氟哌噻吨美利曲辛片对伴抑郁情绪非糜烂性胃食管反流病疗效观察

目的探讨氟哌噻吨美利曲辛片治疗非糜烂性胃食管反流病(NERD)合并抑郁的临床效果。方法将我院收治的86例非糜烂性胃食管反流病合并抑郁患者采用随机数字表法进行分组,对照组42例应用埃索美拉唑联合莫沙必利口服,观察组44例在对照组的治疗基础上加用氟哌噻吨美利曲辛片治疗,比较两组患者的临床疗效、抑郁情绪症状体征及生活质量改善情况。结果治疗后,观察组的总有效率为90.91%,明显高于对照组的73.81%,差异有统计学意义(P0.05);RDQ、HAMD及HAMA评分均低于对照组,SF-36评分高于对照组,差异均有统计学意义(P0.05)。结论氟哌噻吨美利曲辛联合常规药物治疗能有效改善非糜烂性胃食管反流病合并抑郁患者的症状体征,消除负面情绪,提升生活质量。     

奥美拉唑联合法莫替丁治疗反流性食管炎的临床疗效及安全性分析

目的分析奥美拉唑联合法莫替丁治疗反流性食管炎的临床疗效及安全性。方法对我院收治的119例反流性食管炎患者按随机数字表法进行分组,对照组59例患者采用奥美拉唑治疗,观察组60例患者给予奥美拉唑联合法莫替丁治疗,比较两组患者的临床治疗效果、症状体征改善情况及不良反应发生率。结果观察组总有效率为96.67%高于对照组的84.75%,差异有统计学意义(P0.05);观察组治疗后的反食、烧灼感、反酸症状体征改善幅度优于对照组,不良反应发生率低于对照组,差异均有统计学意义(P0.05)。结论法莫替丁联合奥美拉唑能有效消除反流性食管炎的症状体征,且安全性高,不良反应低,值得临床应用。     

埃索美拉唑联合胃力康颗粒对胃食管反流的临床疗效及其对患者促甲状腺激素、胃蛋白酶原水平的影响

目的:探讨埃索美拉唑联合胃力康颗粒对胃食管反流的临床疗效及其对患者促甲状腺激素、胃蛋白酶原水平的影响。方法:选取我院老年病科诊断为胃食管反流患者70例,按随机数字表法平均分为两组,对照组35例予以胃力康颗粒治疗,观察组35例在对症治疗基础上联合埃索美拉唑治疗,测定并记录两组治疗前后血清促甲状腺激素(TSH)、胃蛋白酶原(PGⅠ、Ⅱ)水平、反流症状评分,治疗后临床疗效及不良反应的发生情况。结果:观察组治疗有效率显著高于对照组,差异具有统计学意义(P0.05)。与对照组比较,观察组治疗后促甲状腺激素水平较高,PGⅠ水平较高,PGⅡ水平较低,反流症状评分水平较低,差异具有统计学意义(P0.05)。结论:埃索美拉唑联合胃力康颗粒治疗胃食管反流的临床效果显著,且能升高促甲状腺激素及PGⅠ水平,降低PGⅡ水平。     

雷贝拉唑联合莫沙必利治疗反流性食管炎的临床疗效观察

目的:治疗雷贝拉唑联合莫沙必利治疗返流性食管炎的临床疗效。方法:108例反流性食管炎患者随机分成两组,治疗组56例和奥美拉唑对照组52例,治疗组应用雷贝拉唑和莫沙必利,对照组应用奥美拉唑和莫沙必利,观察治疗前后患者的临床症改善和内镜变化。结果:治疗8周,治疗组症状积分明显优于对照组(P<0.05),治疗组疗效明显优于对照组(P<0.05),胃镜检查结果示治疗组食管黏膜愈合情况优于对照组(P<0.5)。结论:雷贝拉唑和莫沙必利联用治疗反流性食管炎临床症状明显改善,联合用药临床疗效优于奥美拉唑。     

腹腔镜胃底折叠术和食管裂孔疝修补术在胃食管反流病患者中的临床应用研究

目的:研究腹腔镜胃底折叠术和食管裂孔疝修补术在胃食管反流病的临床应用效果。方法:选择2009年5月~2016年8月于我院接受腹腔镜胃底折叠术和食管裂孔疝修补术治疗的92例胃食管反流病患者作为研究对象,并根据不同的胃底折叠术分为Nissen组54例和Toupet组38例。其中Nissen组患者采用Nissen360°术式治疗,Toupet组患者采用Toupet270°术式治疗。分别比较两组患者术后恢复情况、术后症状缓解情况、手术满意度以及吞咽困难发生情况。结果:两组患者术后Visick评分等级为Ⅰ~Ⅱ级占比高于术前,而Ⅲ~Ⅳ级占比低于术前,差异均有统计学意义(P0.05)。Nissen组术后症状缓解率为90.74%(49/54),与Toupet组的81.58%(31/38)相比,差异无统计学意义(P0.05)。Nissen组手术满意度为92.59%(50/54),与Toupet组的84.21%(32/38)相比,差异无统计学意义(P0.05)。Nissen组患者术后1个月吞咽困难发生率为46.30%(25/54),高于Toupet组的23.68%(9/38),差异有统计学意义(P0.05);而两组患者术后6个月吞咽困难发生率比较差异无统计学意义(P0.05)。结论:腹腔镜胃底折叠术和食管裂孔疝修补术治疗胃食管反流病具有一定的有效性与安全性,可根据患者具体病情选择Nissen360°术式或Toupet270°术式治疗。     

小儿消化性疾病的胃电图变化及与临床特点和胃镜特征的关联性

目的:分析小儿消化性疾病的胃电图变化及与临床病理特征和胃镜特征的关联性。方法:选取2018年1月至2019年5月我院儿科收治的经胃镜和病理学两种方式诊断为消化性疾病的患儿54例为观察组,另选取无胃肠道疾病的健康儿童40例为对照组。比较两组胃电图参数(频率均值和波幅均值),54例胃电图诊断后纤维胃镜检查结果,分析消化性疾病患儿HP感染与临床病理特征、溃疡面积的关系。结果:各组胃病患者胃电慢波频率均值各不相同(P0.05),三组患者胃电慢波波幅均值相比差异具有统计学意义(P0.05);且浅表性胃炎组、胆汁反流性胃炎组患者胃电慢波频率均值、胃电慢波波幅均显著低于胃溃疡组(P0.05);浅表性胃炎组患者胃电慢波频率均值显著低于胆汁反流性胃炎组(P0.05)。胆汁反流性胃炎组患者胃电慢波波幅显著低于浅表性胃炎组(P0.05)。胃镜检查结果显示,其中浅表性胃炎的诊断符合率较高,达90.00%,胃溃疡符合率为60.71%,胆汁反流性胃炎符合率为83.33%。HP检测结果显示,HP阳性患儿占总例数的77.78%(42/54),HP阴性患儿占总例数的22.22%(12/54);HP阳性组患儿淋巴滤泡形成、胃黏膜萎缩、胃黏膜炎性活动的发生率明显高于HP阴性组,差异具有统计学意义(P0.01);HP阳性组溃疡范围2 cm的患儿比例明显高于HP阴性患儿,差异具有统计学意义(P0.01)。结论:小儿消化性疾病胃电图存在餐后NSWP的下降及节律过缓的上升,胃电图检查和胃镜检查在诊断上有较高的符合率,HP感染科引起胃黏膜组织学改变,可作为小儿消化性疾病的靶向治疗。     

和中平逆方治疗肝胃郁热型反流性食管炎临床研究

目的:观察和中平逆方治疗肝胃郁热型反流性食管炎的临床疗效。方法:选择60例经内镜检查及中医辨证确诊为肝胃郁热型的反流性食管炎患者,分为中药治疗组、西药对照组,每组各30例,均以8周为一疗程,疗程结束后进行疗效比较。结果:两组临床症状疗效,治疗组总有效率为96.67%,对照组为96.66%,两组比较无显著性差异(P>0.05);内镜疗效,治疗组总有效率为73.33%,对照组为73.33%,两组比较无显著性差异(P>0.05);肝胃郁热证候疗效,治疗组总有效率为96.67%,对照组为76.67%,两组比较有显著性差异(P<0.05)。结论:1.和中平逆方对反流性食管炎的症状、食管黏膜炎症改善均有明显疗效;2.和中平逆方对肝胃郁热型反流性食管炎中医证候的改善优于奥美拉唑肠溶胶囊。     

小剂量奥美拉唑用于治疗功能性消化不良的效果分析

目的:分析小剂量奥美拉唑用于治疗功能性消化不良的临床效果。方法:选取我院2009年10月至2011年8月收治的260例功能性消化不良患者,随机分为观察组(148例)和对照组(112例)。观察组患者采取口服小剂量奥美拉唑进行治疗,对照组患者采取口服埃索美啦唑联合多潘立酮进行治疗。观察并比较两组患者的胃部烧灼感、餐后饱胀、上腹痛、嗳气等临床症状的改善情况。结果:经过两周的治疗,两组治疗患者的胃部烧灼感、上腹痛、嗳气及餐后饱胀等功能性消化不良病症的改善情况没有明显差异(P0.05)。结论:小剂量奥美拉唑用于治疗功能性消化不良,其效果与埃索美拉唑联合多潘立酮药物的治疗效果相当,且患者治疗后的不良反应发生率低,可作为临床治疗功能性消化不良的有效药物。     

以胸痛为主要表现的胃食管反流病36例临床分析

目的：探讨以胸痛为主要表现的胃食管反流病的病因、临床特点及诊治方法。方法：选择本院近年来诊治的36例已排除心源性及食管器质性病变,诊断为胃食管反流病的胸痛患者,予以埃索美拉唑20mg,每日两次;吗叮林10mg,每日三次治疗8周,观察其疗效并分别于2周,4周,8周记录症状缓解情况。结果：经抗反流治疗8周后,30例（83.33%）胸痛完全消失,4例（11.11%）明显缓解,2例（5.56%）无效,总有效率达94.44%。胸痛症状在治疗2周后积分下降不明显,而治疗4周,8周后显著改善,与治疗前比较有显著性差异（P〈0.01）。结论：胃食管反流病病因复杂、临床表现多样易误诊漏诊,胃镜检查结合24小时食管动态ph监测,必要时给予质子泵抑制剂试验性治疗可提高本病的确诊率。     

丙泊酚联合七氟烷复合麻醉对小儿麻醉苏醒期躁动评分的影响

目的:探讨丙泊酚联合七氟烷对小儿麻醉苏醒期躁动的临床效果。方法:选择2013年8月-2015年8月在我院接受手术治疗的患儿53例,根据麻醉方法不同,将研究对象分为丙泊酚组(17例)、七氟烷组(19组)和联合组(17例),分别给予丙泊酚,七氟烷以及丙泊酚联合七氟烷进行麻醉并维持。观察并比较各组患儿的手术时间、麻醉时间、清醒时间、躁动发生率以及苏醒期躁动评分。结果:三组患儿的手术时间和麻醉苏醒时间比较,差异均无统计学意义(P0.05);联合组患儿躁动发生率及躁动评分均低于七氟烷组和丙泊酚组,差异具有统计学意义(P0.05);三组患儿恶心、呕吐等不良反应的发生率比较,差异无统计学意义(P0.05)。结论:丙泊酚联合七氟烷可显著改善小儿麻醉苏醒期躁动情况,临床效果显著。     

六种藓类植物茎的比较解剖学研究

通过对6种藓类植物,即褶叶青藓(Brachythecium salebrosum(Web.et Mohr.)B.S.G.)、湿地匐灯藓(Plagiomnium acutum(Lindb.)Kop.)、侧枝匐灯藓(Plagiomnium maximoviczii(Lindb.)Kop.)、大凤尾藓(Fissidensnobilis Griff.)、大羽藓(Thuidium cymbifolium(Doz.et Molk.)B.S.G.)和大灰藓(Hypnum plumaeforme Wils.)嫩茎和老茎的石蜡切片和显微观察发现,同一藓类植株的嫩茎和老茎,茎结构稳定,不同种藓类植物茎横切面具有不同特征.植物体茎横切面形状、表层细胞的层数、细胞大小和细胞壁厚薄、皮层细胞大小和形状、中轴的有无以及比例等特征可以作为藓类植物的分科分类依据之一.     

Cause of Senescence of Nine Sorts of Flowers

The levels of endogenous phytohormones and respiratory rate in nine sorts of flowers such as Cymbidium faberi Rolfe, Nopalxochia ackermannii Kunth and others were investigated both at full bloom and senescence and meanwhile the effect of exogenous phytohormones on prolonging the blossoms and promoting ethylene production were tested. There is a high content of endogenous ethylene in all the long-lived flowere, about 3–16 folds higer than the short-lived ones. There is a high level of ABA at full blooming flowers of short-lived flowers, in which there is no or only some cytokinins in it, but the ratio of CTK (6BA+zeatin)/ABA is smaller(l.7). The endogenous ABA reached a much higher level at senescence in all nine sorts of flowers, so it is reasonable to consider that it is ABA which plays an important role of regulation in controlling flower's senescence. There is a much higher level of GA3 and zeatin in the long-lived flowers which is not demonstrated in the shortlived ones. The respiratory rate is one of the factors controtling the longevity of flowers, but it does not play a decided role. Application of 6BA and zeatin prolongs distinctly orchid’s longevity, however exogenous IAA through the promotive action on ethylene production, evidently extends the longevity of the flowers of the Nopalxochia ackermannii Kunth.     

龙胆科药用植物化学成分的研究现状

龙胆科植物在我国的分布范围很广,且多数为药用植物,其多数种属的药用植物,至今其化学成分尚未被系统研究。综述了目前龙胆科药用植物的化学成分的研究现状及一般提取方法,对近年来发现的环烯醚萜及裂环烯醚萜类化合物进行了总结,为本科药用植物的更深入研究提供了参考。     

The effects of glutamine of the maintenance of embryogenic cultures of Cryptomeria japonica

Summary Embryogenic tissues of sugi (Cryptomeria japonica) were induced on a modified Campbell and Durzan (CD) medium containing 1 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 600 mg l⁻¹ glutamine, and subcultured in the medium of the same composition for over 1 yr. This resulted in a mixed culture of embryogenic and non-embryogenic cells. When embryogenic cells were isolated and cultured independently, their capacity to form embryogenic aggregates was lost. Thus, the non-embryogenic cells present within a mixed culture system were essential to the formation of embryogenic aggregates. When embryogenic tissues were isolated and cultured independently on a high glutamine-containing (2400 mg l⁻¹) medium, dry weights and endogenous levels of glutamine increased, and the tissue could generate a large number of embryogenic aggregates. Amino acid analysis of embryogenic and non-embryogenic cells from the maintenance culture indicated a higher level of glutamine was present in the latter. The high endogenous level of glutamine in the non-embryogenic portion of mixed cell masses may be the supplier of glutamine for maintaining the embryogenic property of the tissues.     

Specificity of action of piperidylcholine derivatives as substrates of cholinesterases of various origin

The review deals with study of enzymologic properties of a novel highly specific acetylcholinesterase substrate, N-(β-acetoxyethyl) piperidinium iodomethylate (“piperidylcholine”), and its 30 derivatives that were tested as effectors of cholinesterases of mammals and various species of Pacific squids. It was proven for the first time that responsible for specificity of action was structure of cyclic ammonium grouping of the alcohol part of molecule of the ester substrate. Analysis of specificity is performed based on enzymatic hydrolysis parameters—activity of catalytic center of cholinesterases and bimolecular constant of the reaction rate that are determined at optimal and low substrate concentrations. Among the specially synthesized group of thioester compounds there is revealed one more highly specific acetylcholinesterase substrate—N-(β-acetoxyethyl) piperidinium.     

真菌类遗传学分析的知识结构教学

本文以认知结构理论为指导,讨论了真菌类遗传分析与高等动植物遗传分析的内在联系,认为利用这种内在联系进行教学可收到好的效果并说明了作者的具体教学过程。 Abstract:In the paper, the relationship between genetic analysis of Fungi and genetic analysis of high animal and plant was discussed.A good results were obtained when we adopted this method in the teaching.     

Photoregulation of seed germination of wild-type and of an aurea-mutant of tomato

Seed germination of an aurea mutant of tomato ( Lycopersicon esculentum Mill.) is promoted by continuous irradiation with red, far-red or long-wavelength far-red (758 nm) light as well as by cyclic irradiations (5 min red or 5 min far-red/25 min darkness). Far-red light applied immediately after each red does not change the germination behaviour. Seed germination of the isogenic wild-type, cv. UC-105, is promoted by continuous and cyclic red light while it is inhibited by continuous and cyclic far-red light and by continious 758 nm irradiation. Far-red irradiation reverses almost completely the promoting effect of red light. The promoting effect (in the aurea mutant) and the inhibitory effect (in the wild-type) of continuous far-red light do not show photon fluence rate dependency above 20 nmol m⁻² s⁻¹. It is concluded that phytochrome controls tomato seed germination throgh low energy responses in both the wild type and the au mutant. The promoting effect of continuous and cyclic far-red light in the au mutant can be attributed to a greater sensitivity to P_fr.     

The mechanism of hatching of eggs of Haemonchus contortus

Fluid collected from hatching eggs of Haemonchus contortus contained a lipase which hydrolysed 2-naphthyl laurate (about 0·7 μmol naphthol freed /h/10⁶ eggs). The fluid also hydrolysed l-leucinamide (about 2·3 μmol leucine freed/h/10⁶ eggs). The fluid when added to normal or heated eggs caused ‘hatching’. ‘Hatching’ also occurred in exsheathing fluid from infective juveniles and in a preparation of pancreatic lipase containing leucine aminopeptidase. A purified mammalian leucine aminopeptidase in combination with several different lipases did not attack egg shells.The ‘spontaneous’ hatching of eggs of H. contortus was strongly inhibited by 1,10-phenanthroline, 10^?3M, and this inhibition was reversed by Zn²⁺. However, the inhibition of ‘hatching’ of eggs in externally applied hatching fluid, or the hydrolysis of leucinamide in hatching fluid was generally less marked.