小骨窗显微手术治疗基底节区高血压脑出血的效果分析及对血清CCCK-18、CTRP-3水平的影响

目的:探讨小骨窗显微手术治疗基底节区高血压脑出血的效果及对血清gaspase切割的细胞角蛋白18(CCCK-18)、补体C1q肿瘤坏死因子相关蛋白3(CTRP-3)水平的影响。方法:选取2016年5月至2018年5月我院收治的160例基底节区高血压脑出血患者,按照随机数表法将其分为观察组(n=82)和对照组(n=78)。对照组采用传统大骨瓣开颅术治疗,观察组采用小骨窗显微手术治疗。观察和比较两组的临床疗效,血肿清除率、术中出血量、术后意识恢复时间、住院时间,治疗前后NIHSS、ADL评分、血清CCCK-18、CTRP-3水平的变化及并发症的发生情况。结果:治疗后,观察组总有效率显著高于对照组[95.12%vs. 79.48%](P0.05);血肿清除率、术中出血量、术后意识恢复时间、住院时间均显著优于对照组[(93.62±3.58)%vs.(85.40±2.19)%,(92.47±12.56)mL vs.(189.25±26.47) mL,(2.01±0.58) d vs.(8.69±2.03) d,(13.39±2.08) d vs.(19.45±3.76) d](P0.05);NIHSS评分显著低于对照组[(9.76±1.42)分vs.(20.57±3.26)分](P0.05);ADL评分显著高于对照组[(86.42±8.64)分vs.(75.39±7.02)分](P0.05);血清CCCK-18水平显著低于对照组[(201.76±32.59) U/L vs.(237.57±39.20) U/L,(29.59±5.19) ng/mL vs.(42.97±7.94)ng/mL](P0.05);CTRP-3水平显著高于对照组[(289.59±35.19)ng/mL vs.(232.97±27.94)ng/mL](P0.05);并发症总发生率显著低于对照组[3.65%(3/82) vs. 14.10%(11/78)](P0.05)。结论:小骨窗显微手术治疗基底节区高血压脑出血的疗效显著,可更有效清除血肿,缓解血肿压迫,改善神经功能,减少继发性损伤,安全性高,可能与其降低血清CCCK-18水平及升高CTRP-3水平有关。     

微创踝关节融合术治疗老年创伤性踝关节炎中的临床效果及对患者氧化损伤与骨代谢的影响

目的:研究微创踝关节融合术治疗老年创伤性踝关节炎中的临床效果及对患者氧化损伤与骨代谢的影响。方法:收集2014年3月至2015年3月我院收治的94例老年创伤性踝关节炎患者,按随机数表法分为实验组和对照组,每组各45例。两组患者在手术前均进行常规检查,对照组采用常规开放式踝关节融合术,实验组采用微创踝关节融合术。对比两组治疗后血清氧化损伤指标肌红蛋白(MYO)、缺血修饰白蛋白(IMA)、总抗氧化能力(TAC)、丙二醛(MDA)水平,骨代谢指标碱性磷酸酶(ALP)、酸性磷酸酶(ACP)、甲状旁腺素(PTH)、骨钙素(BGP)、降钙素(CT)水平,视觉疼痛模拟评分(VAS)、美国矫形外科足踝协会(AOFAS)评分及不良反应的发生情况。结果:治疗后,实验组血清MYO、IMA、MDA水平显著低于对照组[(20.48±2.59)ng/mL vs.(27.07±2.97)ng/m L,(65.68±8.20)U/L vs.(74.27±9.01)U/L,(5.01±1.03)nmol/L vs.(9.64±2.17)nmol/L](P0.05),血清TAC水平显著高于对照组[(11.40±2.50)kU/L vs.(7.36±1.03)kU/L](P0.05);血清ALP、BGP、CT水平均显著高于对照组[(103.28±12.47)U/L vs.(90.53±10.02)U/L,(11.08±1.42)ng/L vs.(8.01±1.23)ng/L,(61.39±5.87)ng/L vs.(50.28±4.92)ng/L](P0.05),ACP、PTH水平均显著低于对照组[(5.21±0.60)U/L vs.(8.03±0.92)U/L,(42.95±5.38)ng/L vs.(60.49±6.92)ng/L](P0.05);VAS评分显著低于对照组[(1.06±0.23)分vs.(3.79±0.67)分](P0.05),AOFAS评分显著高于对照组[(73.02±6.28)分vs.(65.58±5.13)分](P0.05);不良反应总发生率显著低于对照组[6.66%(3/45) vs. 20.41%(10/49)](P0.05)。结论:微创踝关节融合术可调节老年创伤性踝关节炎患者的骨代谢,增强骨密度,减少术后不良反应,有利于改善患者预后。     

舒血宁注射液联合胞磷胆碱钠对脑梗死患者神经功能及血清IGF-1、vWF、HIF-1α水平的影响

目的:研究舒血宁注射液联合胞磷胆碱钠对脑梗死患者神经功能及血清胰岛素样生长因子(IGF-1)、血浆血管性病友因子(v WF)、缺氧诱导因子-1α(HIF-1α)水平的影响。方法:收集2014年3月至2016年3月我院收治的120例脑梗死患者,按抽签法分为对照组和实验组,每组各60例。两组均采用基础治疗,对照组在此基础上采用胞磷胆碱钠治疗,每次2粒,每天3次;实验组在对照组的基础上采用舒血宁注射液治疗,每次5 m L,加入0.9%生理盐水250 m L中混合后进行静脉滴注,每天1次。两组治疗疗程均为2周。对比两组治疗前后神经功能缺损评分(NIHSS)、日常生活能力(ADL),血清IGF-1、v WF、HIF-α水平的变化,不良反应的发生情况及治疗疗效。结果:治疗后,实验组总有效率显著高于对照组[91.66%(55/60) vs. 71.66%(43/60)](P0.05),NIHSS评分显著低于对照组[(7.01±1.23)分vs.(10.52±1.98)分](P0.05),ADL评分显著高于对照组[(68.16±5.62)分vs.(56.20±5.13)分](P0.05);血清IGF-1水平显著高于对照组[(174.29±27.35)IU/mL vs.(152.08±25.29)IU/mL](P0.05),血清v WF、HIF-α水平显著低于对照组[(5.63±1.21)mg/L vs.(11.92±1.35)mg/L,(14.21±3.11)μmol/L vs.(20.16±3.89)μmol/L](P0.05);两组不良反应的发生率比较无显著差异(P0.05)。结论:舒血宁注射液联合胞磷胆碱钠治疗脑梗死的临床疗效显著优于单用胞磷胆碱钠治疗,其可有效改善患者的神经功能,恢复脑组织功能,可能与其效减轻炎症反应及抑制氧化应激反应有关。     

腺苷蛋氨酸联合白蛋白治疗新生儿黄疸的临床疗效及对血清γ-谷氨酰转移酶、胰岛素样生长因子水平的影响

目的:探讨腺苷蛋氨酸联合白蛋白治疗新生儿黄疸的临床疗效及对患儿血清γ-谷氨酰转移酶、胰岛素样生长因子水平的影响。方法:选取2017年3月至2019年3月我院收治的203例新生儿黄疸患儿,按照随机数表法分为观察组(n=105)和对照组(n=98)。对照组采用白蛋白治疗,观察组采用腺苷蛋氨酸联合白蛋白治疗。观察及对比两组治疗疗效,黄疸消退时间及黄疸指数水平,治疗前后胆红素各项指标、γ-谷氨酰转移酶、胰岛素样生长因子、转铁蛋白水平的变化。结果:治疗后,观察组总有效率显著高于对照组[92.38%(97/105) vs. 80.61%(79/98)](P0.05);黄疸消退时间及黄疸指数水平均显著低于对照组[(4.15±0.82) d vs.(5.31±0.92) d,(56.02±7.36)μmol/L vs.(82.86±9.32)μmol/L](P0.05);间接胆红素、结合胆红素、总胆红素水平均显著低于对照组[(108.75±6.21) d vs.(146.03±7.32) d,(7.49±0.85)μmol/L vs.(9.57±1.02)μmol/L,(117.80±6.52)μmol/L vs.(151.09±8.34)μmol/L](P0.05);γ-谷氨酰转移酶、胰岛素样生长因子均显著低于对照组[(56.01±6.45) U/L vs.(89.56±10.73) U/L,(19.30±2.17) ng/L vs 26.78±3.67) ng/L](P0.05);而转铁蛋白水平均显著高于对照组[(1.96±0.27)g/L vs.(1.60±0.24)g/L](P0.05)。结论:腺苷蛋氨酸联合白蛋白治疗新生儿黄疸的临床疗效显著优于单用白蛋白治疗,其可有效改善患儿的黄疸临床症状,降低血清胆红素、γ-谷氨酰转移酶、胰岛素样生长因子水平。     

白芍总苷对糜烂型口腔扁平苔藓外周血TNF-α，IFN-γ及IL-10水平的影响

目的:研究白芍总苷对糜烂型口腔扁平苔藓外周血肿瘤坏死因子(TNF-α)、γ-干扰素(IFN-γ)及白细胞介素-10(IL-10)水平的影响。方法:选取2014年7月至2015年7月我院接诊的糜烂型口腔扁平苔藓患者80例作为本次研究对象。对照组患者采用低浓度他克莫司治疗,观察组采用白芍总苷治疗,观察两组患者治疗前后血清TNF-α、IFN-γ及IL-10水平的变化、糜烂面积、疼痛评分及近期治疗疗效。结果:治疗后,观察组血清TNF-α、IL-10水平显著低于对照组,血清IFN-γ水平明显高于对照组[(2.16±0.61)μg/m L vs(3.04±0.80)μg/m L、(258.93±5.72)ng/L vs(273.41±6.03)ng/L、(319.27±53.46)ng/L vs(290.95±51.03)ng/L(P0.05),糜烂面积、疼痛评分均显著低于对照组[(0.10±0.03)cm2 vs(0.51±0.10)cm~2、(1.01±0.30)分vs(3.20±0.78)分](P0.05),近期治疗疗效优于对照组95.00%(38/40)vs75.00%(30/40)(P0.05)。结论:白芍总苷治疗糜烂型口腔扁平苔藓的效果显著,可能与其调节血清TNF-α、IFN-γ及IL-10的水平有关。     

自体干细胞移植治疗失代偿期肝硬化的疗效及对肝脏储备功能、血清LPS、HGF水平的影响

目的:探讨自体干细胞移植治疗失代偿期肝硬化的疗效及对肝脏储备功能、血清内毒素(LPS)、肝细胞生长因子(HGF)水平的影响。方法:选择2015年9月至2017年9月我院接诊的86例失代偿期肝硬化患者作为本研究对象,通过随机数表法将其分为观察组和对照组,每组43例。对照组给予失代偿期肝硬化常规综合治疗,观察组在对照组基础上进行自体干细胞移植治疗。比较两组治疗前、治疗12周后实验室指标、终末期肝病模型系统评分(MELD)、血清LPS、HGF水平的变化以及不良反应的发生情况。结果:治疗后,两组丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBil)水平均明显低于治疗前,白蛋白(ALB)较治疗前显著升高(P0.05),观察组ALT、AST、TBil均明显低于对照组,ALB水平明显高于对照组[(45.60±4.12)U/L vs.(56.84±6.20)U/L,(57.45±5.01)U/L vs.(68.99±6.84)U/L,(36.53±3.45)g/L vs.(30.42±2.89)g/L,(50.23±4.83)μmol/L vs.(62.30±6.76)μmol/L](P0.05);治疗后,两组MELD评分较治疗前显著降低(P0.05),观察组MELD评分明显低于对照组[(21.89±2.74)分vs(27.84±3.51)分](P0.05);治疗后,两组血清LPS较治疗前显著降低,HGF较治疗前显著升高(P0.05),观察组血清LPS明显低于对照组,HGF明显比对照组高[(0.43±0.05)ng/mLvs(0.60±0.09)ng/mL,(389.56±27.40)pg/mL vs(301.23±22.30)pg/mL](P0.05)。对照组和观察组治疗期间不良反应总发生率分别为8.89%(4/43)、13.95%(6/43),差异无统计学意义(P0.05)。结论:自体干细胞移植治疗失代偿期患者可明显改善肝功能、肝脏储备功能及血清LPS、HGF的表达,且安全性高。     

骨化三醇联合腹膜透析疗法治疗慢性肾功能衰竭的临床疗效及对患者血清ProGRP、Cysc、Chemerin水平的影响

目的:探讨骨化三醇联合腹膜透析疗法治疗慢性肾功能衰竭的临床疗效及对患者血清Pro-Gastrin-Releasing Peptide (ProGRP)、CystatinC(Cysc)、Chemerin水平的影响。方法:选取我院2017年2月至2018年1月收治的98例慢性肾功能衰竭患者,按照随机数表法将其分为观察组(n=51)和对照组(n=47)。对照组采用腹膜透析疗法治疗,观察组采用骨化三醇联合腹膜透析疗法治疗。观察和比较两组治疗前后肾功能指标尿素氮(blood urea nitrogen,BUN)、血清肌酐(Serum creatinine Cr)、24小时尿蛋白(24 h urinary protein,24 h UP),生化指标白蛋白(albumin,Alb)、血红蛋白(hemoglobin,Hb)及红细胞(red blood cell,RBC),胃泌素释放肽前体(ProGRP)、血清胱抑素(Cys C)、趋化素(Chemerin)水平的变化,6个月、1年生存率及不良反应的发生情况。结果:治疗后,观察组BUN、SCr、24hUP水平均显著低于对照组[(13.95±3.06)mmol/L vs.(21.10±3.85)mmol/L,(260.12±40.32)μmol/L vs.(354.93±51.06)μmol/L,(1.75±0.45)g/24 h vs.(2.67±0.80)g/24 h](P0.05);Alb水平显著低于对照组[(27.85±3.58)g/L vs.(33.06±4.27)g/L](P0.05);Hb、RBC显著高于对照组[(91.72±13.46)g/L vs.(82.36±10.15)g/L,(379.47±92.08)×1012/L vs.(315.70±73.24)×1012/L](P0.05);ProGRP、Chemerin水平显著低于对照组[(49.23±4.72)pg/mL vs.(63.87±7.30)pg/mL,(37.02±6.15)μg/L vs.(30.63±4.81)μg/L](P0.05);Cysc水平显著高于对照组[(80.75±16.08)mL/min vs.(98.81±18.07)mL/min](P0.05);6个月、1年生存率均显著高于对照组[96.08%(49/51) vs. 91.49%(43/47),90.20%(46/51) vs. 74.47%(35/47)](P0.05);不良反应总发生率显著低于对照组[17.65%(9/51) vs. 44.68%(21/47)](P0.05)。结论:骨化三醇联合腹膜透析疗法治疗慢性肾功能衰竭的临床效果显著优于单用,其可有效减轻患者的临床症状,纠正电解质紊乱,改善肾功能和预后,可能与降低血清ProGRP、Chemerin水平及提高血清Cysc水平有关。     

鲑鱼降钙素联合糖皮质激素吸入治疗COPD合并骨质疏松的疗效及对血清PINP、β-CTX、MMP-9水平的影响

目的:研究鲑鱼降钙素联合糖皮质激素吸入治疗慢性阻塞性肺疾病(Chronic obstructive pulmonary disease COPD)合并骨质疏松的疗效及对血清Ⅰ型前胶原氨基端肽(Amino terminal peptide of procollagen type I PINP)、β-胶原降解产物(β-Cterminal telopeptide of typeⅠcollagenβ-CTX)、基质金属蛋白酶(matrixmetalloproteinase-9 MMP-9)水平的影响。方法:选取2017年3月至2018年4月我院收治的97例COPD合并骨质疏松患者,按照随机数表法分为观察组(47例)和对照组(50例),两组均采用常规治疗,对照组在此基础上采用糖皮质激素吸入治疗,观察组在对照组的基础上采用鲑鱼降钙素治疗。观察和比较两组患者的疗效,治疗前后肺功能指标(一秒钟用力呼气量(forced expiratory volume in one second FEV1)、第一秒用力呼气容积占用力肺活量百分比(Forced expiratory volume in the first second is the percentage of forced vital capacity FEV1/FVC)、血清PINP、β-CTX、MMP-9水平、腰椎骨密度、股骨颈股密度的变化。结果:治疗后,观察组总有效率显著高于对照组[91.48%(43/47) vs. 70.00%(35/50)](P0.05);FEV1、PO2、FEV1/FVC水平均显著高于对照组[(85.20±13.72)%vs.(70.62±11.37)%,(76.30±8.64)mmhg vs.(62.49±7.13)mmhg,(68.08±11.34)%vs.(60.84±9.75)%](P0.05);PINP、β-CTX、MMP-9水平均显著低于对照组[(17.02±3.67)mg/L vs.(14.86±3.13)mg/L,(93.20±8.01)ng/L vs.(117.39±11.42)ng/L,(47.24±3.75)ng/mL vs.(60.26±4.67)ng/mL](P0.05);腰椎骨密度、股骨颈股密度水平均显著高于对照组[(1.12±0.25)g/cm~2vs.(0.89±0.23)g/cm2,(1.14±0.23)g/cm~2vs.(0.90±0.21)g/cm~2](P0.05)。结论:鲑鱼降钙素联合糖皮质激素吸入治疗COPD合并骨质疏松的疗效显著优于单用糖皮质激素吸入治疗,其能够更有效改善患者肺功能,调节血钙,提高骨密度,缓解骨痛,可能与其降低血清PINP、β-CTX、MMP-9的水平有关。     

微创钻孔引流术对高血压脑出血患者近期疗效及颅内感染、血肿再扩大的影响

目的:研究微创钻孔引流术对高血压脑出血患者近期疗效及颅内感染、血肿再扩大的影响。方法:选取2017年3月至2019年2月的81例高血压脑出血患者。按照随机数表法分为观察组(n=42)和对照组(n=39),对照组采用常规开颅血肿清除术治疗,观察组采用微创钻孔引流术治疗。观察两组治疗疗效情况,血肿量、髓鞘碱性蛋白(MBP)、中枢神经特异蛋白S100水平、NIHSS、GCS评分,不良反应情况。结果:治疗后,观察组总有效率显著高于对照组[92.86%(39/42)vs69.23%(27/39)](P<0.05);血肿量、MBP、S100水平均显著低于对照组[(9.32±2.70)mL vs(15.51±3.01)mL,(3.65±0.52)μg/L vs(4.20±0.71)μg/L,(0.98±0.26)μg/L vs(1.39±0.35)μg/L](P<0.05);NIHSS评分显著低于对照组[(3.90±2.71)分vs(6.34±1.42)分](P<0.05);GCS评分显著高于对照组[(14.84±3.52)分vs(10.69±3.98)分](P<0.05);不良反应总发生率显著低于对照组[9.52%(4/42)vs38.46%(15/39)](P<0.05)。结论:微创钻孔引流术治疗高血压脑出血患者的近期疗效显著,血肿清除彻底,可有效改善脑神经缺损,减少颅内感染,促进预后恢复。     

益气宁心汤联合贝那普利治疗慢性心衰患者的临床疗效及对血清GDF-15、sST2、APN的影响

目的:研究益气宁心汤联合贝那普利治疗慢性心衰患者的临床疗效及对血清生长分化因子-15(GDF-15)、可溶性ST2蛋白(s ST2)、脂联素(APN)的影响。方法:收集2014年3月至2015年3月我院收治的80例慢性心衰患者,按抽签法分为实验组和对照组,每组40例。两组均给予常规基础对症治疗,对照组在此基础上加用贝那普利治疗,每次10 mg,每天1次,实验组在对照组的基础上加用益气宁心汤治疗,每天1剂,于早晚服用,两组治疗疗程均为2周。观察和比较两组的临床疗效及治疗前后心功能左心室舒张末径(LVEDD)、左心室收缩末期内径(LVESD)、左室射血分数(LVEF),GDF-15、s ST2、APN水平、急性生理学与慢性健康状况评分(APACHE-Ⅱ)、肺部感染评分(CPIS)的变化。结果:治疗后,实验组总有效率显著高于对照组[92.50%(37/40)vs72.50%(29/40)](P0.05);LVEDD、LVESD显著低于对照组[(53.56±6.24)mm vs(57.40±6.72)mm,(41.26±4.95)mm vs(46.28±4.86)mm](P0.05),LVEF显著高于对照组[(57.91±5.93)%vs(50.27±5.90)%](P0.05);血清GDF-15、s ST2、APN水平显著低于对照组[(820.81±73.14)ng/L vs(1192.86±93.62)ng/L,(4.28±1.02)ng/mL vs(20.69±3.48)ng/mL,(13.47±2.18)mg/L vs(16.05±3.63)mg/L](P0.05);APACHE-Ⅱ、CPIS评分显著低于对照组[(13.90±3.28)分vs(20.37±4.97)分,(3.24±0.92)分vs(5.38±1.01)分](P0.05)。结论:益气宁心汤联合贝那普利治疗慢性心力衰竭能显著提高其临床疗效,改善心心功能,可能与其有效降低血清GDF-15、s ST2、APN的水平有关。     

慢病毒载体介导GHRH基因在小鼠肌肉组织表达及对动物生长的影响

人与动物体内生长激素受生长激素释放激素(Growth Hormone Releasing Hormone,GHRH)与生长激素抑制激素(Somatostatin,SST)两种因子共同调节,在体内表达外源GHRH,可以提高体内GH基础水平,进而达到促进体内GH释放,加速动物生长的效果.对慢病毒载体系统加以改造,使之成为C...     

Further characteristics of adipokinetic and hyperglycaemic factor(s) of stick insects

When an extract of the corpora cardiaca/corpora allata from two species of wingless stick insects, Carausius morosus and Cuniculina impigra, which cause no adipokinetic or hyperglycaemic effect when injected into the donor insects themselves, is injected into adult Locusta migratoria it resulted in an increase in the haemolymph lipid concentration. The lipid elevation was time dependent, with a maximum effect about 90–180 min after injection, and was also dose-dependent. About 0.001–0.002 (C. morosus) and 0.01 (C. impigra) gland equivalents were needed to produce a significant increase; a maximal effect was reached with approx. 0.075 (C. morosus) and 0.25 (C. impigra) gland equivalents. Carausius extract was also able to elevate carbohydrate concentration in the haemolymph of Periplaneta americana. However, the effect was weak and no maximal response was reached even with a dose of 0.5 gland equivalents. Adipokinetic hormone activity was present in CC/CA extracts of larval Carausius; the activity was about 30 times lower in 1-day-old 2nd instar individuals, and approx. 5 times less at the beginning of the 6th instar than that found in adults. In both stages the hormone levels increased gradually from the beginning to the end of the instar. No age-related changes were observed during the adult stage. Further studies on the lipid-mobilising factor of C. morosus revealed that it was stored entirely in the CC and not in other nervous tissue, e.g. brain, CA, suboesophageal ganglion, thoracic and abdominal cord. The factor was heat stable for at least 1 hr at 100°C and retained its adipokinetic activity after incubation with trypsin and the exopeptidases such as carboxypeptidase A and leucine aminopeptidase. However, activity was abolished when incubated with thermolysin and α-chymotrypsin. From these experiments a close resemblance to the locust AKH, a blocked decapeptide, is suggested.     

养殖条件下3种锦蛇甲状腺相关激素和生长的比较研究

为深入了解人工饲养条件下棕黑锦蛇(Elaphe schrenckii)、赤峰锦蛇(E.anomala)和王锦蛇(E.carinata)生长情况及与甲状腺相关激素的关系,本研究在蛇类非冬眠时期的5月、7月和9月,以尾静脉采血获得3种锦蛇的血清,检测其血清中促甲状腺激素释放激素(TRH)、促甲状腺激素(TSH)和甲状腺素(T4)含量,并记录观察期间3种蛇的体重和体长的增长以及进食量。检测结果,王锦蛇的促甲状腺激素释放激素(TRH)、促甲状腺激素(TSH)和甲状腺素(T4)含量均低于其他2种锦蛇,3种锦蛇促甲状腺激素释放激素(TRH)、促甲状腺激素(TSH)含量最高值均出现在7月份;除了赤峰锦蛇外,棕黑锦蛇和王锦蛇的甲状腺素(T4)含量最高值也出现在7月份,与蛇类快速生长的时间相一致。另外,棕黑锦蛇甲状腺素(T4)含量与进食量的相关系数高于赤峰锦蛇和王锦蛇,而其饲料的转化率也高于后两者,其间存在的关系还需要深入研究。由上述结果可以看出,3种锦蛇促甲状腺激素释放激素(TRH)、促甲状腺激素(TSH)和甲状腺素(T4)含量和变化趋势有着明显的差别,且血清甲状腺素(T4)含量与蛇的进食量、生长和饲料转化率之间有密切的关系。     

Effect of gonadotropin-releasing hormone,stage of sexual maturation and 6-methoxybenzoxazolinone on plasma gonadotropins,ovarian development and uterine weight in prepuberal gilts

Two experiments were conducted with prepuberal gilts at 60, 120 and 160 days of age to a) determine the effect of 6-methoxybenzoxazolinone 6-MBOA) on reproductive plasma hormone concentrations and organ development, and b) determine how plasma follicle-stimulating hormone (FSH) and luteinizing hormone (LH) concentrations before and after injection of gonadotropin-releasing hormone (GnRH) or 6-MBOA varied in relation to ovarian development. In Exp. 1, 12 gilts were used in a 4×4 Latin square design. Four gilts/age group were injected once with: 1) vehicle, 2.5% propylene glycol in 50% ethanol, 2) 2 μg of GnRH/kg body weight (BW), 3) 0.2 mg of 6-MBOA/kg BW, and 4) 2 mg of 6-MBOA/kg BW on four successive days in random order. Blood was collected via indwelling vena cava catheters. Injection of GnRH into gilts increased plasma FSH and LH at each age compared with vehicle (P<0.05). Hormone profiles for FSH and LH differed among age groups (P<0.01), but area under curves did not differ significantly among age groups. Injection of 6-MBOA did not significantly affect plasma FSH and LH. Plasma FSH and LH before the GnRH injection or on days when GnRH was not injected were greater at 60 than at 120 and 160 days (FSH, 128 vs 54 and 42 ng/ml; LH, 0.38 vs 0.16 and 0.13 ng/ml for 60, 120 and 160 days, respectively (P<0.05). In Exp. 2, vehicle, 0.2 or 2 mg of 6-MBOA/kg BW were injected once daily for 7 days in 19 gilts. Injections of 6-MBOA had no detectable effects on gonadotropin secretion, ovarian development or uterine weight. Between 60 and 120 days of age, vesicular follicles developed, ovarian weight increased 20-fold, and uterine weight increased 10-fold (P<0.05); basal concentrations of plasma FSH and LH decreased three- and twofold, respectively.     

Specific binding of an immunoreactive and biologically active 125I-labeled N(1)acylated substance P derivative to parotid cells

A biologically active ¹²⁵I-substance P derivative (I¹²⁵-BH-substance P), prepared by conjugation of substance P with [^125I]Bolton-Hunter reagent, binds specifically to isolated rat parotid cells. The Kd is 4 nM for I-BH-substance P, 5 nM for substance P, 0.18 μM for substance P octa(4–11)peptide, and 1.6 μM for substance P [pyroglutamyl⁶]hexa(6–11)peptide. Substance P free acid and substance P penta(7–11)peptide are much weaker competitors and the C-terminal tri(9–11)peptide has no effect at 30 μM. The binding is also inhibited by 1 μM physalaemin, eledoisin and substance P methyl ester, but not by unrelated peptides. The selective inhibition of the binding by the biologically active analogs and fragments of substance P indicates that the ¹²⁵I-labeled N(1)acylated substance P derivative may interact with a substance P receptor on parotid cells.     

植物愈伤组织培养中内外源激素效应的研究现状与展望

综述了外源激素对愈伤组织诱导、分化的影响,以及内源激素在愈伤组织培养过程中的变化规律。有关愈伤组织培养中外源激素作用的研究日趋细化,而激素变化与适宜继代周期的关系已成为研究热点。随着分子生物学与同位素标记等技术的引入,必将促进愈伤组织培养中激素相关基因调控机理的揭示,以及激素作用位点的精确定位,从而推动植物愈伤组织培养的迅猛发展。     

Induction and regulation of juvenile hormone esterases during the last larval instar of the cabbage looper, Trichoplusia ni

Juvenile hormone esterase titres were monitored in gate I and gate II last instar larvae of Trichoplusia ni using JH III as substrate. Two peaks of activity were observed for both gate I and gate II larvae, although the first and second juvenile hormone esterase peaks for the gate II larvae are extended and delayed one day, respectively. Head or thoracic ligations before the prepupal stage lower or block the appearance of both esterase peaks. Juvenile hormone I and II, as well as homo and dihomo juvenoids can induce the second juvenile hormone esterase peak in both normal and ligated larvae, and increase the esterase titre during the first peak in nonligated larvae. Induction of the juvenile hormone esterases is possible in non-ligated larvae as soon as the moult to the last instar has occurred and in ligated larvae as soon as the first esterase peak has started to decline. Distinct mechanisms of regulation are present for the first and second juvenile hormone esterase peaks. Juvenile hormone does not appear to be involved in regulating its own metabolism by directly inducing the first esterase peak; however, evidence is consistent with a brief burst of juvenile hormone which occurs prior to pupation inducing the production of the second peak of juvenile hormone esterase activity.     

Synthesis and characterization of novel biotinylated carboxyl-terminal parathyroid hormone peptides that specifically crosslink to the CPTH-receptor

Parathyroid hormone (PTH) regulates calcium, phosphorous and skeletal homeostasis via interaction with the G protein-coupled PTH/PTHrP receptor, which is fully activated by the amino-terminal 34 amino-acid portion of the hormone. Recent evidence points to the existence of another class of receptors for PTH that recognize the carboxyl (C)-terminal region of intact PTH (1–84) (CPTHRs) and are highly expressed by osteocytes. Here we report the synthesis and characterization of two novel bifunctional CPTH ligands that include benzoylphenylalanine (Bpa) substitutions near their amino-termini and carboxyl-terminal biotin moieties, as well as a tyrosine³⁴ substitution to enable radioiodination. These peptides are shown to bind to CPTHRs with affinity similar to that of PTH (1–84) and to be specifically and covalently crosslinked to CPTHRs upon exposure to ultraviolet light. Crosslinking to osteocytes or osteoblastic cells generates complexes of 80 and 220 kDa, of which the larger form represents an aggregate that can be resolved into the 80 kDa. The crosslinked products can be further purified using immunoaffinity and avidin-based affinity procedures. While the molecular structure of the CPTHR(s) remains undefined, these bifunctional ligands represent powerful new tools for use in isolating and characterizing CPTHR protein(s).     

The source and action of head factors regulating juvenile hormone esterase in larvae of the cabbage looper, Trichoplusia ni

Brain (median or lateral regions) or suboesophageal ganglion (SOG) homogenates of Day 1 fifth instar larvae of Trichoplusia ni induced the appearance of haemolymph juvenile hormone esterase (JHE) when injected into Day 1, Day 2 or early Day 4 fifth instar ligated hosts. Brain and SOG homogenates of late fourth instars also induced JHE when injected into Day 1 hosts, whole late fifth instar and pupal tissue did not. The pattern of JHE induction by early fourth through Day 3 fifth instar brain and SOG homogenates correlated with natural haemolymph JHE activity occurring at these times. Implantation of late fourth and Day 1 fifth instar brains and/or SOG into similar age hosts similarly induced JHE activity while prothoracic and abdominal ganglia did not. The relative levels of induction following implantation were SOG<brain<brain+SOG. JHE activity which appears in the haemolymph following injection of brain homogenates appears to be largely due to a single enzyme which has an isoelectric point indistinguishable from that of the natural haemolymph enzyme. Evidence is presented which suggests that inhibitory as well as stimulatory brain factors are involved in JHE regulation.     

腹腔镜下卵巢囊肿剥除术前后AMH与bFSH水平的变化与临床价值

目的:对腹腔镜下行卵巢囊肿剥除术患者在手术前和手术后的AMH(anti-mullerian hormone,抗苗勒氏管激素)和bFSH(basic Follicle-stimulating hormone,基础促卵泡刺激素)含量进行测定,分析其临床意义和价值。方法:将2012年1月~2014年1月期间来我院妇产科就诊的34例卵巢巧克力囊肿患者作为研究组,上述患者经相关检查均身体状况符合腹腔镜手术的要求,并选取同期36例非巧囊患者作为对照组,2组患者均实施腹腔镜下卵巢囊肿剥离术进行治疗,并分别在手术前和手术完毕时对患者的AMH和bFSH水平进行测定。结果:研究组与对照组患者在手术完毕后AMH含量[(2.02±2.11,2.77±1.62)ng/m L]同手术前[(2.88±2.30,3.08±1.81)ng/m L]对比具有明显降低,具有统计学差异,(P0.05),研究组患者降低水平更为显著;研究组手术完毕b FSH含量(6.83±1.80)μU/m L明显高于术前(6.29±2.11)μU/m L,具有统计学差异(P0.05),而对照组患者术后血清bFSH含量同术前比较并未明显上升,无统计学差异(P0.05)。结论:行腹腔镜卵巢囊肿剥除术能够使患者的卵巢储备能力下降,测定AMH较b FSH能够更好的反应出卵巢手术后卵巢储备状况。