乌司他丁联合生长抑素辅助连续血液净化对重症胰腺炎患者血清HSP70、IL-15、HMGB1水平的影响

目的:研究乌司他丁联合生长抑素辅助连续血液净化对重症胰腺炎患者血清热休克蛋白70(Heat shock protein 70,HSP70)、白介素-15(Interleukin-15,IL-15)、高迁移率族蛋白B1(high mobility group protein1,HMGB1)水平的影响。方法:选择2018年5月～2019年10月在我院就诊的82例重症胰腺炎患者,依据入院先后顺序分为对照组(40例)和研究组(42例)。对照组采用生长抑素辅助连续血液净化治疗,研究组采用乌司他丁联合生长抑素辅助连续血液净化治疗。观察和比较两组临床疗效,症状改善时间,治疗前后血清白蛋白(albumin,Alb)、尿素氮(Urea nitrogen,BUN)、血肌酐(Serum creatinine,Scr)、血淀粉酶(amylase,AMY)、HSP70、IL-15、HMGB1水平及急性生理学及慢性健康状况(APACHEⅡ)评分的变化情况和不良反应的发生情况。结果:治疗后,研究组总有效率显著高于对照组(P0.05),症状改善时间及住院时间均少于对照组(P0.05)。治疗后,两组Alb均较治疗前显著上升,BUN、Scr、AMY、APACHEⅡ评分、血清HSP70、IL-15、HMGB1水平均较治疗前明显下降,研究组以上指标的变化较对照组更明显(P0.05)。两组不良反应总发生率比较无统计学差异(P0.05)。结论:乌司他丁联合生长抑素辅助血液净化有利于重症胰腺炎患者病情恢复,减轻临床症状,降低HSP70、IL-15和HMGB1水平。     

乌司他丁联合前列地尔对急性胰腺炎的临床疗效及对血清TNF-α、IL-1β、HMGB1、HSP的影响

目的:探讨乌司他丁联合前列地尔治疗急性胰腺炎的临床疗效及对血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素(Interleukin-1 beta,IL-1β)、高迁移率组蛋白1(HMGB1)、热休克蛋白(HSP)的影响。方法:选择72例我院收治的急性胰腺炎患者并将其随机平均分为观察组和对照组。对照组按照常规方法治疗,观察组在常规治疗方法上采用乌司他丁联合前列地尔加入葡萄糖注射治疗。比较两组患者临床症状及体征恢复时间、治疗前后血清TNF-α、IL-1β、HMGB1、HSP水平的变化及血淀粉酶水平的恢复情况。结果:治疗后,观察组的总有效率为97.2%,对照组的总有效率为69.7%,观察组显著高于对照组(P0.05);观察组多个体征恢复时间均明显短于对照组(P0.05);两组血清TNF-α、IL-1β、HMGB1、HSP水平均较治疗前明显下降,且观察组以上指标均显著低于对照组(P0.05);观察组血淀粉酶水平恢复率明显高于对照组(P0.05)。结论:乌司他丁联合前列地尔治疗急性胰腺炎能有效提高其临床疗效,显著缩短临床症状及体征恢复时间、血淀粉酶恢复时间,降低患者血清TNF-α、IL-1β、HMGB1、HSP水平。     

多囊卵巢综合征患者血清HSP70、IMA水平的变化及与性激素和氧化应激的相关性研究

目的:探讨多囊卵巢综合征(PCOS)患者血清热休克蛋白70(HSP70)、缺血修饰白蛋白(IMA)水平的变化及与性激素指标和氧化应激指标的相关性。方法:选择2017年6月~2018年6月华中科技大学同济医学院附属同济医院收治的未经治疗的初诊PCOS患者87例为PCOS组,另随机选择同期在本院进行体检的健康育龄妇女87例为对照组,比较两组血清HSP70、IMA、性激素指标、氧化应激指标,分析血清HSP70、IMA水平与性激素、氧化应激的相关性。结果:PCOS组患者血清卵泡刺激素(FSH)、促黄体生成素(LH)、睾酮(T)、8-异前列腺素F2α(8-isoPGF2α)、丙二醛(MDA)、HSP70、IMA水平显著高于对照组,差异有统计学意义(P<0.05);PCOS组患者血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSH-Px)水平低于对照组,差异有统计学意义(P<0.05);PCOS组血清雌二醇(E2)、泌乳素(PRL)水平与对照组比较差异无统计学意义(P>0.05)。PCOS患者血清HSP70、IMA水平与血清FSH、LH、T、8-iso PGF2α、MDA水平呈正相关关系,与SOD、GSH-Px水平呈负相关关系(P<0.05),与E2、PRL水平无显著相关性(P>0.05)。结论:血清HSP70、IMA水平在PCOS患者呈现异常升高,其水平改变与性激素及氧化应激水平密切相关,提示HSP70、IMA可能参与了PCOS的病理过程,临床检测HSP70、IMA的水平变化有可能为PCOS诊疗提供有效的预测作用。     

激光佐治慢性皮肤溃疡疗效观察及诱导HSP70表达研究

目的:探讨激光治疗慢性皮肤溃疡的疗效及诱导创面组织热休克蛋白70(heat shock prote in 70,HSP70)表达情况。方法:将64例84处慢性皮肤溃疡创面随机分为传统治疗组和激光治疗组,激光治疗组在传统治疗基础上加用激光治疗,所有创面在治疗三周进行疗效判定;同期随机切取5例创面组织,并设正常皮肤为对照,行组织切片HSP70免疫组化染色,计数HSP70阳性细胞数并检测阳性细胞灰度值进行统计学分析;设计引物行RT-PCR检测HSP70mRNA表达情况。结果:两组慢性皮肤溃疡患者经相应治疗均较治疗前明显改善,但激光治疗组创面的治愈率和总有效率明显高于传统治疗组(P<0.05或P<0.01);免疫组化染色显示,正常皮肤和慢性溃疡组织中未见明显HSP70阳性表达细胞,激光治疗后三周后可见较多量的HSP70表达阳性细胞,其阳性细胞计数明显高于其它两组(P<0.01),细胞信号灰度表达明显低于其它两组(P<0.05),而传统治疗组与正常对照组无显著差异;RT-PCR结果发现激光治疗组组织中扩增到一条特异性泳带,大小约268 bp,正常皮肤和传统治疗组组织中未扩增到明显HSP70mRNA基因片段。结论:激光佐治慢性皮肤溃疡具有良好临床疗效,可能与激活创面细胞的热休克内源性保护机制而发挥抗感染和促进愈合作用有关。     

热休克蛋白70在原发性高血压中的表达及其与炎症因子和血压的关系

目的:探讨原发性高血压患者热休克蛋白70(HSP-70)的表达水平,并分析其与炎症因子、血压的关系。方法:选择2017年3月至2019年3月我院收治的原发性高血压患者84例作为研究组,根据患者血压水平分为Ⅰ级组25例,Ⅱ级组37例和Ⅲ级组22例,另选同期体检健康者60例作为对照组,应用逆转录聚合酶链式反应(RT-PCR)检测各组血HSP70 m RNA水平,应用双抗体酶联免疫吸附法检测各组血清HSP-70、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和C反应蛋白(CRP)水平,比较各组血HSP70 m RNA、血清HSP70、IL-6、TNF-α和CRP水平,并分析相关性。结果:研究组血HSP-70mRNA相对表达量高于对照组(P0.05)。随高血压分级的升高各组患者血HSP-70mRNA相对表达量呈升高趋势,各组血HSP-70mRNA水平比较有统计学差异(P0.05)。研究组血清HSP-70、IL-6、TNF-α和CRP水平均高于对照组(P0.05)。随高血压分级的升高,各组患者血清HSP-70、IL-6、TNF-α和CRP水平呈升高趋势,不同组别血清HSP-70、IL-6、TNF-α和CRP水平比较有统计学差异(P0.05)。经Pearson相关分析显示,高血压患者血HSP-70 m RNA、HSP-70、IL-6、TNF-α和CRP水平与血压呈正相关(P0.05),血HSP-70 m RNA与IL-6、TNF-α和CRP水平呈正相关(P0.05),血清HSP-70与IL-6、TNF-α和CRP呈正相关(P0.05)。结论:高血压患者HSP-70和炎症因子异常升高,HSP-70与炎症因子水平和血压相关,提示HSP-70在高血压发生、发展中起到关键作用。     

醋酸奥曲肽联合乌司他丁对重症急性胰腺炎患者血清ET、MCP-1、TNF-α、IL-6水平及预后的影响

目的:探讨醋酸奥曲肽联合乌司他丁对重症急性胰腺炎血清内皮素(ET)、单核细胞趋化因子蛋白1(MCP-1)、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平及预后的影响。方法:纳入我院2017年1月至2018年9月收治的94例重症急性胰腺炎患者,并依据随机数字表法将其分为对照组47例与观察组47例。对照组患者给予乌司他丁治疗,观察组在对照组基础上结合醋酸奥曲肽治疗,两组疗程均为7～14 d。比较两组治疗的疗效,血淀粉酶和尿淀粉酶恢复正常时间,腹痛缓解时间、肠鸣音恢复时间和腹胀缓解时间,治疗前后血清ET、MCP-1、TNF-α、IL-6水平的变化及预后。结果:观察组治疗总有效率(93.62%)显著高于对照组(72.34%)(P0.05)。观察组血淀粉酶和尿淀粉酶恢复时间、腹痛缓解时间、肠鸣音恢复时间和腹胀缓解时间明显短于对照组(P0.05)。两组治疗后血清ET、MCP-1、TNF-α和IL-6水平均较治疗前降低(P0.05),且观察组以上指标均显著低于对照组(P0.05)。观察组出院时生存率高于对照组,但差异无统计学意义(P0.05)。结论:与单用乌司他丁相比,醋酸奥曲肽联合乌司他丁治疗重症急性胰腺炎患者疗效更好,其可显著降低患者血清ET、MCP-1、TNF-α和IL-6水平。     

连续性血液净化对重症胰腺炎患者炎症因子、内毒素及肠道黏膜屏障功能的影响

目的:研究连续性血液净化对重症胰腺炎患者炎症因子、内毒素及肠道黏膜屏障功能的影响。方法:选取2014年2月至2015年1月本院收治的86例重症胰腺炎患者,按照投硬币法分为观察组(43例)和对照组(43例)。对照组采取常规治疗,观察组在此基础上加以连续性血液净化治疗。比较两组患者治疗前后炎症因子、内毒素、肠道黏膜屏障功能变化情况,分析两组患者临床症状缓解时间。结果:治疗后,观察组血清白介素-6(IL-6)、白介素-8(IL-8)、白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、内毒素、D-乳酸、二胺氧化酶水平明显低于对照组(P0.05),压痛、腹痛、腹胀症状缓解时间显著短于对照组(P0.05)。结论:连续性血液净化能有效改善重症胰腺炎患者炎症因子水平、内毒素及肠道黏膜屏障功能,促进患者临床症状快速恢复。     

不同剂量乌司他丁对急性胰腺炎患者血清TNF-α，IL-1，IL-6及IL-8水平的影响

目的:探讨不同剂量乌司他丁对急性胰腺炎患者血清TNF-α、IL-1、IL-6、IL-8含量影响。方法:收集我院消化内科收治的急性胰腺炎患者72例,随机分为试验组和对照组,各36例。对照组予以10万IU静点,每8 h 1次;试验组患者予以乌司他丁30万IU静点,每8 h 1次。治疗后比较患者临床症状、血清TNF-α、IL-1、IL-6、IL-8含量、血淀粉酶、肝脏功能以及不良反应发生情况。结果:治疗后,与治疗前相比两组AMY、ALT以及Cr水平均降低(P0.05),血清TNF-α、IL-1、IL-6、IL-8水平均降低(P0.05)。与对照组相比,试验组治疗有效率较高(P0.05);试验组临床症状消失时间、实验室指标恢复时间以及住院天数缩短(P0.05);试验组TNF-α、IL-1、IL-6、IL-8水平较低(P0.05);试验组AMY、ALT以及Cr水平较低(P0.05)。结论:大剂量乌司他丁应用于急性胰腺炎的治疗安全有效,较正常剂量相比,能够缩短治疗时间,降低TNF-α、IL-1、IL-6、IL-8等炎症因子水平。     

8周不同强度运动对大鼠股四头肌HSP70和VEGF的影响

目的:研究不同强度运动的应激条件下,大鼠股四头肌保护性蛋白热休克蛋白70(HSP70)和血管生长因子(VEGF)表达的情况,为运动健身和科学训练提供研究资料。方法:采用任昭君的运动方案模型、参照Bedford及孙晓娟的运动负荷标准。分为四组(n=8):对照组(C组)、小强度运动组(LE组)、中等强度运动组(ME组)和大强度运动组(HE组),每周训练5 d共8周。取大鼠右肢股四头肌组织,采用免疫组织化学技术检测VEGF和HSP70的表达。结果:与对照组相比较,实验各组的股四头肌VEGF蛋白表达明显增加(P<0.01),分别增加了284.8%、63.02%和386.88%,而HSP70蛋白表达分别增加了11.57%、18.54%和3.75%;其中LE组和ME组的HSP70蛋白表达显著增高(P<0.05),HE组的HSP70蛋白表达未出现显著性变化(P<0.05),而ME组比LE组的HSP70蛋白表达增加明显(P<0.05),均高于HE组的HSP70蛋白表达。结论:中低强度运动诱导股四头肌HSP70合成表达,相应的VEGF表达增加;8周小强度有氧递增性运动对大鼠的机体影响非常显著。     

乌司他丁辅助治疗急性重症胰腺炎的疗效及对occludin、CRPI、IL-6水平的影响

目的:研究乌司他丁辅助治疗急性重症胰腺炎的疗效及对咬合蛋白(Occludin)、C反应蛋白(快速)(CRPI)、白细胞介素6(IL-6)水平的影响。方法:选择2014年12月至2016年11月在我院进行治疗的118例急性重症胰腺炎患者,随机将其均分为观察组(59例)和对照组(59例),对照组给予常规治疗,观察组患者则在对照组治疗方案的基础上增加乌司他丁辅助治疗,两组患者的治疗疗程均为两周,记录并比较两组患者治疗前后的血清occludin、CRPI、IL-6水平,治疗后腹胀、腹痛、恶性呕吐及腹膜刺激征等体征消失时间及临床疗效。结果:治疗后,观察组和对照组患者的总有效率分别是88.1%、69.5%,观察组总有效率显著高于对照组(P0.05);观察组患者治疗后的血清occludin水平较对照组患者显著升高(P0.05),血清IL-6及CRPI水平显著低于对照组,且腹胀、腹痛、恶性呕吐、腹膜刺激征等体征消失时间显著短于对照组(P0.05)。结论:乌司他丁辅助治疗重症急性胰腺炎的临床疗效显著优于常规治疗,不仅能改善腹胀、腹痛、恶性呕吐及腹膜刺激征,而且可有效降低患者血清CRPI、IL-6水平和提高occludin水平。     

帕金森病患者血清klk6、HSP70检测的临床意义研究

摘要 目的：探讨帕金森病（PD）患者血清激肽释放酶6（klk6）、热休克蛋白70（HSP70）水平与病情严重程度以及PD轻度认知障碍（PD-MCI）的关系。方法：选择2021年2月至2022年2月徐州医科大学附属医院收治的165例PD患者（PD组），根据修订的Hoehn-Yahr分级将PD患者分为早期组（1.0~2.5级，59例）、中期组（3.0级，65例）和晚期组（4.0~5.0 级，41例），根据是否存在PD-MCI将PD患者分为PD-MCI组（66例）和非PD-MCI组（99例），另选择同期72例于我院门诊体检的健康志愿者为对照组。检测血清klk6、HSP70水平，比较不同分组血清klk6、HSP70水平差异，多因素Logistic回归分析影响PD患者发生PD-MCI的因素。结果：PD组血清klk6水平高于对照组（P＜0.05），HSP70水平低于对照组（P＜0.05），晚期组血清klk6水平高于早期组和中期组，且中期组血清klk6水平均高于早期组（P＜0.05），晚期组血清HSP70水平低于早期组和中期组，且中期组血清HSP70水平低于早期组（P＜0.05）；PD-MCI组血清klk6水平高于非PD-MCI组（P＜0.05），HSP70水平低于非PD-MCI组（P＜0.05）。多因素Logistic回归分析结果显示年龄偏高、修订的Hoehn-Yahr分级晚期、高水平klk6是PD患者发生PD-MCI的危险因素（P＜0.05），高水平HSP70是PD患者发生PD-MCI的保护因素（P＜0.05）。结论：PD患者血清klk6水平增高，HSP70水平降低与修订的Hoehn- Yahr分级增加以及PD-MCI有关，检测血清klk6、HSP70水平有助于评估PD病情以及PD-MCI风险。     

不同剂量瑞舒伐他汀钙治疗脑梗死患者的临床疗效及机制研究

目的:探讨不同剂量瑞舒伐他汀钙治疗脑梗死患者的临床疗效,并分析其疗效机制。方法:将2015年2月至2018年2月医院诊治的脑梗死患者84例按随机数字表法分为观察组(42例)及对照组(42例)。所有患者入院后均给予降血压、降血糖、稳定颅内压以及改善微循环等基本治疗,在此基础上,对照组口服瑞舒伐他汀钙10 mg/d,观察组口服瑞舒伐他汀钙20 mg/d,两组疗程均为14 d。比较两组疗效、治疗前后美国国立卫生研究所卒中量表(NIHSS)评分以及日常生活能力量表(Barthel指数)变化,并比较两组血清白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、高迁移率族蛋白-1(HMGB1)和超敏C反应蛋白(hs-CRP)水平,分析血清HMGB1、IL-6、IL-8和hs-CRP之间的相关性,记录两组患者治疗过程中不良反应发生情况。结果:观察组治疗总有效率为95.24%(40/42),明显高于对照组的80.95%(34/42)(P0.05)。两组治疗后NIHSS评分均低于治疗前,Barthel指数高于治疗前,同时,观察组NIHSS评分降低程度大于对照组,Barthel指数升高程度大于对照组(P0.05)。治疗后两组患者血清IL-6、IL-8、HMGB1、hs-CRP明显降低,且观察组血清IL-6、IL-8、HMGB1、hs-CRP明显低于对照组(P0.05)。Person相关性分析表明患者血清HMGB1与IL-6、IL-8、hs-CRP呈正相关(r=0.306,0.428,0.367,均P0.05),IL-6与IL-8、hs-CRP呈正相关(r=0.327,0.385,P0.05),IL-8与hs-CRP亦呈正相关(r=0.430,P0.05)。治疗期间两组不良反应发生率比较无统计学差异(P0.05)。结论:高剂量瑞舒伐他汀钙治疗脑梗死能够促进神经功能恢复,提高日常生活能力,临床疗效显著,其机制可能与降低脑梗死急性期炎症反应有关。     

PROGRESS STUDY: Progression of chronic kidney disease in children and heat shock proteins

Various molecular and cellular processes are involved in renal fibrosis, such as oxidative stress, inflammation, endothelial cell injury, and apoptosis. Heat shock proteins (HSPs) are implicated in the progression of chronic kidney disease (CKD). Our aim was to evaluate changes in urine and serum HSP levels over time and their relationships with the clinical parameters of CKD in children. In total, 117 children with CKD and 56 healthy children were examined. The CKD group was followed up prospectively for 24 months. Serum and urine HSP27, HSP40, HSP47, HSP60, HSP70, HSP72, and HSP90 levels and serum anti-HSP60 and anti-HSP70 levels were measured by ELISA at baseline, 12 months, and 24 months. The urine levels of all HSPs and the serum levels of HSP40, HSP47, HSP60, HSP70, anti-HSP60, and anti-HSP70 were higher at baseline in the CKD group than in the control group. Over the months, serum HSP47 and HSP60 levels steadily decreased, whereas HSP90 and anti-HSP60 levels steadily increased. Urine HSP levels were elevated in children with CKD; however, with the exception of HSP90, they decreased over time. In conclusion, our study demonstrates that CKD progression is a complicated process that involves HSPs, but they do not predict CKD progression. The protective role of HSPs against CKD may weaken over time, and HSP90 may have a detrimental effect on the disease course.Supplementary InformationThe online version contains supplementary material available at 10.1007/s12192-021-01239-9.     

HSP70 alleviates spinal cord injury by activating the NF-kB pathway

Objectives:Spinal cord injury (SCI) is an acute traumatic lesion of neurons in the spinal cord which has a high prevalence in the world, and has no effective surgical treatment. HSP70 is a molecular chaperone protein, serves a protective role in several different models of nervous system injury. The aim of the present study was to investigate the anti-inflammatory role of HSP70 in spinal cord injury and explore its mechanism.Methods:In vivo and in vitro models were constructed to mimic SCI. The Basso Mouse Scale (BMS) was applied to assess SCI degrees of the mouse model. Immunofluorescence (IF) was used for visualizing HSP70 and Iba1 in the spinal cord. Western blot assay was employed to quantify HSP70 and p65, and ELISA was for IL-1β and TNF-α.Results:The results showed that HSP70 expression decreased after SCI. HSP70 and Iba1 showed a decrease of co-localization in SCI mice. Further studies revealed that p65 was upregulated during the process of SCI. Overexpression of HSP70 inhibited the expression of p65 both in vitro and in vivo, and promoted the recovery of SCI mice.Conclusions:HSP70 was involved in the pathological process of spinal cord injury, HSP70 alleviated the spinal cord injury via inhibiting NF-κB signaling pathway.     

血清IL-8在过敏性紫癜的表达及意义

目的:研究血清白介素-8(IL-8)在过敏性紫癜(HSP)中的表达及意义。方法:采用酶联免疫吸附试验(ELISA)检测和比较过敏性紫癜急性期(包括紫癜性肾炎、非紫癜性肾炎)患儿、过敏性紫癜恢复期患儿、健康体检儿童血清IL-8的含量。结果:过敏性紫癜急性期患儿血清IL-8含量显著高于过敏性紫癜恢复期患儿和健康儿童(P0.05),而过敏性紫癜恢复期、健康儿童血清IL-8含量比较无统计学差异(P0.05)。紫癜性肾炎患儿、非紫癜性肾炎患儿的血清IL-8含量均明显高于健康儿童,且非紫癜性肾炎血清IL-8含量显著低于紫癜性肾炎患儿(P0.05)。结论:急性期过敏性紫癜患儿血清IL-8含量上调,可能参与了过敏性紫癜的发生,并可能与紫癜性肾炎的发生有关。     

Possible involvement of HSP70 in pancreatic cancer cell proliferation after heat exposure and impact on RFA postoperative patient prognosis

PurposeAs an alleviative treatment measured in patients with unresectable advanced pancreatic cancer, radiofrequency ablation (RFA) needed more clinical data to prove its advantages and to explore limitations in its utilization. This study was determined to observe the efficacy of RFA, and to explore its impact on perioperative periphery carcinoma as well as the normal pancreatic tissues.MethodsClinical data of 32 patients with pancreatic cancer accepted RFA surgery were collected. Followed up patients’ pain degree and the changes in serum tumor markers CA19-9 and CA 242 before and after surgery. Ex vivo, gave human pancreatic cancer cell line PANC-1 heat treatment to simulate the heat exposure condition periphery carcinoma was experienced during RFA surgery, and to observe the proliferation rate and HSP70 expression change compared with control group.ResultsOf the 32 patients, 1 died of upper gastrointestinal hemorrhage, and 29 survived for more than 5 months, 2 of which for more than 16 months. The average CA19-9 and CA 242 levels of the patients were significantly decreased in 3 months after surgery (t = 9.873, 5.978, P < 0.001). During in vitro experiments, the proliferation rate of PANC-1 cells after heating was significantly increased, accompanied with the increased HSP70 expression. The addition of HSP70 inhibitor can inhibit the rise of proliferation after heat therapy.ConclusionUtilizing RFA treat patients with unresectable advanced pancreatic cancer, could effectively relieve the pain, decline jaundice, and deduce tumor marker levels significantly. However, it failed to extend the long-term survival rate of the patients significantly. This study found that a higher proliferative rate accompanied with a higher HSP70 expression level were observed on in vitro cultured pancreatic carcinoma cells after heat treatment, which could be altered by HSP70 inhibitor. And these findings indicated that the heat exposure might impact periphery carcinoma during RFA surgery and HSP70 might play an important role in patients’ prognosis.     

HSP86 and HSP84 exhibit cellular specificity of expression and co-precipitate with an HSP70 family member in the murine testis

This study extends to the protein level our previous observations, which had established the stage and cellular specificity of expression of hsp86 and hsp84 in the murine testis in the absence of exogenous stress. Immunoblot analysis was used to demonstrate that HSP86 protein was present throughout testicular development and that its levels increased with the appearance of differentiating germ cells. HSP86 was most abundant in the germ cell population and was present at significantly lower levels in the somatic cells. By contrast, the HSP84 protein was detected in the somatic cells of the testis rather than in germ cells. The steady-state levels of HSP86 and HSP84 paralleled the pattern of the expression of their respective mRNAs, suggesting that regulation at the level of translation was not a major mechanism controlling hsp90 gene expression in testicular cells. Immunoprecipitation analysis revealed that a 70-kDa protein coprecipitated with the HSP86/HSP84 proteins in testicular homogenates. This protein was identified as an HSP70 family member by immunoblot analysis, suggesting that HSP70 and HSP90 family members interact in testicular cells. © 1993Wiley-Liss, Inc.     

Increased serum HSP70 levels are associated with the duration of diabetes

The evolutionary conserved family of heat shock proteins (HSP) is responsible for protecting cells against different types of stress, including oxidative stress. Although the levels of HSPs can be readily measured in blood serum, the levels of HSP70 in patients with different durations of diabetes have not been studied before. We quantified serum HSP70 levels in a healthy control group (n = 36) and two groups of type 2 diabetic patients, defined as newly diagnosed diabetes (n = 36) and patients with diabetes duration of more than 5 years (n = 37). The clinical characteristics and biochemical parameters were evaluated in the studied population. We found that serum HSP70 levels were significantly higher in patients with diabetes when compared with controls (p < 0.001) and it was higher in patients with disease for more than 5 years than in newly diagnosed patients (p < 0.001). Serum HSP70 was inversely correlated with fasting blood sugar in patients with diabetes for more than 5 years (r = −0.500, p = 0.002), positively correlated with the history of hypertension in newly diagnosed patients (p < 0.001), and positively correlated with age in patients with diabetes (r = 0.531, p = 0.001). Serum level of HSP70 is significantly higher in patients with diabetes and correlates with the duration of disease. Higher HSP70 in prolonged diabetes versus newly diagnosed diabetes may be an indicator of metabolic derangement in the course of diabetes.     

双歧三联活菌片与小儿止泻安颗粒对急性腹泻患儿血清IL-7、心肌酶及同工酶水平的影响

目的:探讨双歧三联活菌片联合小儿止泻安颗粒对小儿急性腹泻患儿血清IL-7、心肌酶及同工酶水平的影响。方法:收集我院就诊的120例小儿急性腹泻患者,随机分为实验组和对照组,每组60例。对照组患者给予双歧三联活菌片口服治疗;实验组在对照组基础上给予小儿止泻安颗粒口服治疗。观察并比较两组患者治疗前后血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、乳酸脱氢酶(LDH)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、白介素-7(IL-7)以及临床治疗有效率。结果:与治疗前相比,两组患者治疗后AST、ALT、LDH、CK、CK-MB水平均显著下降,IL-7水平明显升高,差异具有统计学意义(P0.05);与对照组相比,实验组患者的血清AST、ALT、LDH、CK、CK-MB水平较低,IL-7水平较高,差异具有统计学意义(P0.05);与对照组相比,实验组患者的临床治疗有效率较高,差异具有统计学意义(P0.05)。结论:双歧三联活菌片联合小儿止泻安颗粒能够降低小儿急性腹泻患者血清心肌酶、同工酶水平,升高IL-7水平,临床疗效较好。     

卵巢癌患者血清HE4、CA125、CA72-4及炎性因子水平检测的临床价值评估

目的:探讨血清人附睾蛋白4(HE4)、糖类抗原125(CA125)、糖类抗原72-4(CA72-4)及炎性因子IL-6、IL-8、IL-17水平检测对卵巢癌患者的临床价值。方法:选取2015年2月至2017年2月我院收治的卵巢肿瘤患者81例,包括卵巢恶性肿瘤组39例及卵巢良性肿瘤组42例,另选42例健康人作正常对照组,比较各组及临床不同分期卵巢癌患者血清HE4、CA125、CA72-4、IL-6、IL-8、IL-17水平的差异。结果:卵巢恶性肿瘤组血清HE4、CA125、CA72-4、IL-6、IL-8及IL-17水平均显著高于对照组(P0.05),而卵巢良性肿瘤组与对照组以上指标比较差异并无统计学意义(P0.05);随卵巢癌分期增加,患者血清HE4、CA125、CA72-4、IL-6、IL-8及IL-17水平呈上升趋势,以血清HE4、CA125及IL-17增加较为显著(P0.05),而Ⅰ期、Ⅱ期及Ⅲ期卵巢癌患者血清CA72-4、IL-6及IL-8在增加并不明显(P0.05)。结论:卵巢癌患者血清HE4、CA125、CA72-4、IL-6、IL-8及IL-17水平较高,且随临床分期增加呈上升趋势,以上指标可能有助于卵巢癌的诊断、治疗及病情评估。