004,134(1):67-80
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Molecular phenotype of the human oocyte by PCR-SAGE 总被引:17,自引:0,他引:17