菹草（Potamogeton crispus）对水中氮、磷的吸收及若干影响因素

菹草（Ｐｏｔａｍｏｇｅｔｏｎ ｃｒｉｓｐｕｓ）对水中氮,磷的吸收与ＰＨ,光照,水温,根／茎生物量比及底泥间隙水与上覆水中营养盐浓度比有关。在ＰＨ为８．０－９．５,水温为１９－２８℃的实验条件下,水中ＮＨ４－Ｎ浓度低于０．３５ｍｇ．１＾－１左右时,菹草茎,叶优先吸收ＮＯ３－Ｎ;水中ＮＨ４－Ｎ浓度大于０．３５ｍｇ．１＾－１左右时,菹草测优先吸收ＮＨ４－Ｎ这一选择吸收与ＮＨ４－Ｎ／ＮＯ３－Ｎ比值无关,强     

盐碱池塘水生大型植物的研究

于１９９８年４月至１９９８年９月对高青盐碱池塘的水生大型植物种类组成,生产力及形影响因子进行了野外调查和试验研究,结果表明高青盐碱池塘共有水生植物１２种。常见的优势种有沉水植物：菹草、角果藻、金鱼藻、;挺水植物、芦苇、荆三棱、莲和香蒲漂浮植物;浮萍,以及大型藻类布氏轮藻。种类少而优势种突出是盐碱池塘大型植物的显特点,有水草池塘与无或水少草池塘相比,透明度大、ＰＨ值高、浮游植物生物量和叶绿素a含量低、营养盐含量也低。在表光照度７２００lx、水温２８－３０℃的条件下,菹草、角果藻和轮藻的毛产量分别为１．７０、１．５６和１．５０mgO2.L^-1.h^-1.g^-1。除光照、水温、ＰＨ等因素外,含盐量和碱度对大型植物的初级生产力具有重要影响,菹草和角果藻的生产力最高时的含直均为３g.L^-1,前适宜的碱度为６．６９mmol.L^-1,而后则为３．８２mmol.L^-1。菹草、角果藻、金鱼藻和浮萍对ＮＨ４－Ｎ的吸收速率相近,而对ＰＯ４－Ｐ的吸收速率则角果藻＞菹草＞浮萍＞金鱼藻。后对盐水水体大型植物特点、菹草生产力及其影响因素,如何防止养鱼池大型植物危害及大型植物与浮游植物的关系进行了讨论。     

黑曲霉生产β-葡萄糖苷酶发酵条件的研究产

经多项式回归分析,研究了不同浓度Ｎ 源、Ｃ 源、无机盐等对酶产量的影响,确定出最佳培养基配方为：麸皮４ ．９ ％ ,（ＮＨ４）２ＳＯ４ ０ ．４ ％ ,ＫＨ２ＰＯ４ ０ ．２９ ％ ,ＣａＣｌ２ ０ ．０５ ％ ,ＭｇＳＯ４·７Ｈ２Ｏ０ ．０４ ％ ,ＦｅＳＯ４·７Ｈ２ Ｏ５ｍｇ·Ｌ－ １ ,ＺｎＣｌ２ １ ．４ｍｇ·Ｌ－ １ ,０ ．２ ％ 油酸钠．并对培养温度、时间、培养基初始ｐＨ、通气量、接种量、接种方式等培养条件进行优化,使黑曲霉生产β葡萄糖苷酶的产量由１７Ｕ·ｍｌ－ １ 增至２１ ．３Ｕ·ｍｌ－ １ ．     

吐尔吉山水库养殖季细菌数量动态及其鱼产力的研究

对吐尔吉山水库养殖季细菌数量测定表明：细菌数波动在０．２６×１０６～１８．６３×１０６ｃｅｌｓ／ｍｌ,高峰期出现在８～１０月。水库有草区细菌数明显高于无草区。浮游生物、ＣＯＤ、ＮＨ＋４－Ｎ及ＰＯ３－４－Ｐ与细菌数之间均无明显相关性。细菌数在水库表、底水层未见显著差异。养殖季细菌的平均世代时间为５２ｈ,日生长常数（ｋ）０．３８,细菌生产量１．５９ｍｇ／ｌ·ｄ,占浮游植物初级产量的２７％。养殖季细菌可提供４．７６ｇ／ｍ３的鱼产量。     

黑曲霉生产β—葡萄糖苷酶发酵条件的研究

经多项式回归分析,研究了不同浓度Ｎ源、Ｃ源、无机盐等对酶产量的影响,确定出最佳培养基配方为：麸皮４．９％,（ＮＨ４）２ＳＯ４０．４％,ＫＨ２ＰＯ４０．２９％,ＣａＣｌ２０．０５％,ＭｇＳＯ４·７Ｈ２Ｏ０．０４％,ＦｅＳＯ４·７５ｍｇ·Ｌ＾－ＺｎＣｌ２１．４ｍｇ·Ｌ＾－,０．２％油酸钠。并对培养温度１时间、培养基初妈ｐＨ、通气量、接种量、接种方式等培养条件进行优化,使黑曲霉生产β－葡萄糖苷酶的产量由     

银王亮丝草的试管微繁殖

１植物名称银王亮丝草（Ａｇｌａｏｎｅｍａ　ｃｏｍ－ｍｕｔａｔｕｍｃｖ．"ＳｉｌｖｅｒＫｉｎｇ”）,又名银王粗肋草、银王万年青、银皇帝。２材料类别带腋芽的茎段。３培养条件以ＭＳ为基本培养基。芽诱导培养基附加６－ＢＡ２．０～２．５ｍｇ·Ｌ－１（单位下同）;芽增殖培养基附加６－ＢＡ４．０～４．５及ＮＡＡ０．０１～０．０２;生根培养基采用３／４ＭＳ附加ＩＢＡ３．０～３．５及ＧＡ３３,０。所有培养基蔗糖及琼脂含量分别为３％和０．７５％,ｐＨ值均调至５．８。培养温度２６～２８℃,光照８～１０ｈ·ｄ－１,光照…     

环境条件对水网藻(Hydrodictyon reticulatum)生长的影响

研究了温度、光照强度及Ｎ、Ｐ浓度与水网藻生长的关系．结果表明,水网藻能在较大的温度范围（１０～３１℃）、光照强度（３００～５３００Ｌｘ）和Ｎ浓度（１．０～８４．０ｍｇ·Ｌ－１）和Ｐ浓度（０．０５～３．７２ｍｇ·Ｌ－１）等条件下生长．最适生长温度为２５℃,当光照强度大于１３００Ｌｘ,Ｎ、Ｐ含量分别为１６．８～５０．４和０．７４４～２．２３ｍｇ·Ｌ－１时,水网藻生长良好．低温有利于水网藻忍耐低光照和低Ｎ低Ｐ．     

用国产微孔玻璃珠初步纯化人u-PA

用国产微孔玻璃珠从ＣＤ－１工程细胞株培养上清中纯化ｕ－ＰＡ,摸索了微孔玻璃珠结合ｕ－ＰＡ的条件和洗脱方法,比较了硫酸铵线性梯度洗脱或２５％乙二醇洗脱ｕ－ＰＡ活性的结果,最后确定洗脱的条件为０．２５ｍｏｌ／ＬＴｒｉｓ、１～２ｍｏｌ／Ｌ（ＮＨ４）２ＳＯ４、ｐＨ９．３。经此一步纯化,ｕ－ＰＡ比活性达到１５３２９ＩＵ／ｍｇ,回收率７８％,还原条件下ＳＤＳ－ＰＡＧＥ分子量为５２×１０３的单链ｕ－ＰＡ占７４％。     

少动鞘脂单胞菌S1胞外多糖发酵工艺条件研究

研究了摇瓶培养条件下少动鞘脂单胞菌引的胞外多糖的发酵工艺。Ｓ１菌的发酵产胶可采用二步发酵法：第一阶段,培养基成分为：蔗糖１０ｇ,ＮＨ_４ＮＯ_３　０．５ｇ,ＫＨ_２ＯＰ_４　０．５ｇ,ＭｎＳＯ_４　０．３ｇ,ＭｇＳＯ_４　０．１ｇ,吐温８０ ０．０２ｇ溶于蒸馏水并定容至１０００ｍＬ,ｐＨ７．２±０．１,温度３３℃～３５℃,高溶氧。第二阶段,发酵 １０～１２ｈ后,补加蔗糖４０ｇ／Ｌ,温度     

铵态和硝态氮对甜椒生理效应的比较(简报)

甜椒幼苗期优先吸收ＮＨ_４~＋－Ｎ,ＮＨ_４~＋－Ｎ对幼苗的促长作用大于ＮＯ_３~－－Ｎ,而结果期则优先吸收ＮＯ_３~－－Ｎ。ＮＨ_４~＋－Ｎ与ＮＯ_３~－－Ｎ均显著增力。叶片中叶绿素含量、光合速率及硝酸还原酶活性,有利于干物质积累,最终提高产量。     

SPHINGOLIPIDS AND PHOSPHOLIPIDS IN MICROSOMES AND MYELIN FROM NORMAL AND PATHOLOGICAL BRAINS

—Myelin and microsomes were separated from human cerebral white matter and cortex respectively using the technique of 15% caesium chloride and their sphingolipid and phospholipid contents estimated. Normal brains as well as cerebral material from cases of metachromatic leucodystrophy, Krabbe's disease and Tay-Sachs’disease were studied. Gangliosides were not present in normal myelin but were found in microsomes and in myelin from the pathological material. The ratio of cerebroside to sulphatide in myelin was 4 to 1 in normal, 1 to 20 in metachromatic and 7 to 1 in Krabbe's disease. The results in the human material are briefly discussed.     

HIGH AND LOW DENSITY WATER AND RESTING,ACTIVE AND TRANSFORMED CELLS

Resting and active states of cells are described in terms of the expectation, derived from experiments with aqueous polymers, that they contain two modified forms of water: high density, reactive, fluid water and low density, inert, viscous water. Low density water predominates in a resting cell and is converted to high density water in an active cell. It is proposed that switching from one state to another is an integral part of cellular function. When this ability is lost cells are transformed either to a state of rigor or to a hyperactive state in which they no longer depend upon external signals.