山葵的离体快速繁殖

1　植物名称　山葵 (Ｅｕｔｒｅｍａｗａｓａｂｉ)日本品种岛根 3号。2　材料类别　无菌萌发的种子苗。3　培养条件　基本培养基为自行设计的“贵山”(代号ＧＳ)配方 [其大量元素组分为 :每Ｌ含(ＮＨ4) 2 ＳＯ40 .32 1 ｇ、ＫＮＯ31 .2 81 ｇ、ＫＨ2 ＰＯ40 .2 0 0ｇ、ＣａＣｌ2 · 2Ｈ2 Ｏ 0 .887ｇ和ＭｇＳＯ4· 7Ｈ2 Ｏ0 .746ｇ ,铁盐、微量元素和维生素组分均与ＭＳ配方相同 ]。种子萌发培养基为 :( 1 )ＧＳ 6 ＢＡ 0 .2ｍｇ·Ｌ- 1 (单位下同 ) ;芽增殖培养基为 :( 2 )ＧＳ 6 ＢＡ 0 .4;生根培养基为 :( 3)ＧＳ ＩＢＡ 0 .4。蔗糖…     

金百合的离体快速繁殖

1　植物名称　金百合 (Ｌｉｌｉｕｍｔｒｏｍｐｅｔｅｎ)。2　材料类别　带腋芽的幼嫩茎段。3　培养条件　以ＭＳ为基本培养基。 (1 )芽诱导培养基 :ＭＳ 6 ＢＡ 0 .6～ 1 .0ｍｇ·Ｌ-1(单位下同 ) ＮＡＡ 0 .3～ 0 .4;(2 )增殖培养基 :ＭＳ 6 ＢＡ 0 .5～ 1 .0 ＮＡＡ 0 .1～ 0 .2 ;(3)生根培养基 :ＭＳ ＮＡＡ 0 .3～ 0 .5。培养基含蔗糖 30ｇ·Ｌ-1、琼脂 9ｇ·Ｌ-1,ｐＨ 5 .8。培养温度2 2～ 2 5℃ ,光照度 1 5 0 0ｌｘ ,光照时间 1 2ｈ·ｄ-1。4　生长与分化情况4.1　愈伤组织及芽的诱导　取金百合花序下端幼嫩的带腋芽茎段 ,…     

牛奶子的组织培养及植株再生

1　植物名称　牛奶子 (Ｅｌａｅａｇｎｕｓｕｍｂｅｌｌａｔａ) ,又称甜枣、羊奶子。2　材料类别　幼苗的下胚轴。3　培养条件　 ( 1 )ＭＳ 6 ＢＡ 1ｍｇ·Ｌ- 1 (单位下同 ) 2 ,4 Ｄ 3 ＩＡＡ 0 .5 ＣＨ 30 0 ;( 2 )ＭＳ 6 ＢＡ2 2 ,4 Ｄ 2 ＮＡＡ 0 .5 ＣＨ 30 0 ;( 3)ＭＳ 6 ＢＡ3 2 .4 Ｄ 1 ＩＢＡ 0 .5 ＣＨ 30 0 ;( 4 )ＭＳ 6 ＢＡ1 .5 ＩＡＡ 0 .5;( 5)ＭＳ 6 ＢＡ 1 .5 ＮＡＡ 0 .5;( 6)ＭＳ 6 ＢＡ 1 .5 ＩＢＡ 0 .5;( 7)ＭＳ 6 ＢＡ 0 .2 ＩＡＡ 0 .5 ＧＡ 0 .5;( 8)ＭＳ 6 ＢＡ 0 .2 ＩＡＡ 0 .1 ＧＡ…     

特早熟杏的组织培养与快速繁殖

1　植物名称　杏 (Ｐｒｕｎｕｓａｒｍｅｎｉａｃａ)特早熟品种“红荷包”。2　材料类别　盛花后 45ｄ的自然授粉胚、顶芽、带腋芽的茎段。3　培养条件　培养胚的基本培养基 :( 1 )改良ＳＨ ,成分为 :大量元素ＮＨ4ＮＯ361 0ｍｇ·Ｌ- 1 (单位下同 )、ＫＮＯ32 2 4 0、ＣａＣｌ2 ·2Ｈ2 Ｏ 2 0 0、ＭｇＳＯ4·7Ｈ2 Ｏ40 0、ＫＨ2 ＰＯ435 0 ＭＳ微量元素 ＳＨ的有机物 ＢＡ 1 .0 ＩＡＡ 1 .0 4 %蔗糖。成苗培养基 :( 2 )ＴＭ培养基 0 .0 5 %ＡＣ。诱导分化培养基 :( 3)ＭＳ培养基 ＩＢＡ 0 .0 1 6 ＢＡ 0 .2 5 ;( 4 )ＭＳ培养基 …     

毛刺槐的组织培养和植株再生

1　植物名称　毛刺槐 (Ｒｏｂｉｎｉａｈｉｓｐｉｄａ) ,别名毛洋槐、江南槐。2　材料类别　带腋芽的茎段。取自本校校园里的 1 0～ 2 0年生毛刺槐的当年生新梢。3　培养条件　培养基 :( 1 )ＭＳ 6 ＢＡ 2 .0ｍｇ·Ｌ-1(单位下同 ) ＮＡＡ 0 .0 5 ;( 2 )ＭＳ 6 ＢＡ 2 .0 ＮＡＡ 0 .1 ;( 3)ＭＳ 6 ＢＡ 1 .0 ＮＡＡ 0 .0 5 ;( 4)ＭＳ 6 ＢＡ 1 .0 ＮＡＡ 0 .1 ;( 5 )ＭＳ 6 ＢＡ 0 .5 ＮＡＡ 0 .1 ;( 6 )ＭＳ 6 ＢＡ 0 .5 ＮＡＡ 0 .0 5 ;( 7)ＭＳ。以上培养基均附加蔗糖 30 ｇ·Ｌ-1、琼脂 7ｇ·Ｌ-1,ｐＨ6 .0～ 6 .…     

PAS反应在显示真菌中的应用和体会

ＰＡＳ反应 (Ｐｅｒｏｄｉｃ Ａｃｉｄ Ｓｃｈｉｆｆｒｅａｅｔｉｏｎ)属于糖类的组织化学反应 ,它不仅在对糖原、粘蛋白、粘多糖及含碳水化合物的物质有特异性 ,而且对真菌的显示亦十分理想。现将ＰＡＳ显示真菌的体会予以介绍 :1、材料和方法1.ｌ材料选取外检中食道、胃、鼻腔、上颌窦、肺、皮肤、指 (趾 )甲等组织ＨＥ染色切片中疑有真菌感染者5 0例。重新切片。1.2　改良Ｓｃｈｉｆｆ试剂的配制 ,碱性品红 0 5ｇ加1ｍｏｌ/Ｌ盐酸 15ｍｌ,振荡使之溶解 ,再加 0 6 %偏重亚硫酸钠 85ｍｌ。紧盖瓶塞 ,振荡后在室温下放置 2 4ｈ ,溶液呈草…     

橡皮树的组织培养及快速繁殖

1　植物名称　橡皮树 (Ｆｉｃｕｓｅｌａｓｔｉｃａ)。2　材料类别　茎尖。3　培养条件　培养基 :(1 )ＭＳ ＧＡ 0 .1ｍｇ·Ｌ- 1(单位下同 ) ＩＢＡ 0 .5 6 ＢＡ 1 .0 ;(2 )ＳＨ ＧＡ0 .1 ＩＢＡ 0 .2 6 ＢＡ 1 .0 ;(3 ) 1 /2ＭＳ ＩＡＡ 1 .5。培养基 (1 )、(2 )每升加蔗糖或白糖 3 0 ｇ ,琼脂 7.5ｇ ;培养基 (3 )每升加蔗糖或白糖 1 5 ｇ ,琼脂 7.5 ｇ ;ｐＨ值 5 .86 .0 ,高压灭菌。培养温度 2 5 2 8℃ ,每天光照 1 0 1 2ｈ ,光照度 2 0 0 0ｌｘ。4　生长与分化情况4.1　无菌材料的获得　从盆栽生长健壮、无病虫害的橡皮树植株…     

Glycine—SDS—PAGE测定小分子多肽分子量

小分子多肽分子量的测定 ,目前多采用Ｔｒｉｃｉｎｅ -ＳＤＳ -ＰＡＧＥ系统[1～ 3 ] ,但系统中的Ｔｒｉｃｉｎｅ价格昂贵 ,加之电泳时间较长 ( 3～ 4ｈ) ,为此 ,作者经过实验摸索 ,建立了Ｇｌｙｃｉｎｅ -ＳＤＳ -ＰＡＧＥ测定小分子多肽的方法。1　材料和方法表 1　制胶配方成分分离胶 (ｍｌ)浓缩胶 (ｍｌ)分离胶单体母液 1 3 3 -浓缩胶单体母液 -0 23 2ｍｏｌ/ＬＴｒｉｓ-ＨＣｌ 1 3 3 -(ｐＨ8 8)0 5ｍｏｌ/ＬＴｒｉｓ-ＨＣｌ -0 3 8(ｐＨ6 8)尿素 1 44ｇ -Ｈ2 Ｏ 0 40 910 %ＳＤＳ 40 μｌ 15 μｌ10 %ＡＰＳ 2 5 μｌ 12 μｌＴ…     

大花蕙兰的组织培养和快速繁殖

1　植物名称　大花蕙兰 (Ｃｙｍｂｉｄｉｕｍ ｇｒａｎｄｉｆｌｏ ｒｕｍ)。2　材料类别　茎段。3　培养条件　 (1 )原球茎诱导增殖培养基 :ＭＳ +6 ＢＡ 0 .5ｍｇ·Ｌ- 1 (单位下同 ) +ＮＡＡ 1 .0。 (2 )原球茎分化培养基 :ＭＳ +6 ＢＡ 2 .0 +ＮＡＡ 1 .0 +香蕉泥 1 5 0 ｇ·Ｌ- 1 。 (3 )生根培养基 ,1 /2ＭＳ +ＮＡＡ1 .0。ｐＨ 5 .6～ 5 .8,蔗糖 3 0 ｇ·Ｌ- 1 、琼脂 5 ｇ·Ｌ- 1固化培养 ,培养温度 (2 5± 2 )℃ ,环境湿度 6 0 %～80 % ,光照度 2 5 0 0ｌｘ ,每天光照 1 2ｈ。4　生长与分化情况4.1　启动培养　取大花蕙兰茎段 ,流…     

山葵的组织培养和快速繁殖

1　植物名称　山葵 (Ｅｕｔｒｅｍａｗａｓａｂｉ)品种达摩(种子购自云南 )。2　材料类别　秋播苗的顶芽或侧芽。3　培养条件　启动培养基 :( 1 )ＭＳ + 6 ＢＡ 0 .2ｍｇ·Ｌ- 1 (单位下同 ) +琼脂 7ｇ·Ｌ- 1 +蔗糖 2 0 ｇ·Ｌ- 1 。增殖培养基 :( 2 )ＭＳ + 6 ＢＡ 4+ＮＡＡ 0 .1 +蔗糖 30ｇ·Ｌ- 1 ;( 3)ＭＳ + 6 ＢＡ 0 .2 +ＫＴ 0 .2 +ＮＡＡ 0 .0 5 +琼脂 7ｇ·Ｌ- 1 +蔗糖 30ｇ·Ｌ- 1 。 ( 4 )ＭＳ + 6 ＢＡ 4+ＮＡＡ 0 .1 +琼脂 7ｇ·Ｌ- 1 +蔗糖 30ｇ·Ｌ- 1 。生根培养基 :( 5 )ＭＳ +ＮＡＡ 0 .1 +琼脂 7ｇ·Ｌ- 1 +蔗糖 30ｇ·…     

Investigation of the mechanism of temperature sensitivity of the electroreceptors of ampullae of lorenzini

In experiments on Black Sea skates (Raja clavata), the potential of the receptor epithelium of the ampullae of Lorenzini and spike activity of single nerve fibers connected to them were investigated during electrical and temperature stimulation. Usually the potential within the canal was between 0 and –2 mV, and the input resistance of the ampulla 250–400 k. Heating of the region of the receptor epithelium was accompanied by a negative wave of potential, an increase in input resistance, and inhibition of spike activity. With worsening of the animal's condition the transepithelial potential became positive (up to +10 mV) but the input resistance of the ampulla during stimulation with a positive current was nonlinear in some cases: a regenerative spike of positive polarity appeared in the channel. During heating, the spike response was sometimes reversed in sign. It is suggested that fluctuations of the transepithelial potential and spike responses to temperature stimulation reflect changes in the potential difference on the basal membrane of the receptor cells, which is described by a relationship of the Nernst's or Goldman's equation type.I. P. Pavlov Institute of Physiology, Academy of Sciences of the USSR, Leningrad. I. M. Sechenov, Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of the USSR, Leningrad. Pacific Institute of Oceanology, Far Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostok. Translated from Neirofiziologiya, Vol. 12, No. 1, pp. 67–74, January–February, 1980.     

Principles of organization and evolution of systems of regulation of functions

Evolution of living organisms is closely connected with evolution of structure of the system of regulations and its mechanisms. The functional ground of regulations is chemical signalization. As early as in unicellular organisms there is a set of signal mechanisms providing their life activity and orientation in space and time. Subsequent evolution of ways of chemical signalization followed the way of development of delivery pathways of chemical signal and development of mechanisms of its regulation. The mechanism of chemical regulation of the signal interaction is discussed by the example of the specialized system of transduction of signal from neuron to neuron, of effect of hormone on the epithelial cell and modulation of this effect. These mechanisms are considered as the most important ways of the fine and precise adaptation of chemical signalization underlying functioning of physiological systems and organs of the living organism