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1.
Identification of cholinoreceptors (CR) of the soma of neurons RPa3 and LPa3 of the snail is performed using selective cholinomimetics and cholinolytics during the recording of transmembrane ionic currents. Agonists of the nicotinic (NCR) and muscarinic (MCR) types of cholinolytics evoked a brief activation of the receptors, with the exception of carbamylcholine, followed by an irreversible blocking. All selective cholinomimetics bonded with the same membrane centers which acetylcholine (AC) activated. The nicotinic and muscarinic cholinolytics decreased the amplitude of the input current elicited by AC; however, the use of scopolamine and platyphylline was without effect. It is speculated that the soma of neurons RPa3 and LPa3 exhibits NCR and MCR which have a number of pharmacological features distinguishing them from the corresponding CR of vertebrates. The MCR of these neurons must be classed as a special subtype differing from the well-known M1 and M2 subtypes.M. V. Lomonosov State University of Moscow. Translated from Neirofiziologiya, Vol. 24, No. 1, pp. 77–86, January–February, 1992.  相似文献   

2.
Research was carried out into the ionic aspects of depolarization potentials produced inHelix lucorum neuron RPa4 by injecting three cholinomimetics into the soma: acetylcholine, nicotine, and muscarine. Substances were used suppressing Na+, K+, Ca2+, and Cl conductance at the membrane. Acetylcholine brought about increased Na+, Ca2+; and Cl conductance. As the choline component was only slight, due to the similarity of membrane and resting potential for chloride, it might be deduced that the prevailing response to acetylcholine is associated with chemically controlled input of Na+ and Ca2+ into the cell. Nicotine and muscarine induced mainly sodium and calcium conductance respectively.M. V. Lomonosov State University, Moscow. Translated from Neirofiziologiya, Vol. 21, No. 3, pp. 305–314, May–June, 1989.  相似文献   

3.
Acetylcholine, nicotine, a selective agonist of N-cholinoreceptors suberildicholine dibromide, as well as a selective agonist of M-cholinoreceptors 5-methylfurmethide inhibited spike discharges in a dose-dependent manner up to a complete ceasing of the firing in cholinoreceptors situated on the identified neurone TAN of African giant snail Achatina fulica. M-cholinoblocker metamizylum completely prevented the inhibitory effect of methylfurmethide. Central cholinoblocker aetherophen completely prevented the inhibitory effect of suberildicholine dibromide. Metamizylum or aetherophen used alone were only able to decrease the inhibitory effect of acetylcholine, whereas a mixture of these agents suppressed completely the acetylcholine-induced inhibition. The findings suggest that, on the TAN membrane, nicotinic and muscarinic cholinoreceptors co-exist and function in one and the same direction.  相似文献   

4.
Pharmacological division of the snail cholinoreceptors population of the identified neurone RPa4 by cholinoblockators of muscarine (atropine, platyphylline) and nicotine (d-tubocurarine) receptors allowed to reveal differences in the dynamics of reversible reduction of sensitivity of these receptors during their habituation to repeated iontophoretic acetylcholine applications. Maximum desensitization of nicotine receptors is weaker, develops slower and is eliminated faster after the end of rhythmic acetylcholine applications. An assumption is made that a more rapid and deeper lowering of muscarine cholinoreceptors sensitivity is due to an increase of intracellular concentration of free calcium at their activation by the agonist.  相似文献   

5.
Intracellular recordings have been made from salivary gland cells of the pond snail Planorbis corneus. Gland cells produced a dose-dependent biphasic response to the bath application of acetylcholine (ACh), an initial depolarization being followed by a hyperpolarization. Nicotine and the nicotinic agonist tetramethylammonium had an excitatory action on the gland cells. The muscarinic agonists acetyl-beta-methyl choline and arecoline were also stimulants, but muscarine, bethanechol and pilocarpine produced no response from gland cells at 10(-3) M. A number of cholinergic antagonists, including atropine, hexamethonium and curare, effectively blocked the response to ACh. The depolarizing phase of the ACh response resulted from an increased membrane permeability to Na+ ions, though the participation of other ionic species cannot be ruled out. The hyperpolarizing phase of the ACh response was produced by the activity of an electrogenic Na+/K+ pump.  相似文献   

6.
Leucine (leu)-enkephalin depresses or inhibits the peristaltic reflex of the isolated guinea-pig ileum. Opiate antagonists (naloxone and nalorphine), choline esters (acetylcholine, methacholine and carbachol), cholinomimetics (muscarine and arecoline) and polypeptides which stimulate peristalsis (eledoisin and angiotensin) antagonize the peristaltic block caused by leu-enkephalin. On the other hand, nicotinic ganglionic stimulants (nicotine and dimethylphenylpiperazine) as well as muscarinic ganglionic stimulants (McN-A-343 and AHR-602) do not restore the peristaltic reflex abolished by leu-enkephalin. Thus the inhibitory effect of leu-enkephalin is due mainly to an action on myenteric ganglia as well as on axon terminals of the myenteric plexus subserving the peristaltic reflex. The inhibitory action of leu-enkephalin may be ascribed to the opiate as well as to the cholinoceptive sites in the nervous elements in the myenteric plexus. The blocking action of leu-enkephalin is not associated with ganglionic muscarinic M-1 receptors as well as with ganglionic nicotinic receptors in the myenteric plexus of the guinea-pig isolated ileum.  相似文献   

7.
Rhythmic application of acetylcholine or serotonin to the local zone of somatic membrane was used to study the effect of extinction of RPa4 neuron depolarization in Helix lucorum on the excitability of adjacent chemo- and electroexcitable zone. It has been found that the extinction of response to iontophoretic application of acetylcholine to one somatic zone decreases the sensitivity of serotonin and cholinoreceptors in adjacent zones, as well as the excitability of electroexcitable membrane. The effect on the excitability of adjacent zones does not depend on the type of receptors activated rhythmically, as the extinction of RPa4 response to the repeated application of serotonin also reduces the sensitivity of adjacent cholinoreceptor zones. A cause of this effect may lie in modification of chemoreceptors and ionic channels, by intracellular regulatory systems that become activated by repeated stimulation.  相似文献   

8.
Changes in membrane potential and conductance were studied in neurons of isolated sympathetic ganglia ofRana ridibunda during perfusion with cholinomimetics and cholinolytics. Activation of nicotinic (N) acetylcholine receptors by carbachol, suberyldicholine, and tetramethylammonium led to depolarization with an increase in conductance, whereas activation of muscarinic (M) acetylcholine receptors by perfusion with carbachol or 5-methylfurmethide, led to depolarization with a decrease or (less frequently) an increase in conductance. The M-cholinolytic atropine was shown to cause depolarization with an increase in conductance if perfusion with atropine was preceded by perfusion with carbachol.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 11, No. 5, pp. 475–482, September–October, 1979.  相似文献   

9.
Effects of acetylcholine (ACh), ACh-agonists and antagonists were studied on the viscosity of the dermis of the sea cucumber Holothuria leucospilota. ACh and nicotinic agonists caused an early increase in viscosity and late decrease. Muscarinic agonists produced a viscosity decrease. The viscosity increase elicited by nicotine was inhibited by tubocurarine. The viscosity decrease caused by methacholine was suppressed by atropine. The mechanical properties of this connective tissue are very likely controlled by both nicotinic and muscarinic cholinoreceptors.  相似文献   

10.
The effects of nicotinic and muscarinic mimetics and lytics on spontaneous quantal transmitter secretion from the motor nerve endings were investigated during experiments on theRana temporaria sartorius muscle. Acetylcholine and carbachol reduced the frequency of miniature endplate potentials both in a normal ionic medium and in one with potassium ion concentration raised to 10 mM. Similar effects were produced by nicotinic agonists, namely nicotine, tetramethylammonium, and suberyldicholine, whereas muscarinic mimetics — methylfurmetide, oxotremorine, and F-2268 (L- and D-stereoisomers) — did not affect transmitter release. Neither d-tubocurarine, benzohexonium, nor atropine abolished the presynaptic effects of carbachol and acetylcholine. It is concluded that nicotinic cholinoreceptors are present at the frog motor nerve endings which modify spontaneous transmitter release and differ in their pharmacological properties from recognized N-cholinoreceptors of the motor and autonomic systems of the higher vertebrates.S. V. Kurashov Medical Institute, Ministry of Public Health of the RSFSR, Kazan'. Translated from Neirofiziologiya, Vol. 18, No. 5, pp. 586–593, September–October, 1986.  相似文献   

11.
In experiments on isolated and identified neurones of the pedal ganglion of the gastropod mollusc P. corneus, it was demonstrated that biphasic response to ACh may be obtained both to superfusion and electrophoretic application. Fast (depolarizing) phase may be imitated by a nicotinomimetic drug, suberyldicholine, and blocked by d-tubocurarine. These data indicate that this phase results from activation of nicotinic cholinoreceptors. Slow (hyperpolarizing) phase may be evoked by a muscarinomimetic, dioxolane F-2268, which is taken as an indication of the muscarinic nature of cholinoreceptors responsible for this phase. Experiments on completely isolated neurones directly show that both kinds of cholinoreceptors belong to the same neurone. Biphasic pattern of the response depends on the level of the membrane potential and on the conditions of ACh application. The fast phase is more evident at membrane hyperpolarization, the slow one--at depolarization. The fast phase is more readily obtained by superfusion by high concentrations of ACh or at close position of electrophoretic micropipette to neuronal surface, whereas the slow phase may be easily obtained by superfusion with low concentrations of ACh or when the micropipette is not attached so closely to the cell.  相似文献   

12.
Pharmacological properties of excitatory synaptic transmission from mechanosensory afferents to an identifiable nonspiking interneuron of crayfish were studied by drug perfusion experiments using acetylcholine (ACh) agonists and antagonists. Application of carbachol, a general agonist of ACh, caused sustained depolarization of the interneuron and a decrease in the peak amplitude of its excitatory synaptic response to sensory stimulation on the soma side. Similar depolarization was observed during application of carbachol under the low-Ca2+, high-Mg2+ condition. The peak amplitude was also reduced by application of nicotine and tetramethylammonium, both of which also caused sustained depolarization of the inter-neuron. By contrast, perfusion of muscarinic agonists, muscarine, oxotremorine and pilocarpine, reduced the peak amplitude without affecting the membrane potential of the interneuron. Perfusion of nicotonic antagonists of ACh, d-tubocurarine and hexamethonium, caused reduction of the peak amplitude without any change in the membrane potential. A muscarinic antagonist atropine was also effective in blocking the synaptic transmission but at higher concentration than d-tubocurarine. The results suggest that the ACh receptors on the nonspiking interneuron belong to a previously characterized class of crustacean cholinergic receptors resembling the nicotinic subtype of vertebrates.  相似文献   

13.
We studied the effects of nicotine and cytisine injected i.p. in equimolar doses (0.5 mol/kg) on the formation and extinction of the conditioned active avoidance reflex (AAR) in rats; a combination of conditioning acoustic stimulation and unconditioned nociceptive electrocutaneous stimulation was used for training. A stable AAR was formed more rapidly in the case of nicotine injections; the corresponding effect of cytisine injections did not attain a significance level (probably because of the weak effect of this cholinomimetic on AAR formation in slowly trained animals present in the tested group). Injections of both nicotine and cytisine in the course of formation of a stable AAR led to a significantly slower extinction of the conditioned reflex habit. Both cholinomimetics demonstrated such an effect with respect to both the entire animal population and rats with rapid extinction of the habit. Cytisine-induced slowing down of extinction of the AAR was more significant than the corresponding effect of nicotine. We postulate that the positive effects of cytisine and nicotine on maintenance of the AAR are mediated by activation of neuronal nicotine cholinoreceptors having the 34 subunit structure.  相似文献   

14.
Selyanko  A. A.  Derkach  V. A.  Dé Kurennyi  D. A.  Skok  V. I. 《Neurophysiology》1988,20(5):493-499
The effects of tubocurarine (TC) on current induced by acetylcholine (ACh) in neurons of rat upper cervical ganglia were investigated using techniques for voltage-clamping at the membrane. Reinforcement of TC-induced blockade was achieved by paired application of ACh following prior activation of nicotinic cholinoreceptors, indicating that TC blocked the channels opened by ACh. On average, the TC-open channel complex persisted for 9.8±0.5 sec (n=7) at –50 mV and 20–24°C. It was found that increases exponentially with hyperpolarization at the membrane (a shift in membrane potential of 61 mV corresponds to an e-fold change). Suppression of ACh-induced current (ACh current) was eliminated completely under the effects of 3–30 M with depolarization of up to 80–100 mV at the membrane. Suppression of ACh current produced by membrane potential at negative levels is intensified with increasing doses of ACh. Findings would indicate that blockade of ionic channels opened by ACh is the only mechanism of TC action on nicotinic cholinoreceptors in rat sympathetic ganglia.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 20, No. 5, pp. 672–680, September–October, 1988.  相似文献   

15.
1. The effects of cholinergic drugs on catecholamine (CA) secretion from adrenal chromaffin tissue of the toad were studied. 2. CA secretion was induced by ACh or nicotine, but not by muscarine. 3. Hexamethonium inhibited the CA release evoked by ACh or nicotine, while d-tubocurarine only affected the nicotinic response. Atropine did not prevent the secretory response. 4. Muscarine abolished the secretion induced by the agonists, this effect being prevented by atropine or gallamine, but not by pirenzepine. 5. In conclusion, CA secretion in the toad is stimulated by activation of nicotinic receptors. Inhibitory muscarinic receptors are present, most likely of type M2, which may play a regulatory function.  相似文献   

16.
Possible role of cGMP is studied in control of extinction of snail neurones RPa4, RPa3 and LPa3 reactions to acetylcholine (ACh), applied rhythmically to neurone soma by means of microiontophoresis. It is shown that guanylate cyclase activators which raise the cGMP level in the cell--Na nitroprusside and Na azide (5,10(-4)-10(-3) mol/l)--intensify at extracellular application the extinction of inward transmembrane current and membrane depolarization in response to ACh. Suggestion is made about participation of cGMP-dependent phosphorylation of membrane proteins in control of the development rate, depth and duration of neurone cholinoreceptors short-term plasticity.  相似文献   

17.
1. The effects of cholinergic drugs on catecholamine (CA) secretion from adrenal chromaffin tissue of the toad were studied.2. CA secretion was induced by ACh or nicotine, but not by muscarine.3. Hexamethonium inhibited the CA release evoked by ACh or nicotine, while d-tubocurarine only affected the nicotinic response. Atropine did not prevent the secretory response.4. Muscarine abolished the secretion induced by the agonists, this effect being prevented by atropine or gallamine, but not by pirenzepine.5. In conclusion, CA secretion in the toad is stimulated by activation of nicotinic receptors. Inhibitory muscarinic receptors are present, most likely of type M2, which may play a regulatory function.  相似文献   

18.
1. Acetylcholine receptors were initially defined as nicotinic or muscarinic, based on selective activation by two natural products, nicotine and muscarine. Several further nicotinic agonists have been discovered from natural sources, including cytisine, anatoxin, ferruginine, anabaseine, epibatidine, and epiquinamide. These have provided lead structures for the design of a wide range of synthetic agents.2. Natural sources have also provided competitive nicotinic antagonists, such as the Erythrina alkaloids, the tubocurarines, and methyllycaconitine. Noncompetitive antagonists, such as the histrionicotoxins, various izidines, decahydroquinolines, spiropyrrolizidine oximes, pseudophrynamines, ibogaine, strychnine, cocaine, and sparteine have come from natural sources. Finally, galanthamine, codeine, and ivermectin represent positive modulators of nicotinic function, derived from natural sources.3. Clearly, research on acetylcholine receptors and functions has been dependent on key natural products and the synthetic agents that they inspired.  相似文献   

19.
Superfusion of the isolated sympathetic ganglia of the frog with nicotinic agonists (suberyldicholine, tetramethylamonium, and dimethylphenylpiperazinium), as well as acetylcholine in the presence of atropine led to a brief depolarization of the neurons and blockade of synaptic transmission. The muscarinic agonists methylfurmethide (MFM) and methyldilvasen, cis, L(+), as well as acetylcholine elicited a stable depolarization which is not accompanied by disturbance in transmission. Oxotremorine at a concentration of 1·10–5 M did not lead to the depolarization of the post-synaptic membrane, but at a concentration of 1·10–6 M decreased the quantal EPSP content twofold, which indicates that the presynaptic receptors belong to the M2 subtype. Inhibition of acetylcholinesterase significantly intensified the postsynaptic effect of MFM: a shift of the concentration-effect curve took place toward the side of lower MFM concentrations. It was shown that the post-synaptic muscarinic receptors of the ganglionic neurons possess varied sensitivity to the enantiomers of methyldilvasen and, consequently, are stereospecific. The identified functional properties of the cholinoreceptors of the ganglionic neurons explain the set of changes in synaptic transmission under conditions of the prolonged presence of a mediator in the synaptic cleft.I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of the USSR, Leningrad. Translated from Neirofiziologiya, Vol. 20, No. 2, pp. 227–234, March–April, 1988.  相似文献   

20.
We studied modulatory effects of the cholinergic system on the activity of sensorimotor cortex neurons related to realization of an instrumental conditioned placing reflex. Experiments were carried out on awake cats; multibarrel glass microelectrodes were used for extracellular recording of impulse activity of neurons in the sensorimotor cortex and iontophoretic application of synaptically active agents within the recording region. The background and reflex-related activity was recorded in the course of realization of conditioned movements, and then changes of spiking induced by applications of the testing substances were examined. Applications of acetylcholine and carbachol resulted in increases in the intensity of impulse reactions of neocortical neurons evoked by presentation of an acoustic signal and in simultaneous shortening of the response latencies. An agonist of muscarinic receptors, pylocarpine, exerted a similar effect on the evoked activity of sensorimotor cortex neurons. Blockers of muscarinic receptors, atropine and scopolamine, vice versa, sharply suppressed impulse reactions of cortical neurons to afferent stimulation and simultaneously increased latencies of these responses. Applications of an agonist of nicotinic receptors, nicotine, was accompanied by suppression of impulse neuronal responses, an increase in the latency of spike reactions to presentation of a sound signal, and a corresponding increase in the latency of a conditioned motor reaction. In contrast, application of an antagonist of nicotinic receptors, tubocurarine, significantly intensified neuronal spike responses and shortened their latency. The mechanisms underlying the effects of antagonists of membrane muscarinic and nicotinic cholinoreceptors and the role of activation of these receptors in the modulation of activity of pyramidal and non-pyramidal neocortical neurons related to realization of the instrumental motor reflex are discussed.  相似文献   

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