Components of partial resistance to Septoria nodorum in winter wheat

Components of partial resistance to Septoria nodorum were investigated in 10 cultivars of winter wheat having similar field resistances. The components measured were infection frequency, latent period, size, shape and rate of growth of lesions, spore production and its rate of increase. Latent period was found to be lognormally distributed. Some of the components of resistance were found to be significantly different between cultivars. Cluster analysis also showed that cultivars could be distinguished on the basis of their components of resistance. Principal components analysis indicated that resistance could be broken down into four underlying factors, three of which could be readily interpreted. The measurements of the components of resistance were combined in a model, the r-index, based on Van der Plank's r. The amount of variation between cultivars cast some doubt on the predictive value of the index but all the cultivar values were well within the range bounded by two ‘synthetic’ cultivars made up of combinations of either the most resistant or the most susceptible components. It is considered that the r-index has potential in screening for field resistance. The possibility of incorporating the most resistant-type components into one cultivar is discussed. The use of cultivar mixtures containing cultivars having similar field resistances is also discussed in the light of the variability found in this study.     

Incidence and effects of Septoria nodorum on wheat cultivars

Wheat cultivars were grown in two field trials in which half the plots were artificially inoculated with Septoria nodorum. There were differences between cultivars in the degree of 5. nodorum infection on flag leaves and on ears. Hybrid 46, Maris Huntsman and Maris Widgeon were among those with the least infection, and Maris Ranger and Maris Templar among those with the most. Severity of infection was positively correlated with both short stature and earliness of maturity. Infection on leaves was positively correlated with infection on ears. Inoculation reduced yield by decreasing two of its components, grain number per ear and 1000-grain weight. A very early maturing cultivar, Sterling, sustained no yield loss despite severe infection; for the remaining cultivars, yield loss was positively correlated with degree of infection.     

Antifungal compounds in winter wheat resistant and susceptible to Septoria nodorum

The content of benzoxazinone compounds in Septoria nodorum resistant and susceptible wheat cultivars fell with time, reaching an undetectable level 3–4 wk after germination. The compounds were absent from field-grown plants extracted at growth stages 2–3, 6–7, 10, 10.5 and 11. Additional antifungal compounds were detected in healthy and diseased field-grown plants of both cultivars at all growth stages, although qualitative and quantitative differences in inhibitory activity were small and their role in resistance remains open to discussion.     

Effects of alien cytoplasm substitution on the response of wheat cultivars to Septoria nodorum

The effects of alien cytoplasm substitution on the response of wheat to Septoria nodorum were studied, using alloplasmic series of two cultivars, Chris and Selkirk. In general, cytoplasmic substitution caused unidirectional effects on Septoria-response, alloplasmic lines of both cultivars expressing lower levels of partial resistance (in leaf and head tissue) but higher levels of yield tolerance than the corresponding euplasmic line. The reduced resistance in alloplasmics was closely associated with reduced incubation periods of Septoria infection in both leaf and head tissue. Cytoplasmic substitution resulted in increased yield tolerance to Septoria-infection in both the non-tolerant Selkirk and the relatively tolerant Chris. Unlike their effects on partial resistance, specific cytoplasms exerted similar effects on tolerance in the two parental cultivars, several cytoplasms of the D plasmatype being particularly effective in increasing Septoria-tolevance. The potential for the development of Septoria-toterant cultivars by the incorporation of alien cytoplasms is discussed, in view of the observed neutral effects of D plasmatype cytoplasms on other agronomic traits.     

A bioassay for predicting the resistance of wheat leaves to Septoria nodorum

Plants of the winter wheat cv. Bounty were inoculated with Septoria nodorum and then assayed for ergosterol. A detached leaf technique was used in which leaf segments were incubated on agar containing benzimidazole. Four levels of a conidial suspension inoculum were applied using a measured droplet technique. Ergosterol in plant extracts was measured using HPLC and its identity confirmed by co-injection with pure ergosterol as an internal standard. The extracts were also assayed by silica-gel thin layer chromatography with the sterol being visualised with rhodamine; the presence of ergosterol in the material from the two higher disease level treatments was confirmed by analysing the eluted spots in a spectrophotometer. Additional confirmation of ergosterol was obtained using gas-liquid chromatography comparing a mixture of known plant sterols with a sample from the diseased leaf tissue. The ergosterol assay was found to be very sensitive and offers a high level of reproducibility. It would therefore appear that such assays could be of value in breeding programmes when it is necessary to screen wheat cultivars for their reaction to S. nodorum or other fungal pathogens.     

A method for assessing the efficacy of seed disinfectants for the control of seed-borne Septoria nodorum on wheat

The most rigorous tests of the effectiveness of seed disinfectants for the control of Septoria nodorum on wheat can be made by growing seedlings from treated seeds in the glasshouse at an air temperature of 12 °C and a soil moisture content of 30% of the maximum water holding capacity. In carrying out such tests assessments were made of the percentage of diseased seedlings and of the disease index. Based on the results obtained a schedule of testing is recommended.     

Inheritance of resistance to leaf and glume blotch caused by Septoria nodorum Berk. in winter wheat

Sixteen crosses between eight winter wheat cultivars were screened for resistance to Septoria nodorum leaf and glume blotch in the F₁ and F₄ generations using artificial inoculation in the field. The F₁ of most crosses showed dominance for susceptibility on both ear and leaf. The effects of general combining ability were of similar magnitude as the effects for specific combining ability. On the basis of the phenotypic difference of the parents, no prediction was possible about the amount and the direction of genetic variance in the segregating populations. The variation observed in this study both within and among the segregating populations suggests a quantitative inheritance pattern influencing the expression of the two traits. The components of variance between F₂ families within a population were as high as (for S. nodorum blotch on the ear) or higher (for S. nodorum blotch on the leaf) than those between populations. Therefore, strong selection within a few populations may be as effective to obtain new resistant genotypes as selection in a large number of populations. In almost all crosses, progenies were found that were more resistant than the better parent. Thus transgression breeding may be a tool to breed for higher levels of resistance to S. nodorum blotch. Highly resistant genotypes were found even in combination with two susceptible parents. The genetic source for Septoria resistance is probably broader than is generally assumed and could be used to improve S. nodorum resistance by combination breeding followed by strong selection in large populations. Received: 18 January / Accepted: 30 April 1999     

Predisposing effects of Eyespot (Pseudocercosporella herpotrichoides) on Septoria nodorum infection of winter wheat

All isolates of PeniciUium simplicissimum, P. verrucosum var. cyclopium, P. brevicompactum, P. multicolor, P. oxalicum, P. paxilli, Botrytis cinerea, and of a Gliocladium sp. obtained from necrotic virus-tested narcissus twin-scales previously dtoped in benomyl were tolerant to 1000 μg/ml of this fungicide in agar. Every necrotic twin-scale examined was infected with at least one of these species. The first two species were the most frequently isolated and a similar range of species infected different narcissus clones or different cultivars. It is argued that the use of benomyl during the twin-scaling programme should be discontinued as soon as a suitable alternative fungicide is found.     

Identification of molecular markers for resistance to Septoria nodorum blotch in durum wheat

The development of Septoria nodorum blotch-resistant cultivars has become a high priority objective for durum wheat breeding programs. Marker-assisted selection enables breeders to improve selection efficiency. In order to develop markers for resistance to Septoria nodorum blotch, a set of F₅ recombinant inbred lines, derived from the crosses Sceptre/3–6, Sceptre/S9–10 and Sceptre/S12–1, was developed based on the F₂-derived family method. Two RAPD markers, designated UBC521₆₅₀ and RC37₅₁₀, were detected by bulked segregant analysis and located approximately 15 and 13.1 centiMorgans (cM) from the resistance gene snbTM, respectively. A SCAR marker was also successfully developed for marker-assisted selection in breeding programs based on the sequence of the RAPD marker UBC521₆₅₀. This is the first report of DNA-based markers linked to resistance for Septoria nodorum blotch in durum wheat. Received: 8 March 2000 / Accepted: 25 June 2000     

In vitro screening for resistance against Septoria nodorum blotch in wheat

This study was carried out to develop an in vitro test for the identification of genotypes resistant to Septoria nodorum blotch. The basis for this project was a previous study in which a crude extract of S. nodorum was used as a selective agent (Keller et al. 1994). It was possible to distinguish resistant and susceptible cultivars in an in vitro test with zygotic embryos. In our project we wanted to test whether this in vitro test can also be used to detect resistant and susceptible genotypes in early segregating populations. Specific crosses between eight winter wheat lines showing contrasting resistance reaction for S. nodorum blotch on leaves and ears were made. The resistance level of both leaf and ear was evaluated after artificial inoculation in the field for the parental lines, the F₁ progenies, as well as for segregating F₃ and F₄ populations. In addition, this plant material was tested in vitro using methods similar to those described by Keller et al. (1994), i.e. culturing immature zygotic embryos and mature seeds on selective media. A good agreement between in vitro screening and field resistance on the ear was found for the parental lines, the F₁ and F₄ generation but not for the F₃ generations. This leads to the conclusion that the in vitro screening might be integrated into wheat breeding programs. Populations showing a high susceptibility to the pathogen metabolites in vitro could be discarded. Another promising implementation for wheat breeding would be the screening of advanced breeding material or candidate partners in a crossing program for resistance on the ear. However, the in vitro screening is not precise enough to select single plants in early segregating populations. Received: 18 January 1999 / Accepted: 30 April 1999     

The reaction of wheat genotypes to Septoria tritici

Seedling leaves, flag leaves, culms and heads of wheat genotypes were evaluated under glasshouse and field conditions for reaction to Septoria tritici. Parameters used for resistance screening were the incubation and latent periods, disease and sporulation levels, and 1000 grain weight. Significant correlations were obtained between reactions of different plant organs and between field and glasshouse tests; significant interspecific and intraspecific differences occurred for all screening parameters. Triticum aestivum varieties showed a wide range of disease reaction ranging from the high resistance of Elite Lepeuple, Maris Dove, Maris Ensign, Chalk and Tommy to the high susceptibility of Rothwell Sprite, Sovereign, Cardinal, Maris Ranger and Maris Templar.     

Effects of inoculation with Septoria nodorum on yield components of spring wheat

Spring wheat, cv. Kolibri, was artificially inoculated with spore suspensions of Septoria nodorum, from the time of full expansion of the third leaf to the end of flowering and the effect on yield components recorded. Most components were reduced by increasing severity of infection and grain weight losses of 56 and 35% were produced on single-stemmed and tillering plants respectively, by the most severe infection. The effects on components of yield differed considerably between the main stems and the tillers and an increase in grain number on moderately infected tillers partly compensated for reductions in yield on the main stem.     

Ornithine decarboxylase of Stagonospora (Septoria) nodorum is required for virulence toward wheat

A knockout strain of Stagonospora (Septoria) nodorum lacking the single ornithine decarboxylase (ODC) allele has been created by targeted gene replacement. A central region of the S. nodorum ODC gene was isolated by polymerase chain reaction using degenerate oligonucleotides and used to probe a lambda genomic library. The gene was sequenced and the encoded ODC protein sequence was shown to be similar to those from other fungi. The functionality of the S. nodorum ODC was confirmed by complementation of an Aspergillus nidulans mutant (puA) strain devoid of ODC activity, restoring growth in the absence of exogenous polyamines. Sporulation of the transformants was reduced suggesting abberant regulation of the S. nodorum gene in A. nidulans. Transformation-mediated gene replacement was used to create strains which were auxotrophic for putrescine and lack ODC coding sequences. Pathogenicity studies on these mutants showed that they are greatly reduced in virulence compared with non-disrupted transformants. This confirms that the strains carrying an ODC disruption cannot obtain sufficient polyamines from the host plant for normal growth and, thus, that fungal ODC may be a suitable target for chemical intervention.     

Predisposition to Septoria nodorum as a result of take-all (Gaeumannomyces graminis) infection of wheat

In a series of experiments excised leaves from take-all infected wheat plants and from control plants were inoculated with Septoria nodorum. Larger lesions, more lesions/leaf and more pycnidia/unit area of lesion were produced from take-all plants. Significant effects of predisposition were demonstrated when only 3% of the area of the total root system was infected by take-all. Microscopical investigations revealed that germ-tubes of S. nodorum grew more rapidly on leaves from take-all plants, but the time of penetration was not affected. It is proposed that the observed effects of predisposition arose because more germ-tubes produced successful infections and host tissue was more rapidly colonised. The importance of these results for the epidemiology of glume blotch is discussed.     

The effect of the plasmon on the inheritance of resistance to Septoria nodorum Berk. in soft wheat

The genetic control of resistance to Septoria nodorum in common wheat line Transec have been investigated. There have been concluded that high level of resistance of this line is connected with the presence in its genome of partially recessive gene which is located on wheat-rye translocation T4BS.4BL-2RL or closely linked with it. Plant plasmon can have an influence on inheritance of resistance to Septoria blotch in hybrid progeny.     

Factors Affecting the Germination of Septoria nodorum Pycnidiospores

Most pycnidiospores of Septoria nodorum were released from pycnidia into water in 30 min, more than 50% being released in 10 min. The viability of pycnidiospores in aqueous suspension, assessed by germination on a selective agar medium, decreased more rapidly in daylight than in darkness but no spores germinated after 50 h. The presence of cirrhus extract stimulated germination of pycnidiospores in suspension.