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1.
N. P. Balaban N. L. Rudakova A. R. Sabirova L. R. Valeeva M. R. Sharipova 《Russian Journal of Bioorganic Chemistry》2012,38(4):383-391
Heterologous gene expression of the extracellular minor metalloendopeptidase of Bacillus pumilus 3?C19 (AN EU678894) in the protease-deficient B. pumilus 3?C19 strain has been studied. The enzyme fraction in the total pool of B. pumilus 3?C19 proteases is less than 8%. The enzyme was isolated from the culture liquid of the recombinant strain and purified to a homogeneous state; its primary structure was determined and its physicochemical properties were investigated. The secreted metalloendopeptidase of B. pumilus 3?C19 is the first prokaryotic homologue of the eukaryotic adamalysin/reprolysin protein family. 相似文献
2.
Le H. Duc Huynh A. Hong Teresa M. Barbosa Adriano O. Henriques Simon M. Cutting 《Applied microbiology》2004,70(4):2161-2171
Bacillus species (Bacillus cereus, Bacillus clausii, Bacillus pumilus) carried in five commercial probiotic products consisting of bacterial spores were characterized for potential attributes (colonization, immunostimulation, and antimicrobial activity) that could account for their claimed probiotic properties. Three B. cereus strains were shown to persist in the mouse gastrointestinal tract for up to 18 days postadministration, demonstrating that these organisms have some ability to colonize. Spores of one B. cereus strain were extremely sensitive to simulated gastric conditions and simulated intestinal fluids. Spores of all strains were immunogenic when they were given orally to mice, but the B. pumilus strain was found to generate particularly high anti-spore immunoglobulin G titers. Spores of B. pumilus and of a laboratory strain of B. subtilis were found to induce the proinflammatory cytokine interleukin-6 in a cultured macrophage cell line, and in vivo, spores of B. pumilus and B. subtilis induced the proinflammatory cytokine tumor necrosis factor alpha and the Th1 cytokine gamma interferon. The B. pumilus strain and one B. cereus strain (B. cereus var. vietnami) were found to produce a bacteriocin-like activity against other Bacillus species. The results that provided evidence of colonization, immunostimulation, and antimicrobial activity support the hypothesis that the organisms have a potential probiotic effect. However, the three B. cereus strains were also found to produce the Hbl and Nhe enterotoxins, which makes them unsafe for human use. 相似文献
3.
H Kersters-Hilderson M Claeyssens E van Doorslaer C K de Bruyne 《Carbohydrate research》1976,47(2):269-273
The anomeric configuration of D-xylose, resulting from hydrolysis of β-D xylopyranosides by β-D-xylosidase from Bacillus pumilus, has been determined by an enzymic procedure, based on the stereospecificity of D-xylose isomerases. The initial hydrolysis product is α-D-xylose. β-D-Xylosidase from Bacillus pumilus thus acts by inversion of configuration in contrast to most other glycosidases. 相似文献
4.
Christiane Brack Annett Mikolasch Frieder Schauer 《Marine biotechnology (New York, N.Y.)》2014,16(4):385-395
We report the detection by gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry analyses of the secreted 2,5-diketopiperazines (DKPs) cyclo(-Ala-Pro), cyclo(-Gly-Pro), cyclo(-Val-Pro), cyclo(-Ile-Pro), cyclo(-Leu-Pro), cyclo(-Pro-Pro), cyclo(-HyP-Pro), cyclo(-Met-Pro), and cyclo(-Phe-Pro) produced by Bacillus pumilus. The study focuses on a marine isolate and a laboratory test strain of B. pumilus with capabilities to lyse pregrown living cell lawns of different bacterial species, among them Arthrobacter citreus. Chromatographic methods were used to analyze induced bioactive compounds. At least 13 different DKPs are produced by B. pumilus. Both strains respond with an increased production of the DKPs cyclo(-Gly-Pro), cyclo(-Ala-Pro), and cyclo(-Val-Pro) to the presence of pasteurized A. citreus cells after 4 h in a nutrient-poor liquid medium. In agar diffusion assays, these DKPs did not cause lysis zones in living cell lawns, but they did inhibit further growth of several pregrown test bacteria in microplates even at concentrations as low as 1 μg ml?1. Antibiotic substances produced by B. pumilus after 20 h of cultivation in a special lysis medium showed lytic activity in cell-free extracts of B. pumilus culture supernatants. 相似文献
5.
《Journal of Asia》2022,25(4):102005
Acrobasis nuxvorella Neuzing (Lepidoptera: Pyralidae), or the Pecan Nut Casebearer (PNC), is a monophagous pecan nut [Carya illinoinensis (Wang) K. Koch] herbivore. This insect has caused major crop losses, despite pecan nut trees producing a high concentration of tannins, which are deterrent compounds for insects. The PNC consumes and processes tannin-rich tissues without any negative effects on its nutrition. Certain bacterial species of the herbivore gut microbiota have been proven to produce tannase, therefore, we hypothesize that the PNC has symbiotic bacteria that produce tannase for the digestion of tannin contained in their food.In this work, live PNC larvae were extracted from pecan nutlets. The larval guts were dissected and their contents were cultured to obtain the cultivable bacteria. A total of 224 bacterial isolates were recovered, 19 of which tested positive for tannase activity. Isolates LB66, LB24, TT29, and TP8 displayed comparable activity to the control strain Pseudomonas aeruginosa. Further enzyme semi-purification steps showed specific tannase activity values in the range of 39.5–3.7 U/mg of protein. The isolates were identified as Bacillus pumilus strain TT29, Bacillus pumilus strain LB66, Bacillus pumilus strain LB24 and Sthaphylococcus warneri (TP8) by 16S ribosomal RNA gene sequencing. Our findings indicate that PNC larvae contain gut bacteria able to produce tannase that hydrolyze the galloyl ester bonds in tannins. 相似文献
6.
Raquel Branquinho Clara Sousa Jo?o Lopes Manuela E. Pintado Luísa V. Peixe Hugo Osório 《PloS one》2014,9(10)
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) despite being increasingly used as a method for microbial identification, still present limitations in which concerns the differentiation of closely related species. Bacillus pumillus and Bacillus safensis, are species of biotechnological and pharmaceutical significance, difficult to differentiate by conventional methodologies. In this study, using a well-characterized collection of B. pumillus and B. safensis isolates, we demonstrated the suitability of MALDI-TOF-MS combined with chemometrics to accurately and rapidly identify them. Moreover, characteristic species-specific ion masses were tentatively assigned, using UniProtKB/Swiss-Prot and UniProtKB/TrEMBL databases and primary literature. Delineation of B. pumilus (ions at m/z 5271 and 6122) and B. safensis (ions at m/z 5288, 5568 and 6413) species were supported by a congruent characteristic protein pattern. Moreover, using a chemometric approach, the score plot created by partial least square discriminant analysis (PLSDA) of mass spectra demonstrated the presence of two individualized clusters, each one enclosing isolates belonging to a species-specific spectral group. The generated pool of species-specific proteins comprised mostly ribosomal and SASPs proteins. Therefore, in B. pumilus the specific ion at m/z 5271 was associated with a small acid-soluble spore protein (SASP O) or with 50S protein L35, whereas in B. safensis specific ions at m/z 5288 and 5568 were associated with SASP J and P, respectively, and an ion at m/z 6413 with 50S protein L32. Thus, the resulting unique protein profile combined with chemometric analysis, proved to be valuable tools for B. pumilus and B. safensis discrimination, allowing their reliable, reproducible and rapid identification. 相似文献
7.
Bacillus pumilus was isolated from surface-sterilized tissues of the medicinal plant Ocimum sanctum. Scanning electron microscopic (SEM) imaging confirmed the presence of a rod shaped bacterium within the plant tissues. The bacterium was identified as B. pumilus by biochemical analyses and 16S rRNA gene sequencing. In vitro analyses indicate that the isolated strain of B. pumilus was endowed with multiple plant growth promotion (PGP) traits such as phosphate solubilization and the production of indole acetic acid (IAA), siderophore and hydrogen cyanide (HCN). Phosphate solubilization (37.3 μg ml?1) and IAA production (36.7 μg ml?1) by the isolate was found to reach a maximum after 60 h of incubation. Siderophore mediated iron sequestration by B. pumilus may confer a competitive advantage to the host with respect to pathogen inhibition. Siderophore produced by the isolate was found to be of a trihydroxamate type with hexadentate nature. The B. pumilus isolate also exhibited cellulolytic, proteolytic and chitinolytic activity. Cell free supernatant, culture filtrates of the isolate were found to suppress the growth of fungal phytopathogens. The culture filtrate retained its antifungal activity even after exposure to heat. In addition to PGP, the isolate exhibited probiotic properties such as acid tolerance (pH2), bile salt tolerance (2 %), auto-aggregation, antibiotic resistance and the absence of haemolytic activity. These finding suggest the possibility of utilizing this endophytic strain of B. pumilus as a bioinoculant to enhance plant growth and also as a probiotic. 相似文献
8.
Jeyaram K Romi W Singh TA Adewumi GA Basanti K Oguntoyinbo FA 《Journal of microbiological methods》2011,87(2):161-164
PCR amplification of 16S rRNA gene by universal primers followed by restriction fragment length polymorphism analysis using RsaI, CfoI and HinfI endonucleases, distinctly differentiated closely related Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus from Bacillus subtilis sensu stricto. This simple, economical, rapid and reliable protocol could be an alternative to misleading phenotype-based grouping of these closely related species. 相似文献
9.
10.
Use of a Plasmid DNA Probe To Monitor Populations of Bacillus pumilus Inoculant Strains in Hay 总被引:2,自引:0,他引:2
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Carol A. Hendrick Brenda K. Smiley Terrence H. Shelley Nancy J. Tomes 《Applied microbiology》1991,57(3):686-693
We are evaluating naturally occurring isolates of Bacillus pumilus for use as microbial hay preservatives. Seven isolates of B. pumilus from hay contained a 42-kb cryptic plasmid (pMGD296). We wished to determine whether pMGD296 could be used as a molecular marker to follow populations of these isolates in hay over time. Southern blots and colony blots of 69 isolates of B. pumilus and other Bacillus spp. were probed with 32P-labeled pMGD296. Twenty-nine probe-positive isolates were identified; of these, 28 contained a plasmid with a restriction profile identical to that of pMGD296. One isolate from untreated hay contained a 40-kb plasmid (pMGD150) that was homologous to pMGD296 but had a different restriction fragment pattern. Regions of homology between the two plasmids were identified by Southern blotting, and a 1.9-kb HindIII-PstI fragment of pMGD296 lacking strong homology to pMGD150 was cloned in pUC18. The cloned fragment hybridized only with isolates containing pMGD296 and was used to estimate populations of these isolates in treated and untreated hay. 相似文献
11.
Survival of Spacecraft-Associated Microorganisms under Simulated Martian UV Irradiation 总被引:5,自引:2,他引:3
David A. Newcombe Andrew C. Schuerger James N. Benardini Danielle Dickinson Roger Tanner Kasthuri Venkateswaran 《Applied microbiology》2005,71(12):8147-8156
Spore-forming microbes recovered from spacecraft surfaces and assembly facilities were exposed to simulated Martian UV irradiation. The effects of UVA (315 to 400 nm), UVA+B (280 to 400 nm), and the full UV spectrum (200 to 400 nm) on the survival of microorganisms were studied at UV intensities expected to strike the surfaces of Mars. Microbial species isolated from the surfaces of several spacecraft, including Mars Odyssey, X-2000 (avionics), and the International Space Station, and their assembly facilities were identified using 16S rRNA gene sequencing. Forty-three Bacillus spore lines were screened, and 19 isolates showed resistance to UVC irradiation (200 to 280 nm) after exposure to 1,000 J m−2 of UVC irradiation at 254 nm using a low-pressure mercury lamp. Spores of Bacillus species isolated from spacecraft-associated surfaces were more resistant than a standard dosimetric strain, Bacillus subtilis 168. In addition, the exposure time required for UVA+B irradiation to reduce the viable spore numbers by 90% was 35-fold longer than the exposure time required for the full UV spectrum to do this, confirming that UVC is the primary biocidal bandwidth. Among the Bacillus species tested, spores of a Bacillus pumilus strain showed the greatest resistance to all three UV bandwidths, as well as the total spectrum. The resistance to simulated Mars UV irradiation was strain specific; B. pumilus SAFR-032 exhibited greater resistance than all other strains tested. The isolation of organisms like B. pumilus SAFR-032 and the greater survival of this organism (sixfold) than of the standard dosimetric strains should be considered when the sanitation capabilities of UV irradiation are determined. 相似文献
12.
研究了短小芽孢杆菌(Bacillus pumilus)对盐藻空间诱变株系SZ-05(Dunaliella salina SZ-05)的生物量及β-胡萝卜素积累的影响。结果表明,短小芽孢杆菌显著提高了盐藻SZ-05的生物量和β-胡萝卜素的产量,明显降低了培养体系中的溶解氧和胞外多糖的含量。溶解氧的减少,使得藻细胞的光呼吸作用下降,光合作用速率提高,使藻细胞生物量增加。胞外多糖具有抗氧化作用,胞外多糖的减少可能进一步增加了β-胡萝卜素的合成,从而使β-胡萝卜素在胁迫条件下大幅度增加。 相似文献
13.
Bacillus pumilus Reveals a Remarkably High Resistance to Hydrogen Peroxide Provoked Oxidative Stress
Stefan Handtke Rebecca Schroeter Britta Jürgen Karen Methling Rabea Schlüter Dirk Albrecht Sacha A. F. T. van Hijum Johannes Bongaerts Karl-Heinz Maurer Michael Lalk Thomas Schweder Michael Hecker Birgit Voigt 《PloS one》2014,9(1)
14.
《Aquatic Botany》2007,86(3):243-252
Reeds may play important role in the self-purification of aquatic habitats due to the filtration capacity and their periphyton communities developing on the underwater plant surfaces. The efficiency of this process and the transformation of organic substances can be influenced by the species composition and activity of microbial communities, including Bacillus and related species. For cultivation based bacteriological examinations reed periphyton samples were collected from 30 cm beneath the water surface of Lake Velencei and the Soroksár Danube branch (Hungary). After a primary selection 40 Bacillus and related strains were investigated by traditional morphological, biochemical tests, API 20E, API 50CHB and BIOLOG GP2 systems, and identified by 16S rDNA sequence comparison. The isolated strains were characterized by wide biochemical activity spectrum (i.e., the metabolism of carbohydrates and biopolymers) as well as widespread ecological tolerance based on their NaCl and pH range investigations. Species with facultative alkaliphilic features (Marinibacillus marinus, Bacillus firmus) were detected only from the reed biofilm of Lake Velencei, while alkalitolerant Bacillus and related species from both sampling sites. 22 endospore-forming strains were identified as members of species B. cereus, B. firmus, B. flexus, B. licheniformis, B. megaterium, B. muralis, B. pumilus, B. subtilis and Marinibacillus marinus. Only one species, with 95–96% sequence similarities to B. pumilus was found to be common among the strains from Lake Velencei and the Soroksár Danube branch. Altogether, 18 strains could not be identified as known species of Bacillus, Brevibacillus and Paenibacillus, hence they may represent new bacterial taxa. 相似文献
15.
GUSTAVO YA
EZ-OCAMPO MARTHA E. MORA-HERRERA ARNOLDO WONG-VILLARREAL DENISSE M. DE LA PAZ-OSORIO NADIA DE LA PORTILLA-L
PEZ JORGE LUGO ROCIO VACA-PAULÍN PEDRO DEL GUILA 《Polish journal of microbiology》2020,69(3):357
The capacity of four bacterial strains isolated from productive soil potato fields to solubilize tricalcium phosphate on Pikovskaya agar or in a liquid medium was evaluated. A bacterial strain was selected to evaluate in vitro capacity of plant-growth promotion on Solanum tuberosum L. culture. Bacterial strain A3 showed the highest value of phosphate solubilization, reaching a 20 mm-diameter halo and a concentration of 350 mg/l on agar and in a liquid medium, respectively. Bacterial strain A3 was identified by 16S rDNA analysis as Bacillus pumilus with 98% identity; therefore, it is the first report for Bacillus pumilus as phosphate solubilizer. Plant-growth promotion assayed by in vitro culture of potato microplants showed that the addition of bacterial strain A3 increased root and stems length after 28 days. It significantly increased stem length by 79.3%, and duplicated the fresh weight of control microplants. In this paper, results reported regarding phosphorus solubilization and growth promotion under in vitro conditions represent a step forward in the use of innocuous bacterial strain biofertilizer on potato field cultures.Key words: Bacillus sp., phosphorus soluble, Pikovskaya agar, potato rhizosphere, plant growth promoting rhizobacteria 相似文献
16.
The biological effects of ribonucleases (RNases), such as the control of the blood vessels growth, the toxicity towards tumour cells and antiviral activity, require a detailed explanation. One of the most intriguing properties of RNases which can contribute to their biological effects is the ability to form dimers, which facilitates efficient RNA hydrolysis and the evasion of ribonuclease inhibitor. Dimeric forms of microbial RNase binase secreted by Bacillus pumilus (former B. intermedius) have only been found in crystals to date. Our study is the first report directly confirming the existence of binase dimers in solution and under natural conditions of enzyme biosynthesis and secretion by bacilli. Using different variants of gel electrophoresis, immunoblotting, size-exclusion chromatography and mass-spectrometry, we revealed that binase is a stable natural dimer with high catalytic activity. 相似文献
17.
《Journal of Fermentation and Bioengineering》1991,71(5):303-308
Xylanase (xynA) and β-xylosidase (xynB) genes of Bacillus pumilus were expressed in Saccharomyces cerevisiae by using the GAP (glyceraldehyde-3-phosphate dehydrogenase) promoter of S. cerevisiae. Yeast cells harboring a plasmid pNAX2 containing xynA produced xylanase in the cytoplasm of the cell to an extent as much as 5% of the total soluble protein in the cell extract. Xylanase produced in yeast had an extra methionine at the N-terminus, but had the same specific activity as that produced by B. pumilus IPO. The xylanase in the yeast was not glycosylated and was immunologically identical to that of B. pumilus IPO. Yeast cells harboring a plasmid pYXB containing xynB produced β-xylosidase in the cytoplasm of the cell (3% of the total soluble protein). β-Xylosidase purified from the yeast strain exhibited specific activity nearly equal to the value of enzyme purified from B. pumilus, and had an N-terminal sequence identical to the sequence of the enzyme from B. pumilus. 相似文献
18.
Bacillus pumilus BA06 was isolated from the proteinaceous soil and produced an extracellular alkaline protease with leather-dehairing function. The genome of BA06 was sequenced. The comparative genome analysis indicated that strain BA06 is different in genome from the other B. pumilus strains, with limited insertions, deletions, and rearrangements. 相似文献
19.
Checinska A Fruth IA Green TL Crawford RL Paszczynski AJ 《Journal of microbiological methods》2011,87(1):70-75
Novel noninvasive techniques for the removal of biological contaminants to generate clean or sterile materials are in demand by the medical, pharmaceutical and food industries. The sterilization method described here uses supercritical fluid carbon dioxide (SF-CO2) containing 3.3% water and 0.1% hydrogen peroxide (v/v/v) to achieve from four to eight log viability reduction of all tested microbial species, including vegetative cells, spores and biofilms. The sterilization method employs moderate pressure and temperature (80 atm, 50 °C) and a short (30-minute) treatment time. The procedure kills various opportunistic pathogens that often persist in biofilm structures, fungal spores commonly associated with nosocomial infections, and Bacillus pumilus SAFR-032 endospores that are notoriously hard to eradicate by conventional sterilization techniques. 相似文献
20.
M. L. Vijaya Kumar B. Thippeswamy P. Vasanth Raj 《Applied Biochemistry and Microbiology》2014,50(6):619-623
The metabolites of bacteria Bacillus cereus and Bacillus pumilus isolated from soil samples in Shimoga region, Karnataka (India) were tested for cytotoxicity and anticancer properties. The various solvent extract fractions obtained from the metabolites of the two bacteria were tested for their cytotoxicity against normal human liver cell lines and 2 cancer cell lines by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) assay. The two fractions obtained from B. cereus showed high cytotoxicity. These two fractions were further screened for anticancer activity by nuclear staining studies and DNA fragmentation analysis. Both the fractions demonstrated significant activity by membrane blebbing during nuclear staining and caused the damage the DNA patterns during DNA fragmentation analysis. On the other hand, the metabolites of B. pumilus revealed toxic effect against cancer cells as well as normal ones. 相似文献