共查询到20条相似文献,搜索用时 46 毫秒
1.
Highlights
· Congenital human cytomegalovirus (CMV) infection causes severe neuropathogenesis.
· Murine CMV failed to break through the placental barrier to transmit to fetus.
· Zhou et al. established a novel mouse system to model congenital HCMV infection.
· The mouse CMV system by Zhou et al can be used for drug screening. 相似文献
· Congenital human cytomegalovirus (CMV) infection causes severe neuropathogenesis.
· Murine CMV failed to break through the placental barrier to transmit to fetus.
· Zhou et al. established a novel mouse system to model congenital HCMV infection.
· The mouse CMV system by Zhou et al can be used for drug screening. 相似文献
2.
Mengyuan Chen Shuizhen He Hualong Xiong Dongxu Zhang Shaojuan Wang Wangheng Hou Xiaomei Zhu Jin Wang Yang Huang Congming Hong Yubin Wu Ruoyao Qi Tianying Zhang Quan Yuan Tingdong Li Yixin Chen Shiyin Zhang Shengxiang Ge Jun Zhang Ningshao Xia 《中国病毒学》2022,37(5):758-761
Highlights
1 A peptide Spep-1 targeting S2 of SARS-CoV-2 spike protein was selected by PhIP-Seq.
2 Spep-1 showed nanomolar affinity and high specificity to spike protein.
3 S-1 based immunoassay can detect femtomolar spike antigen in spiked serum samples.
4 Spep-1 can be used in future on S2 recognition, virus tracing and drug delivery. 相似文献
1 A peptide Spep-1 targeting S2 of SARS-CoV-2 spike protein was selected by PhIP-Seq.
2 Spep-1 showed nanomolar affinity and high specificity to spike protein.
3 S-1 based immunoassay can detect femtomolar spike antigen in spiked serum samples.
4 Spep-1 can be used in future on S2 recognition, virus tracing and drug delivery. 相似文献
3.
Identification of a novel hepacivirus in Mongolian gerbil (Meriones unguiculatus) from Shaanxi,China
Cui-hong An Juan Li Yi-ting Wang Shou-min Nie Wen-hui Chang Hong Zhou Lin Xu Yang-xin Sun Wei-feng Shi Ci-xiu Li 《中国病毒学》2022,37(2):307-310
Highlights:
1. The first hepacivirus detected in Mongolian gerbils from a plague zones in China.
2. A novel hepacivirus closely related to hepacivirus E and F.
3. Mongolian gerbils could be a potential animal model for hepacivirus pathogenicity.
4. Extending the genetic diversity and host range of hepaciviruses. 相似文献
1. The first hepacivirus detected in Mongolian gerbils from a plague zones in China.
2. A novel hepacivirus closely related to hepacivirus E and F.
3. Mongolian gerbils could be a potential animal model for hepacivirus pathogenicity.
4. Extending the genetic diversity and host range of hepaciviruses. 相似文献
4.
Cheng Zhang Zhong-Yi Wang Huan Cui Li-Gong Chen Chun-Mao Zhang Zhao-Liang Chen Shi-Shan Dong Kui Zhao Ying-Ying Fu Ju-Xiang Liu Zhen-Dong Guo 《中国病毒学》2023,38(1):157-161
Highlights
1. H5N8 viruses emerged in the wild bird habitat at Yishui Lake.
2. The homology between HG12 and a Russian human strain was over 99%.
3. HG12 can be transmitted through direct contact between guinea pigs. 相似文献
1. H5N8 viruses emerged in the wild bird habitat at Yishui Lake.
2. The homology between HG12 and a Russian human strain was over 99%.
3. HG12 can be transmitted through direct contact between guinea pigs. 相似文献
5.
Jiaming Li Yidun Zhang Lina Jiang Hongliang Cheng Jingjing Li Li Li Zehui Chen Fei Tang Yingying Fu Yifei Jin Bing Lu Jing Zheng Zhongyi Wang 《中国病毒学》2022,37(5):762-764
Highlights
1 Aerosol emission rates of Delta or Omicron patients were similar.
2 Viral loads in upper respiratory tract of Alpha, Delta and Omicron patients were similar.
3 Viral loads in upper respiratory tract of vaccinated or unvaccinated Delta patients had no difference. 相似文献
1 Aerosol emission rates of Delta or Omicron patients were similar.
2 Viral loads in upper respiratory tract of Alpha, Delta and Omicron patients were similar.
3 Viral loads in upper respiratory tract of vaccinated or unvaccinated Delta patients had no difference. 相似文献
6.
Wenming Jiang Xin Yin Shuo Liu Shaobo Liang Cheng Peng Guangyu Hou Jinping Li Xiaohui Yu Yang Li Jingjing Wang Hualei Liu 《中国病毒学》2022,37(4):631-633
Highlights
1. 13 strains of H7N9 viruses from laying hens in 2020 and 2021 were identified.
2. H7N9 viruses in China comprised at least 11 genotypes.
3. H7N9 viruses are high pathogenic in chickens, not in ducks.
4. The most H7N9 viruses cross-reacted poorly with H7-Re3 antiserum.
5. The H7-Re3 vaccine was unable to prevent H7N9 infection. 相似文献
1. 13 strains of H7N9 viruses from laying hens in 2020 and 2021 were identified.
2. H7N9 viruses in China comprised at least 11 genotypes.
3. H7N9 viruses are high pathogenic in chickens, not in ducks.
4. The most H7N9 viruses cross-reacted poorly with H7-Re3 antiserum.
5. The H7-Re3 vaccine was unable to prevent H7N9 infection. 相似文献
7.
Ruibin Qi Chunchun Meng Jie Zhu Hang Li Qiuhong Miao Jingyu Tang Aoxing Tang Hongyuan Guo Chuncao Liu Chuanfeng Li Zongyan Chen Fang Wang Qinwen Zhang Guangqing Liu 《中国病毒学》2022,37(4):623-626
Highlights:
1. We identified one RHD case caused by a new RHDV variant (GI.2) in China through HA, TEM, and genome sequencing.
2. This is the first study to demonstrate that GI.2 can replicate efficiently in the reproductive system.
3. Our evidence suggests that GI.2 might be introduced into China by contaminated rabbit semen. 相似文献
1. We identified one RHD case caused by a new RHDV variant (GI.2) in China through HA, TEM, and genome sequencing.
2. This is the first study to demonstrate that GI.2 can replicate efficiently in the reproductive system.
3. Our evidence suggests that GI.2 might be introduced into China by contaminated rabbit semen. 相似文献
8.
Yuanzhi Chen Chenguang Shen Jing Chen Junyu Chen Fentian Chen Limin Zhang Xue Liu Siyuan Chen Sen Xue Yongliang Liu Jixian Tang Quan Yuan Yixin Chen Wenxin Luo Ningshao Xia 《中国病毒学》2022,37(4):619-622
Highlights
1. Class-switch recombination was mimicked in hybridomas through a controllable expression system of activation-induced cytidine deaminase.
2. IgG antibodies were generated through this system in an anti-Flu B IgM hybridoma 7G1.
3. IgG1 and IgG2a subtypes of 7G1 present improved antiviral activity in vitro and in vivo. 相似文献
1. Class-switch recombination was mimicked in hybridomas through a controllable expression system of activation-induced cytidine deaminase.
2. IgG antibodies were generated through this system in an anti-Flu B IgM hybridoma 7G1.
3. IgG1 and IgG2a subtypes of 7G1 present improved antiviral activity in vitro and in vivo. 相似文献
9.
Highlights
1 A stable EV-A71 virus vector was created to generate chimeric enterovirus strains expressing capsid protein genes of EV-A71 subgenogroup C5 and CA16.
2 Phenotypic and genetic stability of the generated chimeric EV-A71 and CA16 were analyzed.
3 The amino acids at the cleavage site between VP1 and 2A is crucial for stability. 相似文献
1 A stable EV-A71 virus vector was created to generate chimeric enterovirus strains expressing capsid protein genes of EV-A71 subgenogroup C5 and CA16.
2 Phenotypic and genetic stability of the generated chimeric EV-A71 and CA16 were analyzed.
3 The amino acids at the cleavage site between VP1 and 2A is crucial for stability. 相似文献
10.
Yuchang Li Yanhong Gao Ying Tang Jing Li Sen Zhang Tao Jiang Xiaoping Kang 《中国病毒学》2023,38(1):165-170
Highlights
1. Two RPA methods (F-RPA and VF- RPA) have been developed for monkeypox virus rapid detection.
2. The limit of detection was 15.32 copies/μL for F-RPA and 8.53 copies/μL for VF-RPA,
3. No cross-reaction was found in 14 rash and fever-associated viruses.
4. The two RPA methods developed are simple and rapid, making them potentially useful for detection of MPXV in the field/clinic. 相似文献
1. Two RPA methods (F-RPA and VF- RPA) have been developed for monkeypox virus rapid detection.
2. The limit of detection was 15.32 copies/μL for F-RPA and 8.53 copies/μL for VF-RPA,
3. No cross-reaction was found in 14 rash and fever-associated viruses.
4. The two RPA methods developed are simple and rapid, making them potentially useful for detection of MPXV in the field/clinic. 相似文献
11.
Lei Yang Yingshan Chen Sha Li Yuan Zhou Yongli Zhang Rongjuan Pei Xinwen Chen Yun Wang 《中国病毒学》2023,38(1):162-164
Highlights
1. The first study describing the cross-reactivity of antibodies elicited by a Chinese smallpox vaccine against monkeypox virus.
2. Mice immunized with vaccinia virus Tiantan strain yield antibodies cross-reactive with monkeypox virus protective antigens.
3. Cross-reactivities of VTT-elicited antibodies against monkeypox protective antigens are ranging from 33% to 94%. 相似文献
1. The first study describing the cross-reactivity of antibodies elicited by a Chinese smallpox vaccine against monkeypox virus.
2. Mice immunized with vaccinia virus Tiantan strain yield antibodies cross-reactive with monkeypox virus protective antigens.
3. Cross-reactivities of VTT-elicited antibodies against monkeypox protective antigens are ranging from 33% to 94%. 相似文献
12.
Rongsong Du Ru Xu Jieting Huang Hao Wang Min Wang Qiao Liao Zhengang Shan Huishan Zhong Yourong Zheng Xia Rong Yongshui Fu 《中国病毒学》2022,37(5):765-768
Highlights
1. Currently, HCV 6a has replaced 1b as the most prevalent subtype in blood donors in Guangdong.
2. HCV 6a was the predominant subtype in males and older donors, while 1b predominated in females and younger donors.
3. HCV 6a may expand from Guangdong to other districts of China, and is worthy of attention 相似文献
1. Currently, HCV 6a has replaced 1b as the most prevalent subtype in blood donors in Guangdong.
2. HCV 6a was the predominant subtype in males and older donors, while 1b predominated in females and younger donors.
3. HCV 6a may expand from Guangdong to other districts of China, and is worthy of attention 相似文献
13.
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14.
Highlights
1. The structure of glycoprotein Gc, responsible for mediating membrane fusion between cell and CCHFV, is revealed, but many more mysteries remain.
2. Why do only antibodies against Gc have neutralizing effect, but not the one against Gn?
3. Why can NAbs against Gc only be protective in the animals in preventive settings, but not in the therapeutic administration? 相似文献
1. The structure of glycoprotein Gc, responsible for mediating membrane fusion between cell and CCHFV, is revealed, but many more mysteries remain.
2. Why do only antibodies against Gc have neutralizing effect, but not the one against Gn?
3. Why can NAbs against Gc only be protective in the animals in preventive settings, but not in the therapeutic administration? 相似文献
15.
Moiz Ahmed Khan Kehkashan Imtiaz Humaira Shafaq Joveria Farooqi Mohammad Hassan Afia Zafar Maureen T. Long Kelli L. Barr Erum Khan 《中国病毒学》2022,37(5):774-777
Highlights:
1 Potential transfusion-related transmission of DENV, WNV and JEV was investigated in healthy blood donors from the blood bank of Aga Khan University during July to December 2018.
2 ELISA for DENV, WNV and JEV IgM antibodies and RT-PCR for viral RNA detection were performed.
3 Of the 360 blood donors screened, IgM antibodies for DENV and WNV were positive in 3.9% and JEV in 0.28% respectively while none of the blood donors tested positive for RT-PCR.
4 Majority of the seropositive donors were between 19 to 30 years of age and residents of urban areas, mainly from Karachi City.
5 DENV and WNV seropositivity were significantly associated with residence in Malir District of Karachi. 相似文献
1 Potential transfusion-related transmission of DENV, WNV and JEV was investigated in healthy blood donors from the blood bank of Aga Khan University during July to December 2018.
2 ELISA for DENV, WNV and JEV IgM antibodies and RT-PCR for viral RNA detection were performed.
3 Of the 360 blood donors screened, IgM antibodies for DENV and WNV were positive in 3.9% and JEV in 0.28% respectively while none of the blood donors tested positive for RT-PCR.
4 Majority of the seropositive donors were between 19 to 30 years of age and residents of urban areas, mainly from Karachi City.
5 DENV and WNV seropositivity were significantly associated with residence in Malir District of Karachi. 相似文献
16.
Shuaiyong Wang Manzhu Wang Lingxue Yu Juan Wang Jiecong Yan Xinli Rong Yanjun Zhou Tongling Shan Wu Tong Guoxin Li Hao Zheng Guangzhi Tong Hai Yu 《中国病毒学》2022,37(4):627-630
Highlights
1. Identification of a reassortant EA H1N1 SIV (SD/18) which isolated from a pig farm in Shandong, north China.
2. Phylogenetic analysis showed that SD/18 virus containing a complete internal gene cassette from pdm/09 virus.
3. The results of pathogenicity in mice showed that the mortality rate of SD/18 virus in mice could reach 100%.
4. The potential risk of EA lineage SIVs to humans is very high and we need to pay enough attention to the different reassortant EA H1N1 viruses. 相似文献
1. Identification of a reassortant EA H1N1 SIV (SD/18) which isolated from a pig farm in Shandong, north China.
2. Phylogenetic analysis showed that SD/18 virus containing a complete internal gene cassette from pdm/09 virus.
3. The results of pathogenicity in mice showed that the mortality rate of SD/18 virus in mice could reach 100%.
4. The potential risk of EA lineage SIVs to humans is very high and we need to pay enough attention to the different reassortant EA H1N1 viruses. 相似文献
17.
Reaction of FcCCo3(CO)9 with 2,3-bis(diphenylphosphino)maleic anhydride (bma). X-ray diffraction structure and redox properties of
The reaction between the tricobalt cluster FcCCo3(CO)9 (1) (where Fc = ferrocenyl) and the redox-active diphosphine ligand 2,3-bis(diphenylphosphino)maleic anhydride (bma) affords the new cluster
(3) in refluxing 1,2-dichloroethane or toluene. The cluster FcCCo3(CO)7(bma) (2), a logical precursor to 3, was observed in solution by IR spectroscopy when cluster 1 and bma were refluxed in the low boiling point solvent CH2Cl2; however, putative 2 could not be isolated due to its rapid conversion to the final product 3. Cluster 3 has been fully characterized in solution by IR and NMR (13C and 31P) spectroscopy and in the solid state by X-ray diffraction analysis.
, as the CH2Cl2 solvate, crystallized in the triclinic space group
for 3602 observed reflections with 13σ(I). Cyclic voltammetric investigations of 3 in CH2Cl2 reveal the presence of three reversible redox responses assigned to the 0/1+, 0/1−1, and 1−/2− redox couples. The nature of the HOMO and the two lowest unoccupied molecular orbitals (LUMO and SUMO) in 3 has been determined by carrying out extended Hückel calculations on the model compound
, the results of which are discussed relative to the observed electrochemistry of 3 and related cluster compounds. 相似文献
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18.
Juan J. Aragn Francisco J. Caada Alfonso Fernndez-Mayoralas Rosa Lpez Manuel Martín-Lomas Daniel Villanueva 《Carbohydrate research》1996,290(2)
By enzymatic β-
-galactosylation of
-xylose a mixture of 4-, 3-, and
(1, 4, and 7, respectively) was obtained in 50% isolated yield. Disaccharides 1, 4, and 7 are substrates of intestinal lactase isolated from lamb small intestine with Km values of 250.0, 4.5, and 14.0 mM, respectively. The mixture was used to monitor the normal decline in lactase activity in rats that takes place after weaning. The data obtained by this method correlated with the levels of intestinal lactase activity in the same animals after being sacrificed. 相似文献
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19.
Claudia Chaves Loureiro Marta Drummond Adriana Magalhães Elisabete SantaClara Miguel Gonçalves João Carlos Winck 《Journal of medical case reports》2009,3(1):1-7
Introduction
There seem to be no published data concerning the clinical impact of populations of hepatitis B virus (HBV) in the hepatic and extrahepatic compartments of HIV-infected people with severe acute hepatitis.Case presentation
A 26-year-old Caucasian man presenting to our hospital with clinical symptoms suggesting acute hepatitis was found to have an acute hepatitis B profile upon admission. He developed fatal fulminant hepatitis and was found to be heavily immunocompromised due to HIV-1 infection. He had a high plasma HBV and HIV load, and analysis of the partial pre-S1/pre-S2 domain showed the presence of mixed infection with D and F genotypes. Analysis of the point mutations within this region revealed the presence of HBV strains with amino acid substitutions at the immunodominant epitopes involved in B or T cell recognition. A homogeneous population of a pre-core mutant strain harbouring the A1896G and A1899G affecting HBeAg expression was invariably found in the liver tissue, plasma and peripheral blood mononuclear cells despite active HBeAg secretion; it was the dominant strain in the liver only, and was characterised by the presence of two point mutations in the direct repeat 1 domain involved in HBV replication activity. Taken together, these mutations are indicative of a highly replicative virus capable of evading immune responses.Conclusion
This case report provides clinical evidence of a possible association between the rapid spread of highly replicative escape mutants and the development of fulminant hepatitis in a heavily immunocompromised patient. Virological surveillance of severe acute hepatitis B may be important in establishing an early treatment strategy involving antiviral drugs capable of preventing liver failure, especially in individuals for whom liver transplantation is not accepted as a standard indication. 相似文献20.
模拟土壤温度升高对湿草甸小叶章生长及生理特性的影响 总被引:3,自引:0,他引:3
采用改进的电缆加热升温方法,研究了土壤温度升高1 ℃~3 ℃对湿草甸小叶章生长及生理特性的影响.结果表明:土壤温度升高明显促进了小叶章植株的生长淹水处理条件下各指标的增长程度依次为:根系总吸收面积(60%)>叶面积(37.32%)>分蘖数(25.81%)>根系体积(24.67%)>株高(18.2%);非淹水处理条件下为:根系总吸收面积(37.47%)>根系体积(36.26%)>叶面积(32.9%)>分蘖数(32.77%)>株高(12.09%).淹水处理条件下小叶章各生理指标对温度升高的响应较为显著,升温淹水处理下小叶章的总叶绿素含量、根部可溶性蛋白含量均呈增加趋势(9.8%和23.88%),而叶片可溶性蛋白和可溶性糖及根部可溶性糖含量显著下降(24.67%、22.92%和35.23%).说明土温升高1 ℃~3 ℃能够显著促进湿草甸小叶章的生长,且其生理特性对土温升高的响应程度因水分条件而异. 相似文献