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1.
M. Höft  R. Verpoorte  E. Beck 《Oecologia》1996,107(2):160-169
The growth of Tabernaemontana pachysiphon (Apocynaceae) plants and the alkaloid content of leaves were investigated in the greenhouse at three levels of nutrient supply under two contrasting water and light regimes. We determined height increment, above-ground biomass production, leaf size, specific leaf weight and the content of the alkaloids apparicine, A2, isovoacangine, tubotaiwine and tubotaiwine-N-oxide. The effects of major controlling factors such as light, water and nutrient supply could be directly correlated with growth and were largely independent of each other. In contrast, leaf-alkaloid contents were influenced by interdependencies among the main factors and individually affected in a synergistic or antagonistic manner which deviated from the effects on growth. The following general trends could be identified with respect to the quantitatively predominant alkaloids apparicine, tubotaiwine and isovoacangine. Increasing nutrient supply had a positive effect on both growth and alkaloid content. Drought increased alkaloid content, but retarded growth. High light intensity lowered alkaloid content but promoted growth. We investigated the relationship between primary production and the production of secondary metabolites with respect to relative and total alkaloid content as well as in relation to the leaves' nitrogen status. Our results showed that under conditions of low nutrient supply, higher proportions of leaf nitrogen were allocated to alkaloids than at moderate or high nutrient supply. Under conditions of drought and low light, all plants allocated almost equal proportions of leaf nitrogen to alkaloids, regardless of fertiliser. Total alkaloid content per plant, however, increased with fertilisation. With respect to the N-allocation strategy, we found no indication of a trade-off between primary production and the production of secondary metabolites in this species. Rather, our results are in accordance with the carbon nutrient balance hypothesis.  相似文献   

2.
Twenty-three alkaloids and five steroids and triterpenes have been isolated and identified from the root bark and stem bark of a Nigerian Tabernaemontana pachysiphon. The following bases have not previously been obtained from this species: isositsirikine, 16-epiisositsirikine, normacusine B, 16-epiaffinine, anhydrovobasindiol, tubotaiwine, ibogaline, isovoacangine, voacamine, lochnericine, 3R-hydroxyconopharyngine, 3S-hydroxyconopharyngine and 11-demethylconoduramine, the last three being new alkaloids. The dimeric indole alkaloids and 3R/S-hydroxyconopharyngine were shown to possess strong antibacterial activity against Gram-positive bacteria and the dimers against Gram-negative bacteria also.  相似文献   

3.
Tabernaemontana alba and Tabernaemontana arborea are Apocynaceae species used in Mexican traditional medicine for which little phytochemical information exists. In this study, preliminary gas chromatography/mass spectrometry analyses of different organs obtained from wild plants of both species identified a total of 10 monoterpenoid indole alkaloids (MIAs) and one simple indole alkaloid, nine of which were reported for the first time in these species. Furthermore, callus cultures were established from T. alba leaf explants and regeneration of whole plants was accomplished via somatic embryogenesis. The anti‐addictive MIAs ibogaine and voacangine were then quantified by gas chromatography with flame ionization detection in wild plants of both species, as well as greenhouse‐grown plants, in vitro‐grown plantlets and embryogenic callus of T. alba. Ibogaine and voacangine were present in most samples taken from the whole plants of both species, with stem and root barks showing the highest concentrations. No alkaloids were detected in callus samples. It was concluded that T. alba and T. arborea are potentially viable sources of ibogaine and voacangine, and that these MIAs can be produced through somatic embryogenesis and whole plant regeneration of T. alba. Approaches to increase MIA yields in whole plants and to achieve alkaloid production directly in cell cultures are discussed.  相似文献   

4.
A cell suspension culture of Tabernaemontana elegans lost its ability to produce alkaloids after a prolonged period of subculture. To determine whether it was still capable of performing the later steps of the alkaloid biosynthetic pathway, the culture was fed with tryptamine and loganin. The precursors and alkaloids were determined in the biomass and in the medium during a growth cycle. In this culture, an increase in the amount of serotonin was found in the biomass after feeding of tryptamine and loganin. Secologanin was detected in small amounts but strictosidine was not. Therefore, a limitation in alkaloid formation in this T. elegans cell line occured in the formation of secologanin from loganin. After feeding of secologanin alone, strictosidine, 10-hydroxy strictosidine, strictosidinic acid and two other indole alkaloids, as yet unidentified, were formed. However, the alkaloids originally produced by this cell line were not found. As the biosynthesis is impaired at several steps, it seems that the loss of productivity is more likely to be to a change on the level of the regulation of the pathway, than due to the loss of the capacity to express an individual biosynthetic gene of the pathway.  相似文献   

5.
Temporal heterogeneity of water supply affects grassland community productivity and it can interact with nutrient level and intraspecific competition. To understand community responses, the responses of individual species to water heterogeneity must be evaluated while considering the interactions of this heterogeneity with nutrient levels and population density. We compared responses of four herbaceous species grown in monocultures to various combinations of water heterogeneity, nutrient level, and population density: two grasses (Cynodon dactylon and Lolium perenne), a forb (Artemisia princeps), and a legume (Trifolium repens). Treatment effects on shoot and root biomass were analyzed. In all four species, shoot biomass was larger under homogeneous than under heterogeneous water supply. Shoot responses of L. perenne tended to be greater at high nutrient levels. Although root biomass was also larger under homogeneous water supply, effects of water heterogeneity on root biomass were not significant in the grasses. Trifolium repens showed marked root responses, particularly at high population density. Although greater shoot and root growth under homogeneous water supply appears to be a general trend among herbaceous species, our results suggested differences among species could be found in the degree of response to water heterogeneity and its interactions with nutrient level and intraspecific competition.  相似文献   

6.
The effects of different nutrient availabilities on growth and biomass partitioning in seedlings from the tropical deciduous forest in Mexico were compared. The tree species studied were Heliocarpus pallidus, a species associated with disturbed parts of the forest, and Caesalpinia eriotachys, Jacquinia pungens and Recchia mexicana, species from mature, undisturbed habitats. The tropical deciduous tree seedlings were grown in pure silica sand for 50 days inside growth chambers under four nutrient regimes; 5, 20, 100 and 200% Long Ashton nutrient solutions. Data showed contrasting responses among species to different nutrient availabilities. Except Jacquinia pungens, all species had increased growth and productivity as nutrient level increased from 5 to 100%; however, no significant differences in these parameters were detected between 100 and 200% in all species. Compared with mature forest species, pioneer species showed higher variations in biomass production, relative growth rate and net assimilation rate. In contrast to mature forest species, root/ shoot ratios in Heliocarpus pallidus were greater and thus showed higher biomass allocation to roots when nutrient supply was limited. This response suggests higher phenotypic plasticity in pioneer species. Species from mature parts of the forest (Caesalpinia eriostachys, Recchia mexicana) showed less dependency on nutrient supply than pioneer species. These responses appear to support observations from studies with temperate plants investigating growth responses to soil fertility.  相似文献   

7.
A cell suspension culture of Tabernaemontana divaricata, that had lost alkaloid production, was still capable of producing a similar pattern of alkaloids as directly after its initiation. When fed with early precursors, such as tryptamine and loganin, 57% of the precursors was converted into indole alkaloids such as strictosidine, vallesamine, O-acetylvallesamine and voaphylline. Apparently most of the cell factory has remained stable during the many years of subculturing. Only an early step of the biosynthesis the flux seems to be diverted to other pathways.  相似文献   

8.
The relative importance of breakdown on the accumulation of indole alkaloids has been determined in suspension cultures of Tabernaemontana divaricata and Catharanthus roseus by the feeding of stable isotope labelled alkaloids. In all cultures a considerable amount of the alkaloid biosynthesized was broken down. The breakdown was found to be dependent on the culture period and the half-life was in the order of several days. The breakdown could not explain the difference between producing and non-producing cultures. Further it was determined that in both cultures the breakdown was due to both biotic and abiotic factors.  相似文献   

9.
Different methods of in vitro culture of Catharanthus roseus provide new sources of plant material for the production of secondary metabolites such as indole alkaloids. Callus, cell suspension, plantlets, and transgenic roots cultured in the bioreactor are used in those experiments. The most promising outcomes include the production of the following indole alkaloids: ajmalicine in unorganised tissue, catharanthine in the leaf and cell culture in the shake flask and airlift bioreactor, and vinblastine in shoots and transformed roots. What is very important, enzymatic coupling of monomeric indole alkaloids, vindoline and catharanthine, is possible to form vinblastine in cell cultures. The method of catharanthine and ajmalicine production in the suspension culture in bioreactors has been successful. In this method, elicitation may be used acting on different metabolic pathways. Also of interest is the method of obtaining arbutin from the callus culture of C. roseus conducted with hydroquinone. The transformed root culture seems to be the most promising for alkaloid production. The genetically transformed roots, obtained by the infection with Agrobacterium rhizogenes, produce higher levels of secondary metabolites than intact plants. Also, whole plants can be regenerated from hairy roots. The content of indole alkaloids in the transformed roots was similar or even higher when compared to the amounts measured in studies of natural roots. The predominant alkaloids in transformed roots are ajmalicine, serpentine, vindoline and catharanthine, found in higher amounts than in untransformed roots. Transformed hairy roots have been also used for encapsulation in calcium alginate to form artificial seeds.  相似文献   

10.
《Phytochemistry》1986,25(7):1783-1784
Four monomeric indole alkaloids have been isolated from the bark of Rauvolfia media. Three of them are the known cabucine, reserpiline and mauiensine; the fourth is a new alkaloid, 12-hydroxymauiensine.  相似文献   

11.
Sanjiang Plain is the largest freshwater marsh in China, where plant zonation along water-level gradients is a common phenomenon. The aim of this experiment was to identify the role of water level and nutrient availability on plant zonation in the plain. Growth and root morphology of three perennial emergent macrophyte species were investigated by growing in two water levels (0.1 and 10.0 cm, relative to soil surface) and in two levels of nutrient supply (0 and 0.5 g slow-release fertilizer per container). In the plain, Carex lasiocarpa typically occurs at low elevations, Glyceria spiculosa at medial elevations, and Deyeuxia angustifolia at high elevations. The relative growth rate was the highest in C. lasiocarpa and the lowest in D. angustifolia in the 10.0-cm water level. Among the three species, only total biomass of D. angustifolia was affected by water level, and decreased with increasing water level. High nutrient supply led to increased total biomass in C. lasiocarpa and G. spiculosa. High water level led to an increased root diameter in G. spiculosa and a decreased root length in C. lasiocarpa. In the 10.0-cm water level, low nutrient supply led to thinner roots in D. angustifolia, but resulted in an increased specific root length (SRL) in C. lasiocarpa and root diameter in G. spiculosa. Water-level effect on root porosity was only observed in G. spiculosa, and nutrient amendment did not influence root porosity in all the species. These data indicate that both nutrient and water level are important factors regulating plant distribution pattern in the Sanjiang Plain, because both C. lasiocarpa and G. spiculosa are relatively sensitive to nutrient supply whereas D. angustifolia is sensitive to water level. Handling editor: S. M. Thomaz  相似文献   

12.
Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to an increased proportion of linolenic acid in the main lipid classes. The modifications in lipid composition were obviously necessary for the roots to retain the proper cell membrane fluidity at each temperature. Despite of changes in membrane lipids, no effect on the distribution of indole alkaloids between the roots and the medium could be detected. Instead, the level of alkaloid accumulation showed a clear increase with lowering temperature.Abbreviations PC phosphatidylcholine - PE phosphatidylethanolamine - PI phosphatidylinositol - PS phosphatidylserine - PG phosphatidylglycerol - CL cardiolipin - DGD digalactosyldiglyceride - MGD monogalactosyldiglyceride - NL neutral lipids - DU degree of fatty acid unsaturation  相似文献   

13.
The effects of light intensity, mineral nutrients and seasonal climatic changes on the alkaloid contents of Rauvolfia yunnanensis Tsiang were studied and the experimental results were summarized as follows: The alkaloid contents of Rauvolfia roots increased with the light intensity decreased, and a maximum was reached at about 28% of the normal day light. The supply of the mineral nutrients of P, K especially N would raise the root production and alkaloid contents respectively. Determinations were carried out about the variations of alkaloid contents of the roots, stems and leaves on the samples collected every month of the year from both the wild and the cultivated Rauvolfia. The alkaloid contents in the roots of both wild and cultivated Rauvolfia appeared with only one high peak-August to September (end of the raining season), and a period with low contents appeared from March to May (dry season). The alkaloid contents in stems appeared with two high peaks, one was in coincidence with that of the roots, the other appeared between March and May (dry season) just as the period with low alkaloid contents in root. The alkaloid contents of leaves of wild Rauvolfia appeared with two high peaks, both were the same as those of the stems, but in the cultivated Rauvolfia, only one peak with high contents from July to August (raining season) was observed.  相似文献   

14.
Both water level and nutrient availability are important factors influencing the growth of wetland plants. Increased nutrient supply might counteract the negative effects of flooding on the growth of the fast-growing species. Experimental evidence is scarce and the mechanism is far from clear. The aim of this study is to identify the role of nutrient availability in acclimation to high-water level by investigating the growth and root morphology of the marsh plant Deyeuxia angustifolia, one of the dominant species in the Sanjiang Plain, China. Experimental treatments included two water levels (0 and 10 cm, relative to soil surface) and three levels of nutrient supply (0, 0.5 and 1 g fertilizer per container). High-water level usually led to decreased biomass accumulation, shoot mass and root mass, whereas biomass accumulation was unaffected by water level at the highest nutrient level, indicating that high-nutrient availability played a role in compensating for the growth loss induced by the high-water level. Increased nutrient supply led to decreased root length in 0 cm water-level treatments, but root length increased with nutrient supply in the 10 cm water-level treatments. High-water level usually led to a lower lateral root density, lateral root:main root length ratio and the diameter of main roots and laterals, whereas increased nutrient supply resulted in thicker main roots or laterals, and a higher total root length, lateral root density and lateral root:main root length ratio. These data indicate that the growth of D. angustifolia is restrained by high-water level, and that increased nutrient supply not only ameliorates root characteristics to acclimate to high-water level but also results in a high-total root length to facilitate nutrient acquisition.  相似文献   

15.
Perakine reductase (PR) catalyzes an NADPH-dependent step in a side-branch of the 10-step biosynthetic pathway of the alkaloid ajmaline. The enzyme was cloned by a “reverse-genetic” approach from cell suspension cultures of the plant Rauvolfia serpentina (Apocynaceae) and functionally expressed in Escherichia coli as the N-terminal His6-tagged protein. PR displays a broad substrate acceptance, converting 16 out of 28 tested compounds with reducible carbonyl function which belong to three substrate groups: benzaldehyde, cinnamic aldehyde derivatives and monoterpenoid indole alkaloids. The enzyme has an extraordinary selectivity in the group of alkaloids. Sequence alignments define PR as a new member of the aldo-keto reductase (AKR) super family, exhibiting the conserved catalytic tetrad Asp52, Tyr57, Lys84, His126. Site-directed mutagenesis of each of these functional residues to an alanine residue results in >97.8% loss of enzyme activity, in compounds of each substrate group. PR represents the first example of the large AKR-family which is involved in the biosynthesis of plant monoterpenoid indole alkaloids. In addition to a new esterase, PR significantly extends the Rauvolfia alkaloid network to the novel group of peraksine alkaloids. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. The nucleotide sequences reported in this article have been submitted to the Gene Bank under Accession No: AY766462.  相似文献   

16.
Summary Hairy roots of Catharanthus roseus were cultivated in three different types of bioreactors. The best growth and indole alkaloid production was achieved in an airsparged bioreactor with no other mixing. In the stirred bioreactor or in the bioreactor with medium circulation the roots did not grow, suggesting that hairy roots of C. roseus are more sensitive to stress than root cultures of many other plant species.  相似文献   

17.
The use of fossil fuel is predicted to cause an increase of the atmospheric CO2 concentration, which will affect the global pattern of temperature and precipitation. It is therefore essential to incorporate effects of temperature and water supply on the carbon requirement for root respiration of plants to predict effects of elevated [CO2] on the carbon budget of natural and managed systems.There is insufficient information to support the contentention that an increase in the concentration of CO2 in the atmosphere will enhance the CO2 concentration in the soil to an extent that is likely to affect root respiration. Moreover, there is no convincing evidence for a direct effect of elevated atmospheric [CO2] on the rate of root respiration per unit root mass or the fraction of carbon required for root respiration. However, there are likely to be indirect effects of elevated [CO2] on the carbon requirement of plants in natural systems.Firstly, it is very likely that the carbon requirement of root respiration relative to that fixed in photosynthesis will increase when elevated [CO2] induces a decrease in nutrient status of the plants. Although earlier papers have emphasized that elevated [CO2] favours investment of biomass in roots relative to that in leaves, these are in fact indirect effects. The increase in root weight ratio is due to the more rapid depletion of nutrients in the root environment as a consequence of enhanced growth. This will decrease the specific rate of root respiration, but increase the carbon requirement as a fraction of the carbon fixed in photosynthesis. It is likely that these effects will be minor in systems where the nutrient supply is very high, e.g. in many managed arable systems, and increase with decreasing soil fertility, i.e. in many natural systems.Secondly, a decrease in rainfall in some parts of the world may cause a shortage in water supply which favours the carbon partitioning to roots. Water stress is likely to reduce rates of root respiration per unit root mass, but enhance the fraction of total assimilates required for root respiration, due to greater allocation of biomass to roots.Increased temperatures are unlikely to affect the specific rate of root respiration in all species. Broadly generalized, the effect of temperature on biomass allocation is that the relative investment of biomass in roots is lowest at a certain optimum temperature and increases at both higher and lower temperatures. The root respiration of some species acclimates to growth temperature, so that the effect of global temperature rise is entirely accounted for by the effect of temperature on biomass allocation. The specific rate of root respiration of other species will increase with global warming. In response to global warming the carbon requirement of roots is likely to decrease in temperate regions, when temperatures are suboptimal for the roots' capacity to acquire water. Here global warming will induce a smaller biomass allocation to the roots. Conversely, the carbon requirements are more likely to increase in mediterranean environments, where temperatures are often supraoptimal and a rise in temperature will induce greater allocation of biomass to the roots.  相似文献   

18.
California poppy (Eschscholzia californica Cham.) root cultures produce a variety of benzophenanthridine alkaloids, such as sanguinarine, chelirubine and macarpine, with potent biological activity. Sense and antisense constructs of genes encoding the berberine bridge enzyme (BBE) were introduced into California poppy root cultures. Transgenic roots expressing BBE from opium poppy (Papaver somniferum L.) displayed higher levels of BBE mRNA, protein and enzyme activity, and increased accumulation of benzophenanthridine alkaloids compared to control roots transformed with a -glucuronidase gene. In contrast, roots transformed with an antisense-BBE construct from California poppy had lower levels of BBE mRNA and enzyme activity, and reduced benzophenanthridine alkaloid accumulation, relative to controls. Pathway intermediates were not detected in any transgenic root lines. Suppression of benzophenanthridine alkaloid biosynthesis using antisense-BBE also reduced the growth rate of the root cultures. Two-dimensional 1H-NMR spectroscopy showed no difference in the abundance of carbohydrate metabolites in the various transgenic roots lines. However, transformed roots with low levels of benzophenanthridine alkaloids contained larger cellular pools of certain amino acids compared to controls. In contrast, cellular pools of several amino acids were reduced in transgenic roots with elevated benzophenanthridine alkaloid levels relative to controls. The relative abundance of tyrosine, from which benzophenanthridine alkaloids are derived, was only marginally altered in all transgenic root lines; thus, altering metabolic flux through benzophenanthridine alkaloid pathways can affect cellular pools of specific amino acids. Consideration of such interactions is important for the design of metabolic engineering strategies that target benzophenanthridine alkaloid biosynthesis.  相似文献   

19.
Changes in growth parameters, carbon assimilation efficiency, and utilization of 14CO2 assimilate into alkaloids in plant parts were investigated at whole plant level by treatment of Catharanthus roseus with gibberellic acid (GA). Application of GA (1 000 g m−3) resulted in changes in leaf morphology, increase in stem elongation, leaf and internode length, plant height, and decrease in biomass content. Phenotypic changes were accompanied by decrease in contents of chlorophylls and in photosynthetic capacity. GA application resulted in higher % of total alkaloids accumulated in leaf, stem, and root. GA treatment produced negative phenotypic response in total biomass production but positive response in content of total alkaloids in leaf, stem, and roots. 14C assimilate partitioning revealed that 14C distribution in leaf, stem, and root of treated plants was higher than in untreated and variations were observed in contents of metabolites as sugars, amino acids, and organic acids. Capacity to utilize current fixed 14C derived assimilates for alkaloid production was high in leaves but low in roots of treated plants despite higher content of 14C metabolites such as sugars, amino acids, and organic acids. In spite of higher availability of metabolites, their utilization into alkaloid production is low in GA-treated roots.  相似文献   

20.
Several species from the Apocynaceae family, such as Tabernanthe iboga, Voacanga africana, and many Tabernaemontana species, produce ibogan type alkaloids, some of which present antiaddictive properties. In this study, we used gas chromatography/mass spectrometry (GC/MS) to examine the efficiency of methanol, acetone, ethyl acetate, dichloromethane, chloroform, and hydrochloric acid in extracting the antiaddictive compounds coronaridine, ibogamine, voacangine, and ibogaine (altogether the CIVI‐complex) from the root barks of Tabernaemontana alba and Tabernaemontana arborea. These Mexican species have recently shown great potential as alternative natural sources of the aforementioned substances. Methanol proved to be the most suitable solvent. Furthermore, the crude methanolic extracts could be engaged in a one‐step demethoxycarbonylation process that converted coronaridine and voacangine directly into its non‐carboxylic counterparts ibogamine and ibogaine, respectively, without the intermediacy of their carboxylic acids. The established protocol straightforwardly simplifies the alkaloid mixture from four to two majority compounds. In summary, our findings facilitate and improve both the qualitative and quantitative analysis of CIVI‐complex‐containing plant material, as well as outlining a viable method for the bulk production of these scientifically and pharmaceutically important substances from Mexican Tabernaemontana species.  相似文献   

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