共查询到4条相似文献,搜索用时 0 毫秒
1.
S. Kurosawa M. Harada Yoshihiro Shinomiya Hiroshi Terao Kikuo Nomoto 《Cancer immunology, immunotherapy : CII》1996,43(1):31-38
The effect of a local injection with a streptococcal preparation OK432 on the antitumor vaccination with tumor cells was
investigated. Natural killer (NK) cells, which were detected by anti-NK1.1 monoclonal antibody (mAb), increased in the peritoneal
exudate cells after an intraperitoneal (i.p.) injection with syngeneic B16 melanoma cells. Furthermore, a concurrent i.p.
injection with OK432 efficiently sustained the locally infiltrating NK cells. The OK432 treatment also sustained the augmented
NK and lymphokine-activated killer activities in the peritoneal exudate cells. This treatment also increased the ability of
the locally infiltrating NK cells to produce interferon γ in response to the tumor cells. In addition, the concurrent i.p.
injection with OK432 in combination with the tumor cells enhanced the capacity of the spleen cells to turn into anti-(B16
melanoma) cytotoxic T lymphocytes after in vitro restimulation. This augmenting effect of OK432 was dependent on NK cells.
Moreover, the concurrent injection with OK432 at the time of antitumor vaccination significantly enhanced the protective immunity
against B16 melanoma at the rechallenge. Taken together, these findings indicate that a concurrent local injection with OK432
in combination with tumor cells efficiently augments the antitumor vaccination effect, in part, by sustaining the locally
infiltrating activated NK cells.
Received: 6 March 1996 / Accepted: 30 May 1996 相似文献
2.
Enhancement of the photodynamic antitumor effect by streptococcal preparation OK-432 in the mouse carcinoma 总被引:3,自引:0,他引:3
Uehara M Sano K Wang ZL Sekine J Ikeda H Inokuchi T 《Cancer immunology, immunotherapy : CII》2000,49(8):401-409
Biological response modifier antitumor effects are enhanced by the activation of the host defense mechanisms. We have investigated
the antitumor effect of photodynamic therapy (PDT) and/or local administration of a biological response modifier, the streptococcal
preparation OK-432, on transplanted NR-S1 mouse squamous cell carcinoma. Hematoporphyrin oligomers (20 mg/kg body weight)
were used to photosensitize PDT. A pulsed Nd:YAG dye laser, tuned at 630 nm, was used as the light source. The laser power
was 15 mJ cm−2 pulse−1, and the irradiation time was 40 min. The photosensitizer was injected intraperitoneally 48 h before laser irradiation. Where
used, OK-432 was injected into the tumor either 3 h prior to PDT or immediately afterwards. The antitumor effects were evaluated
48 h after each protocol by (a) estimating the area of tumor necrosis (%) in hematoxylin/eosin-stained specimens, and (b)
bromodeoxyuridine immunohistochemistry. Furthermore, the tumor sizes were evaluated 3, 7 and 10 days after each protocol,
and the survival time after each protocol was evaluated as well. The anti-tumor effect of PDT was enhanced by administration
of OK-432 3 h before PDT, whereas the administration of OK-432 immediately after PDT did not potentiate a PDT antitumor effect.
Treatment with OK-432 alone had little effect on tumors. Photodynamic therapy in combination with local administration of
OK-432 3 h before PDT is considered to be a useful treatment modality.
Received: 23 July 1999 / Accepted: 31 May 2000 相似文献
3.
The present model study explores the chemistry of methionine complexes and ternary methionine-guanine adducts formed by trans-[PtCl2(NH3)2] (1) and antitumor trans-[PtCl2(NH3)quinoline] (2) using 1D (1H, 195Pt) and 2D NMR spectroscopy. Compound 2 was substitution inert in reactions with N-acetyl-lmethionine [AcMet(H)]. Reactions of trans-[PtCl(NO3)(NH3)quinoline] (5) ("monoactivated" 2) with AcMetH in water and acetone at various stoichiometries point to Pt(II)-S binding that requires prior activation of
the Pt-Cl bond by labile oxygen donors. Trans-[PtCl{AcMet(H)-S}(NH3)quinoline](NO3) (6) and trans-[Pt{AcMet(H)-S}2(NH3)quinoline](NO3)2 (7) were isolated from these mixtures. At high [Cl–], AcMet(H) is displaced from 7, giving 6. Frozen stereodynamics in 6 at the thioether-S and slow rotation about the Pt-Nquinoline bond result in four spectroscopically distinguishable diastereomers. 1H NMR spectra of 7 show faster exchange dynamics due to mutual trans-labilization of the sulfur donors. Substitution of chloride in trans-[PtCl(9-EtGua)(NH3)L]NO3 (L=NH3, 3; L=quinoline, 4; 9-EtGua=9-ethylguanine, which mimics the first DNA binding step of 1 and 2) by methionine-sulfur proceeded ca. 2.5 times slower for the quinoline compound. Both reactions, in turn, proved to be ca.
4 times faster than binding of a second nucleobase under analogous conditions. From the resulting mixtures the ternary adducts
trans-[Pt(AcMet-S)(9-EtGua-N7)(NH3)L](NO3, Cl) (L=NH3, 8; L=quinoline, 9) were isolated. A species analogous to 9 formed in a rapid reaction between 6 and 5′-guanosine monophosphate (5′-GMP). From NMR data an AMBER-based solution structure of the resulting adduct, trans-[Pt(AcMet-S)(5′-GMP-N7)(NH3)quinoline] (10), was derived. The unusual reactivity along the N7-Pt-S axis in 8–10 resulted in partial release of both 9-EtGua and AcMet at high [Cl–]. Possible consequences of the kinetic and structural effects (e.g., trans effect of sulfur, steric demand of quinoline) observed in these systems with respect to the (trans)formation of potential
biological cross-links are discussed.
Received: 25 May 1998 / Accepted: 6 August 1998 相似文献