Impact of boar exposure on puberty attainment and breeding outcomes in gilts

We examined the most effective method of boar exposure for the attainment of puberty in 89 gilts. At 160 days of age, we allocated gilts to daily direct contact with a vasectomized boar after movement of pen groups of gilts to a detection-mating area (DGB: n = 30); daily direct contact with boars in the gilt home pens (DBG: n = 31); or daily fenceline contact between boars and gilts housed in individual gilt stalls (FBG: n = 28). DGB gilts were younger (P < or = 0.05) than FBG gilts at puberty. Direct boar contact reduced the interval from initial boar contact to puberty in DGB and DBG gilts, compared to fenceline contact in FBG gilts (P < 0.05). There was no difference (P > or = 0.05) between treatment for pubertal weight, backfat, lifetime growth rate, or duration of first pubertal estrus. Backfat depth and leptin concentration at 160 days of age were positively correlated (P < or = 0.05). We detected no relationships between leptin or IGF-1 concentration at 160 days of age and the interval from initial exposure to a vasectomized boar to puberty (P > 0.05). Based on objective criteria, fenceline contact with a boar (BC) during artificial insemination improved the quality of artificial insemination compared to no boar contact (NC) (P < 0.05).     

A note on the effects of contact frequency and time of day of boar exposure on the efficacy of the boar effect

Sixty-four Large White/Landrace crossbred gilts were used in this study, 16 gilts being allocated to each of four treatments to compare the effects on puberty attainment of exposure to boar contact either 0, 1 or 2 times daily. The once-daily exposure occurred in either the morning or the afternoon (AM vs. PM). Treatments were of 20-min duration starting at a mean gilt age of 160 days and continuing for 60 days. Boar exposure significantly increased the proportion of gilts attaining puberty within 60 days of the commencement of treatments (P < 0.05) compared with gilts not receiving boar contact. Gilts receiving boar exposure twice daily attained puberty significantly earlier than did gilts in the two treatment groups (AM and PM, respectively) given a single daily boar exposure period (mean gilt ages at puberty 176.4 vs. 192.7 and 189.2 days of age, respectively, P < 0.05). It is concluded that (a) twice-daily boar contact enhances the efficacy of the boar effect in gilts above that seen with a single daily boar exposure period and (b) this enhanced response of the gilt is due to the frequency of boar contact and not to the time of day at which the contact occurs.     

The effect of early contact with mature boars on reproductive efficiency in the gilt

The effect of first contact of gilts with a mature boar at 23 or 28 weeks of age on their subsequent reproductive efficiency was studied over a 12-month period at a large intensive piggery in southern Australia. Following this contact, the gilts entered the mating shed at 29 weeks of age and were checked daily for oestrus, as assessed by the back-pressure test in the presence of the boar. Gilts that showed moderate or high responses were taken to a boar for mating. Sexual receptivity was then assessed by the time taken to “stand” after the first mount by the boar. Gilts that remained unmated at 35 weeks of age were culled, and their ovaries were examined.Of the 2660 gilts in the study, 2349 were mated and they had a farrowing rate of 88.2% with a mean litter size of 9.5 piglets, of which 0.7 piglets (7.4%) were born dead. The reproductive efficiency of the gilts following earlier contact with the boar was consistently higher than that of gilts exposed later. The mating rate of the week 23 gilts was greater than that of the week 28 gilts (70.1 vs 66.0%, P < 0.01), more appeared to show a high level of sexual receptivity (97.0 and 94.6%, N.S.) and fewer failed to mate when put to a boar (6.1 vs 9.5%, P < 0.01). The percentage of prepubertal gilts at 35 weeks of age was also lower (1.46 vs 3.03%, P < 0.01). The improved reproductive performance was estimated to be equivalent to 0.24 extra piglets born per gilt.     

Effectiveness of frozen boar semen under practical conditions of artificial insemination

A technique of boar semen deep-freezing and frozen semen use was tested in practice. 338 sows and 43 gilts belonging to small herds with less than 10 females each were inseminated without oestrus detection by a teaser boar. About 58 % of the inseminated females produced 9.3 piglets per litter. But there were differences between parities. The sows had the highest fertility rate, whereas the gilts showed a significantly lower farrowing rate (59.8% vs 41.9%; P < 0.05). The standing reaction of the female to the back pressure test made by the inseminator and the behaviour of the female during insemination had an effect on the farrowing rate. The best result was obtained after a standing reaction and a behaviour score of 1 (64.5% and 9.6 piglets for farrowing rate and litters size respectively). Farrowing rate for inseminators ranged from 44.3% to 62.4% among inseminators. Farrowing rate for females inseminated with frozen semen from Large-White, Landrace, Pietrain boars was not different, but there were significant differences between the boars. Results showed that insemination with deep-frozen boar semen could be used under practical conditions as an additional technique to the use of fresh semen.     

The effects of zearalenone on reproduction in swine. II. The effect on puberty attainment and postweaning rebreeding performance

Eighty-five prepuberal, crossbred gilts received, ad libitum, a diet containing 0 or 10 ppm purified zearalenone for 30 d beginning at 145 to 193 d of age. At the end of this period all gilts were placed on the control diet and exposed daily to a mature boar for 60 d. Within 3 to 5 d of zearalenone ingestion, gilts showed marked vulval swelling and reddening, which continued for the 30-d feeding period. Thereafter symptoms slowly subsided. Zearalenone treated gilts showed first estrus significantly later than controls (P < 0.05), but the proportion of gilts showing estrus within 60 d of boar exposure was similar (P > 0.05). The length of the first estrous cycle was not affected by the ingestion of zearalenone before puberty (P > 0.05). In a second study, 65 multiparous, crossbred sows were full-fed twice daily a ration containing 0 or 10 ppm of purified zearalenone beginning 14 d before weaning. Postweaning, all sows were fed the control diet, were checked for estrus daily, and inseminated at the first postweaning estrus. Neither sows nor gilts from their litters exhibited signs of hyperestrogenism during treatment. Weaning to estrus interval was significantly extended in zearalenone treated sows (P < 0.05), but all other variables of fertility assessed were similar. These data suggest that zearalenone ingestion before puberty delays the stimulation of puberty associated with boar exposure, but does not affect subsequent cyclicity if zearalenone is removed from the ration. Similarly, zearalenone ingestion during lactation delays the return to estrus after weaning, but does not affect subsequent fertility when removed from the ration at weaning.     

Effect of time of ovulation and sperm concentration on fertilization rate in gilts

In normal production practices, sows and gilts are inseminated at least twice during estrus because the timing of ovulation is variable relative to the onset of estrus. The objective of this study was to determine if a normal fertilization rate could be achieved with a single insemination of low sperm number given at a precise interval relative to ovulation. Gilts (n=59) were randomly assigned to one of three treatment groups: low dose (LD; one insemination, 0.5 x 10(9) spermatozoa), high dose (HD; one insemination, 3 x 10(9) spermatozoa) or multiple dose (MD; two inseminations, 3 x 10(9) spermatozoa per insemination). Twice daily estrus detection (06:00 and 18:00 h) was performed using fenceline boar contact and backpressure testing. Transrectal ultrasonography was performed every 6 h beginning at the detection of the onset of standing estrus and continuing until ovulation. Gilts in the LD and HD groups were inseminated 22 h after detection of estrus; MD gilts received inseminations at 10 and 22 h after detection of estrus. Inseminations were administered by using an insemination catheter and semen was deposited into the cervix. The uterus was flushed on Day 5 after the onset of estrus and the number of corpora lutea, oocytes, and embryos were counted. Time of insemination relative to ovulation was designated as 40 to >24 h, 24 to >12 h, and 12 to 0 h before ovulation and >0 h after ovulation. The LD gilts had fewer embryos (P<0.04), more unfertilized oocytes (P<0.05) and a lower fertilization rate (P<0.07) compared to MD gilts. The effects of time of insemination relative to ovulation and the treatment by time interaction were not significant. We conclude that a cervical insemination with low spermatozoa concentration may not result in acceptable fertility even when precisely timed relative to ovulation.     

In vitro development of embryos from superovulated gilts treated with the progesterone agonist, altrenogest (Regu-Mate) or the prostaglandin analogue, cloprostenol (Planate)

This study was conducted to compare the in vitro development of embryos from superovulated postpubertal gilts synchronized with progesterone agonist altrenogest (REG, Regu-Mate) and those from superovulated prepubertal gilts synchronized with prostaglandin analogue cloprostenol (PLA, Planate). Ten postpubertal gilts that had exhibited estrus at least once were fed 20 mg/d of REG from Day 0 (the first day of treatment, may have been any day of the estrous cycle) to Day 17. The gilts received intramuscularly (im) 1500 IU of equine chorionic gonadotropin (eCG) on the afternoon of Day 17, followed by 1000 IU of human chorionic gonadotropin (hCG) 84 h later. Eight prepubertal gilts received intramuscularly one dose of a combination of 400 IU of eCG and 200 IU of hCG (PG 600) on Day 0 (the first day of treatment), followed by 750 IU of hCG on Day 3. From Day 16 to Day 19, the prepubertal gilts received 350 mg/d of PLA, followed by 1500 IU of eCG on the afternoon of Day 19, then 1000 IU of hCG 84 h later. Gilts were checked for estrus with an intact boar. At estrus, all gilts were artificially inseminated and/or mated twice at 12-h intervals. Then 50 to 54 h after the hCG injection, a mid-ventral laparotomy was performed on each gilt. Corpora albicans (CA) and corpora hemorrhagica (CH) were counted, and oviducts were flushed in situ. The embryos recovered (1- to 2-cell) were cultured in modified Whitten's medium at 38.5 degrees C under an atmosphere of 5% CO2 in air for 144 h. The number of CA per gilt did not differ between the postpubertal and prepubertal gilts (11.9 vs 7.9, respectively; P > 0.05). However, the number of CH per gilt (27.5 vs 18.1, P = 0.05) and the number of embryos per gilt (26.2 vs 15.3, P < 0.05) were higher in postpubertal gilts than in prepubertal gilts. Furthermore, after 144 h of in vitro culture, the percentage of embryos cleaving to the >-16-cell (morula + blastocysts) or > or =32-cell (blastocysts) was greater (P < 0.05) in prepubertal gilts than in postpubertal gilts (85.2 vs 68.5, 55.7 vs 44.2, respectively). The total numbers of embryos examined were 122 and 260 in prepubertal and postpubertal gilts, respectively. These results show that postpubertal gilts treated with REG produced a higher number of embryos. However, better embryo development was noted with zygotes from prepubertal gilts primed with exogenous gonadotrophin, followed by synchronization with prostaglandin before induction of superovulation and insemination.     

Culling intervals and culling risks in four stages of the reproductive life of first service and reserviced female pigs in commercial herds

The objectives of this study were to measure culling intervals and culling risks in the four stages of the reproductive life of female pigs and to compare culling intervals between the number of services and between herd groups, based on herd productivity. We also compared survival patterns of females pigs between these herd groups. Our data set included lifetime records of 52,792 females born between 2001 and 2004 in 101 commercial herds. Two herd groups were selected on the basis of the upper 25th percentile of pigs weaned per mated female per 5 yr between 2002 and 2006, namely the high-performing herds, and ordinary herds. Culled females were also allocated into four groups based on the stages of their reproductive life when culled: unmated gilts, mated gilts, unmated sows, and mated sows. Culling intervals in unmated gilts and mated gilts were defined as the number of days from birth to culling and from first mating to culling, respectively. Culling intervals in unmated sows and mated sows were the number of days from weaning to culling. The number of services was categorized into two groups: first service and reservice groups. Multilevel linear mixed-effects models and survival analysis were performed. Culling intervals (±SEM) in unmated gilts, mated gilts, unmated sows, and mated sows were 302.9 ± 1.16, 98.4 ± 0.92, 14.3 ± 0.12, and 89.6 ± 0.42 d, respectively. Culling risks in the four groups were 5.6%, 7.1%, 58.0%, and 29.3%, respectively. In unmated gilts, mated gilts, and mated sows, the culling intervals in the high-performing herds were 43.0, 18.9, and 16.0 d shorter than those in ordinary herds, respectively (P < 0.05), but no difference was found between the herd groups for the culling interval of unmated sows. For mated sows in the reservice group, culling intervals of high-performing herds were ≥13.7 d shorter than those of the ordinary herds (P < 0.05), but for mated sows in the first service group, there was no difference in the culling interval between the herd groups. The culling hazard from 8 wk postweaning for mated sows in high-performing herds increased more rapidly than that in ordinary herds. In conclusion, to reduce culling intervals and improve herd productivity, we recommend implementing a strict culling policy for mated gilts and mated sows, especially reserviced females.     

Effect of prior FSH treatment on the estrus and ovulation responses to eCG in prepubertal gilts

The objective of this study was to determine the effect of pre-treatment of prepubertal gilts with FSH on the estrus and ovulatory responses to eCG injection at two ages. A total of 149 prepubertal Hypor gilts were selected at 150 days (n=76) or 180 days (n=73) of age and assigned to injection of 400 IU eCG plus 200 IU hCG (PG600), 600IU eCG alone (Folligon), pre-treatment with 72 mg FSH (Folltropin) administered as 6 x 12 mg injections at 12 h intervals with 600 IU Folligon 12h after last FSH injection, or non-injected controls. To facilitate detection of estrus, gilts were exposed to a mature boar for 15 min daily for 7 days. To determine ovulatory responses, blood samples were obtained on the day of injection and 10 days later and assayed for progesterone content. Following treatment at 150 days, one control gilt (5.3%) was deemed estrus but ovulation did not occur. Compared to treatment with Folligon alone, PG600 injection tended (P=0.1) to increase the estrus response (52.6% compared with. 26.3%) and increased (P<0.01) the ovulatory response (89.5% compared with. 47.4%). The estrous response in gilts pretreated with Folltropin was intermediate (42.1%) but the ovulatory response (47.4%) was the same as for Folligon alone. Following treatment at 180 days, two control gilts (10.5%) were deemed estrus and ovulation did occur in these gilts. There was no difference between hormone-treated groups for estrus or ovulatory responses, although the ovulatory response of PG600-treated gilts tended (P=0.1) to be greater than for the Folligon-treated group (89.5% compared with 66.7%), with Folltropin-pretreated gilts being intermediate (76.5%). These data demonstrate that the estrus and ovulatory responses of gilts were greater for PG600 than for Folligon and that while responses to PG600 were not affected by gilt age, for the combined Folligon groups, estrous response (P<0.02) and ovulatory response (P<0.05) improved with increased gilt age.     

Effects of exposure to an estrual female on the attainment of puberty in gilts

A total of 304 prepubertal gilts were randomly allocated to 4 treatment groups across 10 replications for a 50 d treatment period beginning at 170 d of age. The 4 treatment groups consisted of: 1) Gilts that were continuously exposed to one of a group of older, ovariectomized females that had been treated with 2 mg/ml estradiol benzoate to stimulate estrus (SE); 2) Gilts that were continuously exposed to an older, anestrous, ovariectomized female (OVX); 3) Gilts that were exposed to a mature boar for 15 min/d (BE); 4) Gilts that were isolated from any direct physical contact with other pigs (C). A gilt was considered to have attained puberty when she exhibited a standing reflex when mounted by the boar (BE group only) or to pressure applied manually to the back or had plasma progesterone concentrations > 2 ng/ml for 2 consecutive weeks. Data were analyzed as a randomized complete block design with treatment and replication in the model. A higher percentage of gilts attained puberty in the BE group than in the 3 other groups (52 vs 26, 30 and 32%, BE vs SE, OVX and C, respectively; P = 0.002). Gilts exposed to an estrual female or a mature boar attained puberty sooner after treatment was initiated than gilts in other treatment groups (12.6 and 17.8 vs 26.7 and 24.1 d, SE and BE vs OVX and C, respectively; P = 0.0003). Of the gilts attaining puberty during the experimental period, the highest percentage of gilts exhibited estrus within 10 d of treatment in the SE group (55.0 vs 26.1, 37.8 and 16.7%, BE vs SE, OVX and C, respectively; P = 0.05). Age at puberty was also lower SE or BE than OVX or C groups (176.3 and 181.0 vs 189.4 and 188.1 d, respectively; P = 0.0001). Weight at puberty was unaffected by treatment. These results suggest that exposure to an estrual female was effective in stimulating peripubertal females to express estrus, thus reducing the age at puberty. Boar exposure had a stimulatory effect not only at the initiation of exposure but throughout the experimental period, resulting in a higher percentage of gilts attaining puberty.     

Mixing gilts in early pregnancy does not affect embryo survival

There is general acceptance that mixing sows during the first 3 weeks of gestation is detrimental to embryo development and survival. However, there is a paucity of data describing the influence of group housing and remixing during the first 14 days of gestation on pregnancy outcomes. Using 96 purebred maternal (Large White)/terminal (Duroc) line gilts, the current study determined the effects of regrouping, and the timing of regrouping, during the pre-implantation period on embryo mortality. The study was conducted in 2 blocks, with 12 gilts allocated to each of 4 treatments in each block. At 175 days of age, the combination of PG600 and 20 min of daily physical boar contact was used to stimulate puberty, with boar contact resuming 12 days after first detection of oestrus and gilts receiving two artificial inseminations (AIs), 24 h apart, at their second oestrus. After their first AI gilts were allocated to one of four treatment groups (n=12 gilts/treatment). Gilts in one treatment group were housed individually in stalls (STALL). The remaining gilts continued to be housed in their pre-AI groups and were either not remixed (NOMIX), or remixed to form new groups on day 3/4 (RMIXD3/4) or day 8/9 (RMIXD8/9) of gestation (day 0=day of first detection of second oestrus and first insemination). Group-housed gilts were housed in groups of 6, with a space allowance of 2.4 m2/gilt. All gilts were fed once a day (2.2 kg/gilt). Reproductive tracts were collected on day 26.6+/-0.13 of gestation, and the number of corpora lutea (CL) and viable embryos counted. Pregnancy rate was similar across all treatments, averaging 94.5% across the four treatment groups. The number of embryos present on day 26 of gestation was unaffected by housing treatments (P>0.05); gilts in the STALL, NOMIX, RMIXD3/4 and RMIXD8/9 groups possessed 13.2+/-0.67, 12.9+/-0.66, 14.1+/-0.46 and 13.8+/-0.57 embryos, respectively. Similarly, embryo survival rates were 0.91+/-0.04, 0.85+/-0.04, 0.91+/-0.02 and 0.87+/-0.05 for the STALL, NOMIX, RMIXD3.4 and RMIXD8/9 groups, respectively (P>0.05). In conclusion, the current data indicate that individually housing gilts immediately after their first AI does not improve embryo survival. There also appear to be no adverse effects on embryo development or survival when group-housed, mated gilts are remixed during the first 10 days of gestation.     

Effect of social conditions during rearing on mating behaviour of gilts

The mating behaviour of 28 gilts was studied. The gilts were reared under two different social conditions known to affect both their puberty attainment and reproductive parameters during early pregnancy. The different social conditions were applied from an average age of 137 days onwards. Ten gilts were housed individually, having neither tactile nor visual contact with other pigs. The remaining gilts (n=18) were housed pairwise, having additional contact with gilts in adjacent pens and daily boar contact from 180 days of age onwards. At third oestrus, the gilts were artificially inseminated and subsequently introduced to one of three vasectomized boars for a period of 20 min. The gilts were slaughtered 10±1 days after insemination.

The mating behaviour varied considerably between individual gilts, partly because of differences in mating behaviour between the two groups of gilts. More (P<0.05) individually housed gilts showed a standing response latency upon introduction of the boar. During this latency period, the individually housed gilts initiated contact with the boar. Once the standing response was elicited, mating behaviour was similar in gilts of both social groups. One individually housed gilt did not show a standing response and consequently was not mated. The mating behaviour of the boars did not differ for the gilts of the two social conditions.

It was concluded that the social conditions of gilts during rearing affected their introductory sexual behaviour. The relationship with reproductive performance during early pregnancy is discussed.     

Boar culling and mortality in commercial swine breeding herds

The objectives of this study were to measure culling rate and mortality rate of boars; to compare boar life day (BLD: days from birth date to removal date), boar herd life day (BHLD: days from herd entry date to removal date) and herd entry age of boars between high-performing herds and ordinary herds (herd groups); to examine herd measurements for BLD, BHLD and boar age at herd entry; and to observe removal patterns and survival curves for boars by herd groups. This study used 2474 records of individual boars born in 108 herds from 2000 to 2003. Two herd groups were formed on the basis of the upper 25th percentile of pigs weaned per mated female per year (2001-2005). Mixed-effects models and survival analysis were performed. Means of BLD and BHLD (±S.E.M.) were 984 ± 9.5 and 781 ± 8.4 d, respectively. Annualized culling rate and mortality rate were 0.411 and 0.035 boars per 365 BHLD, respectively. Boars in high-performing herds had 51 higher BLD and 62 higher BHLD than those in ordinary herds (P < 0.01). High-performing herds had 32 d lower entry ages than ordinary herds (P < 0.01). Herd measurements such as herd mortality and herd size were not associated with both BLD and BHLD. The hazards in survival analysis were associated with herd groups (P < 0.05). Measurements in the present study provide benchmarks for boar removal in commercial herds.     

Steroidome and metabolome analysis in gilt saliva to identify potential biomarkers of boar effect receptivity

Optimal management of gilt reproduction requires oestrus synchronization. Hormonal treatments are used for this purpose, but there is a growing demand for non-hormonal alternatives, especially in organic farms. The boar effect is an important alternative opportunity to induce and synchronize oestrus without hormones. Before puberty, gilts exhibit a ‘waiting period’ during which boar exposure could induce and synchronize the first ovulation. We searched for salivary biomarkers of this period of boar effect receptivity to improve detection of the gilts to stimulate with the perspective of enhancing the efficacy of the boar effect. Saliva samples were collected from 30 Large-White × Landrace crossbred gilts between 140 and 175 days of age. Gilts were exposed twice a day to a boar and subjected to oestrus detection from 150 to 175 days of age. Among the 30 gilts, 10 were detected in oestrus 4 to 7 days after the first introduction of the boar and were considered receptive to the boar effect, 14 were detected in oestrus more than 8 days after first boar contact, and six did not show oestrus and were considered non-receptive. Saliva samples from six receptive and six non-receptive gilts were analyzed for steroidome and for metabolome using gas chromatography coupled to tandem mass spectrometry and ¹H nuclear magnetic resonance spectroscopy, respectively. Four saliva samples per gilt were analyzed: 25 days and 11 days before boar introduction, the day of boar introduction, 3 days later for receptive gilts or 7 days later for non-receptive gilts. Twenty-nine steroids and 31 metabolites were detected in gilt saliva. Salivary concentrations of six steroids and three metabolites were significantly different between receptive and non-receptive gilts: progesterone and glycolate 25 days before boar introduction, 3α5β20α- and 3β5α20β-hexahydroprogesterone, dehydroepiandrosterone, androstenediol, succinate, and butyrate 11 days before boar introduction, and 3β5α-tetrahydroprogesterone on the day of boar introduction. Thus, nine potential salivary biomarkers of boar effect receptivity were identified in our experimental conditions. Further studies with higher numbers of gilts and salivary sampling points are necessary to ascertain their reliability.     

The effects of PG600 and boar exposure on oestrus detection and potential litter size following mating at either the induced (pubertal) or second oestrus

Within gilt pools, incidences of delayed puberty attainment, failure to exhibit regular oestrous cycles and low first litter size are often high. Boar exposure is an effective method of accelerating puberty; however, the timing of gilt response can vary greatly. Although, PG600 (400 IU of PMSG and 200 IU of hCG; Intervet) can induce a rapid and synchronous ovulatory response, thus providing an alternative to boar contact, the quality of the response is often variable. This study compared the effect of PG600, either alone (NBC) or in conjunction with boar exposure (BC), on puberty attainment and maintenance of oestrous cyclicity. The effects of first mating these gilts at the hormonally induced (pubertal) or second oestrus on ovulation rate and early embryo survival were also studied. Eighty Large White cross terminal (Duroc) line gilts were used in this study. The study was conducted in two blocks, with 10 gilts allocated to each of the four treatments in each block. Gilts were artificially inseminated at the allocated oestrus, with the reproductive tracts collected at 26.5+/-0.29 days after first mating (mean+/-S.E.M.), and the number of corpora lutea and viable embryos recorded. Mean days-to-puberty was significantly reduced (P<0.05) when gilts received both PG600 and boar exposure as opposed to PG600 alone (5.7+/-0.15 versus 6.9+/-0.37 days; P<0.01). The proportion of gilts exhibiting an ovulatory response to PG600 was similar for the BC and NBC treatment groups (0.88 and 0.84); however, the proportion of gilts exhibiting visible signs of oestrus in response to PG600 was significantly higher for the BC compared to the NBC treatment groups (0.81 versus 0.49; P<0.05). Boar contact resulted in a numerical, but not significant, increase in the proportion of gilts exhibited a second oestrus (1.00 versus 0.76). There was no significant effect of boar contact on ovulation rate, embryo number or survival. Although ovulation rate was unaffected by oestrus at mating, embryo number was significantly increased (P<0.05) following mating at the second compared to the first oestrus (11.2+/-0.96 versus 7.8+/-1.17). In conclusion, the current data indicate that the timing of puberty attainment and oestrus detection are significantly improved when PG600 treated gilts receive full boar contact. Further, it is evident that mating gilts at their second as opposed to the hormonally induced oestrus significantly increases embryo number at day 26 post-mating.     

Effect of boar contact on follicular development and on estrus expression after weaning in primiparous sows

Boar contact can induce ovarian activity, advance estrus and stimulate estrous behavior in sows. High amounts of boar contact can, however, suppress estrous behaviour. The present study with primiparous sows was designed to compare sows that had contact with a teaser boar during detection of estrus, with sows that had no boar contact at all. Number of sows detected in estrus within 9 d after weaning, onset and duration of estrus, follicular dynamics and timing of ovulation were studied. Boar contact increased the number of sows that ovulated and showed estrus from 14 of 47 to 24 of 47 (P < 0.05). Average timing of ovulation was later for sows with boar contact (165 h vs 150 h after weaning). Duration of estrus, detected without a boar, was similar in the two groups. For the sows with boar contact, duration of estrus detected with a boar was longer than estrus detected without a boar (56 vs 38 h; P < .01). Follicular dynamics were not affected by boar contact; boar contact only increased the number of sows with ovulation. Ovulatory sows showed a larger increase in follicular diameter (P < 0.01) from weaning to Day 4 after weaning (from 2.3 to 5.4 mm) than anovulatory sows (from 2.5 to 4 mm). Anovulatory sows did not show follicular growth after Day 4. It is concluded that boar contact can increase the number of sows that ovulate and show estrus after weaning. Estrous behavior does not seem to be suppressed by contact with a teaser boar, compared to sows without boar contact.     

Oestrous behaviour in relation to fertility and fecundity of gilts

The reproductive performance of 2484 gilts was recorded over a 12-month period at a large intensive piggery in southern Australia. Between 29 and 35 weeks of age, gilts were examined daily for response to the back-pressure test (BPT). Those subjectively assessed as showing a moderate or high response were taken to a boar for mating. Gilts that had showed a moderate response to the BPT on their first day of mating had lower average litter size than gilts showing a high BPT response (9.05 and 9.35 piglets, respectively; P < 0.05). A quantitative assessment of sexual receptivity was made while the gilt was with the boar. Gilts that were mated while showing low sexual receptivity on the first day of mating had poorer farrowing rate (78.1 vs. 83.1%, respectively; P < 0.05), as well as lower litter size (9.03 vs. 9.35 piglets, respectively; P < 0.05) than gilts that were mated when showing high sexual receptivity. A moderate BPT response was followed by either low sexual receptivity or failure to mate on 81.4% of occasions, compared with 12.8% for a high BPT response. Since 75% of gilts that showed a moderate BPT response on the first day of mating showed a high response on the following day, it was concluded that mating should only be attempted when gilts exhibit a high response to the BPT. The mean farrowing rate and litter size for gilts at their first farrowing was 82.4% and 9.31 piglets (8.62 alive and 0.69 dead), respectively.     

The influence of male contact on plasma cortisol concentrations in the prepubertal gilt

Large White X (Large White X Landrace) prepubertal gilts, 165 days of age, were fitted with indwelling venous catheters and housed in modified metabolism crates. After a period of acclimatization, frequent blood samples were taken at regular intervals before, during and after the 7 gilts were exposed to various degrees of contact with male pigs. The plasma samples were assayed for cortisol concentration using a competitive protein-binding radioassay. Significantly elevated concentrations of plasma cortisol (P less than 0.001) occurred only when full physical contact between the boar and the gilts was allowed. Boar exposure without full physical contact induced only minor changes in plasma cortisol concentrations of gilts. Plasma cortisol concentrations have been shown to constitute a reliable indicator of a stress response in pigs, and so the results of this study suggest that tactile stimulation from a male pig induces a stress response in the recipient prepubertal gilt. This stress response in the gilt may be involved in the stimulation of puberty onset by contact with a mature boar (i.e. the 'boar effect').     

The involvement of boar submaxillary salivary gland secretions in boar-induced precocious puberty attainment in the gilt

The involvement of secretions from boar submaxillary salivary glands in mediating the induction of precocious puberty in the gilt was investigated as follows. Forty-eight Large White × (Large White × Landrace) prepubertal gilts from 12 litters were randomly allocated within litters by weight, to four treatment groups of six, in two replicates, at 145 days of age. Treatments commencing at a mean group age of 165 days, were: (1) control (no boar exposure); (2) gilts exposed to a mature sialectomised boar (submaxillary salivary glands were removed at 9 weeks of age); (3) gilts exposed to a mature sham-operated boar; (4) gilts exposed to a mature unoperated boar.Boar exposure occurred for 30 min per day for 75 days, or until pubertal oestrus was observed. Gilts showing pubertal oestrus were removed and slaughtered. Ovaries were examined to confirm reproductive status. Gilts failing to exhibit oestrus by 240 days of age were slaughtered and nominally ascribed a pubertal age of 245 days. Age at puberty was significantly earlier in all three boar-exposed treatments than in the control treatment (P<0.05 for treatments 2 and 3, P<0.001 for treatment 4; median ages at puberty being 227.0, 203.5 , 202.0 and 179.0 days for treatments 1 to 4 respectively). No frothy saliva was ever produced by the sialectomised boar, and chromatographic analysis of saliva produced by the sham-operated boar during mating revealed very low levels of 16-androstene pheromones, while levels in the unoperated boar's saliva were normal. These results provide further evidence for an important role of boar salivary pheromones in the induction of precocious puberty attainment in the gilt.