子宫内膜癌组织中uPA及Cath-D的表达及意义(英文)

目的:检测子宫内膜癌组织中尿激酶型纤溶酶原激活物(uPA)及组织蛋白酶(Cath-D)的表达并探讨相关性及其临床意义。方法:采用免疫组织化学方法(PV-6000二步法)检测31例子宫内膜癌组织(内膜癌组),17例子宫内膜增生组织(增生组)及10例正常子宫内膜组织(对照组)中uPA及Cath-D的表达,并研究其相关性。结果:1.内膜癌组中uPA和Cath-D的表达均高于增生组及对照组中的表达,差异均有统计学意义(P0.05);在增生组中的表达与对照组差异无统计学意义(P0.05)。2.uPA和Cath-D的阳性表达与子宫内膜癌的临床病理分期、组织学分级及肌层浸润深度有关,差异均具有统计学意义(P0.05)。3.内膜癌组中uPA与Cath-D的表达呈正相关(r=0.673,P0.05)。结论:uPA和Cath-D在子宫内膜癌发生发展及侵袭转移过程中起着协同作用,Cath-D可诱导产生活化的uPA,促进癌细胞的浸润转移,因此,两者的联合检测可有助于成为判断子宫内膜癌的发展及预后的重要指标。     

子宫内膜癌中TGF-β_1及受体TβRI的表达及其临床意义

目的:研究转化生长因子(TGF-β_1)及其Ⅰ型受体(TβRI)在子宫内膜癌组织中的表达及其临床意义。方法:采用RT-PCR方法检测36例子宫内膜癌及31例正常子宫内膜组织中TGF-β_1及TβRI基因的表达水平,分析其与临床病理参数之间的关系。结果:子宫内膜癌组织中TGF-β_1mRNA的表达明显高于正常组织(P<0.05),而TβRI mRNA的表达水平明显低于正常内膜组织(P<0.05)。TGF-β_1mRNA及TβRImRNA在子宫内膜癌中的表达均与肿瘤的临床分期、组织学分级、肌层浸润深度、淋巴结转移呈显著性相关(P<0.05),差别有统计学意义。结论:TGF-β_1及其Ⅰ型受体TβRI在子宫内膜癌的发生、发展中可能起重要作用,检测TGF-β_1及TβRI对评价子宫内膜癌的转移及预后有重要价值。     

子宫内膜癌C-erbB-2和TTF-1的表达及其与预后的关系

目的:探讨子宫内膜癌组织中C-erbB-2和TTF-1的表达及其与患者临床预后的关系。方法:应用免疫组化S-P法检测正常子宫内膜组织、子宫内膜不典型增生组织、子宫内膜癌组织中C-erbB-2与TTF-1的表达,并分析二者与子宫内膜癌临床病理特征及患者临床预后的关系。结果:子宫内膜癌组织、子宫内膜不典型增生组织、正常子宫内膜组织中C-erbB-2的阳性表达率分别为56.65%、36.67%、16.67%(P0.05),TTF-1的的阳性表达率分别为33.75%、53.33%、70.00%(P0.05)。C-erbB-2的阳性表达与子宫内膜癌的临床病理分期、组织分化、肌层浸润及有无淋巴结转移呈显著正相关(P0.05),与其病理类型无显著相关性(P0.05)。TTF-1的阳性表达与子宫内膜癌的病理分期、组织分化、肌层浸润深度负相关(P0.05),但与有无淋巴结转移及病理类型均无显著相关性(P0.05)。C-erbB-2和TTF-1在子宫内膜癌组织中表达无明显相关性(P=0.303)。子宫内膜癌中C-erbB-2阳性表达者五年生存率(80.90%)显著低于C-erbB-2阴性表达者(93.80%)(P0.05),TTF-1阳性表达者五年生存率(87.70%)略高于TTF-1阴性表达者(85.70%),但差异无统计学意义(P0.05)。结论:子宫内膜癌组织中C-erbB-2的表达显著上调,而TTF-1的表达明显下调,二者表达异常均参与了子宫内膜癌的发生和发展,且C-erbB-2的表达上调与子宫内膜癌患者的不良预后相关。     

eIF-4E 和MMP9 在子宫内膜癌中的表达及临床意义

目的:探讨真核翻译起始因子4E(e IF-4E)和基质金属蛋白酶9(MMP9)在子宫内膜癌组织中的表达及其临床意义。方法:应用免疫组化法检测e IF-4E和MMP9在16例正常子宫内膜组织、10例增生子宫内膜组织及42例子宫内膜癌组织中的表达,分析其与子宫内膜癌临床病理之间的关系,并研究二者的相关性。结果:e IF-4E与MMP9在三组组织中的表达阳性率逐渐增加,三组间的差异有统计学意义(P0.05),两两比较,正常内膜和内膜癌之间的差异有统计学意义(P0.0167)。e IF-4E和MMP9的表达与子宫内膜癌的FIGO分期、组织学分级及淋巴结转移具有显著相关性(P0.05);子宫内膜癌组织中e IF-4E与MMP9的阳性表达呈正相关(r=0.717,P0.05)。结论:e IF-4E和MMP9在子宫内膜癌组织中表达显著上调,随FIGO分期的增加、组织学分级的增高及有淋巴结转移而增高,可能与子宫内膜癌的发生发展相关,二者的联合检测对于评估子宫内膜癌患者的病情及预后有重要的参考意义。     

整合素α1和嗜铬素A在子宫内膜癌中的检测表达

目的:探讨整合素α1和嗜铬素A与子宫内膜癌的相关性。方法:收集哈尔滨医科大学附属第四医院病理科2006年5月至2008年10月存档的113例石蜡包埋子宫内膜标本并进行分类,应用免疫组织化学方法检测内膜组织中整合素α1和嗜铬素A的定位及表达情况。结果:在正常子宫内膜、单纯增生、复杂增生、不典型增生及子宫内膜癌中,整合素α1的表达随病变加重呈上升趋势,正常子宫内膜与增生性内膜及内膜癌比较有显著性差异(P0.05,P0.001),增生性内膜与内膜癌比较有显著性差异(P0.05),在内膜癌中,整合素α1表达与细胞分化程度相关;嗜铬素A表达亦呈上升趋势,正常子宫内膜与增生内膜及内膜癌比较有显著性差异(P0.05,P0.05),但增生性内膜与内膜癌比较无统计学意义(P0.05)。结论:整合素α1的过表达与子宫内膜癌的发生相关,嗜铬素A与子宫内膜癌的相关性仍有待于进一步研究。     

SPARC 在子宫内膜癌组织中的表达及意义

目的:探讨富含半胱氨酸的酸性分泌型蛋白(secreted protien,acidic and rich in cysteine,SPARC)在子宫内膜癌组织中的表达及其临床意义。方法:应用组织芯片技术研究SPARC在正常子宫内膜、增生的子宫内膜和子宫内膜癌组织中的表达情况。结果:SPARC在正常子宫内膜、增生的子宫内膜和子宫内膜癌组织中的阳性表达率分别为96.55%、76.79%、59.50%,差异具有统计学意义(P<0.05)。在子宫内膜癌中,SPARC的表达强度与手术-病理分期、组织学分级、肌层浸润深度和淋巴结转移相关,差异具有统计学意义(P<0.05)。结论:SPARC表达的缺失与子宫内膜癌的发生、发展密切相关,检测SPARC可为子宫内膜癌的早期诊断、进一步治疗及预后判断提供一定的理论依据。     

子宫内膜癌中PTEN与PI3K-Akt通路的表达及意义

目的:研究PTEN与PIK3-Akt通路在子宫内膜癌中的表达及临床意义。方法:采用免疫组化技术检测正常子宫内膜组织(24例)、子宫内膜样腺癌组织(55例)及子宫内膜浆液性癌组织(26例)中PTEN、P13Kp110α及p-AKTSer473的表达情况,分析其表达与组织病理参数的关系。结果:免疫组化结果显示:子宫内膜癌的PTEN表达缺失率,P13Kp110α及p-AKTSer473表达阳性率均高于正常子宫内膜组织,且正常子宫内膜组织与子宫内膜样腺癌组织比较有统计学意义(P0.05);随着肌层浸润深度增加,PTEN表达缺失率增加(P0.05)。结论:PTEN的表达缺失及P13Kp110α与p-AKTSer473的表达可能与子宫内膜癌的发生发展有关,尤其是在子宫内膜样腺癌的发生发展机制中占重要地位。     

子宫内膜癌中TGF-β1及受体TβRI的表达及其临床意义

目的：研究转化生长因子（TGF-β1）及其Ⅰ型受体（TβRI）在子宫内膜癌组织中的表达及其临床意义。方法：采用RT-PCR方法检测36例子宫内膜癌及31例正常子宫内膜组织中TGF-β1及TβRI基因的表达水平,分析其与临床病理参数之间的关系。结果：子宫内膜癌组织中TGF-β1mRNA的表达明显高于正常组织（P〈0.05）,而TβRI mRNA的表达水平明显低于正常内膜组织（P〈0.05）。TGF-β1mRNA及TβRImRNA在子宫内膜癌中的表达均与肿瘤的临床分期、组织学分级、肌层浸润深度、淋巴结转移呈显著性相关（P〈0.05）,差别有统计学意义。结论：TGF-β1及其Ⅰ型受体TβRI在子宫内膜癌的发生、发展中可能起重要作用,检测TGF-β1及TβRI对评价子宫内膜癌的转移及预后有重要价值。     

凋亡抑制蛋白Survivin在增生性子宫内膜及子宫内膜癌中的表达

目的　研究凋亡抑制蛋白Survivin在正常子宫内膜、增生性子宫内膜及子宫内膜癌中的表达 ,探讨Survivin蛋白在子宫内膜癌发生发展中的作用及其作为预后判断因子的可行性。方法　应用免疫组织化学S P法 ,检测 15例正常子宫内膜、 2 6例增生性子宫内膜及 33例子宫内膜腺癌中Survivin蛋白的表达 ,并结合临床病理特点进行分析。结果　Sur vivin蛋白的阳性表达率在正常子宫内膜 ,增生性子宫内膜及子宫内膜癌中呈上升趋势。正常子宫内膜仅在增生期有微弱的表达 ,而分泌期及绝经期子宫内膜表达全为阴性 ;而Survivin在子宫内膜癌中及不典型增生中的阳性表达率分别为87 88%和 70 0 % ,均明显高于正常内膜 (P <0 0 5 ) ,且两者的过表达率均高于单纯和复合型增生及正常内膜 (P <0 0 5 ) ,但子宫内膜癌中与不典型增生中的Survivin表达率及过表达率均无明显差异。子宫内膜癌中Survivin的表达强度与组织学分级及手术病理分期明显相关 (P <0 0 5 ) ,但与肌层浸润无关。结论　Survivin作为凋亡相关因子和细胞周期调节因子 ,可能参与了与子宫内膜癌的发生发展 ,其过度表达与预后不良相关 ,其检测可为子宫内膜癌的早期诊断、辅助治疗及预后判断提供理论依据。     

Claudin-7和β-catenin在正常、增生和恶性子宫内膜组织中的表达和意义

目的恶性肿瘤转移过程与细胞间粘附作用的丧失密切相关。本研究旨在探讨细胞间粘附分子claudin-7和β-catenin在正常、增生和恶性子宫内膜组织中的表达及其在子宫内膜癌中的临床病理意义。方法应用免疫组化检测30例正常增生期子宫内膜、27例非典型增生子宫内膜和43例子宫内膜癌组织中claudin-7和β-catenin的表达情况,统计分析它们与子宫内膜癌病理分级、临床分期、浸润深度及淋巴结转移等指标之间的关系,并对二者表达之间的关系进行探讨。结果在正常增生期子宫内膜、非典型增生子宫内膜和子宫内膜癌中,claudin-7的表达依次减低,阳性率分别为96.7%,66.7%和39.5%,三组之间具有显著差异（P〈0.05）;β-catenin在非典型增生子宫内膜和子宫内膜癌组织中的异位表达率分别为40.7%和74.4%,均显著高于正常增生期子宫内膜（3.3%）（P〈0.01）,子宫内膜癌组织中β-catenin的异位表达率显著高于非典型增生子宫内膜（P〈0.01）。在子宫内膜癌中,claudin-7的表达与临床分期、浸润深度和淋巴结转移有关（P〈0.01）;β-catenin的异位表达与病理分级、临床分期、浸润深度和淋巴结转移有关（P〈0.01）;Claudin-7的表达和β-catenin的异位表达呈显著负相关（r=-0.813,P〈0.01）。结论Claudin-7和β-catenin的异常表达与子宫内膜癌的发生发展有关,并且在此过程中claudin-7逐渐缺失以及β-catenin的异位表达可能对肿瘤的侵袭和转移产生一定作用。     

Structure and function of S-adenosylhomocysteine hydrolase

In mammals, S-adenosylhomocysteine hydrolase (AdoHcyase) is the only known enzyme to catalyze the breakdown of S-adenosylhomocysteine (AdoHcy) to homocysteine and adenosine. AdoHcy is the product of all adenosylmethionine (AdoMet)-dependent biological transmethylations. These reactions have a wide range of products, and are common in all facets of biometabolism. As a product inhibitor, elevated levels of AdoHcy suppress AdoMet-dependent transmethylations. Thus, AdoHcyase is a regulator of biological transmethylation in general. The three-dimensional structure of AdoHcyase complexed with reduced nicotinamide adenine dinucleotide phosphate (NADH) and the inhibitor (1′R, 2′S, 3′R)-9-(2′,3′-dihyroxycyclopenten-1-yl)adenine (DHCeA) was solved by a combination of the crystallographic direct methods program, SnB, to determine the selenium atom substructure and by treating the multiwavelength anomalous diffraction data as a special case of multiple isomorphous replacement. The enzyme architecture resembles that observed for NAD-dependent dehydrogenases, with the catalytic domain and the cofactor binding domain each containing a modified Rossmann fold. The two domains form a deep active site cleft containing the cofactor and bound inhibitor molecule. A comparison of the inhibitor complex of the human enzyme and the structure of the rat enzyme, solved without inhibitor, suggests that a 17° rigid body movement of the catalytic domain occurs upon inhibitor/substrate binding.     

Land,livestock, and livelihoods: Changing dynamics of gender,caste, and ethnicity in a Nepalese Village

Over the past 10 years, Ghusel VDC, Lalitpur District has moved from primarily subsistence agriculture into the wider cash economy aided by the Small Farmers' Development Program (SFDP), which provides credit to farmers mainly for the purchase of buffalo for milk production, and by the National Dairy Corporation, which supports local dairy cooperatives. Analysis reveals that buffalo-keeping and milk sales are increasing the well-being of many households, while at the same time creating new inequalities in gender roles and responsibilities, greater inequities between Brahmin and Tamang residents in Ghusel, and placing pressures on the ecosystem for increased supplies of fodder and fuelwood. Evidence suggests that there is critical, need for attention to the social, and particularly gender-based, implications of maintaining livestock for milk sales and to the ecological underpinnings of this livelihood system.     

Tuning of electronic properties of one-dimensional cyano-bridged Cu-Ni, Cu-Pd, and Cu-Pt bimetallic assemblies by stereochemistry of ligands

Preparations, XPS and electronic spectroscopy, and magnetism of seven new one-dimensional cyano-bridged coordination polymers, chiral [Cu(RR-chxn)₂][Pd(CN)₄] · 2H₂O (1), [Cu(trans-chxn)₂][M(CN)₄] · 2H₂O (2, 4, and 6 for M = Pd, Ni, and Pt), and [Cu(cis-chxn)₂][M(CN)₄] · 2H₂O (3, 5, and 7 for M = Pd, Ni, and Pt) (RR-chxn = cyclohexane-(1R,2R)-diamine, trans-chxn = racemic trans-cyclohexane-(1,2)-diamine, and cis-chxn = racemic cis-cyclohexane-(1,2)-diamine) have been reported in view of tuning of their electronic properties by stereochemistry of chxn ligands and metal-substitution. Comparison of Cu 2p_1/2 and 2p_3/2 peaks of XPS and broad d-d bands around 18 000 cm⁻¹ of electronic spectra are described systematically for 1-7. Variable-temperature magnetic measurement shows that complexes 1-7 indicate weak antiferromagnetic interactions via cyano-bridges. Because of semi-coordination coupled with pseudo Jahn-Teller elongation and electrostatic interaction for 1, the axial Cu-N coordination bond distances of 2.330(7) and 3.092(8) Å are considerably longer than those of equatorial ones in the range from 2.016(6) to 2.030(6) Å. The former bond distances of 1 are intermediate values among the related Ni (2.324(6) and 3.120(8) Å) and Pt (2.34(1) and 3.09(1) Å) complexes.     

Influence of Fluoride on Rat Kidney Antioxidant System: Effects of Methionine and Vitamin E

The aim of the study has been to determine and compare the influence upon the kidney antioxidative system, exercised by administration of vitamin E, and vitamin E in combination with methionine, under conditions of oxidative stress induced by sodium fluoride. The experiment was carried out on Wistar FL rats (adult males) that, for 35 days, were administered water, NaF, NaF with vitamin E, or vitamin E with methionine (doses: 10 mg NaF/kg of body mass/24 h, 3 mg vitamin E per 10 μl per rat for 24 h, 2 mg methionine per rat for 24 h). The influence of administered sodium fluoride and antioxidants upon the antioxidative system in kidney was examined by analyzing the concentration of malondialdehyde (MDA) and the activity of the most important antioxidative enzymes (SOD, total and both its isoenzymes, GPX, GST, GR, and CAT). The studies carried out confirmed the disadvantageous effect of the administered dose of NaF upon the antixodiative system in rats (increase in the concentration MDA, decrease activity of all antioxidative enzymes). The administration of vitamin E increased the activity of studied enzymes with the exception of glutathione reductase GR; it also reduced the procesess of lipid peroxidation. It has been found that combined doses of vitamin E and methionine were most effective in inhibiting lipid peroxidation processes. The results confirmed the antioxidative properties of methionine.     

Gamma-glutamylcysteine protects ergothioneine-deficient Mycobacterium tuberculosis mutants against oxidative and nitrosative stress

Mycobacterium tuberculosis (M.tb.), the causative agent of tuberculosis (TB), cannot synthesize GSH, but synthesizes two major low molecular weight thiols namely mycothiol (MSH) and ergothioneine (ERG). Gamma-glutamylcysteine (GGC), an intermediate in GSH synthesis, has been implicated in the protection of lactic acid bacteria from oxidative stress in the absence of GSH. In mycobacteria, GGC is an intermediate in ERG biosynthesis, and its formation is catalysed by EgtA (GshA). GGC is subsequently used by EgtB in the formation of hercynine-sulphoxide-GGC. In this study, M.tb. mutants harbouring unmarked, in-frame deletions in each of the fives genes involved in ERG biosynthesis (egtA, egtB, egtC, egtD and egtE) or a marked deletion of the mshA gene (required for MSH biosynthesis) were generated. Liquid chromatography tandem mass spectrometry analyses (LC-MS) revealed that the production of GGC was elevated in the MSH-deficient and the ERG-deficient mutants. The ERG-deficient ΔegtB mutant which accumulated GGC was more resistant to oxidative and nitrosative stress than the ERG-deficient, GGC-deficient ΔegtA mutant. This implicates GGC in the detoxification of reactive oxygen and nitrogen species in M.tb.     

Reorientation of Microfibrils and Microtubules at the Outer Epidermal Wall of Maize Coleoptiles During Auxin-Mediated Growth

Auxin-mediated elongation growth of isolated subapical coleoptile segments of maize (Zea mays L.) is controlled by the extensibility of the outer cell wall of the outer epidermis (Kutschera et al., 1987). Here we investigate the hypothesis that auxin controls the extensibility of this wall by changing the orientation of newly deposited microfibrils through a corresponding change in the orientation of cortical microtubules. On the basis of electron micrographs it is shown that cessation of growth after removal of the endogenous source of auxin is correlated with a relative increase of longitudinally orientated microfibrils and microtubules at the inner wall surface. Conversely, reinduction of growth by exogenous auxin is correlated with a relative increase of transversely orientated microfibrils and microtubules at the inner wall surface. These changes can be detected 30–60 min after the removal and addition of auxin, respectively. The functional significance of directional changes of newly desposited wall microfibrils for the control of elongation growth is discussed.     

眶额叶微量注射谷氨酸和γ-氨基丁酸对胃运动的影响及其机制探讨

目的:研究眶额叶(OFC)的谷氨酸(Glu)和γ-氨基丁酸(GABA)含量变化对胃运动的影响及其调节神经机制。方法:实验采用了大鼠眶额叶微量注射给药,结合核团损毁的方法,以记录胃内压,统计胃收缩幅度作为胃运动变化的指标。结果:①OFC注射Glu可显著降低胃收缩幅度,损毁杏仁核后可反转该效应,胃收缩幅度显著增强;损毁蓝斑核后,Glu的作用无显著性变化。②OFC注射GABA可显著增强胃的收缩幅度,损毁蓝斑核后消除该效应;损毁杏仁核后,胃收缩幅度进一步增强。结论:外源性增加OFC区Glu含量导致的抑胃效应可能是通过增强了杏仁核的经常性抑胃作用而引起的;而增加OFC区GABA的含量引起的胃运动增强与蓝斑核密切相关。     

Highly flexible O,O′,N ligands and their Fe, Ni, Cu and Zn complexes

Three new chiral ligands bearing an O,O′,N donor set (O_methoxyO_hydroxyN_pyridine) were synthesised and coordinated to Fe^III, Fe^II, Ni^II, Cu^II and Zn^II to yield complexes with the general formula [M(OON)Cl_x]_y. While the pyridine N and the hydroxy O atoms coordinate strongly to all applied metal ions, the methoxy donor seems not to be involved in coordination, although some evidence for a weak interaction between O_Me and the Zn^II were found in NMR spectra. In the bidentate O′,N coordination mode the new ligands exhibit several coordination geometries as analysed in the solid compounds by XRD, EXAFS and EPR and in solution by UV-Vis absorption, cyclic voltammetry, EXAFS, EPR or NMR spectroscopy.     

Expression of mRNA for PACAP and its receptors in intra- and extra-adrenal human pheochromocytomas and their relationship to catecholamine synthesis

Purpose: Pituitary adenylate cyclase-activating polypeptide (PACAP), a member of the secretin/glucagons/vasoactive intestinal peptide family, induces the expression of catecholamine-synthesizing enzymes in adrenal medullary cells. In addition, PACAP and its receptor have been detected in human pheochromocytoma tissues, though it is not yet known whether PACAP enhances the expression of genes encoding catecholamine-synthesizing enzymes. To address this question, we analyzed PACAP, PACAP receptor, and tyrosine hydroxylase (TH) and phenylethanolamine-N-methyltransferase (PNMT) mRNAs in pheochromocytomas. Methods: The levels of the mRNA for PACAP and vasoactive intestinal peptide (VIP), and their receptors, and for TH and PNMT were measured by RT-PCR or real-time PCR analysis, and the concentrations of catecholamines were measured by HPLC in 24 intra-adrenal and six extra-adrenal pheochromocytomas. Results: mRNA expression of PACAP and its receptor VPAC1R were detected in many pheochromocytomas (24/30 and 29/30, respectively), but mRNA expression of the PAC1R and VPAC2R receptor subtypes were detected in only one of six extra-adrenal pheochromocytomas. PACAP mRNA expression correlated with TH (p=0.0018) and PNMT (p=0.05) mRNA expression, as well as epinephrine (p=0.0342) levels in 16 intra-adrenal pheochromocytomas. Conclusion: Our findings support a possible role for PACAP in the regulation of expression of genes encoding catecholamine-synthesizing enzymes in intra-adrenal pheochromocytomas.     

邻苯二甲酸二丁酯对翡翠贻贝抗氧化酶及脂质过氧化水平的影响

实验室条件下,研究了不同浓度邻苯二甲酸二丁酯(DBP)长期胁迫(15 d)对翡翠贻贝内脏团和外套膜抗氧化酶(超氧化物歧化酶SOD、过氧化氢酶CAT)及脂质过氧化(LPO)水平(以MDA含量表示)的影响,以及受胁迫翡翠贻贝在清洁海水中恢复阶段上述生化指标的变化特征.结果表明:胁迫阶段,0.5和2.5 mg.L-1DBP下翡翠贻贝内脏团SOD活性表现为先抑制后逐渐恢复,12.5和62.5 mg.L-1下则持续受到显著抑制;不同浓度组CAT活性均明显被抑制.LPO水平明显升高.外套膜中,2.5 mg.L-1下SOD活性受到持续诱导,其他浓度组则先被抑制,后随曝露时间延长逐渐被诱导;各浓度组CAT的变化波动较大,没有明显规律;而LPO水平明显升高.净化恢复阶段,12.5和62.5 mg.L-1DBP胁迫下的内脏团SOD和CAT活性恢复较慢,其LPO水平随时间延长逐渐恢复至对照组水平;外套膜中SOD活性呈持续升高趋势,CAT活性和LPO水平则随时间延长恢复到对照组水平.