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1.
Klinfelter syndrome was first described in adult males with gynecomastia, azoospermia and hypergonadotropic hypogonadism. Children with the 47, XXY karyotype demonstrate few clinical findings so Klinefelter syndrome is rarely diagnosed until adult life. Besides children who have been diagnosed during prenatal genetic testing, in infancy a male with 47, XXY (or variants: 46, XY-47, XXY; 48, XXXY; 48, XXYY, 49, XXXXY) may be found while undergoing evaluation of micropenis, hypospadias, cryptorchidism or facial anomalies. The older child may present with learning disabilities, behavior disorders or tall stature. At the time of puberty, the clinical picture includes small testes, gynecomastia and an eunuchoid habitus. Early diagnosis of Klinefelter syndrome must be performed since it has been demonstrated that early treatment with androgens may ameliorate many aspects of the clinical symptoms and attenuate or prevent behavioral and psychiatric disorders associated with 47, XXY males.  相似文献   

2.
Our previous work using a melanoma progression model composed of melanocytic cells (melanocytes, primary and metastatic melanoma samples) demonstrated various deregulated genes, including a few known lncRNAs. Further analysis was conducted to discover novel lncRNAs associated with melanoma, and candidates were prioritized for their potential association with invasiveness or other metastasis‐related processes. In this sense, we found the intergenic lncRNA U73166 (ENSG00000230454) and decided to explore its effects in melanoma. For that, we silenced the lncRNA U73166 expression using shRNAs in a melanoma cell line. Next, we experimentally investigated its functions and found that migration and invasion had significantly decreased in knockdown cells, indicating an essential association of lncRNA U73166 for cancer processes. Additionally, using naïve and vemurafenib‐resistant cell lines and data from a patient before and after resistance, we found that vemurafenib‐resistant samples had a higher expression of lncRNA U73166. Also, we retrieved data from the literature that indicates lncRNA U73166 may act as a mediator of RNA processing and cell invasion, probably inducing a more aggressive phenotype. Therefore, our results suggest a relevant role of lncRNA U73166 in metastasis development. We also pointed herein the lncRNA U73166 as a new possible biomarker or target to help overcome clinical vemurafenib resistance.  相似文献   

3.
The inference of gene regulatory networks is a key issue for genomic signal processing. This paper addresses the inference of probabilistic Boolean networks (PBNs) from observed temporal sequences of network states. Since a PBN is composed of a finite number of Boolean networks, a basic observation is that the characteristics of a single Boolean network without perturbation may be determined by its pairwise transitions. Because the network function is fixed and there are no perturbations, a given state will always be followed by a unique state at the succeeding time point. Thus, a transition counting matrix compiled over a data sequence will be sparse and contain only one entry per line. If the network also has perturbations, with small perturbation probability, then the transition counting matrix would have some insignificant nonzero entries replacing some (or all) of the zeros. If a data sequence is sufficiently long to adequately populate the matrix, then determination of the functions and inputs underlying the model is straightforward. The difficulty comes when the transition counting matrix consists of data derived from more than one Boolean network. We address the PBN inference procedure in several steps: (1) separate the data sequence into "pure" subsequences corresponding to constituent Boolean networks; (2) given a subsequence, infer a Boolean network; and (3) infer the probabilities of perturbation, the probability of there being a switch between constituent Boolean networks, and the selection probabilities governing which network is to be selected given a switch. Capturing the full dynamic behavior of probabilistic Boolean networks, be they binary or multivalued, will require the use of temporal data, and a great deal of it. This should not be surprising given the complexity of the model and the number of parameters, both transitional and static, that must be estimated. In addition to providing an inference algorithm, this paper demonstrates that the data requirement is much smaller if one does not wish to infer the switching, perturbation, and selection probabilities, and that constituent-network connectivity can be discovered with decent accuracy for relatively small time-course sequences.[1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31]  相似文献   

4.
Cyclopropane fatty acids (CPAs) are desirable as renewable chemical feedstocks for the production of paints, plastics, and lubricants. Toward our goal of creating a CPA-accumulating crop, we expressed nine higher plant cyclopropane synthase (CPS) enzymes in the seeds of fad2fae1 Arabidopsis (Arabidopsis thaliana) and observed accumulation of less than 1% CPA. Surprisingly, expression of the Escherichia coli CPS gene resulted in the accumulation of up to 9.1% CPA in the seed. Coexpression of a Sterculia foetida lysophosphatidic acid acyltransferase (SfLPAT) increases CPA accumulation up to 35% in individual T1 seeds. However, seeds with more than 9% CPA exhibit wrinkled seed morphology and reduced size and oil accumulation. Seeds with more than 11% CPA exhibit strongly decreased seed germination and establishment, and no seeds with CPA more than 15% germinated. That previous reports suggest that plant CPS prefers the stereospecific numbering (sn)-1 position whereas E. coli CPS acts on sn-2 of phospholipids prompted us to investigate the preferred positions of CPS on phosphatidylcholine (PC) and triacylglycerol. Unexpectedly, in planta, E. coli CPS acts primarily on the sn-1 position of PC; coexpression of SfLPAT results in the incorporation of CPA at the sn-2 position of lysophosphatidic acid. This enables a cycle that enriches CPA at both sn-1 and sn-2 positions of PC and results in increased accumulation of CPA. These data provide proof of principle that CPA can accumulate to high levels in transgenic seeds and sets the stage for the identification of factors that will facilitate the movement of CPA from PC into triacylglycerol to produce viable seeds with additional CPA accumulation.Modified fatty acids (mFAs; sometimes referred to as unusual fatty acids) obtained from plants play important roles in industrial applications as lubricants, protective coatings, plastics, inks, cosmetics, etc. The hundreds of potential industrial uses of mFAs have led to considerable interest in exploring their production in transgenic crop plants. mFAs are produced by a limited number of species, and the transfer of genes encoding mFA-producing enzymes from source plants to heterologous hosts has generally resulted in only modest accumulation, usually less than 20% of the desired mFA in transgenic seed (Napier, 2007) compared with levels found in the natural source. For example, ricinoleic acid accounts for more than 90% of the fatty acid of castor bean (Ricinus communis) seeds, and tung (Aleuites fordii) seeds accumulate more than 80% α-eleostearic acid (Thelen and Ohlrogge, 2002; Drexler et al., 2003). In order to elevate the content of mFAs in the engineered plants to that found in the native plant, it is necessary to (1) optimize the synthesis of mFA (Mekhedov et al., 2001), (2) minimize its degradation (Eccleston and Ohlrogge, 1998), and (3) optimize its incorporation into triacylglycerol (TAG; Bafor et al., 1990; Bates and Browse, 2011; van Erp et al., 2011).Cyclic fatty acids (CFAs) are desirable for numerous industrial applications. The strained bond angles of the carbocyclic ring contribute to their unique chemistry and physical properties, and hydrogenation of CFAs results in ring opening to produce methyl-branched fatty acids. Branched chain fatty acids are ideally suited for the oleochemical industry as feedstocks for the production of lubricants, plastics, paints, dyes, and coatings (Carlsson et al., 2011). Cyclopropane fatty acids (CPAs) have been found in certain gymnosperms, Malvales, Litchi spp., and other Sapindales species. They accumulate to as much as 40% in seeds of Litchi chinensis (Vickery, 1980; Gaydou et al., 1993). Sterculia foetida accumulates the desaturated CFA (i.e. cyclopropene fatty acid) to more than 60% of its seed oil (Bohannon and Kleiman, 1978; Pasha and Ahmad, 1992). The first step in its synthesis is the formation of the CPA by the cyclopropane synthase (CPS) enzyme, which transfers a methyl group to C9 of the oleoyl-phospholipid followed by cyclization to form the cyclopropane ring (Grogan and Cronan, 1997; Bao et al., 2002, 2003). None of the known natural sources of CPA are suitable for its commercial production. Therefore, it would be desirable to create an oilseed crop plant that accumulates high levels of CPA by heterologously expressing CPS in seeds. However, to date, heterologous expression of plant cyclopropane synthase genes has led to only approximately 1.0% CPA in the transgenic seeds (Yu et al., 2011).Two pathways for the biosynthesis of TAG exist in plants (Bates and Browse, 2012; Fig. 1). The de novo biosynthesis from glycerol-3-phosphate and acyl-CoA occurs via the Kennedy pathway and includes three acyltransferases: glycerol-2-phosphate acyltransferase, acyl-CoA:lysophosphatidic acid acyltransferase (LPAT), and acyl-CoA:diacylglycerol acyltransferase (DGAT; Kennedy, 1961). Alternatively, acyl-CoAs can be redirected from phosphatidylcholine (PC) via the action of a phospholipase C, choline phosphotransferase, phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT; Hu et al., 2012; Lu et al., 2009), or phospholipid:diacylglycerol acyltransferase (PDAT; Dahlqvist et al., 2000). An acyl group can be released from PC to generate lysophosphatidylcholine (LPC) by the back reaction of acyl-CoA:LPC acyltransferase (Stymne and Stobart, 1984; Wang et al., 2012) or a phospholipase A/acyl-CoA synthase (Chen et al., 2011).Open in a separate windowFigure 1.Schematic representation of the plant TAG biosynthesis network. Acyl editing can provide PC-modified fatty acids for de novo diacylglycerol/TAG synthesis. ACS, acyl-CoA synthase; CPT, CDP-choline:diacylglycerol choline phosphotransferase; G3P, glycerol-3-phosphate; GPAT, acyl-CoA:glycerol-3-phosphate acyltransferase; LPC acyltransferase, acyl-CoA:LPC acyltransferase; mFAS, modified fatty acid synthase (in this work, mFAS is CPS); PAP, phosphatidic acid phosphatase; PLA, phospholipase A; PLC, phospholipase C.LPAT is a pivotal enzyme controlling the metabolic flow of lysophosphatidic acid (LPA) into different phosphatidic acids (PAs) in diverse tissues. Membrane-associated LPAT activities, identified in bacteria, yeast, plant, and animal cells, catalyze the transfer of acyl groups from acyl-CoA to LPA to synthesize PA. In plants and other organisms, LPAT activities have been identified in the endoplasmic reticulum (Kim et al., 2005), plasma membrane (Bursten et al., 1991), and mitochondria (Zborowski and Wojtczak, 1969). In higher plants, endoplasmic reticulum-localized LPAT plays an essential role transferring fatty acid from CoA esters to the sn-2 position of LPA in the synthesis of PA, a key intermediate in the biosynthesis of membrane phospholipids and storage lipids in developing seeds (Maisonneuve et al., 2010). LPAT from developing seeds of flax (Linum usitatissimum), rape (Brassica napus), and castor bean preferentially incorporate oleoyl-CoA, weakly incorporate cyclopropane acyl-CoA, and were unable to incorporate methyl-branched acyl-CoA when presented with an equimolar mix of these potential substrates (Nlandu Mputu et al., 2009). Thus, LPAT activity from agronomic plants constitutes a potential bottleneck for the incorporation of branched chain acyl-CoA into PA. In this work, we investigate the utility of an LPAT from a cyclopropanoid-syntheizing plant, S. foetida, with respect to its ability to enhance CPA accumulation. In our efforts to enhance CPA accumulation in transgenic plants, we screened CPS genes from diverse sources and identified Escherichia coli CPS (EcCPS) as an effective enzyme for the production of CPA in plants. However, EcCPS is reported to prefer the sn-2 position of E. coli phospholipid (Hildebrand and Law, 1964), and the data presented here show that its expression primarily leads to the accumulation of CPA at the stereospecific numbering (sn)-1 position. Moreover, coexpression of S. foetida lysophosphatidic acid acyltransferase (SfLPAT) results in the incorporation of CPA at the sn-2 position of LPA. Thus, coexpression of EcCPS and SfLPAT enables a cycle that enriches the accumulation of CPA at both sn-1 and sn-2 positions of PC and increases the accumulation of CPA. This work underscores the utility of coexpressing an acyltransferase from mFA-accumulating species with mFA-synthesizing enzymes to help mitigate bottlenecks in mFA TAG synthesis.  相似文献   

5.
Plasmodesmata (Pd) are membranous channels that serve as a major conduit for cell-to-cell communication in plants. The Pd-associated β-1,3-glucanase (BG_pap) and CALLOSE BINDING PROTEIN1 (PDCB1) were identified as key regulators of Pd conductivity. Both are predicted glycosylphosphatidylinositol-anchored proteins (GPI-APs) carrying a conserved GPI modification signal. However, the subcellular targeting mechanism of these proteins is unknown, particularly in the context of other GPI-APs not associated with Pd. Here, we conducted a comparative analysis of the subcellular targeting of the two Pd-resident and two unrelated non-Pd GPI-APs in Arabidopsis (Arabidopsis thaliana). We show that GPI modification is necessary and sufficient for delivering both BG_pap and PDCB1 to Pd. Moreover, the GPI modification signal from both Pd- and non-Pd GPI-APs is able to target a reporter protein to Pd, likely to plasma membrane microdomains enriched at Pd. As such, the GPI modification serves as a primary Pd sorting signal in plant cells. Interestingly, the ectodomain, a region that carries the functional domain in GPI-APs, in Pd-resident proteins further enhances Pd accumulation. However, in non-Pd GPI-APs, the ectodomain overrides the Pd targeting function of the GPI signal and determines a specific GPI-dependent non-Pd localization of these proteins at the plasma membrane and cell wall. Domain-swap analysis showed that the non-Pd localization is also dominant over the Pd-enhancing function mediated by a Pd ectodomain. In conclusion, our results indicate that segregation between Pd- and non-Pd GPI-APs occurs prior to Pd targeting, providing, to our knowledge, the first evidence of the mechanism of GPI-AP sorting in plants.Plant cells are interconnected with cross-wall membranous channels called plasmodesmata (Pd). Recent studies have shown that the region of the plasma membrane (PM) lining the Pd channel is a specialized membrane microdomain whose lipid and protein composition differs from the rest of the PM (Tilsner et al., 2011, 2016; Bayer et al., 2014; González-Solís et al., 2014; Grison et al., 2015). In a similar manner, the cell wall domain surrounding the Pd channel is specialized and, unlike the rest of the cell wall, is devoid of cellulose, rich in pectin, and contains callose (an insoluble β-1,3-glucan; Zavaliev et al., 2011; Knox and Benitez-Alfonso, 2014). In response to physiological signals, callose can be transiently deposited and degraded at Pd, which provides a mechanism for controlling the Pd aperture in diverse developmental and stress-related processes (Zavaliev et al., 2011). Control of Pd functioning is mediated by proteins that are specifically targeted to Pd. Plasmodesmal proteins localized to the PM domain of Pd can be integral transmembrane proteins, such as Pd-localized proteins (Thomas et al., 2008), the receptor kinase ARABIDOPSIS CRINKLY4 (Stahl et al., 2013), and callose synthases (Vatén et al., 2011). Alternatively, Pd proteins can associate with the membrane through a lipid modification like myristoylation (e.g. remorins; Raffaele et al., 2009) or be attached by a glycosylphosphatidylinositol (GPI) anchor (e.g. Pd-associated β-1,3-glucanases [BG_pap]; Levy et al., 2007; Rinne et al., 2011; Benitez-Alfonso et al., 2013), Pd-associated callose-binding proteins (PDCBs; Simpson et al., 2009), and LYSIN MOTIF DOMAIN-CONTAINING PROTEIN2 (LYM2; Faulkner et al., 2013).Among the known Pd proteins involved in Pd-specific callose degradation is BG_pap, a cell wall enzyme carrying a glycosyl hydrolase family 17 (GH17) module as its functional domain (Levy et al., 2007). Another group of proteins controlling callose dynamics at Pd are PDCBs that harbor a callose-binding domain termed carbohydrate-binding module 43 (CBM43), implicated in stabilizing callose at Pd (Simpson et al., 2009). Some β-1,3-glucanases may combine the two callose-modifying activities by harboring both GH17 and CBM43 functional domains, and several such proteins were shown to localize to Pd (Rinne et al., 2011; Benitez-Alfonso et al., 2013; Gaudioso-Pedraza and Benitez-Alfonso, 2014). A distinct feature of BG_pap and PDCBs is that both are predicted glycosylphosphatidylinositol-anchored proteins (GPI-APs). The GPI anchor is a form of posttranslational modification common to many cell surface proteins in all eukaryotes. GPI-APs are covalently attached to the outer leaflet of the PM through the GPI anchor. The basic structure of the anchor consists of ethanolamine phosphate, followed by a glycan chain of three Man residues and glucosamine, followed by phosphatidylinositol lipid moiety (EtNP-6Manα1-2Manα1-6Manα1-4GlcNα1-6myoinositol-1-P-lipid; Ferguson et al., 2009). All predicted GPI-APs carry an N-terminal secretion signal peptide (SP) similar to other secreted proteins. Distinctly, GPI-APs also carry a structurally conserved 25- to 30-residue C-terminal GPI attachment signal, which typically begins with a small amino acid (e.g. Ala, Asn, Asp, Cys, Gly, or Ser) termed omega, followed by a spacer region of five to 10 polar residues, and ending with a transmembrane segment of 15 to 20 hydrophobic residues (Ferguson et al., 2009). The entire region between the N-terminal and the C-terminal signals of a GPI-AP is termed the ectodomain and carries the protein’s functional domain(s). The GPI modification process takes place in the lumenal face of the endoplasmic reticulum (ER) in a cotranslational manner. Upon translocation into the ER, a GPI-AP is stabilized in the ER membrane by its C-terminal signal, which is concurrently cleaved after the omega amino acid, and a preassembled GPI anchor is covalently attached to the C terminus of the omega amino acid. After attachment to a protein, the GPI anchor undergoes a series of modifications (remodeling), both at the glucan chain and at the lipid moiety. Such remodeling is crucial for the sorting of GPI-APs in the secretory pathway and the subsequent lateral heterogeneity at the PM (Kinoshita, 2015). In particular, the addition of saturated fatty acid chains to the lipid moiety of the anchor leads to the enriched accumulation of GPI-APs in the PM microdomains, also termed lipid rafts (Muñiz and Zurzolo, 2014). In Arabidopsis (Arabidopsis thaliana), GPI modification has been predicted for 210 proteins of diverse functions at the PM or the cell wall or both (Borner et al., 2002). Despite extensive research on the GPI modification pathway and the function of GPI-APs in mammalian and yeast cells, such knowledge in plant systems is scarce. In particular, despite an emerging role of GPI-APs in the regulation of the cell wall domain of Pd, their subcellular targeting and compartmentalization mechanism have not been studied. In addition, it is not known how the targeting mechanism of Pd-resident GPI-APs is different from that of other classes of GPI-APs, which are not localized to Pd.In this study, we investigated the subcellular targeting mechanism of Pd-associated callose-modifying GPI-APs, BG_pap and PDCB1, and compared it with that of two unrelated non-Pd GPI-APs, ARABINOGALACTAN PROTEIN4 (AGP4) and LIPID TRANSFER PROTEIN1 (LTPG1). Using sequential fluorescent labeling of protein domains, we found that the C-terminal GPI modification signal present in both Pd- and non-Pd GPI-APs can function as a primary signal in targeting proteins to the Pd-enriched PM domain. Moreover, we show that while the GPI signal is sufficient for Pd targeting, the ectodomains in BG_pap and PDCB1 further enhance their accumulation at Pd. In contrast, the ectodomains in non-Pd GPI-APs mediate exclusion of the proteins from the Pd-enriched targeting pathway. The Pd exclusion effect was found to be dominant over the Pd-targeting function of the GPI signal and the Pd-enhancing function of the Pd ectodomain, and it possibly occurs prior to PM localization. Our findings thus uncover a novel Pd-targeting signal and provide, to our knowledge, the first evidence of the cellular mechanism that regulates the sorting of GPI-APs in plants.  相似文献   

6.
7.
The plant root is the first organ to encounter salinity stress, but the effect of salinity on root system architecture (RSA) remains elusive. Both the reduction in main root (MR) elongation and the redistribution of the root mass between MRs and lateral roots (LRs) are likely to play crucial roles in water extraction efficiency and ion exclusion. To establish which RSA parameters are responsive to salt stress, we performed a detailed time course experiment in which Arabidopsis (Arabidopsis thaliana) seedlings were grown on agar plates under different salt stress conditions. We captured RSA dynamics with quadratic growth functions (root-fit) and summarized the salt-induced differences in RSA dynamics in three growth parameters: MR elongation, average LR elongation, and increase in number of LRs. In the ecotype Columbia-0 accession of Arabidopsis, salt stress affected MR elongation more severely than LR elongation and an increase in LRs, leading to a significantly altered RSA. By quantifying RSA dynamics of 31 different Arabidopsis accessions in control and mild salt stress conditions, different strategies for regulation of MR and LR meristems and root branching were revealed. Different RSA strategies partially correlated with natural variation in abscisic acid sensitivity and different Na+/K+ ratios in shoots of seedlings grown under mild salt stress. Applying root-fit to describe the dynamics of RSA allowed us to uncover the natural diversity in root morphology and cluster it into four response types that otherwise would have been overlooked.Salt stress is known to affect plant growth and productivity as a result of its osmotic and ionic stress components. Osmotic stress imposed by salinity is thought to act in the early stages of the response, by reducing cell expansion in growing tissues and causing stomatal closure to minimize water loss. The build-up of ions in photosynthetic tissues leads to toxicity in the later stages of salinity stress and can be reduced by limiting sodium transport into the shoot tissue and compartmentalization of sodium ions into the root stele and vacuoles (Munns and Tester, 2008). The effect of salt stress on plant development was studied in terms of ion accumulation, plant survival, and signaling (Munns et al., 2012; Hasegawa, 2013; Pierik and Testerink, 2014). Most studies focus on traits in the aboveground tissues, because minimizing salt accumulation in leaf tissue is crucial for plant survival and its productivity. This approach has led to the discovery of many genes underlying salinity tolerance (Munns and Tester, 2008; Munns et al., 2012; Hasegawa, 2013; Maathuis, 2014). Another way to estimate salinity stress tolerance is by studying the rate of main root (MR) elongation of seedlings transferred to medium supplemented with high salt concentration. This is how Salt Overly Sensitive mutants were identified, being a classical example of genes involved in salt stress signaling and tolerance (Hasegawa, 2013; Maathuis, 2014). The success of this approach is to be explained by the important role that the root plays in salinity tolerance. Roots not only provide anchorage and ensure water and nutrient uptake, but also act as a sensory system, integrating changes in nutrient availability, water content, and salinity to adjust root morphology to exploit available resources to the maximum capacity (Galvan-Ampudia et al., 2013; Gruber et al., 2013). Understanding the significance of environmental modifications of root system architecture (RSA) for plant productivity is one of the major challenges of modern agriculture (de Dorlodot et al., 2007; Den Herder et al., 2010; Pierik and Testerink, 2014).The RSA of dicotyledonous plants consists of an embryonically derived MR and lateral roots (LRs) that originate from xylem pole pericycle cells of the MR, or from LRs in the case of higher-order LRs. Root growth and branching is mainly guided through the antagonistic action of two plant hormones: auxin and cytokinins (Petricka et al., 2012). Under environmental stress conditions, the synthesis of abscisic acid (ABA), ethylene, and brassinosteroids is known to be induced and to modulate the growth of MRs and LRs (Achard et al., 2006; Osmont et al., 2007; Achard and Genschik, 2009; Duan et al., 2013; Geng et al., 2013). In general, lower concentrations of salt were observed to slightly induce MR and LR elongation, whereas higher concentrations resulted in decreased growth of both MRs and LRs (Wang et al., 2009; Zolla et al., 2010). The reduction of growth is a result of the inhibition of cell cycle progression and a reduction in root apical meristem size (West et al., 2004). However, conflicting results were presented for the effect of salinity on lateral root density (LRD; Wang et al., 2009; Zolla et al., 2010; Galvan-Ampudia and Testerink, 2011). Some studies suggest that mild salinity enhances LR initiation or emergence events, thereby affecting patterning, whereas other studies imply that salinity arrests LR development. The origin of those contradictory observations could be attributable to studying LR initiation and density at single time points, rather than observing the dynamics of LR development, because LR formation changes as a function of root growth rate (De Smet et al., 2012). The dynamics of LR growth and development were characterized previously for the MR region formed before the salt stress exposure, identifying the importance of ABA in early growth arrest of postemerged LRs in response to salt stress (Duan et al., 2013). The effect of salt on LR emergence and initiation was found to differ for MR regions formed prior and subsequent to salinity exposure (Duan et al., 2013), consistent with LR patterning being determined at the root tip (Moreno-Risueno et al., 2010). Yet the effect of salt stress on the reprogramming of the entire RSA on a longer timescale remains elusive.Natural variation in Arabidopsis (Arabidopsis thaliana) is a great source for dissecting the genetic components underlying phenotypic diversity (Trontin et al., 2011; Weigel, 2012). Genes underlying phenotypic plasticity of RSA to environmental stimuli were also found to have high allelic variation leading to differences in root development between different Arabidopsis accessions (Rosas et al., 2013). Supposedly, genes responsible for phenotypic plasticity of the root morphology to different environmental conditions are under strong selection for adaptation to local environments. Various populations of Arabidopsis accessions were used to study natural variation in ion accumulation and salinity tolerance (Rus et al., 2006; Jha et al., 2010; Katori et al., 2010; Roy et al., 2013). In addition, a number of studies focusing on the natural variation in RSA have been published, identifying quantitative trait loci and allelic variation for genes involved in RSA development under control conditions (Mouchel et al., 2004; Meijón et al., 2014) and nutrient-deficient conditions (Chevalier et al., 2003; Gujas et al., 2012; Gifford et al., 2013; Kellermeier et al., 2013; Rosas et al., 2013). Exploring natural variation not only expands the knowledge of genes and molecular mechanisms underlying biological processes, but also provides insight on how plants adapt to challenging environmental conditions (Weigel, 2012) and whether the mechanisms are evolutionarily conserved. The early growth arrest of newly emerged LRs upon exposure to salt stress was observed to be conserved among the most commonly used Arabidopsis accessions Columbia-0 (Col-0), Landsberg erecta, and Wassilewskija (Ws; Duan et al., 2013). By studying salt stress responses of the entire RSA and a wider natural variation in root responses to stress, one could identify new morphological traits that are under environmental selection and possibly contribute to stress tolerance.In this work, we not only identify the RSA components that are responsive to salt stress, but we also describe the natural variation in dynamics of salt-induced changes leading to redistribution of root mass and different root morphology. The growth dynamics of MRs and LRs under different salt stress conditions were described by fitting a set of quadratic growth functions (root-fit) to individual RSA components. Studying salt-induced changes in RSA dynamics of 31 Arabidopsis accessions revealed four major strategies conserved among the accessions. Those four strategies were due to differences in salt stress sensitivity of individual RSA components (i.e. growth rates of MRs and LRs, and increases in the number of emerged LRs). This diversity in root morphology responses caused by salt stress was observed to be partially associated with differences in ABA, but not ethylene sensitivity. In addition, we observed that a number of accessions exhibiting a relatively strong inhibition of LR elongation showed a smaller increase in the Na+/K+ ratio in shoot tissue after exposure to salt stress. Our results imply that different RSA strategies identified in this study reflect diverse adaptations to different soil conditions and thus might contribute to efficient water extraction and ion compartmentalization in their native environments.  相似文献   

8.
Most proteins produced in the endoplasmic reticulum (ER) of eukaryotic cells fold via disulfide formation (oxidative folding). Oxidative folding is catalyzed by protein disulfide isomerase (PDI) and PDI-related ER protein thiol disulfide oxidoreductases (ER oxidoreductases). In yeast and mammals, ER oxidoreductin-1s (Ero1s) supply oxidizing equivalent to the active centers of PDI. In this study, we expressed recombinant soybean Ero1 (GmERO1a) and found that GmERO1a oxidized multiple soybean ER oxidoreductases, in contrast to mammalian Ero1s having a high specificity for PDI. One of these ER oxidoreductases, GmPDIM, associated in vivo and in vitro with GmPDIL-2, was unable to be oxidized by GmERO1a. We therefore pursued the possible cooperative oxidative folding by GmPDIM, GmERO1a, and GmPDIL-2 in vitro and found that GmPDIL-2 synergistically accelerated oxidative refolding. In this process, GmERO1a preferentially oxidized the active center in the a′ domain among the a, a′, and b domains of GmPDIM. A disulfide bond introduced into the active center of the a′ domain of GmPDIM was shown to be transferred to the active center of the a domain of GmPDIM and the a domain of GmPDIM directly oxidized the active centers of both the a or a′ domain of GmPDIL-2. Therefore, we propose that the relay of an oxidizing equivalent from one ER oxidoreductase to another may play an essential role in cooperative oxidative folding by multiple ER oxidoreductases in plants.In eukaryotes, many secretory and membrane proteins fold via disulfide bond formation in the endoplasmic reticulum (ER). Seed storage proteins of major crops, such as wheat, corn, rice, and beans, which are important protein sources for humans and domestic animals, are synthesized in the ER of the endosperm or cotyledon. A number of seed storage proteins fold by the formation of intramolecular disulfide bonds (oxidative folding) and are transported to and accumulate in protein bodies (Kermode and Bewley, 1999; Jolliffe et al., 2005). In contrast to normally folded proteins, misfolded and unfolded proteins are retained in the ER and degraded by an ER-associated degradation or vacuolar system (Smith et al., 2011; Pu and Bassham, 2013). Therefore, quick and efficient oxidative folding of nascent seed storage proteins is needed for their accumulation in protein bodies.During this process, protein disulfide isomerase (PDI; EC 5.3.4.1) and other ER protein thiol disulfide oxidoreductases (ER oxidoreductases) are thought to catalyze the formation and isomerization of disulfide bonds in nascent proteins (Hatahet and Ruddock, 2009; Feige and Hendershot, 2011; Lu and Holmgren, 2014). After phylogenetic analysis of the Arabidopsis genome, 10 classes of ER oxidoreductases (classes I–X) were identified (Houston et al., 2005). Among them, class I ER oxidoreductase, a plant PDI ortholog, has been studied in a wide variety of plants. Class I ER oxidoreductases have two catalytically active domains a and a′, containing active centers composed of Cys-Gly-His-Cys and two catalytically inactive domains b and b′. An Arabidopsis ortholog of class I ER oxidoreductases is required for proper seed development and regulates the timing of programmed cell death by chaperoning and inhibiting Cys proteases (Andème Ondzighi et al., 2008). OaPDI, a PDI from Oldenlandia affinis, a coffee family (Rubiaceae) plant, is involved in the folding of knotted circular proteins (Gruber et al., 2007). The rice ortholog (PDIL1-1) was suggested to be involved in the maturation of the major seed storage protein glutelin (Takemoto et al., 2002). Furthermore, rice PDIL1-1 plays a role in regulatory activities for various proteins that are essential for the synthesis of grain components as determined by analysis of a T-DNA insertion mutant (Satoh-Cruz et al., 2010).The oxidative refolding ability of class I ER oxidoreductases was confirmed in recombinant soybean (GmPDIL-1) and wheat proteins produced by an Escherichia coli expression system established from cDNAs (Kamauchi et al., 2008; Kimura et al., 2015).Class II and III ER oxidoreductases have an a–b–b′–a′ domain structure. Class II ER oxidoreductases have an acidic amino acid-rich sequence in the N-terminal region ahead of the a domain. Recombinant soybean (GmPDIL-2) and wheat class II ER oxidoreductases have oxidative refolding activities similar to that of class I (Kamauchi et al., 2008; Kimura et al., 2015). Class III ER oxidoreductases contain the nonclassical redox-center Cys-X-X-Ser/Cys motifs, as opposed to the more traditional CGHC sequence, in the a and a′ domains. Recombinant soybean (GmPDIL-3) and wheat proteins lack oxidative refolding activity in vitro (Iwasaki et al., 2009; Kimura et al., 2015). Class IV ER oxidoreductases are unique to plants and have an a–a′–ERp29 domain structure, which is homologous to the C-terminal domain of mammalian ERp29 (Demmer et al., 1997).Recombinant soybean class IV ER oxidoreductases (GmPDIS-1 and GmPDIS-2) and wheat class IV ER oxidoreductase possess an oxidative refolding activity that is weaker than that of classes I and II (Wadahama et al., 2007; Kimura et al., 2015). Class V ER oxidoreductases are plant orthologs of mammalian P5 and have an a–a′–b domain structure. A rice class V ER oxidoreductase, consisting of PDIL2 and PDIL3, plays an important role in the accumulation of the seed storage protein Cys-rich 10-kD prolamin (crP10; Onda et al., 2011). Recombinant soybean class V ER oxidoreductase, GmPDIM and wheat class V ER oxidoreductase possess an oxidative refolding activity similar to that of class IV (Wadahama et al., 2008; Kimura et al., 2015). In the soybean, GmPDIL-1, GmPDIL-2, GmPDIM, GmPDIS-1, and GmPDIS-2 were found to associate transiently with a seed storage precursor protein, proglycinin, in the ER of the cotyledon by coimmunoprecipitation experiments, suggesting that multiple ER oxidoreductases are involved in the folding of the nascent proglycinin.The disulfide bond in the active center of ER oxidoreductases is reduced as a result of catalyzing disulfide bond formation in an unfolded protein. The reduced active center of PDI was discovered to be oxidized again by ER oxidoreductin-1 (Ero1p) in yeast (Frand and Kaiser, 1998; Pollard et al., 1998). Ero1p orthologs are present universally in eukaryotes. Yeast and flies have a single copy of the ERO1 gene, which is essential for survival (Frand and Kaiser, 1998; Pollard et al., 1998; Tien et al., 2008). Mammals have two genes encoding Ero1-α (Cabibbo et al., 2000) and Ero1-β (Pagani et al., 2000) that function as major disulfide donors to nascent proteins in the ER, but are not critical for survival (Zito et al., 2010). Domain a of yeast PDI is the most favored substrate of yeast Ero1p (Vitu et al., 2010), whereas a′ of human PDI is specifically oxidized by human Ero1-α (Chambers et al., 2010) and Ero1-β (Wang et al., 2011). Electrons from Cys residues of the active centers of PDI are transferred to oxygen by Ero1 (Tu and Weissman, 2004; Sevier and Kaiser, 2008). The reaction mechanisms of yeast Ero1p and human Ero1s have been intensively investigated; their regulation by PDI has been extensively studied as well (Tavender and Bulleid, 2010; Araki and Inaba, 2012; Benham et al., 2013; Ramming et al., 2015). Only rice Ero1 (OsERO1) has been identified as a plant ortholog of Ero1p (Onda et al., 2009). OsERO1 is necessary for disulfide bond formation in rice endosperm. The formation of native disulfide bonds in the major seed storage protein proglutelin was demonstrated to depend upon OsERO1 by RNAi knockdown experiments. However, no plant protein thiol disulfide oxidoreductases that are oxidized by a plant Ero1 ortholog have been identified to date.In this study, we show that multiple soybean ER oxidoreductases can be activated by a soybean Ero1 ortholog (GmERO1a). In addition, we propose a synergistic mechanism by which GmPDIM and GmPDIL-2 cooperatively fold unfolded proteins using oxidizing equivalents provided by GmERO1 in vitro.  相似文献   

9.
10.
11.
12.
Quantitative proteome analyses suggest that the well-established stain colloidal Coomassie Blue, when used as an infrared dye, may provide sensitive, post-electrophoretic in-gel protein detection that can rival even Sypro Ruby. Considering the central role of two-dimensional gel electrophoresis in top-down proteomic analyses, a more cost effective alternative such as Coomassie Blue could prove an important tool in ongoing refinements of this important analytical technique. To date, no systematic characterization of Coomassie Blue infrared fluorescence detection relative to detection with SR has been reported. Here, seven commercial Coomassie stain reagents and seven stain formulations described in the literature were systematically compared. The selectivity, threshold sensitivity, inter-protein variability, and linear-dynamic range of Coomassie Blue infrared fluorescence detection were assessed in parallel with Sypro Ruby. Notably, several of the Coomassie stain formulations provided infrared fluorescence detection sensitivity to <1 ng of protein in-gel, slightly exceeding the performance of Sypro Ruby. The linear dynamic range of Coomassie Blue infrared fluorescence detection was found to significantly exceed that of Sypro Ruby. However, in two-dimensional gel analyses, because of a blunted fluorescence response, Sypro Ruby was able to detect a few additional protein spots, amounting to 0.6% of the detected proteome. Thus, although both detection methods have their advantages and disadvantages, differences between the two appear to be small. Coomassie Blue infrared fluorescence detection is thus a viable alternative for gel-based proteomics, offering detection comparable to Sypro Ruby, and more reliable quantitative assessments, but at a fraction of the cost.Gel electrophoresis is an accessible, widely applicable and mature protein resolving technology. As the original top-down approach to proteomic analyses, among its many attributes the high resolution achievable by two dimensional gel-electrophoresis (2DE)1 ensures that it remains an effective analytical technology despite the appearance of alternatives. However, in-gel detection remains a limiting factor for gel-based analyses; available technology generally permits the detection and quantification of only relatively abundant proteins (35). Many critical components in normal physiology and also disease may be several orders of magnitude less abundant and thus below the detection threshold of in-gel stains, or indeed most techniques. Pre- and post-fractionation technologies have been developed to address this central issue in proteomics but these are not without limitations (15). Thus improved detection methods for gel-based proteomics continue to be a high priority, and the literature is rich with different in-gel detection methods and innovative improvements (634). This history of iterative refinement presents a wealth of choices when selecting a detection strategy for a gel-based proteomic analysis (35).Perhaps the best known in-gel detection method is the ubiquitous Coomassie Blue (CB) stain; CB has served as a gel stain and protein quantification reagent for over 40 years. Though affordable, robust, easy to use, and compatible with mass spectrometry (MS), CB staining is relatively insensitive. In traditional organic solvent formulations, CB detects ∼ 10 ng of protein in-gel, and some reports suggest poorer sensitivity (27, 29, 36, 37). Sensitivity is hampered by relatively high background staining because of nonspecific retention of dye within the gel matrix (32, 36, 38, 39). The development of colloidal CB (CCB) formulations largely addressed these limitations (12); the concentration of soluble CB was carefully controlled by sequestering the majority of the dye into colloidal particles, mediated by pH, solvent, and the ionic strength of the solution. Minimizing soluble dye concentration and penetration of the gel matrix mitigated background staining, and the introduction of phosphoric acid into the staining reagent enhanced dye-protein interactions (8, 12, 40), contributing to an in-gel staining sensitivity of 5–10 ng protein, with some formulations reportedly yielding sensitivities of 0.1–1 ng (8, 12, 22, 39, 41, 42). Thus CCB achieved higher sensitivity than traditional CB staining, yet maintained all the advantages of the latter, including low cost and compatibility with existing densitometric detection instruments and MS. Although surpassed by newer methods, the practical advantages of CCB ensure that it remains one of the most common gel stains in use.Fluorescent stains have become the routine and sensitive alternative to visible dyes. Among these, the ruthenium-organometallic family of dyes have been widely applied and the most commercially well-known is Sypro Ruby (SR), which is purported to interact noncovalently with primary amines in proteins (15, 18, 19, 43). Chief among the attributes of these dyes is their high sensitivity. In-gel detection limits of < 1 ng for some proteins have been reported for SR (6, 9, 14, 44, 45). Moreover, SR staining has been reported to yield a greater linear dynamic range (LDR), and reduced interprotein variability (IPV) compared with CCB and silver stains (15, 19, 4649). SR is easy to use, fully MS compatible, and relatively forgiving of variations in initial conditions (6, 15). The chief consequence of these advances remains high cost; SR and related stains are notoriously expensive, and beyond the budget of many laboratories. Furthermore, despite some small cost advantage relative to SR, none of the available alternatives has been consistently and quantitatively demonstrated to substantially improve on the performance of SR under practical conditions (9, 50).Notably, there is evidence to suggest that CCB staining is not fundamentally insensitive, but rather that its sensitivity has been limited by traditional densitometric detection (50, 51). When excited in the near IR at ∼650 nm, protein-bound CB in-gel emits light in the range of 700–800 nm. Until recently, the lack of low-cost, widely available and sufficiently sensitive infrared (IR)-capable imaging instruments prevented mainstream adoption of in-gel CB infrared fluorescence detection (IRFD); advances in imaging technology are now making such instruments far more accessible. Initial reports suggested that IRFD of CB-stained gels provided greater sensitivity than traditional densitometric detection (50, 51). Using CB R250, in-gel IRFD was reported to detect as little as 2 ng of protein in-gel, with a LDR of about an order of magnitude (2 to 20 ng, or 10 to 100 ng in separate gels), beyond which the fluorescent response saturated into the μg range (51). Using the G250 dye variant, it was determined that CB-IRFD of 2D gels detected ∼3 times as many proteins as densitometric imaging, and a comparable number of proteins as seen by SR (50). This study also concluded that CB-IRFD yielded a significantly higher signal to background ratio (S/BG) than SR, providing initial evidence that CB-IRFD may be superior to SR in some aspects of stain performance (50).Despite this initial evidence of the viability of CB-IRF as an in-gel protein detection method, a detailed characterization of this technology has not yet been reported. Here a more thorough, quantitative characterization of CB-IRFD is described, establishing its lowest limit of detection (LLD), IPV, and LDR in comparison to SR. Finally a wealth of modifications and enhancements of CCB formulations have been reported (8, 12, 21, 24, 26, 29, 40, 41, 5254), and likewise there are many commercially available CCB stain formulations. To date, none of these formulations have been compared quantitatively in terms of their relative performance when detected using IRF. As a general detection method for gel-based proteomics, CB-IRFD was found to provide comparable or even slightly superior performance to SR according to most criteria, including sensitivity and selectivity (50). Furthermore, in terms of LDR, CB-IRFD showed distinct advantages over SR. However, assessing proteomes resolved by 2DE revealed critical distinctions between CB-IRFD and SR in terms of protein quantification versus threshold detection: neither stain could be considered unequivocally superior to the other by all criteria. Nonetheless, IRFD proved the most sensitive method of detecting CB-stained protein in-gel, enabling high sensitivity detection without the need for expensive reagents or even commercial formulations. Overall, CB-IRFD is a viable alternative to SR and other mainstream fluorescent stains, mitigating the high cost of large-scale gel-based proteomic analyses, making high sensitivity gel-based proteomics accessible to all labs. With improvements to CB formulations and/or image acquisition instruments, the performance of this detection technology may be further enhanced.  相似文献   

13.
Female control of nonrandom mating has never been genetically established, despite being linked to inbreeding depression and sexual selection. In order to map the loci that control female-mediated nonrandom mating, we constructed a new advanced intercross recombinant inbred line (RIL) population derived from a cross between Arabidopsis (Arabidopsis thaliana) accessions Vancouver (Van-0) and Columbia (Col-0) and mapped quantitative trait loci (QTLs) responsible for nonrandom mating and seed yield traits. We genotyped a population of 490 RILs. A subset of these lines was used to construct an expanded map of 1,061.4 centimorgans with an average interval of 6.7 ± 5.3 centimorgans between markers. QTLs were then mapped for female- and male-mediated nonrandom mating and seed yield traits. To map the genetic loci responsible for female-mediated nonrandom mating and seed yield, we performed mixed pollinations with genetically marked Col-0 pollen and Van-0 pollen on RIL pistils. To map the loci responsible for male-mediated nonrandom mating and seed yield, we performed mixed pollinations with genetically marked Col-0 and RIL pollen on Van-0 pistils. Composite interval mapping of these data identified four QTLs that control female-mediated nonrandom mating and five QTLs that control female-mediated seed yield. We also identified four QTLs that control male-mediated nonrandom mating and three QTLs that control male-mediated seed yield. Epistasis analysis indicates that several of these loci interact. To our knowledge, the results of these experiments represent the first time female-mediated nonrandom mating has been genetically defined.The process of pollination offers plants the opportunity to selectively breed. For example, in pollinations that include more than one pollen population, pollen often show differential siring ability. This process is called nonrandom mating. Although pollen may fail in pollinations because they are self pollen in an obligate outcrossing plant or pollen from a different species, we focus our studies on differential siring ability of compatible, conspecific mates (Hogenboom, 1973, 1975; Williams et al., 1999; de Nettancourt, 2001; Husband et al., 2002; Wheeler et al., 2009; Meng et al., 2011; Nasrallah, 2011). Nonrandom mating at this level has received intense interest for its potential to avoid inbreeding depression and its potential to be the result of sexual selection (Charnov, 1979; Mulcahy, 1979; Willson, 1979; Queller, 1983; Stephenson and Bertin, 1983; Willson and Burley, 1983; Marshall and Ellstrand, 1986; Charlesworth and Charlesworth, 1987; Mulcahy and Mulcahy, 1987; Cruzan, 1990; Quesada et al., 1993; Snow, 1994; Paschke et al., 2002; Skogsmyr and Lankinen, 2002; Stephenson et al., 2003; Armbruster and Rogers, 2004; Bernasconi et al., 2004; Lankinen and Armbruster, 2007). Despite a long history of theoretical and experimental attention, very little is known about the underlying genetics that govern the process (Carlson et al., 2011).One challenge in understanding the genetics of nonrandom mating lies in its complexity, potentially involving multiple distinct pathways specific to either female or male tissues. Physiologically, postpollination nonrandom mating may be a result of intrinsic differences in pollen competitive abilities (male-mediated nonrandom mating). A number of experimental strategies have been employed to demonstrate male-mediated control of nonrandom mating. For example, experiments in radish (Raphanus sativus) found that some pollen sire more seeds than others in mixed pollinations across a range of maternal plants, demonstrating consistency of male function (Marshall and Ellstrand, 1986, 1988; Mitchell and Marshall, 1998). More direct measures of male function, such as in vitro and in vivo pollen tube growth rates, verify variation in male function and demonstrable impact on nonrandom mating (Snow and Spira, 1991a, 1991b; Pasonen et al., 1999; Skogsmyr and Lankinen, 1999; Stephenson et al., 2001; Lankinen and Skogsmyr, 2002; Lankinen et al., 2009). Finally, recent work in our laboratory has directly mapped the genetic loci responsible for the control of male-mediated nonrandom mating in Arabidopsis (Arabidopsis thaliana; Carlson et al., 2011).Alternatively, or concurrently, nonrandom mating can be the result of differential interaction between the female tissue and competing pollen populations or seeds (female-mediated nonrandom mating). Establishing the female role in nonrandom mating has been more challenging, as most study designs involve the deposition of pollen from multiple donors and thus include the confounding variable of pollen competition. Despite this challenge, a number of experimental strategies have been devised to explore the role of the female in nonrandom mating. For example, a number of studies demonstrate that maternal identity influences nonrandom mating patterns (Marshall and Ellstrand, 1986, 1988; Snow and Mazer, 1988; Johnston, 1993; Marshall et al., 2000; Carlson et al., 2009, 2013). Studies have also established that manipulation of watering or nutrient regimes of maternal plants changes the patterns and magnitude of nonrandom mating (Marshall and Diggle, 2001; Shaner and Marshall, 2003; Haileselassie et al., 2005; Marshall et al., 2007). These studies and others implicate the identity and condition of the female in the process of nonrandom mating. Despite a long history of research, genetic control of female-mediated nonrandom mating has never been demonstrated, and the identity of the genes involved remains unexplored.In previous work, we developed a system in Arabidopsis to assay nonrandom mating and showed its utility for genetically mapping the loci responsible (Carlson et al., 2009, 2011). Pursuing the genetics of nonrandom mating in a largely selfing plant such as Arabidopsis provides both theoretical and practical advantages. First, outcrossing plants carry higher levels of heterozygosity that produce pollen populations that display different phenotypes because of segregating alleles. This complicates genetic analysis. Also, in outcrossing plants that carry genetic load, reproductive success is context dependent. Pollinations with self pollen or pollen from genetically similar plants often lead to poor reproductive outcomes. For example, in mixed pollinations in generally outcrossing self-compatible plants that include self pollen, self pollen often sire a disproportionally low number of seeds (Bateman, 1956; Weller and Ornduff, 1977; Bowman, 1987; Eckert and Barrett, 1994; Jones, 1994; Hauser and Siegismund, 2000; Teixeira et al., 2009), but other findings have been reported (Sork and Schemske, 1992; Johnston, 1993). Thus, in outcrossing plants, gene variants that influence reproductive success, parental relatedness, and segregating heterozygosity all influence reproductive outcomes. Two of these factors are essentially eliminated by studying plant populations that have historically selfed. As outcrossing populations become increasingly self-fertilizing, they both lose heterozygosity, and their genetic load is purged (Lande and Schemske, 1985; Schemske and Lande, 1985; Charlesworth and Charlesworth, 1987; Lande et al., 1994; Byers and Waller, 1999; Crnokrak and Barrett, 2002). This is the case for Arabidopsis, whose tested populations show relatively low levels of heterozygosity and little evidence for the early-acting inbreeding depression that is indicative of genetic load (Bakker et al., 2006; Bomblies et al., 2010; Platt et al., 2010; Carlson et al., 2013). Thus, this system provides an excellent opportunity to identify and explore the genetic variation in differential reproduction that develops or persists in plant populations unrelated to inbreeding depression.Using this system, we previously identified potential female control of nonrandom mating in mixed pollinations between Vancouver (Van-0) and Columbia (Col-0) accessions of Arabidopsis (Carlson et al., 2009). When Van-0 and genetically marked Col-0 (Col-NPTII) pollen compete on Col-0 pistils, Col-NPTII pollen sire 43% of the progeny, while Van-0 pollen sire 57%. When these pollen compete on Van-0 pistils, Col-NPTII pollen sire 67.5% of the progeny, while Van-0 pollen sire 32.5%. This system offers us, to our knowledge for the first time, the opportunity to genetically define female-mediated nonrandom mating and map the loci responsible.In order to genetically map female control of nonrandom mating, we constructed a new advanced intercross recombinant inbred line (RIL) mapping population derived from a cross between Van-0 and Col-0 accessions of Arabidopsis. RILs are powerful tools that allow high-resolution genetic mapping of loci that direct complex traits. Each RIL contains chromosomes that are defined homozygous patchworks of parental DNA, in this case Van-0 and Col-0. By phenotyping these lines, we can statistically associate nonrandom mating and seed yield phenotypes with chromosomal regions. We chose these two accessions because (1) our previous experiments predict clear female control of nonrandom mating and (2) we have previously mapped male-mediated nonrandom mating controls using a Col-4/Landsberg mapping population (a population that does not display female control of nonrandom mating; Carlson et al., 2011). Thus, this new population provides us the opportunity to map loci that control female nonrandom mating and investigate the degree of conservation of loci that affect male-mediated nonrandom mating. We use this new mapping population to perform quantitative trait locus (QTL) mapping and identify multiple loci that direct both female- and male-mediated control of nonrandom mating and seed yield traits.  相似文献   

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15.
A Boolean network is a model used to study the interactions between different genes in genetic regulatory networks. In this paper, we present several algorithms using gene ordering and feedback vertex sets to identify singleton attractors and small attractors in Boolean networks. We analyze the average case time complexities of some of the proposed algorithms. For instance, it is shown that the outdegree-based ordering algorithm for finding singleton attractors works in time for , which is much faster than the naive time algorithm, where is the number of genes and is the maximum indegree. We performed extensive computational experiments on these algorithms, which resulted in good agreement with theoretical results. In contrast, we give a simple and complete proof for showing that finding an attractor with the shortest period is NP-hard.[1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32]  相似文献   

16.
A large portion of the volatile organic compounds emitted by plants are oxygenated to yield reactive carbonyl species, which have a big impact on atmospheric chemistry. Deposition to vegetation driven by the absorption of reactive carbonyl species into plants plays a major role in cleansing the atmosphere, but the mechanisms supporting this absorption have been little examined. Here, we performed model experiments using methacrolein (MACR), one of the major reactive carbonyl species formed from isoprene, and tomato (Solanum lycopersicum) plants. Tomato shoots enclosed in a jar with MACR vapor efficiently absorbed MACR. The absorption efficiency was much higher than expected from the gas/liquid partition coefficient of MACR, indicating that MACR was likely metabolized in leaf tissues. Isobutyraldehyde, isobutyl alcohol, and methallyl alcohol (MAA) were detected in the headspace and inside tomato tissues treated with MACR vapor, suggesting that MACR was enzymatically reduced. Glutathione (GSH) conjugates of MACR (MACR-GSH) and MAA (MAA-GSH) were also detected. MACR-GSH was essentially formed through spontaneous conjugation between endogenous GSH and exogenous MACR, and reduction of MACR-GSH to MAA-GSH was likely catalyzed by an NADPH-dependent enzyme in tomato leaves. Glutathionylation was the metabolic pathway most responsible for the absorption of MACR, but when the amount of MACR exceeded the available GSH, MACR that accumulated reduced photosynthetic capacity. In an experiment simulating the natural environment using gas flow, MACR-GSH and MAA-GSH accumulation accounted for 30% to 40% of the MACR supplied. These results suggest that MACR metabolism, especially spontaneous glutathionylation, is an essential factor supporting MACR absorption from the atmosphere by tomato plants.Plants emit vast amounts of volatile organic chemicals (VOCs) into the atmosphere. The annual emission of VOCs other than methane is estimated to be approximately 1,300 Tg of carbon (Goldstein and Galbally, 2007), with approximately 90% originating from biogenic sources, of which one-third (approximately 500 Tg of carbon/year) is isoprene (Guenther et al., 1995). In the atmosphere, VOCs undergo the chemical processes of photolysis and reaction with hydroxyl and nitrate radicals (Atkinson and Arey, 2003). Isoprene, for example, is converted into a series of isomeric hydroxyl-substituted alkyl peroxyl radicals, which are further converted into methyl vinyl ketone (MVK; but-3-en-2-one) and methacrolein (MACR; 2-methylprop-2-enal; Liu et al., 2013). These VOCs and their oxygenated products (oVOCs) are important components for the production of ozone and aerosols, and thus have a big impact on atmospheric chemistry and even on the climate system (Goldstein and Galbally, 2007). VOCs and oVOCs are removed from the atmosphere through oxidation to carbon monoxide or dioxide, dry or wet deposition, or secondary aerosol formation (Goldstein and Galbally, 2007). Among these, deposition to vegetation plays a major role in the removal of VOCs and oVOCs from the atmosphere (Karl et al., 2010).A significant portion of the deposition to vegetation is attributable to the uptake of VOCs and oVOCs by plants, and a field study showed that MVK and MACR were immediately lost once they entered a leaf through stomata (Karl et al., 2010). Under growth conditions where stomatal conductance is high enough, the partitioning of VOCs between air and leaf water phases in equilibrium and the capacity of the plant to metabolize, translocate, and store VOCs determine their uptake rate (Tani et al., 2013). The immediate loss in leaves observed with MVK and MACR is indicative of efficient enzymatic reactions metabolizing them; however, the details of the metabolism of these oVOCs have been little investigated so far.The absorption and metabolism of several VOCs by plants have been reported. Airborne ent-kaurene was absorbed by Arabidopsis (Arabidopsis thaliana), Japanese cypress (Chamaecyparis obtusa), and Japanese cedar (Cryptomeria japonica) plants and converted into GAs (Otsuka et al., 2004). Arabidopsis absorbed (Z)-3-hexenal and converted it into (Z)-3-hexen-1-ol or further into (Z)-3-hexen-1-yl acetate using NADPH and acetyl-CoA, probably inside the plant tissues (Matsui et al., 2012). Nicotiana attenuata plants absorbed dimethyl disulfide formed by rhizobacteria (Meldau et al., 2013). The sulfur atom derived from volatile dimethyl disulfide was assimilated into plant proteins. Karl et al. (2010) assumed that aldehyde dehydrogenase, which is involved in detoxification that limits aldehyde accumulation and oxidative stress (Kirch et al., 2004), is involved in the uptake of oVOCs containing an aldehyde moiety; however, they did not provide direct evidence supporting their assumption.Conjugation of VOCs and oVOCs with sugar or glutathione (GSH) is another way to metabolize them. (Z)-3-Hexen-1-ol in the vapor phase was taken up by tomato (Solanum lycopersicum) plants and converted into its glycoside (Sugimoto et al., 2014). (E)-2-Hexenal reacts with GSH spontaneously and/or via glutathione S-transferase (GST) to form hexanal-GSH, which is subsequently reduced to hexanol-GSH (Davoine et al., 2006), although it is uncertain whether airborne (E)-2-hexenal is converted into its corresponding GSH adduct. Glutathionylation of (E)-2-hexenal is common and has been confirmed in grapevine (Vitis vinifera) and passion fruit (Passiflora edulis; Kobayashi et al., 2011; Fedrizzi et al., 2012). The catabolites formed from the GSH adduct in these crops are precursors for important flavor components.Although it is clear that oVOCs are absorbed by vegetation and that their efficient uptake is probably supported by metabolism in plant tissues, the metabolic fates of oVOCs taken up from the vapor phase into plants have been little studied. Here, we performed a series of model experiments using tomato seedlings and MACR to dissect the fates of oVOCs once they entered into plant tissues. To clearly see absorption of MACR and its fates in plant tissues, a model experiment under enclosed conditions with a high concentration of MACR was first carried out. Subsequently, an airflow system with a realistically low concentration of MACR was used. Tomato plants efficiently absorbed MACR. Reduction of the carbonyl moiety and the double bond conjugated to the carbonyl and conjugation with GSH were the major methods of metabolism of exogenous MACR. The metabolism seemed to be involved in the detoxification of reactive carbonyl species, which, in turn, accounted for the oVOC deposition to vegetation.  相似文献   

17.
Human cytomegalovirus (HCMV) is a widely circulating pathogen that causes severe disease in immunocompromised patients and infected fetuses. By immortalizing memory B cells from HCMV-immune donors, we isolated a panel of human monoclonal antibodies that neutralized at extremely low concentrations (90% inhibitory concentration [IC90] values ranging from 5 to 200 pM) HCMV infection of endothelial, epithelial, and myeloid cells. With the single exception of an antibody that bound to a conserved epitope in the UL128 gene product, all other antibodies bound to conformational epitopes that required expression of two or more proteins of the gH/gL/UL128-131A complex. Antibodies against gB, gH, or gM/gN were also isolated and, albeit less potent, were able to neutralize infection of both endothelial-epithelial cells and fibroblasts. This study describes unusually potent neutralizing antibodies against HCMV that might be used for passive immunotherapy and identifies, through the use of such antibodies, novel antigenic targets in HCMV for the design of immunogens capable of eliciting previously unknown neutralizing antibody responses.Human cytomegalovirus (HCMV) is a member of the herpesvirus family which is widely distributed in the human population and can cause severe disease in immunocompromised patients and upon infection of the fetus. HCMV infection causes clinical disease in 75% of patients in the first year after transplantation (58), while primary maternal infection is a major cause of congenital birth defects including hearing loss and mental retardation (5, 33, 45). Because of the danger posed by this virus, development of an effective vaccine is considered of highest priority (51).HCMV infection requires initial interaction with the cell surface through binding to heparan sulfate proteoglycans (8) and possibly other surface receptors (12, 23, 64, 65). The virus displays a broad host cell range (24, 53), being able to infect several cell types such as endothelial cells, epithelial cells (including retinal cells), smooth muscle cells, fibroblasts, leukocytes, and dendritic cells (21, 37, 44, 54). Endothelial cell tropism has been regarded as a potential virulence factor that might influence the clinical course of infection (16, 55), whereas infection of leukocytes has been considered a mechanism of viral spread (17, 43, 44). Extensive propagation of HCMV laboratory strains in fibroblasts results in deletions or mutations of genes in the UL131A-128 locus (1, 18, 21, 36, 62, 63), which are associated with the loss of the ability to infect endothelial cells, epithelial cells, and leukocytes (15, 43, 55, 61). Consistent with this notion, mouse monoclonal antibodies (MAbs) to UL128 or UL130 block infection of epithelial and endothelial cells but not of fibroblasts (63). Recently, it has been shown that UL128, UL130, and UL131A assemble with gH and gL to form a five-protein complex (thereafter designated gH/gL/UL128-131A) that is an alternative to the previously described gCIII complex made of gH, gL, and gO (22, 28, 48, 63).In immunocompetent individuals T-cell and antibody responses efficiently control HCMV infection and reduce pathological consequences of maternal-fetal transmission (13, 67), although this is usually not sufficient to eradicate the virus. Albeit with controversial results, HCMV immunoglobulins (Igs) have been administered to transplant patients in association with immunosuppressive treatments for prophylaxis of HCMV disease (56, 57), and a recent report suggests that they may be effective in controlling congenital infection and preventing disease in newborns (32). These products are plasma derivatives with relatively low potency in vitro (46) and have to be administered by intravenous infusion at very high doses in order to deliver sufficient amounts of neutralizing antibodies (4, 9, 32, 56, 57, 66).The whole spectrum of antigens targeted by HCMV-neutralizing antibodies remains poorly characterized. Using specific immunoabsorption to recombinant antigens and neutralization assays using fibroblasts as model target cells, it was estimated that 40 to 70% of the serum neutralizing activity is directed against gB (6). Other studies described human neutralizing antibodies specific for gB, gH, or gM/gN viral glycoproteins (6, 14, 26, 29, 34, 41, 52, 60). Remarkably, we have recently shown that human sera exhibit a more-than-100-fold-higher potency in neutralizing infection of endothelial cells than infection of fibroblasts (20). Similarly, CMV hyperimmunoglobulins have on average 48-fold-higher neutralizing activities against epithelial cell entry than against fibroblast entry (10). However, epitopes that are targeted by the antibodies that comprise epithelial or endothelial cell-specific neutralizing activity of human immune sera remain unknown.In this study we report the isolation of a large panel of human monoclonal antibodies with extraordinarily high potency in neutralizing HCMV infection of endothelial and epithelial cells and myeloid cells. With the exception of a single antibody that recognized a conserved epitope of UL128, all other antibodies recognized conformational epitopes that required expression of two or more proteins of the gH/gL/UL128-131A complex.  相似文献   

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Mathematical tools developed in the context of Shannon information theory were used to analyze the meaning of the BLOSUM score, which was split into three components termed as the BLOSUM spectrum (or BLOSpectrum). These relate respectively to the sequence convergence (the stochastic similarity of the two protein sequences), to the background frequency divergence (typicality of the amino acid probability distribution in each sequence), and to the target frequency divergence (compliance of the amino acid variations between the two sequences to the protein model implicit in the BLOCKS database). This treatment sharpens the protein sequence comparison, providing a rationale for the biological significance of the obtained score, and helps to identify weakly related sequences. Moreover, the BLOSpectrum can guide the choice of the most appropriate scoring matrix, tailoring it to the evolutionary divergence associated with the two sequences, or indicate if a compositionally adjusted matrix could perform better.[1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29]  相似文献   

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