Bacterial community composition in low-flowing river water with different sources of pollutants

Pollution of water resources is a major risk to human health and water quality throughout the world. The purpose of this study was to determine the influence of pollutant sources from agricultural activities, urban runoffs, and runoffs from wastewater treatment plants (WWTPs) on bacterial communities in a low-flowing river. Bacterial community structure was monitored using terminal restriction fragment length polymorphism (T-RFLP) and 16S rRNA gene clone library. The results were analyzed using nonmetric multidimensional scaling (NMDS) and UniFrac, coupled with principal coordinate analysis (PCoA) to compare diversity, abundance, community structure, and specific functional groups of bacteria in surface water affected by nonpoint sources. From all the sampling points, Bacteria were numerically dominated by three phyla – the Proteobacteria, Bacteroidetes, and Cyanobacteria – accounting for the majority of taxa detected. Overall results, using the b diversity measures UniFrac, coupled with PCoA, showed that bacterial contamination of the low-flowing river was not significantly different between agricultural activities and urban runoff.     

Life Cycle Assessment of Biosolids Land Application and Evaluation of the Factors Impacting Human Toxicity through Plant Uptake

Due to increasing environmental concerns in the wastewater treatment sector, the environmental impacts of organic waste disposal procedures require careful evaluation. However, the impacts related to the return of organic matter to agricultural soils are difficult to assess. The goals of this study are to assess the environmental impacts of land application of two types of biosolids (dried and composted, respectively) from the same wastewater treatment plant in France, and to improve the quantification of human toxicity. A life cycle assessment (LCA) was carried out on a case study based on validated data from an actual wastewater treatment plant. Numerous impacts were included in this analysis, but a particular emphasis was laid on human toxicity via plant ingestion. For six out of the eight impact categories included in the analysis, the dried biosolids system was more harmful to the environment than the composting route, especially regarding the consumption of primary energy. Only human toxicity via water, soil, and air compartments and ozone depletion impacts were higher with the composted biosolids.     

Biofilms and their impact on the food industry

Biofilm could be defined as a complex communities of microorganisms seen affixed to surfaces, they form clusters without sticking to any surface and buried firmly in an extracellular matrix (ECM). This matrix is formed by microorganisms in the formation of either extracellular polymeric substances (EPSS) or extracellular polymer. Many reviews have addressed the negative consequences of biofilm production in the food industry, among which we talk about biofilms being responsible for spoilage microorganisms and foodborne pathogens such as Listeria monocytogenes, Bacillus cereus etc. These contamination could be linked to biofilms presence in the processing plant. Although researches have tried conferring solutions to these challenges in the food industry, however, in this review we have tried to focus on the positive impact of biofilms formed in the food industry. It is critically expedient while trying to find the solution to the challenges of biofilm in the food industry to develop and give a major focus on the advantages and positive impact biofilm has in the food industry, which has been greatly neglected. Hence in this article, we have highlighted some positive impacts of biofilms formed in the food industry, like enhancing plant health and productivity of food products, as an agent of water and wastewater treatment in the food industry, as a tool in reducing the amount of excess sludge in the wastewater treatment plant. The development of edible biofilms, fermented food products and the production of biodegradable food packaging are also part of biofilms beneficial roles in the food industries.     

Response of stream macroinvertebrate assemblages to erosion control structures in a wastewater dominated urban stream in the southwestern U.S.

Effects of stream erosion control structures on aquatic macroinvertebrates were studied (2000–2009) in a wastewater dominated drainage (Wash) in Las Vegas, Nevada. Mainstem sites with and without structures, wastewater treatment plant outfalls, a reference site above treatment plant inputs, and tributary sites were sampled. Ordination suggested hydrology and channel characteristics (current velocity, stream depth, and width), and water quality (conductivity) were primary factors in organizing macroinvertebrate communities, with some variables altered at structures. Treatment plant inputs changed hydrology (increased flows), water chemistry (conductivity decreased below treatment plants), and temperature. Assemblages differed between site types, with midges and damselflies important at tributary sites and Fallceon mayflies and Smicridea caddisflies common at erosion control structures. Locally unique communities developed at structures which also may have facilitated exotic species invasions. Analyses showed that taxa richness increased over time at these sites and differed significantly from richness at sites without structures. Structures appeared important in retaining organic matter and, among mainstem sites, coarse particulate organic matter was highest, but variable, at structures and at wetlands above the structures. Erosion control structures, coupled with warm effluent, high baseflows, and altered water quality resulted in development of a macroinvertebrate community that did not trend towards reference or tributary sites. In this case, ecological communities at structures used for river restoration were not on a continuum between disturbed and reference sites. Goal setting of community responses at these structures would have required insight beyond the simple use of reference site attributes.     

An off-line filtering ditch–pond system for diffuse pollution control at Wuhan City Zoo

An off-line filtering ditch–pond system was designed and constructed to control the small point and runoff pollution at the Wuhan City Zoo, Hubei Province, China. The quantity and quality of wastewater discharge and runoff from 16 rainfall events were measured to test the effectiveness of the off-line treatment train. The results showed that the water quality was improved and high retention rates for water and pollutants were also achieved by the off-line treatment train. In the outflows, the event mean concentrations (EMCs) of TSS, COD, TN, TDN, TP and TDP were reduced by 75%, 50%, 50%, 57%, 74% and 80% compared to the inflows. In 2005, the annual inflow volume in the catchment was 6783 m³ and the water retention rate was 80.1%. The retention rates in the annual loads of TSS, COD, TN and TP came to 86.4%, 85.5%, 83.9% and 82.9%, respectively. Therefore, the off-line filtering ditch–pond system was shown to be an effective and economical measure to control diffuse pollution. It would be worthwhile to extend the off-line treatment train to regions with limited land resources, especially in urban areas.     

The Water Impact Index: a simplified single-indicator approach for water footprinting

Purpose

Along with climate change-related issues, improved water management is recognized as one of the major challenges to sustainability. However, there are still no commonly accepted methods for measuring sustainability of water uses, resulting in a recent proliferation of water footprint methodologies. The Water Impact Index presented in this paper aims to integrate the issues of volume, scarcity and quality into a single indicator to assess the reduction of available water for the environment induced by freshwater uses for human activities.

Methods

The Water Impact Index follows life cycle thinking principles. For each unit process, a volumetric water balance is performed; water flows crossing the boundaries between the techno-sphere and environment are multiplied by a water quality index and a water scarcity index. The methodology is illustrated on the current municipal wastewater management system of Milan (Italy). The Water Impact Index is combined with carbon footprint to introduce multi-impact thinking to decision makers. The Water Impact Index is further compared to results obtained using a set of three life cycle impact indicators related to water, from the ReCiPe life cycle impact assessment (LCIA) methodology.

Results and discussion

Onsite water use is the main contribution to the Water Impact Index for both wastewater management schemes. The release of better quality water is the main driver in favour of the scenario including a wastewater treatment plant, while the energy and chemicals consumed for the treatment increase the indirect water footprint and carbon footprint. Results obtained with the three midpoint indicators depict similar tendencies to the Water Impact Index.

Conclusions

This paper presents a simplified single-indicator approach for water footprinting, integrating volume, scarcity and quality issues, representing an initial step toward a better understanding and assessment of the environmental impacts of human activities on water resources. The wastewater treatment plant reduces the Water Impact Index of the wastewater management system. These results are consistent with the profile of the three midpoint indicators related to water from ReCiPe.     

Organic amendment additions to rangelands: A meta‐analysis of multiple ecosystem outcomes

Interest in land application of organic amendments—such as biosolids, composts, and manures—is growing due to their potential to increase soil carbon and help mitigate climate change, as well as to support soil health and regenerative agriculture. While organic amendments are predominantly applied to croplands, their application is increasingly proposed on relatively arid rangelands that do not typically receive fertilizers or other inputs, creating unique concerns for outcomes such as native plant diversity and water quality. To maximize environmental benefits and minimize potential harms, we must understand how soil, water, and plant communities respond to particular amendments and site conditions. We conducted a global meta‐analysis of 92 studies in which organic amendments had been added to arid, semiarid, or Mediterranean rangelands. We found that organic amendments, on average, provide some environmental benefits (increased soil carbon, soil water holding capacity, aboveground net primary productivity, and plant tissue nitrogen; decreased runoff quantity), as well as some environmental harms (increased concentrations of soil lead, runoff nitrate, and runoff phosphorus; increased soil CO₂ emissions). Published data were inadequate to fully assess impacts to native plant communities. In our models, adding higher amounts of amendment benefitted four outcomes and harmed two outcomes, whereas adding amendments with higher nitrogen concentrations benefitted two outcomes and harmed four outcomes. This suggests that trade‐offs among outcomes are inevitable; however, applying low‐N amendments was consistent with both maximizing benefits and minimizing harms. Short study time frames (median 1–2 years), limited geographic scope, and, for some outcomes, few published studies limit longer‐term inferences from these models. Nevertheless, they provide a starting point to develop site‐specific amendment application strategies aimed toward realizing the potential of this practice to contribute to climate change mitigation while minimizing negative impacts on other environmental goals.     

Changes in water quality and macroinvertebrate communities resulting from urban stormflows in the Provo River,Utah, U.S.A.

Short-term changes in water quality from 7 summer stormflows and long-term changes in substrates and macroinvertebrate communities resulting from urban runoff from the city of Provo, Utah, were examined from 1999–2002 in the lower Provo River. Stormflows resulted in increased total suspended solids and concentrations of dissolved copper, lead and zinc, and decreased conductivity and dissolved oxygen. The degree of change was generally in proportion to the magnitude of the storm. However, changes were temporary with water quality parameters returning to pre-storm levels within 12 hours. River substrates showed a trend of increased compaction and decreased debris dam area downstream through the urban corridor. Macroinvertebrate communities showed trends of decreased abundance and total species diversity with increasing urbanization. Compared to non-urban reaches, communities in urban reaches had few `sensitive' species and were dominated by tolerant species, particularly snails and leeches. Comparisons with previous studies show that changes in macroinvertebrate community composition in the urban reaches reflected shifts in land use during the past 15–25 years and corresponded to expected threshold levels of impact for amount of impervious surface cover.     

Effluent application to the land: Changes in soil properties and treatment potential

Four pilot land treatment systems (LTS) planted with different plant species were investigated as a means of managing wastewater in small communities. The effects of effluent application on soil properties during three years of operation are presented. LTS were planted with Eucalyptus camaldulensis, Acacia cyanophylla, Populus nigra and Arundo donax. Wastewater was pre-treated in a septic tank and applied to LTS at suitable rates to meet crop water requirements. Effluent application was found to increase soil organic matter, P and TKN content, particularly, in the topsoil but plant species had no effect on these parameters. Increases were also observed for salinity and sodium adsorption ratio which were found to depend on hydraulic loading. Winter precipitation leached the majority of the salts accumulated during the application period. Nitrates accumulated in the soil profile throughout the application period and this increase was dependent on plant species. LTS planted with A. donax showed the lowest NO₃-N concentration in soil pore water, an effect which cannot be explained by differences in application rates or plant uptake. This may imply stimulated denitrification rates induced by the rhizosphere of reeds. Effluent application also increased total and macro porosity compared to their initial values and bulk density.     

Runoff from tomato cultivation in the estuarine environment: biological effects of farm management practices

The use of plastic row covers (plastic mulch) on vegetable farms increases runoff of pesticides after rainfall events and has been linked to toxic events in adjacent tidal waters. In coastal Virginia, USA, runoff from tomato fields with plastic mulch was suspected of causing mortality of commercial hard clam larvae at a hatchery located downstream of farming operations. Concern about the putative impacts of this practice on local waters resulted in a collection of studies to: (1) determine the sensitivity of early life stages of bivalves to copper, a commonly used fungicide; (2) examine acute and chronic biological effects of runoff on tidal creeks; and (3) examine the efficacy of management practices designed to reduce the delivery of pesticides to adjacent creeks. Laboratory bioassays revealed that 48-h LC₅₀ values for embryonic clams Mulinia lateralis and Mercenaria mercenaria were 38 and 20 μg/l, respectively. In situ bioassays with Palaemonetes pugio showed that pulsed toxic conditions sometimes occur downstream of some tomato farms in plastic mulch following rainfall events. Growth, mortality rates and bioaccumulation of copper and organic pesticides in oysters were not correlated with the use of plastic mulch in watersheds. Sediment bioassays indicated potential toxicity in sediment collected downstream of some tomato fields in plastic mulch, but the effects were not consistent between years. Closer examination of management practices on the farms suggests that controlling runoff can prevent toxic impacts. Elevated levels of crop protectants measured at the outflow of farm ponds suggested that they may do little to reduce loadings of some pesticides. However, forested buffer zones and ephemeral sedimentation basins appeared to be effective in reducing pesticide concentrations in runoff and pulsed toxicity in tidal creeks.     

The Ability of Aquatic Macrophytes to Assess Fish Farm Pollution in Two Salmon Rivers

The wastewater from freshwater fish farming is responsible for important water quality modifications in receiving ecosystems. These point source pollution cause local problems for the management of the freshwater environment, especially in salmon rivers. The aim of this paper is to study the relationship between plant communities and environmental factors in two rivers basins on which seven fish farms are located and to assess the relative part of water pollution compared to other environmental factors involved in water plant distribution. The disturbance due to fish farming wastewater increased both richness and diversity of aquatic plant communities and modified the distribution of 11 aquatic macrophytes. Nevertheless, the main factors involved in the aquatic plant distribution in the whole data set were not the location upstream or downstream from one point source pollution but the belonging to one river system and the habitat physical features. Relevant prediction of macrophytic communities is a useful tool for bioindication purposes but several groups of variables that manifest themselves at different spatial levels must be considered.     

Response of benthic macroinvertebrate communities to highway construction in an Appalachian watershed

Highway construction in mountainous areas can result in sedimentation of streams, negatively impacting stream habitat, water quality, and biotic communities. We assessed the impacts of construction of a segment of Corridor H, a four-lane highway, in the Lost River watershed, West Virginia, by monitoring benthic macroinvertebrate communities and water quality, before, during, and after highway construction and prior to highway use at upstream and downstream sites from 1997 through 2007. Data analysis of temporal impacts of highway construction followed a Before-After-Control-Impact (BACI) study design. Highway construction impacts included an increase in stream sedimentation during the construction phase. This was indicated by an increase in turbidity and total suspended solids. Benthic macroinvertebrate metrics indicated a community more tolerant during and after construction than in the period before construction. The percent of Chironomidae and the Hilsenhoff Biotic Index (HBI) increased, while percent of Ephemeroptera, Plecoptera, and Trichoptera (EPT) decreased. Our 10-year study addressed short-term impacts of highway construction and found that impacts were relatively minimal. A recovery of the number of EPT taxa collected after construction indicated that the benthic macroinvertebrate community may be recovering from impacts of highway construction. However, this study only addressed a period of 3 years before, 3 years during, and 4 years post construction. Inferences cannot be made concerning the long-term impacts of the highway, highway traffic, runoff, and other factors associated with highway use. Continual monitoring of the watershed is necessary to determine if the highway has a continual impact on stream habitat, water quality, and biotic integrity.     

Release of treated effluent into streams: A global review of ecological impacts with a consideration of its potential use for environmental flows

1. Worldwide, the addition of treated wastewater (i.e. effluent) to streams is becoming more common as urban populations grow and developing countries increase their use of wastewater treatment plants. Release of treated effluent can impair water quality and ecological communities, but also could help restore flow and maintain aquatic habitat in water-stressed regions. To assess this range of potential outcomes, we conducted a global review of studies from effluent-fed streams to examine the impacts of effluent on water quality and aquatic and riparian biota.
2. We identified 147 quantitative studies of effluent-fed streams, most of which were from the U.S.A. and Europe. Over 85% of the studies identified water quality as a primary study focus, including basic physical and chemical parameters, as well as trace organic contaminants. Nearly 60% of the studies had at least some focus on aquatic or riparian biota, primarily fish, aquatic invertebrates, and basal resources (e.g. algae).
3. Effluent inputs generally impaired water quality near discharge points, mainly through increased water temperature, nutrients, and concentrations of trace organic contaminants, but also via decreased dissolved oxygen levels. The majority of ecological studies found that basal resources, aquatic invertebrates, and fish were negatively affected in a variety of ways (e.g. biodiversity losses, replacement of sensitive with tolerant species). However, several studies showed the importance of effluent in providing environmental flows to streams that had been dewatered by anthropogenic water withdrawals, especially in semi-arid and arid regions.
4. Knowledge gaps identified include the abiotic impacts of effluent, such as changes in channel morphology and hydrology (e.g. how nutrient-rich and warmer effluent affects infiltration rates or interactions with groundwater), the effects of effluent on plants and vertebrates (e.g. amphibians, birds), and the impact of effluent-induced perennialisation on naturally intermittent or ephemeral streams.
5. Although effluent-fed streams often exhibit signs of ecological impairment, there is great potential for these systems to serve as refuges of aquatic biodiversity and corridors of ecological connectivity when wastewater treatment standards are high, especially in semi-arid and arid regions where natural streams have been dewatered.

     

Evaluation of water services system through LCA. A case study for Iasi City,Romania

Purpose

The main objective of this paper is to analyse through life cycle assessment (LCA), the entire water services system in Iasi City (Romania): a representative city for the problems faced by the water services sector in Romania. Furthermore, the study is aimed at demonstrating the usefulness of the LCA approach as a support instrument for water resources management.

Methods

The life cycle inventory (LCI) of the Iasi water system was organized considering the water system components, as well as their function related to the water use life cycle: before the tap system as production phase (water abstraction, transport, treatment and distribution) and after the tap section as post-use phase (wastewater collection, treatment and discharge). The foreground data describing the LCI processes were provided directly by the company operating the Iasi water system, while the data for the background processes were sourced or selected from Ecoinvent 2.0 database. The assessment considers the quantification of environmental impacts (according to the CML 2000 baseline and Ecological Scarcity 2006 methodologies) of water supply (abstraction, treatment and distribution) and wastewater disposal (collection and treatment) relative to 1 m³ of tap water.

Results and discussion

For this given system, the results have pointed out that the before the tap system generates higher impacts than the after tap system, mainly due to the energetic effort needed for water supply and the fairly high water losses in the distribution system. However, the after the tap system, specifically the discharge of treated wastewater is still responsible for many of the water-related impact such as Eutrophication (when using CML) or Emissions to surface waters (when using the Ecological Scarcity method). Apart from the LCA approach, this study presents several scenarios for the improvement of the environmental performance of the water services, such as: changing between water sources, improving the distribution system and upgrading the wastewater treatment plant.

Conclusions

This study has demonstrated the usefulness of LCA to describe, compare and predict the environmental performance of complex water services systems (and all its components). The results have provided a reference case for the environmental profile of Iasi city water system, and have enabled the identification of its improvement alternatives. Also, this study, which represents a premiere for Romania, has opened future research directions which may include the development perspectives of the Iasi water services system, as well as improvements of LCIA methodologies to better represent the local specific water-related impacts.     

乡土植物生活型构成对川渝地区边坡植被水土保持效益的影响

乡土植物灌木化建植是高速公路边坡防护的重要生态模式。为了探讨生态恢复过程中乡土植物的生活型构成对边坡植被水土保持效益的影响,揭示乡土植物生活型-物种多样性-生态系统功能间的偶合关系,借助3个物种配置试验,于2009年4月构建了以草本、灌木或乔木为主体的草本型、灌木型、乔木型绿化配置及草-灌-乔混合型试验区。自建植次年(2010年)起,对试验区进行持续5a生态监测。结果表明:1)边坡植被的物种丰富度与乡土植物的生活型有关,呈现乔木型灌木型草-灌-乔混合型草本型趋势;2)植被的物种多样性(Shannon-Wiener指数、Pielou指数)与乡土植物生活型构成及建植年限有关:建植后第1、第2年,多样性水平呈草本型草-灌-乔混合型灌木型乔木型变化趋势;自建植后第三起(2012—2014年),呈草-灌-乔混合型草本型灌木型乔木型波动;3)植被的水土保持性能(径流系数、侵蚀模数)与群落的物种多样性密切相关:多样性水平越高,水土保持性能越强。可见,乡土植物的生活型构成对提高边坡植被的物种多样性、改善生态性能至关重要。     

Life Cycle Environmental and Cost Impacts of Using an Algal Turf Scrubber to Treat Dairy Wastewater

Using algae to simultaneously treat wastewater and produce energy products has potential environmental and economic benefits. This study evaluates the life cycle energy, greenhouse gas (GHG) emissions, eutrophication potential, and cost impacts of incorporating an algal turf scrubber (ATS) into a treatment process for dairy wastewater. A life cycle inventory and cost model was developed to simulate an ATS treatment system where harvested algae would be used to generate biogas for process heat and electricity generation. Modeling results show that using an ATS significantly reduces eutrophication impacts by reducing chemical oxygen demand, nitrogen, and phosphorus in the wastewater. With low water recirculation rates through the ATS and high algae productivity, inclusion of the ATS results in net energy displacement and a reduction of GHG emissions compared to a system with no ATS. However, if high water recirculation rates are used or if algae biosolids from the digester are dried, the system results in a net increase in energy consumption and GHG emissions. The life cycle treatment cost was estimated to be $1.42 USD per cubic meter of treated wastewater. At this cost, using an ATS would only be cost effective for dairies if they received monetary credits for improved water quality on the order of $3.83 per kilogram of nitrogen and $9.57 per kilogram of phosphorus through, for example, nutrient trading programs.     

城市土壤质量演变及其生态环境效应

城市化是人类活动影响下自然生态系统向人工生态系统的急剧变化形式,它引起一系列生态环境条件的改变。城市对土壤资源的数量和质量产生深刻的影响,包括土壤地表封闭引起的土壤生态功能彻底消失,土壤物理性质恶化,土壤形态学特征和演变过程深受人为作用的影响。短程变异增加而总体多样性降低,土壤污染加剧,土壤养分富集等多方面,城市过程中土地利用变化对土壤产生一系列生态环境效应,如养分径流输出增加,热缓冲能力下降,污染转移危险性上升等,有必要迅速开展城市化过程中土壤质量演变及其生态环境效应的系统研究,为我国城市可持续发展提供理论依据。     

Nutrient status and retention in pristine and disturbed wetlands in Uganda: management implications

Wetlands in Uganda experience different forms of human pressure ranging from drainage for agriculture and industrial development to over harvesting of wetland products. In order to develop sustainable management tools for wetland ecosystems in Uganda and the Lake Victoria Region, water quality analyses were carried out in a rural undisturbed (pristine) wetland (Nabugabo wetland in Masaka) and two urban wetlands that are experiencing human and urban development pressure (the Nakivubo wetland in Kampala and Kirinya wetland in Jinja). The former wetland forms the main inflow into Lake Nabugabo while the other two border the northern shore of Lake Victoria, Uganda. Nabugabo wetland buffers Lake Nabugabo against surface runoff from the catchment, while Nakivubo and Kirinya wetlands provides a water treatment function for wastewater from Kampala City and Jinja town respectively, in addition to buffering Lake Victoria against surface runoff. Water quality was assessed in all the wetland sites, and in addition nutrient content and storage was investigated in the main plant species (papyrus, Phragmites, Miscanthidium and cocoyam) in Nakivubo and Kirinya wetlands. A pilot experiment was also carried out to assess the wastewater treatment potential of both the papyrus vegetation and an important agricultural crop Colocasia esculenta (cocoyam). Low electrical conductivity, ammonium–nitrogen and ortho-phosphate concentrations were recorded at the inflow into Nabugabo wetland (41.5 μS/cm; 0.91 mg/l and 0.42 mg/l respectively) compared to the Nakivubo and Kirinya wetlands (335 μS/cm; 31.68 mg/l and 2.83 mg/l and 502 μS/cm; 10 mg/l and 1.87 mg/l respectively). The papyrus vegetation had higher biomass in Nakivubo and Kirinya wetlands (6.7 kg DW m⁻²; 7.2 kg DW m⁻² respectively), followed by Phragmites (6.5, 6.7), cocoyams (6.4, 6.6) and Miscanthidium (4.0, 4.2). The papyrus vegetation also exhibited a higher wastewater treatment potential than the agricultural crop (cocoyam) during the pilot experiment (maximum removal degree of ammonium–nitrogen being 95% and 67% for papyrus and yams). It was concluded that urbanisation pressure reduces natural wetland functioning either through the discharge of wastewater effluent or the degradation of natural wetland vegetation. It is recommended that wetland vegetation be restored to enhance wetland ecosystem functioning and for wetlands that are not yet under agricultural pressure, efforts should be made to halt any future encroachment.     

Life cycle assessment of cheese and whey production in the USA

Purpose

A life cycle assessment was conducted to determine a baseline for environmental impacts of cheddar and mozzarella cheese consumption. Product loss/waste, as well as consumer transport and storage, is included. The study scope was from cradle-to-grave with particular emphasis on unit operations under the control of typical cheese-processing plants.

Methods

SimaPro© 7.3 (PRé Consultants, The Netherlands, 2013) was used as the primary modeling software. The ecoinvent life cycle inventory database was used for background unit processes (Frischknecht and Rebitzer, J Cleaner Prod 13(13–14):1337–1343, 2005), modified to incorporate US electricity (EarthShift 2012). Operational data was collected from 17 cheese-manufacturing plants representing 24 % of mozzarella production and 38 % of cheddar production in the USA. Incoming raw milk, cream, or dry milk solids were allocated to coproducts by mass of milk solids. Plant-level engineering assessments of allocation fractions were adopted for major inputs such as electricity, natural gas, and chemicals. Revenue-based allocation was applied for the remaining in-plant processes.

Results and discussion

Greenhouse gas (GHG) emissions are of significant interest. For cheddar, as sold at retail (63.2 % milk solids), the carbon footprint using the IPCC 2007 factors is 8.60 kg CO₂e/kg cheese consumed with a 95 % confidence interval (CI) of 5.86–12.2 kg CO₂e/kg. For mozzarella, as sold at retail (51.4 % milk solids), the carbon footprint is 7.28 kg CO₂e/kg mozzarella consumed, with a 95 % CI of 5.13–9.89 kg CO₂e/kg. Normalization of the results based on the IMPACT 2002+ life cycle impact assessment (LCIA) framework suggests that nutrient emissions from both the farm and manufacturing facility wastewater treatment represent the most significant relative impacts across multiple environmental midpoint indicators. Raw milk is the major contributor to most impact categories; thus, efforts to reduce milk/cheese loss across the supply chain are important.

Conclusions

On-farm mitigation efforts around enteric methane, manure management, phosphorus and nitrogen runoff, and pesticides used on crops and livestock can also significantly reduce impacts. Water-related impacts such as depletion and eutrophication can be considered resource management issues—specifically of water quantity and nutrients. Thus, all opportunities for water conservation should be evaluated, and cheese manufacturers, while not having direct control over crop irrigation, the largest water consumption activity, can investigate the water use efficiency of the milk they procure. The regionalized normalization, based on annual US per capita cheese consumption, showed that eutrophication represents the largest relative impact driven by phosphorus runoff from agricultural fields and emissions associated with whey-processing wastewater. Therefore, incorporating best practices around phosphorous and nitrogen management could yield improvements.     

Potential for Plant-Based Remediation of Pesticide-Contaminated Soil and Water using Nontarget Plants such as Trees,Shrubs, and Grasses

Appropriate environmental management of pesticides includes their proper application, use of filter strips and riparian buffers to contain pesticides in runoff from fields, prompt cleanup of spills, and treatment processes for wastewater associated with manufacturing and equipment usage. Plants have beneficial effects in the management of pesticide-contaminated soil and water, including direct metabolism of many pesticides, stimulation of microbial activity in the root zone, extraction of contaminated water, and reduction of infiltrating contaminated water. In this work, we review the literature on nontarget plants that can grow in pesticide-contaminated soil and water, and the fate of pesticides in filter strips, riparian buffers, and vegetated remediation environments. Past research indicates that there are significant differences in the tolerance of plants to pesticides present in soil and water, and that some plants are more effective than others in the remediation of pesticide-contaminated soil and water. Thus, there is value in the identification of tolerant plants and favorable plant-based remediation technologies for management of pesticides and contaminated sites.