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1.
Abstract: Ineffective, non-infective actinomycetous isolates obtained from actinorhizal nodules of Coriaria nepalensis and Datisca cannabina were identified as Frankia using whole cell fatty acid analysis. The isolates exhibited fatty-acid patterns very similar to those of confirmed Frankia strains from other host plants ( Alnus, Casuarina, Colletia, Comptonia, Elaeagnus and Hippophae ). All Frankia strains, including Coriaria and Datisca isolates, showed fatty-acid profiles very distinct from those of other actinomycetes used as controls ( Actinomyces, Geodermatophilus, Nocardia, Mycobacterium and Streptomyces ). For the genus Frankia , a characteristic pattern of five fatty acids (15:0; 15:1; 16:0 iso; 17:0 and 17:1) was found. These fatty acids comprised 75% or more of the total content. All Frankia strains could be placed into three subgroups. Coriaria isolates were found in the largest subgroup which contained most Frankia strains from other hosts while ineffective strains from Alnus, Elaeagnus and Datisca were distributed in all three subgroups of Frankia . 相似文献
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B. Duarte M. T. Cabrita C. Gameiro A. R. Matos R. Godinho J. C. Marques I. Caçador 《Plant biology (Stuttgart, Germany)》2017,19(2):239-248
- A profound analysis of A. tripolium photochemical traits under salinity exposure is lacking in the literature, with very few references focusing on its fatty acid profile role in photophysiology.
- To address this, the deep photochemical processes were evaluated by Pulse Amplitude Modulated (PAM) Fluorometry coupled with a discrimination of its leaf fatty acid profile.
- Plants exposed to 125–250 mm NaCl showed higher photochemical light harvesting efficiencies and lower energy dissipation rates. under higher NaCl exposure, there is evident damage of the oxygen evolving complexes (OECs). On the other hand, Reaction Centre (RC) closure net rate and density increased, improving the energy fluxes entering the PS II, in spite of the high amounts of energy dissipated and the loss of PS II antennae connectivity. Energy dissipation was mainly achieved through the auroxanthin pathway. Total fatty acid content displayed a similar trend, being also higher under 125–250 mm NaCl with high levels of omega‐3 and omega‐6 fatty acids. The increase in oleic acid and palmitic acid allows the maintenance of the good functioning of the PS II. Also relevant was the high concentration of chloroplastic C16:1t in the individuals subjected to 125–250 mm NaCl, related with a higher electron transport activity and with the organization of the Light Harvesting Complexes (LHC) and thus reducing the activation of energy dissipation mechanisms.
- All these new insights shed some light not only on the photophysiology of this potential cash‐crop, but also highlight its important saline agriculture applications of this species as forage and potential source of essential fatty acids.
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Deguil J Pineau L Rowland Snyder EC Dupont S Beney L Gil A Frapper G Ferreira T 《Traffic (Copenhagen, Denmark)》2011,12(3):349-362
Exposure of pancreatic β cells to long-chain saturated fatty acids (SFA) induces a so-called endoplasmic reticulum (ER) stress that can ultimately lead to cell death. This process is believed to participate in insulin deficiency associated with type 2 diabetes, via a decrease in β-cell mass. By contrast, some unsaturated fatty acid species appear less toxic to the cells and can even alleviate SFA-induced ER stress. In the present study, we took advantage of a simple yeast-based model, which brings together most of the trademarks of lipotoxicity in human cells, to screen fatty acids of various structures for their capacity to counter ER stress. Here we demonstrate that the tendency of a free fatty acid (FFA) to reduce SFA toxicity depends on a complex conjunction of parameters, including chain length, level of unsaturation, position of the double bonds and nature of the isomers (cis or trans). Interestingly, potent FFA act as building blocks for phospholipid synthesis and help to restore an optimal membrane organization, compatible with ER function and normal protein trafficking. 相似文献
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Cione E Senatore V Tucci P Giudetti AM Genchi F Gnoni GV Genchi G 《Journal of bioenergetics and biomembranes》2007,39(2):203-209
All-trans-retinoic acid (atRA) is incorporated covalently into proteins of rat testes mitochondria. In this study, the effect of three
diets with different fatty acid composition on the retinoylation of proteins of rat testes mitochondria has been investigated.
Different groups of rats were fed on a basal diet supplemented with 15% of either coconut oil (CO), olive oil (OO) or fish
oil (FO). We found that, when compared with CO, the binding of retinoic acid was decreased in FO- and OO-fed rats. Mitochondrial
phospholipids composition was differently influenced by dietary treatments; minor changes were observed in fatty acid composition
of phospholipids. Few differences were observed in the Arrhenius plots among the three groups of rats. Kinetic analysis revealed
a decrease in the V
max value in FO- and OO- as compared with CO-fed rats. No difference among the three groups were observed in the K
M
value. The retinoylation reaction was inhibited by 13-cis-RA and 9-cis-RA. 相似文献
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A comprehensive understanding of the lipid and fatty acid metabolic machinery is needed for optimizing production of oils and fatty acids for fuel, industrial feedstocks and nutritional improvement in plants. T-DNA mutants in the poorly annotated Arabidopsis thaliana gene At1g08640 were identified as containing moderately high levels (50-100%) of 16∶1Δ7 and 18∶1Δ9 leaf fatty acids and subtle decreases (5-30%) of 16∶3 and 18∶3 (http://www.plastid.msu.edu/). TLC separation of fatty acids in the leaf polar lipids revealed that the chloroplastic galactolipids monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) were the main lipid types affected by this mutation. Analysis of the inferred amino acid sequence of At1g08640 predicted the presence of a transit peptide, three transmembrane domains and an N-terminal J-like domain, and the gene was named CJD1 for Chloroplast J-like Domain 1. GFP reporter experiments and in vitro chloroplast import assays demonstrated CJD1 is a chloroplast membrane protein. Screening of an Arabidopsis cDNA library by yeast-2-hybrid (Y2H) using the J-like domain of CJD1 as bait identified a plastidial inner envelope protein (Accumulation and Replication of Chloroplasts 6, ARC6) as the primary interacting partner in the Y2H assay. ARC6 plays a central role in chloroplast division and binds CJD1 via its own J-like domain along with an adjacent conserved region whose function is not fully known. These results provide a starting point for future investigations of how mutations in CJD1 affect lipid composition. 相似文献
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Rasheed Faisal Anjum Naser A. Masood Asim Sofo Adriano Khan Nafees A. 《Journal of Plant Growth Regulation》2022,41(5):1891-1904
Journal of Plant Growth Regulation - The salinization of agriculture soils over the globe has become one of the most devastating stresses and is significantly limiting cultivated land area, and... 相似文献
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Interaction between isolation source, cellular fatty acid composition and stress tolerance in Saccharomyces cerevisiae and its subspecies 总被引:1,自引:1,他引:0
Maria Elisabetta Guerzoni Milena Sinigaglia F. Gardini 《Journal of applied microbiology》1993,75(6):588-594
The physiological properties and fatty acid content of 59 strains of Saccharomyces cerevisiae isolated from soft-drink factories, a fruit puree factory, a fuel-alcohol distillery and a winery were compared. Discriminant analysis of the results allocated the strains to four groups according to their source. Resistance to preservatives and temperature stress were correlated with differences in fatty acid composition. The fatty acid C18: 1Δ11, growth at pH 2 and in the presence of 200–600 mg 1-1 benzoate or sorbate, and maximal growth rate at 42°C were characteristics associated with yeasts from particular environments. However, tolerance of thermal stress and content of the C18: 2 fatty acid were associated with subspecies: the former species S. capensis, S. chevalieri , etc. The relative content of C10 : 0, C12 : 0 and C18 : 0 acids varied according to both isolation source and subspecies. 相似文献
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Fructose and copper have been shown independently to influence long chain fatty acid metabolism. Since fructose feeding exacerbates copper deficiency, their possible interaction with respect to tissue long chain fatty acid and lipid composition was studied. Weanling male Sprague-Dawley rats were given diets containing 0.6 or 6 mg/kg copper. The carbohydrate source (627 g/kg) was either fructose or corn starch. After 3 wk, fatty acid profiles and total lipids in heart and liver were analyzed. Copper-deficient rats fed fructose had more severe signs of copper deficiency than those fed starch, according to heart/body wt ratio, hematocrit, and liver copper content. The fatty acid composition of heart and liver triacylglycerol was significantly different between groups, but the changes did not correlate with the severity of copper deficiency. In heart, phosphatidylinositol and phosphatidylserine, arachidonic acid and docosapentaenoic acid (n-6) were increased 193 and 217%, respectively, p<0.05) in rats given the copper-deficient diet containing fructose. Changes in the long chain fatty acids in heart phospholipids may be related to the higher mortality commonly observed in rats fed a copper-deficient diet containing fructose. 相似文献
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Ana Sofia Ferraz Laura Carreto Sandra Tenreiro M. Fernanda Nobre Milton S. da Costa 《Antonie van Leeuwenhoek》1994,66(4):357-363
The polar lipids and fatty acid composition ofThermus aquaticus YT-1 and YS 041,T. filiformis Wai33 A1 and eighteen isolates from New Zealand, several of which are attributed toT. filiformis, were compared to complement the taxonomy of these organisms. The polar lipid patterns were essentially similar in all strains and consisted of one major phospholipid and one major glycolipid. The fatty acid analysis produced three basic groups corresponding toT. filiformis Wai33 A1,T. aquaticus and the third to the other New Zealand strains. The presence of hydroxy fatty acids is reported inThermus spp. for the first time. 相似文献
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龙眼叶片膜脂脂肪酸组分与龙眼耐寒性的关系 总被引:8,自引:0,他引:8
龙眼叶片膜脂不饱和脂肪酸含量和脂肪酸不饱和度与龙眼不同品种的耐寒性呈正相关;在年周期中,不饱和脂肪酸含量的变化与龙眼耐寒性的变化呈现平行关系;龙眼叶片膜脂脂肪酸组分含量反映着龙眼品种间耐寒性遗传上的差异,可作为耐寒性的鉴定指标。 相似文献
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Biotechnology of water and salinity stress tolerance 总被引:1,自引:0,他引:1
Jose M Pardo 《Current opinion in biotechnology》2010,21(2):185-196
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Fatty acids incorporated into cytotoxic T lymphocytes (CTL) during in vitro stimulation can enhance or inhibit the subsequent expression of cell-mediated cytotoxicity, depending on the class of fatty acid. Unsaturated fatty acids enhance cytolysis, whereas saturated fatty acids inhibit it. The effects of fatty acids on cytolysis can be mediated in the absence of cell division, thus eliminating relative clonal amplification or contraction as a basis for the observed effects. Nevertheless, the net result of fatty acid alteration is an increase (unsaturated fatty acids) or decrease (saturated fatty acids) in the frequency, in the treated immune population, of CTL capable of lysing target cells. These observations are best explained by a model in which fatty acid incorporated into cell membrane phospholipids results in a direct recruitment into or out of the pool of CTL within the immune population capable of lysing target cells under the conditions employed to assay cytotoxicity. 相似文献
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The lipid accumulation, fatty acid composition and γ-linolenic acid (GLA) production by 28 strains belonging to Mucorales were investigated. The lipid content varied from 5 to 30% on dry biomass and the percentage of γ-linolenic acid in total intracellular lipid was in a range from 2.5 to 15.4% (w/w). The best yield of γ-linolenic acid (expressed as mg GLA per 1 g biomass) was found for Mucor mucedo CCF – 1384 (28.4) and Cunninghamella echinulata CCF – 103 (25.1). 相似文献
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Somaclonal variation and chilling tolerance improvement in rice: Changes in fatty acid composition 总被引:2,自引:0,他引:2
Pierre Bertin Pol Bullens Jules Bouharmont Jean-Marie Kinet 《Plant Growth Regulation》1998,24(1):31-41
The relationship between chilling tolerance of six rice cultivars – Facagro 57, Facagro 76, Fujisaka 5, Kirundo 3, Kirundo 9 and IR64 -and the fatty acid composition in total lipids, phospholipids, galactolipids and neutral lipids from leaves was studied. Higher double bond index and proportions of linolenic acid in the phospholipid and galactolipid classes were related to cultivar chilling tolerance, but this was not so for the total lipids nor the neutral lipid class. The somaclonal families derived from Facagro 76, Kirundo 3 and Kirundo 9 that showed enhanced chilling tolerance as compared to their original parental cultivar were analyzed for fatty acid composition in phospholipids and galactolipids from leaves. Altered proportions in fatty acid composition in phospholipids, galactolipids or both were found in the somaclonal families derived from Facagro 76 and Kirundo 9, but not from Kirundo 3. These changes most usually resulted in higher double bond index and higher proportions in linoleic and linolenic acids which were related either to lower ratio of C16 to C18 fatty acids or to higher unsaturation in the C18 fatty acid fraction. Different mechanisms thus seem to be implicated in the altered fatty acid composition of somaclones, which may be related to the chilling tolerance improvement of some somaclonal families. 相似文献
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Role of salicylic acid on physiological and biochemical mechanism of salinity stress tolerance in plants 总被引:1,自引:0,他引:1
Salinity stress is one of the major abiotic stresses affecting plant growth and productivity globally. In order to improve the yields of plants growing under salt stress bear remarkable importance to supply sustainable agriculture. Acclimation of plants to salinized condition depends upon activation of cascade of molecular network involved in stress sensing/perception, signal transduction, and the expression of specific stress-related genes and metabolites. Isolation of salt overly sensitive (SOS) genes by sos mutants shed us light on the relationship between ion homeostasis and salinity tolerance. Regulation of antioxidative system to maintain a balance between the overproduction of reactive oxygen species and their scavenging to keep them at signaling level for reinstating metabolic activity has been elucidated. However, osmotic adaptation and metabolic homeostasis under abiotic stress environment is required. Recently, role of phytohormones like Abscisic acid, Jasmonic acid, and Salicylic acid in the regulation of metabolic network under osmotic stress condition has emerged through crosstalk between chemical signaling pathways. Thus, abiotic stress signaling and metabolic balance is an important area with respect to increase crop yield under suboptimal conditions. This review focuses on recent developments on improvement in salinity tolerance aiming to contribute sustainable plant yield under saline conditions in the face of climate change. 相似文献
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G. A. Farris Milena Sinigaglia Marilena Budroni Maria Elisabetta Guerzoni 《Letters in applied microbiology》1993,17(5):215-219
Eleven strains belonging to two physiological races of Saccharomyces cerevisiae endowed with different abilities of forming films at air-liquid interfaces were analysed in relation to cell fatty acid composition and cell hydrophobicity. Extensive individual differences in fatty acid profiles were observed both in the film and in the non-film phase. The ability of the cells to form a floating film seems to be an implicit strain character associated with an elevated unsaturation level and a mean chain length of fatty acid residues, as well as cellular hydrophobicities higher than those shown by non-film-forming strains belonging to the same species. 相似文献
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Peter J. H. Jones Lin Lin Leah G. Gillingham Haifeng Yang Jaclyn M. Omar 《Journal of lipid research》2014,55(12):2655-2664
N-acylethanolamines (NAEs) are endogenous lipid-signaling molecules involved in satiety and energetics; however, how diet impacts circulating NAE concentrations and their downstream metabolic actions in humans remains unknown. Objectives were to examine effects of diets enriched with high-oleic canola oil (HOCO) or HOCO blended with flaxseed oil (FXCO), compared with a Western diet (WD), on plasma NAE levels and the association with energy expenditure and substrate oxidation. Using a randomized controlled crossover design, 36 hypercholesterolemic participants consumed three isoenergetic diets for 28 days, each containing 36% energy from fat, of which 70% was HOCO, FXCO, or WD. Ultra-performance liquid chromatography-MS/MS was used to measure plasma NAE levels and indirect calorimetry to assess energy expenditure and substrate oxidation. After 28 days, compared with WD, plasma oleoylethanolamide (OEA) and alpha-linolenoyl ethanolamide (ALEA) levels were significantly increased in response to HOCO and FXCO (P = 0.002, P < 0.001), respectively. Correlation analysis demonstrated an inverse association between plasma OEA levels and percent body fat (r = −0.21, P = 0.04), and a positive association was observed between the plasma arachidonoyl ethanolamide (AEA)/OEA ratio and android:gynoid fat (r = 0.23, P = 0.02), respectively. Results suggest that plasma NAE levels are upregulated via their dietary lipid substrates and may modulate regional and total fat mass through lipid-signaling mechanisms. 相似文献
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《Environmental and Experimental Botany》2007,59(3):276-282
Crop salt tolerance is generally assessed as the relative yield response to increasing root zone salinity, expressed as soil (ECe) or irrigation water (ECw) electrical conductivity. Alternatively, the dynamic process of salt accumulation into the shoot relative to the shoot biomass has also been considered as a tolerance index. These relationships are graphically represented by two intersecting linear regions, which identify (1) a specific threshold tolerance, at which yield begins to decrease, and (2) a declining region, which defines the yield reduction rate. Although the salinity threshold is intuitively a critical parameter for establishing plant salt tolerance, we focused our interest on physiological modifications that may occur in the plant at salinity higher than the so-called tolerance threshold. For this purpose, we exposed hydroponically grown tomato plants to eight different salinity levels (EC = 2.5 (non-salinized control); 4.2; 6.0; 7.8; 9.6; 11.4; 13.2; 15.0 dS m−1). Based on biomass production, water relations, leaf ions accumulation, leaf and root abscisic acid and stomatal conductance measurements, we were able to identify a specific EC value (approximately 9.6 dS m−1) at which a sharp increase of the shoot and root ABA levels coincided with (1) a decreased sensitivity of stomatal response to ABA; (2) a different partitioning of Na+ ions between young and mature leaves; (3) a remarkable increase of the root-to-shoot ratio. The specificity and functional significance of this response in salt stress adaptation is discussed. 相似文献