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Meyer V Spielvogel A Funk L Tilburn J Arst HN Stahl U 《Molecular genetics and genomics : MGG》2005,274(3):295-306
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Nozawa SR Ferreira-Nozawa MS Martinez-Rossi NM Rossi A 《Fungal genetics and biology : FG & B》2003,39(3):286-295
In this communication, we show that the pacC(c)14 mutation drastically reduced the mannose and N-acetylglycosamine content of the pacA-encoded acid phosphatase secreted by the fungus Aspergillus nidulans when grown at 22 degrees C, pH 5.0, compared to a control strain. The staining after PAGE was not observed for the pacA-encoded acid phosphatase, while the palD-encoded Pi-repressible alkaline phosphatase had an altered electrophoretic mobility. In addition, the secreted acid phosphatase also had a reduced number of isoforms visualized by staining after IEF and glycosylation had a protective effect against its heat inactivation. We also show that a full-length version of gene pacC-1 cloned from Neurospora crassa complemented the pacC(c)14 mutation of A. nidulans, including the remediation of both the acid and alkaline Pi-repressible phosphatases secreted at pH 5.0, which indicates that glycosylation of secreted phosphatases is mediated in A. nidulans by the conserved PacC pathway that governs pH-responsive gene expression. 相似文献
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Ambient pH gene regulation in fungi: making connections 总被引:1,自引:0,他引:1
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