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Phosphorylation of E47 as a potential determinant of B-cell-specific activity. 总被引:8,自引:4,他引:4
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S R Sloan C P Shen R McCarrick-Walmsley T Kadesch 《Molecular and cellular biology》1996,16(12):6900-6908
The E2A gene encodes two basic helix-loop-helix proteins designated E12 and E47. Although these proteins are widely expressed, they are required only for the B-lymphocyte lineage where DNA binding is mediated distinctively by E47 homodimers. By studying the properties of deltaE47, an N-terminal truncation of E47, we provide evidence that phosphorylation may contribute to B-cell-specific DNA binding by E47. Two serines N terminal to the deltaE47 basic helix-loop-helix domain were found to be phosphorylated in a variety of cell types but were hypophosphorylated in B cells. Phosphorylating these serines in vitro inhibited DNA binding by deltaE47 homodimers but not by deltaE47-containing heterodimers, such as deltaE47:MyoD. These results argue that hypophosphorylation may be a prerequisite for activity of E47 homodimers in B cells, suggesting the use of an inductive (nonstochastic) step in early B-cell development. 相似文献
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Id proteins in development,cell cycle and cancer 总被引:43,自引:0,他引:43
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