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M. Garayoa A. C. Villaro U. Klein B. Zimmermann L. M. Montuenga P. Sesma 《Cell and tissue research》1995,282(2):343-350
The presence of a vacuolar-type ATPase in Malpighian tubules of the ant Formica polyctena was investigated immunocytochemically, using antibodies to vacuolar ATPases of Manduca sexta midgut and bovine kidney. Specific labelling was observed at the brush border of the epithelium extending along the entire length of the tubules. These findings agree with the current view that a vacuolar ATPase is situated at the apical membrane of Malpighian tubule cells and other insect epithelial cells, being the energizing element of an electrogenic potassium pump. When antibodies were tested on tubules in different secretion conditions prior to fixation, no differences were observed in the distribution of the vacuolar ATPase.This work was supported by grants from the European Community (SCI-CT90-0480), from the Ministerio de Educación y Ciencia DGICYT, Spain (CE 91-0002), and from the Deutsche Forschungsgemeinschaft (Wi 698-3). 相似文献
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We have shown in an initial animal study that omentum will adequately vascularize a skin flap and allow transfer of this tissue composite for use in surgical reconstruction of the breast. Based on this experimental procedure, a technique employing a two-stage operation has been developed and used in 21 female patients in reconstruction of the breast after radical mastectomy. In the first stage, the omentum, attached to one gastroepiploic artery and vein, is exteriorized to the subcutaneous tissue of the lower abdominal wall. In the second stage, the distal omentum, now vascularizing the overlying skin and soft tissue, is moved as a secondary island flap to the anterior chest wall to complete the breast reconstruction. In all but 1 of our 21 patients who have been followed for 1 to 8 years, reconstruction of large defects, including the chest wall, breast mound, and infraclavicular axillary fold depression, was performed without use of a prosthesis. In one patient, there was complete necrosis of the flap due to vascular impairment; there were three instances of delayed healing and a significant but partial loss of the flap in one patient. All complications were encountered in the first 10 patients of the series during the time the technique was being refined. 相似文献
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B- and T-cell markers were studied in a patient with prolymphocytic leukemia, a rare variant of chronic lymphocytic leukemia. Thymus-derived features were identified on the membrane of the neoplastic lymphocytes using the following cellsurface markers: Heterologous T-cell antigen, sheep erythrocyte receptor, surface immunoglobulin, complement receptor, Fc receptor and mouse erythrocyte receptor. Cytogenetic studies of leukemic cells from unstimulated and mitogen-stimulated cultures revealed a consistent karyotype characterized by marker chromosomes and a decreased chromosome number, whereas chromosomal analysis of hair root cells yielded a normal karyotype. A uniform expression of T-cell antigens measured on single leukemic cells by quantitative microphotometric immunoautoradiography correlated with the cytogenetic findings which are compatible with a descent from one progenitor cell. 相似文献
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S S Kroll 《Plastic and reconstructive surgery》1999,104(3):687-693
A new method for nipple reconstruction is described that combines revision of an autologous tissue breast mound with creation of a projecting nipple. The method is applicable only to reconstructed breast mounds that must be reduced or lifted to achieve symmetry with the opposite breast. In this technique, the mound is reduced as if it were a normal breast, using an inverted-T or vertical mammaplasty pattern. In this way, breast projection can be increased and, if necessary, the inframammary fold can be elevated. A rectangular flap is created from skin and subcutaneous tissue that would normally be discarded during the breast reduction, and this flap is wrapped around on itself to form a projecting nipple. This new technique avoids the flattening of the breast mound usually seen after nipple reconstruction because it does not take tissue away from the completed breast mound to make the nipple. In appropriate patients who require reduction in size of their reconstructed breast mound, the wraparound flap nipple reconstruction is worth considering. 相似文献
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Possible cooperation of differential adhesion and chemotaxis in mound formation of Dictyostelium.
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In the mound stage of Dictyostelium discoideum, pre-stalk cells sort and form a tip at the apex. How this pattern forms is as yet unknown. A cellular level model allows us to simulate both differential cell adhesion and chemotaxis, to show that with differential adhesion only, pre-stalk cells move to the surface of the mound but form no tip. With chemotaxis driven by an outgoing circular wave only, a tip forms but contains both pre-stalk and pre-spore cells. Only for a narrow range of relative strengths between differential adhesion and chemotaxis can both mechanisms work in concert to form a tip containing only pre-stalk cells. The simulations provide a method to determine the processes necessary for patterning and suggest a series of further experiments. 相似文献
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Male Australian brush-turkeys, Alectura lalhami, in southeastQueensland, Australia, exhibited five different patterns ofincubation mound ownership. These related to the process ofmound acquisiton (whether by construction or usurpation) andthe number of mounds (one or two) maintained by males per breedingseason. Mound ownership appeared to be a prerequisite for matingsuccess. A common alternative mating tactic exhibited by numerousmoundless males involved interloping on the mounds of othermales; none of these were successful in obtaining copulations.The males most successful in receiving visits from laying femaleswere those maintaining two mounds: these males received a meanof 34 eggs, compared with 18 eggs received by males buildingone mound. Males that rebuilt after initial expulsion receivedeight eggs per season. Males appeared able to maximize theirmating success by reducing the number of alternative incubationsites by expelling other males, and to minimize the extremeenergetic costs of mound construction by usurping establishedmounds. 相似文献
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J S Turner 《Physiological and biochemical zoology : PBZ》2001,74(6):798-822
Patterns and rates of air movements in the mounds and nests of Macrotermes michaelseni were studied using tracer methods. Wind is a significant source of energy for powering nest ventilation, despite the mound being a completely enclosed structure. Nests are ventilated by a tidal movement of air driven by temporal variation in wind speed and wind direction. Density gradients sufficiently steep to drive bulk flow by natural convection will be rare. However, metabolism-induced buoyant forces may interact with wind energy in a way that promotes homeostasis of the mound atmosphere. 相似文献
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The arbuscular mycorrhizal (AM) status of two plant communities on a calamine spoil mound (rich in cadmium, lead and zinc)
in southern Poland was surveyed: an undisturbed grassland community and an early succession community that developed after
complete removal of the surface layer of the calamine substrate about 10 years earlier. The undisturbed site harbored 40 herbaceous
species making up 87% of the absolute cover. AM colonization was recorded in 25 species accounting for 77% of the relative
cover. Species with 51–75% AM root colonization such as Festuca ovina and Leontodon hispidus dominated the undisturbed turf, contributing 45% to the relative cover. Carex ssp. were the most abundant nonmycorrhizal plants and accounted for 9% of the relative cover. Spores of Glomus aggregatum, G. constrictum, G. fasciculatum, G. pansihalos, Glomus sp. and Entrophospora sp. averaged 25 per 100 g dry substrate at the undisturbed site. The disturbed site was colonized by 25 species accounting
for 17% of the absolute cover. Among the AM plants, most abundant were the species with up to 20% AM root colonization, such
as Agrostis stolonifera and Thymus pulegioides, wich accounted for 24% of the relative cover. Nonmycorrhizal species, such as Biscutella laevigata,
Cardaminopsis arenosa, Gypsophila fastigiata and Silene vulgaris, dominated the early succession community and contributed 64% to the relative cover. Spores of G. fasciculatum and Entrophospora sp. averaged 20 per 100 g dry substrate at the disturbed site.
Accepted: 29 November 1996 相似文献
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Microorganisms that were isolated from steppe soils buried below kurgans from 5800 to 750 years ago were analyzed for the completeness of isolation, total biomass (the sum of glucose-reactivated and resting microbial cells), and active biomass (metabolically active cells). The metabolic state of microbial communities in buried and modern background soils was estimated from the proportion of active and total biomasses. The paleosoils were found to be characterized by lower total and active biomasses and a lower proportion of active microorganisms as compared to the modern background soils. The age-dependent decrease in the content of active microorganisms in the microbial communities of palesoils was not monotonic. For instance, the 4000-year-old paleosoil was characterized by a high total biomass and a relatively low content of active microorganisms, whereas the 1950-year-old paleosoil was characterized by a relatively low total biomass and a relatively high content of active microorganisms. This could reflect the temporal dynamics of paleoclimatic conditions in the geographic region under study. 相似文献
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The “shell mound” Indians exhibit both sexual and bilateral variations in comparative long bone lengths. Mean long bone lengths are shortest in the Indian group when compared to those reported for both Caucasians and Negroes. The radiohumeral and humero-femoral indices of the “shell mound” Indians are higher than those reported for either Caucasians or Negroes. There is a relatively high degree of anterior-posterior curvature of the femur in the Indian group when compared to the femora of Caucasian and Negroes. There is apparently no correlation between maximum femoral length and trochanter length in comparing right and left sides in either sex. The collo-diaphyseal angles are greater on the average on the left side in both sexes. The femora of male “shell mound” Indians exhibits greater average collo-diaphyseal angles than do the femora of male Caucasians. 相似文献
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Katell G. Hamon Stewart D. Frusher L. Richard Little Olivier Thébaud André E. Punt 《Reviews in Fish Biology and Fisheries》2014,24(2):577-592
The rock lobster, Jasus edwardsii, lies on a global “hotspot” for climate change in the southeastern Australian state of Tasmania. The short-term effects of climate change are predicted to lead to an increasing exploitable biomass in the south and declining biomass in the north of the state. The future of the fishery is highly uncertain due to climate change, but also due to insecurities linked to the market conditions. The market for Tasmanian rock lobster is driven by the demand of a single market, China, which absorbs 75 % of the catch. This study examines how fishers can adapt to external perturbations that affect the social and economic viability of the fleet and the ecological dynamics of the stock. Three fleet dynamic models of increasing complexity are used to investigate the effects of climate change and lobster price changes on the fishery. There could be local depletion leading to negative short-term profit for the fleet if it is static and the proportion of the total catch taken in each region of the fishery does not respond to climate-induced-changes. Better outcomes would occur if the fleet adapts dynamically to environmental conditions, and fishing effort follows stock abundance, which would counter-act the short-term effects of climate change. Only a model with explicit representation of economic drivers can fully capture the local economic and social impacts of large scale global perturbations. 相似文献
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RIITTA SAVOLAINEN 《Ecological Entomology》1990,15(1):79-85
Abstract. 1. Twenty-three nests of the submissive ant Formica fusca L. were sampled in two adjacent territories of the dominant wood ant Formica polyctena Först. The nests were dug up at different distances from the wood-ant mounds. Distance is assumed to be inversely related to the extent of disturbance of F. fusca by F. polyctena.
2. Colony success of F. fusca was assayed by counting the numbers of workers (colony size), and worker and sexual offspring in a nest. For individual size, the head width and dry weight of fifty workers per nest were measured.
3. Colony size correlated positively with the numbers of worker and sexual offspring in one territory, but only with worker offspring in the other.
4. Distance from the wood-ant mound correlated positively with colony size and numbers of worker and female offspring in one territory. In the other territory distance correlated positively with number of females, but negatively with number of worker offspring. No significant correlations between distance and the size measurements were obtained.
5. Because of its nonaggressive behaviour F. fusca may nest fairly close to a wood-ant mound but is likely to show reduced abundances in terms of nest density and forager number, and, ultimately, lowered fitness. 相似文献
2. Colony success of F. fusca was assayed by counting the numbers of workers (colony size), and worker and sexual offspring in a nest. For individual size, the head width and dry weight of fifty workers per nest were measured.
3. Colony size correlated positively with the numbers of worker and sexual offspring in one territory, but only with worker offspring in the other.
4. Distance from the wood-ant mound correlated positively with colony size and numbers of worker and female offspring in one territory. In the other territory distance correlated positively with number of females, but negatively with number of worker offspring. No significant correlations between distance and the size measurements were obtained.
5. Because of its nonaggressive behaviour F. fusca may nest fairly close to a wood-ant mound but is likely to show reduced abundances in terms of nest density and forager number, and, ultimately, lowered fitness. 相似文献