Highlights► We focus on the latest developments in nanotechnology-based electrophysiology. ► Methods for extracellular recording via nanoFET-based devices are described. ► Metal nanoelectrodes for the recording of electrogenic cells are reviewed. ► The latest developments of intracellular FET-based probes are shortly covered. ► Pioneering electrical scaffold devices for the interfacing with tissues are described. 相似文献
Highlights? Small, sneaky squid males produce spermatozoa that swarm to CO2 ? Sneaker, but not consort, sperm acidify their intracellular pH due to extracellular acidification by carbonic anhydrase ? Recovery from intracellular acidosis evokes Ca2+-dependent chemotactic swarming ? Swarming by sneaker sperm might be an evolutionary adaptation to mating tactics 相似文献
Highlights► Microbial production of pharmaceutical and fuel molecules. ► Systems biology tools advanced our understanding about synthetic pathway design. ► Synthetic biology led us to cell factories tailor-made for chemical production. ► Combinatorial gene assembly for synthetic pathway diversification. ► Engineering static, dynamic and spatial control for better production phenotype. 相似文献
Highlights? PS is expressed on the surface of apoptotic and phagocytic cells during apoptosis ? CED-7 and TTR-52 mediate time-dependent loss of PS from surface of apoptotic cells ? CED-7 and TTR-52 promote generation of extracellular PS vesicles ? CED-7/TTR-52/CED-1 promote phagocyte PS expression, important for corpse engulfment 相似文献
Highlights? Structural insights into the complete extracellular human IL-34:CSF-1R complex ? The C-terminal tail of hIL-34 is flexible and heavily O-linked glycosylated ? Inclusion of N-linked glycans drastically improves fitting of models to SAXS data ? Consensus principles for hCSF-1R activation by its two distinct cytokine ligands 相似文献
Highlights? Microbial genomes contain multiple clusters for biosynthesis of chemically diverse compounds. ? Most of such genes remain silent, preventing the respective compounds from being discovered. ? Silent gene clusters can be activated and/or heterologously expressed. ? New microbial hosts and tools are needed to make the abovementioned process efficient. ? Synthetic biology will play a major role in the genome-based drug discovery. 相似文献
Highlights► The application of Pichia pastoris for biopharmaceutical production is described. ► Synthetic biology approaches and perspectives to improve production are reviewed. ► Glycoengineering efforts to produce humanized, uniform glycoproteins are covered. ► The design and application of synthetic promoter variants are highlighted. ► The molecular toolbox available for synthetic biology in P. pastoris is discussed. 相似文献
Highlights? HIV-Nef translocates activated TACE/ADAM17 into extracellular vesicles (EVs) ? The mechanism is initiated by interaction of Eed and paxillin with TACE ? Translocation of TACE into EVs is regulated by Pak2 ? Melanoma cells activate the same mechanism to upload activated ADAM10 into EVs 相似文献
Highlights► Nanotechnologies are used to mimic natural components of the cell microenvironment. ► Inorganic nanomaterials interact with cells to promote physiological processes. ► Nanoelectronic devices are integrated with engineered tissues to monitor function. 相似文献
•Highly glycosylated matrisome proteins and their binding partners comprise extracellular networks that mediate tissue-specific cellular microenvironments.
•Elucidation of roles of matrisome molecules in disease mechanisms requires detailed mapping of matrisome glycosylation and other post-translational modifications.
•We review tissue workup methods for matrisome proteomics, glycomics and glycoproteomics.
•The combination of proteomics, glycomics and glycoproteomics profiles matrisome protein modifications distinct from those studied by immunohistochemistry.
Highlights? A high-content chemical screen identifies modulators of intracellular mycobacteria ? Three compounds that kill bacteria by altering host processes were validated ? Multiparametric chemical and genetic data sets integrated to identify mode of action ? Modulation of autophagy and endosomal trafficking identified as mode of action 相似文献
