Elm bark beetle boring and feeding deterrents from Phomopsis oblonga

The two groups of Phomopsis oblonga which invade the phloem of stressed elm trees have been investigated for the production in vitro of boring/feeding deterrents for elm bark beetles. The secondary metabolites of this fungus are compared with those of closely-related Phomopsis spp. associated with ash and sycamore. Active compounds isolated from P. oblonga include a novel norsesquiterpene γ-lactone, the tiglic esters of two novel 5,6-dihydro-5-hydroxy-2-pyrones, nectriapyrone, 4-hydroxyphenylethanol, 5-methylmellein, 2-furoic, orsellinic and 3-nitropropanoic acids and mellein-5-carboxylic acid; portensterol and thymine, from P. oblonga, were inactive. (+)-Mellein and furan-2,5-dicarboxylic acid were obtained as minor metabolites of the ash and sycamore Phomopsis strains, which also produced the norsesquiterpene γ-lactone and one of the tiglic esters.     

The effect of bark phenols upon mountain hare barking of winter-dormant Scots pine

Due to high numbers of mountain hares in recent winters in northern Finland the barking of winter dormant Scots pines was widespread. The hares fed selectively upon upper crown bark of grafted pines, nitrogen fertilized trees and trees in poor condition. Bark of preferred upper crown branches and physiologically mature scions contained less total phenols than lower parts. However, there were no differences between total phenols concentrations of graft bark preferred by hares and nearby unbarked juvenile phase Scots pine. Neither decrease in resistance after nitrogen fertilization n- or stress was correlated with bark total phenolic concentration. Thus, total phenolic concentration is not a reliable predictor of the susceptibility of Scots pine to winter barking by the mountain hare.     

Adult beetle grazing induces willow trichome defence against subsequent larval feeding

Induced increases in trichome density to grazing by herbivores have been suggested to increase plant resistance to future herbivore attack. In this paper we present results which show that plants of Salix cinerea L. respond to adult leaf beetle (Phratora vulgatissima L.) grazing by developing new leaves with an increased trichome density. The same plants are usually attacked again later in the season when the next generation of larvae hatches on the plants. The effect of the induced response was studied by comparing larval growth and feeding on newly developed leaves of plants exposed to different defoliation treatments. Larvae on plants previously exposed to adult grazing consumed less total leaf area and showed more dispersed feeding than larvae on plants protected from previous grazing. Larvae on plants exposed to mechanical defoliation responded intermediately. These results corresponded to the increased trichome density of defoliated plants. However, we found this larval response only in whole plant tests--when reared on single, excised leaves in petri dishes, larvae in all treatments behaved similarly. This discrepancy between the on-plant experiment and that in petri dishes highlights how experimental design may alter the conclusion of a study. We suggest that the induced response to adult grazing may act as a defence against subsequent larval feeding.     

Shelter characteristics of mountain hare resting sites

Mountain hares in Scotland live on open tree-less moorland in a harsh winter environment with frequent high winds and drifting snow. By day they rest in forms in tall heather, seats in shorter heather or snow, or scrapes in snow or peat. All these reduce wind speeds by 84-90%, but vary in the degree of shelter and concealment. Forms afford the best concealment and shelter and occur as often on flat ground as elsewhere. Seats and scrapes are commoner on sheltered slopes. They command a wide field of view but offer little concealment. During snow-lie, when forms may be covered, hares move to seats and scrapes, often at the edge of snowfields and gather, sometimes in large groups, on sheltered slopes.     

Phenolic glucosides as feeding cues for willow-feeding leaf beetles

The effects of individual phenolic glucosides and total glucoside fractions on the feeding behaviour of three willowfeeding leaf beetles (Coleoptera: Chrysomelidae) were tested in the laboratory. Feeding preferences of the tested leaf beetles were strongly influenced by certain phenolic glucosides which are typical secondary compounds of willows (Salicaceae:Salix). Two of the tested leaf beetles,Galerucella lineola andLochmaea capreae showed rather similar responses to glucoside treatments. Both of them were strongly stimulated by total glucoside fractions fromSalix triandra and by its major glucoside salidroside. The third species,Phatora vitellinae, was attracted most by the fractions fromS. myrsinifolia andS. pentandra, and by two related salicylate glucosides, tremulacin and salicortin. Food selection pattern of the tested beetles in the laboratory concords fairly well with their distributions in the field and with the occurrence of phenolic glucosides in their host willows. Phenolic glucoside extracts stimulated more feeding than individual pure glucosides. This indicates that different compounds have synergistic effects in the feeding behaviour of leaf beetles. Our results clearly show that willow leaf beetles select their food based on phenolic glucosides of their host plants.     

Inter- and intraspecific variation in the resistance of winter-dormant birch (Betula spp.) against browsing by the mountain hare

The palatability of saplings of several different species, geographic origins and F₂-families of Betula spp. to the mountain hare was tested in feeding trials with captive and free-ranging animals. Significant variation in the palatability was detected among species and among conspecifics representing different origins and families. The results show that the combination of genetic and environmental factors determines the resistance of individual plants to mammals. Saplings that were stressed by severe competition from surrounding weeds were more resistant than saplings of the same age grown under optimal conditions. One-yr-old seedlings were more resistant than 7-yr-old saplings of the same origin. Some of the exotic species tested were extremely resistant, whereas others were highly palatable. The most resistant species and origins came from Asia and Alaska.     

Two 2-arylbenzofurans as insect feeding deterrents from sainfoin (Onobrychis viciifolia)

Two 2-arylbenzofurans have been isolated as insect feeding deterrents from the roots of the forage legume, sainfoin. They have been identified as 2-(2'-hydroxy-4'-methoxyphenyl)-5-hydroxy-6-methoxybenzofuran (sainfuran) and 2-(2',4'-dimethoxyphenyl)-5-hydroxy-6-methoxybenzofuran (methylsainfuran). Their synthesis is described.     

Phenolic compounds from Bursera simaruba Sarg. bark: Phytochemical investigation and quantitative analysis by tandem mass spectrometry

Phytochemical investigation of the methanolic extract of Bursera simaruba bark led to the isolation of 11 compounds, including lignans yatein, β-peltatin-O-β-d-glucopyranoside, hinokinin and bursehernin, and three natural compounds namely 3,4-dimetoxyphenyl-1-O-β-d-(6-sulpho)-glucopyranoside, 3,4,5-trimetoxyphenyl 1-O-β-d-(6-sulpho)-glucopyranoside and 3,4-diidroxyphenylethanol-1-O-β-d-(6-sulpho)-glucopyranoside. Their structures were established by NMR and ESI/MS experiments. Additionally, an LC-ESI/MS qualitative study on the phenolic compounds and an LC-ESI/MS/MS quantitative study on the lignans found in the methanolic extract of B. simaruba bark were performed to give value to the plant as source of these biological active compounds. Quantitative analyses results confirmed that compounds yatein, β-peltatin-O-β-d-glucopyranoside, hinokinin and bursehernin are major compounds in the bark and, in particular, β-peltatin-O-β-d-glucopyranoside appears to be the most abundant.     

Screening of crude plant extracts as feeding deterrents of the wireworm, Melanotus communis

Extracts from 78 plant species (24 families) were screened for antifeedant activity against the corn wireworm, Melanotus communis Gyll. (Coleoptera: Elateridae). Five extracts from four families significantly reduced wireworm feeding damage in a series of choice feeding tests. Two extracts, Asclepias tuberosa and Hedera helix, exhibited exceptional levels of feeding deterrency. An x-ray technique indicated that wireworms burrowed indiscriminately between soil containing either of these extracts and surrounding untreated soil, but were found more frequently in the untreated areas.

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Résumé L'aptitude à empêcher la prise de nourriture de Melanotus communis Gyll. a été recherchée dans les extraits de 78 plantes appartenant à 24 familles. Cinq extraits de 4 familles ont réduit significativement les dégâts alimentaires lorsqu'il y avait choix alimentaire. Deux extraits — Asclepias tuberosa et Hedera helix — présentaient des niveaux exception-nellement élevés de dissuasion de l'alimentation. Une technique aux rayons X a montré que les taupins fouillaient indistinctement le sol contenant de ces extraits ou le sol avoisinant non traité, mais on les recontrait plus fréquemment dans le sol non traité.

     

Investigating the effectiveness of feeding deterrents against the southern corn rootworm, using behavioral bioassays and toxicity testing

Leaf-disk feeding tests were conducted to determine the effect of various chemicals on southern corn rootworm, Diabrotica undecimpunctata howardi Barber, larval feeding. Ethanolic extracts of plant seeds from the family Meliaceae were all highly active feeding deterrents, while hexane extracts were ineffective as deterrents. In leaf disk feeding tests, the fungicide thiram (tetramethylthiuram disulfide), as technical material or formulated as thiram 50% or 75% (AI) was also an effective feeding deterrent. In choice tests, all thiram-based deterrents were equally effective, while in no-choice tests, thiram-75 was most effective at preventing feeding. Thiram-75 was also effective in protecting corn seedlings in a soil bioassay mimicking the field environment. It was determined that the formulation of thiram-75 used in these tests had been contaminated with 0.87% dieldrin (by weight). The contaminated thiram-75 had a toxic action, as well as a deterrent effect on southern corn rootworm larvae and both factors contributed to overall feeding inhibition. Toxicity was not found in technical thiram or in thiram-50. The mode(s) of action of the various deterrents are discussed in relation to screening procedures.

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Resumé Des expériences de consommation avec et sans choix ont été utilisées pour déterminer l'effet de différentes substances sur la prise de nourriture par D. undecimpunctata howardi Barber. Des disques de feuilles de Zea mays avec dissuadants ont été présentés à des chenilles du troisième stade; l'état des disques a été examiné au bout de 3,6 et 24 h dans les expériences de choix et de 48 et 72 h en absence de choix.Les extraits éthanoliques (1% dans l'éthanol) de Méliacées sont tous très efficaces, tandis que ceux à l'hexane (1% dans le pentane) sont inefficaces. Le thiram (disulfure de tétraméthylethiuram) utilisé comme matériel technique, à 50% ou à 75%, est lui aussi efficace (à 0,5% ou 1% dans l'acétone). Lorsqu'il y a choix tous les thirams sont aussi dissuasifs, alors qu'en absence de choix le thiram 75% paraît le plus efficace. Lorsque ce dernier est contaminé par 0,87% en poids de dieldrine, il est efficace lors d'expériences simulant le sol du champ. La contamination par la dieldrine rend le thiram 75 toxique autant que dissuadant. Cette toxicité n'est pas observée sur le thiram technique ou le thiram 50. Les modes d'action des différents dissuadants sont discutés.

     

Phenolic compounds isolated from Dioscorea zingiberensis protect against pancreatic acinar cells necrosis induced by sodium taurocholate

One new bibenzyl (1) and one new phenanthrene (2), together with two known bibenzyls (3–4) and four known diarylheptanoids (5–8) were isolated from the rhizomes of Dioscorea zingiberensis. The structures of 1–2 were elucidated by spectroscopic methods including 1D and 2D NMR. Phenols 1–8 were evaluated for their anti-pancreatitic activities on sodium taurocholate (NaT)-induced pancreatic acinars necrosis. Notably, 0.5 mM of compound 6 exhibited comparable inhibitory effect with 5 mM of caffeine. Furthermore, compound 6 prevented the ATP depletion and excessive ROS production which could be also involved in mitochondria-mediated injuries in acute pancreatitis. As a result, compound 6 has been demonstrated to be a potential candidate for mediating mitochondrial dysfunction to prevent pancreatic necrosis. This study is also the first report on the isolation of bibenzyls and diarylheptanoids from this plant.     

The occurrence of mountain hare mitochondrial DNA in wild brown hares

If interspecific hybrids are fertile and backcross to either parental species, transmission of mitochondrial DNA over the species barrier can occur. To investigate if such transmission has occurred between the brown hare Lepus europeus Pall and the mountain hare L. timidus L. in Scandinavia, an analysis of genetic variation in mitochondrial DNA from 36 hares, collected from 15 localities, was performed. Sequence divergence of mtDNA between species was estimated at 8 ± 1% (SD). Intraspecific mtDNA sequence divergence varied between 0.09 and 0.38% in brown hares and 0.10 and 1.44% in mountain hares. In six out of 18 brown hares examined, two different haplotypes of mountain hare origin were detected, demonstrating a transmission of mtDNA haplotypes from mountain hares to brown hares. The results indicate that interspecific hybridization between the two species occurs in wild populations.     

Laboratory and field evaluation of insect repellents as oviposition deterrents against the mosquito Aedes albopictus

Three experimental approaches were used to evaluate the oviposition deterrency of three insect repellents, AI3-35765, AI3-37220 (piperidine compounds), and the standard N,N-diethyl-3-methylbenzamide (deet) to the mosquito Aedes albopictus Skuse (Diptera: Culicidae). Against laboratory-reared Ae. albopictus gravid females, the EC50 values of AI3-37220, AI3-35765 and deet were 0.004%, 0.008% and 0.011% in laboratory cages and 0.004%, 0.01% and 0.009% in an outdoor screened cage. For a natural population of Ae. albopictus tested in the field, the EC50 values were determined as 0.004%, 0.008% and 0.001%, respectively. Ageing concentrations of 0.1% of each repellent provided >50% effective oviposition deterrency against the laboratory population of Ae. albopictus for 13 days in laboratory cages, for 15 days in the outdoor cage, and for 21 days against field population of Ae. albopictus in Florida. These topical skin repellents are effective oviposition deterrents for Ae. albopictus when employed at relatively low application rates.     

HPLC-MS/MS analysis of willow bark extracts contained in pharmaceutical preparations

Preparations containing willow bark extract are popular herbal remedies, but they are mostly standardised with respect to only one compound (usually salicin). RP-HPLC using a C18-column eluted with water:methanol:tetrahydrofuran and coupled to electrospray triple-quadrupole MS and MS/MS was used for the characterisation of dried extracts of Salix spp. and for the identification of their constituents. Comparison with reference substances led to the identification of 13 compounds (saligenin, salicylic acid, salicin, isosalicin, picein, salidroside, triandrin, salicoylsalicin, salicortin, isosalipurposide, salipurposide, naringenin-7-O-glucoside and tremulacin). Two pharmaceutical preparations containing willow bark extract, used in clinical trials and labelled Salix daphnoides and S. purpurea x daphnoides extracts, were compared using the described method and exhibited several clear differences, the most prominent of which was the possible presence of picein in the former preparation. The described method may be utilised for the characterisation of herbal medicines in order to ensure comparability of medication in further clinical trials.     

The role of an epipharyngeal sensillum in the perception of feeding deterrents by Leptinotarsa decemlineata larvae

An epipharyngeal taste sensillum in Leptinotarsa decemlineata larvae was studied. Electron microscopy showed that the sensillum is innervated by five neurons. Electrophysiological experiments showed that one of these cells responds to water, a second to sucrose and a third to two feeding deterrents that were also effective in a behavioural test. Receptor cells sensitive to feeding deterrents were not previously reported for L.␣decemlineata larvae or adults. The response of the sucrose-sensitive cell was strongly inhibited by one of the two feeding deterrents and only slightly by the other feeding deterrent. The relationship between the behavioural and electrophysiological results is discussed in order to elucidate the neural code of feeding deterrents in L. decemlineata larvae. We conclude that probably both the response of the deterrent cell and peripheral interactions exerted by feeding deterrents on the sucrose-sensitive cell determine the potency of feeding deterrents. The present results provide a physiological basis for the hypothesis that the presence or absence of feeding deterrents in potential food plants is a decisive cue in food plant selection by L. decemlineata larvae. Accepted: 25 March 1998     

Numerical responses by populations of red fox and mountain hare during an outbreak of sarcoptic mange

Summary During a severe outbreak of sarcoptic mange (Sarcoptes scabiei vulpes) starting among red foxes (Vulpes vulpes) in Sweden in the 1970s, we studied: 1) the establishment and spread of the disease in northernmost Sweden (by inquiries), and 2) the 1970–84 bag records for foxes and mountain hares (Lepus timidus) (an alternative prey to the fox's main prey, voles). Since the first case of sarcoptic mange in 1975 the disease spread rapidly, with >50% of the hunting organizations having reported the disease in 1981 and >75% in 1983. Also the disease became more abundant within the areas affected. In areas with a low mange infection rate (index) the number of foxes killed in the 1980s did not deviate markedly from the average level in the 1970s. However, there was a slight tendency towards a decline in areas with a medium index and numbers declined markedly where the index was high. Hare harvests initially were low (after a tularemia epidemic) in the 1970s. In that decade harvests increased dramatically and stabilized, increased gradually or changed little, respectively, where mange infection rates were low, medium or high in the early 1980s. In areas with a low mange index hare harvests remained cyclical and at the same level in the 1980s as in most of the 1970s. However, in areas with a medium index harvests increased and seemed to begin to lose their cyclicity, and where the index was high the low and relatively stable hare harvests increased annually. A predator-prey hypothesis, assuming predators to synchronize alternative prey declines to those of the cyclic main prey, predicts that a predator reduction would cause a gradual disappearance of the cyclicity and increasing numbers among alternative prey. Our hare data are partially consistent with this prediction.