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Jin Hyoung Kim Young Suk Yu Kyu-Won Kim Jeong Hun Kim 《Histochemistry and cell biology》2010,134(3):277-284
This study was to investigate the effect of the absence of ganglion cells on the development of human retinal vasculature.
Anencephaly (AnC) and age-matched control eyes derived from each three spontaneously aborted fetus (ranging from 15 to 20 weeks
gestation) were subjected to immunofluorescence staining for HIF-1α, Thy-1, glial fibrillary acidic protein (GFAP) and platelet/endothelial cell adhesion molecule (PECAM) and apoptosis assay. In developing
mouse retina, Western blotting for hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) was
performed. Under hypoxic condition (O2 < 1%), cellular proliferation and VEGF mRNA expression in astrocytes were measured. Apoptotic cells in AnC retina were primarily
localized in the ganglion cell layer (GCL), whereas apoptotic cells in normal retina were distributed in the retinoblastic
layer. With increase of apoptotic cells in GCL of AnC retina, HIF-1α expression were severely distinguished in avascular retina
and GFAP expression in junctional area between avascular and vascular retina was much reduced, accompanied by decrease of
PECAM expression compared to normal retina. In developing mouse retina, HIF-1α and VEGF expression were high in hypoxic retina
of early stage with incomplete vascular development and then progressively decreased with regression to arborous pattern of
matured vascular networks. In hypoxic condition, a significant increase in cellular proliferation and VEGF mRNA expression
was observed in astrocytes. Therefore, our results suggest that vascular attenuation in AnC retina could be closely related
to the absence of ganglion cells as the metabolic demander to induce retinal vascular development. 相似文献
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Alagappan VK McKay S Widyastuti A Garrelds IM Bogers AJ Hoogsteden HC Hirst SJ Sharma HS 《Cell biochemistry and biophysics》2005,43(1):119-129
Airflow obstruction in chronic airway disease is associated with airway and pulmonary vascular remodeling, of which the molecular
mechanisms are poorly understood. Paracrine actions of angiogenic factors released by resident or infiltrating inflammatory
cells following activation by proinflammatory cytokines in diseased airways could play a major role in the airway vascular
remodeling process. Here, the proinflammatory cytokines interleukin (IL)-1β, and tumor necrosis factor (TNF)-α were investigated
on cell cultures of human airway smooth muscle (ASM) for their effects on mRNA induction and protein release of the angiogenic
peptide, vascular endothelial growth factor (VEGF). IL-1β (0.5 ng/mL) and TNF-α (10ng/mL) each increased VEGF mRNA (3.9 and
1.7 kb) expression in human ASM cells, reaching maximal levels between 16 and 24 and 4 and 8h, respectively. Both cytokines
also induced a time-dependent release of VEGF, which was not associated with increased ASM growth. Preincubation of cells
with 1μM dexamethasone abolished enhanced release of VEGF by TNF-α. The data suggest that human ASM cells express and secrete
VEGF in response to proinflammatory cytokines and may participate in paracrine inflammatory mechanisms of vascular remodeling
in chronic airway disease. 相似文献
4.
Choi IY Kim SJ Jeong HJ Park SH Song YS Lee JH Kang TH Park JH Hwang GS Lee EJ Hong SH Kim HM Um JY 《Molecular and cellular biochemistry》2007,305(1-2):153-161
The citrus unshiu peel has been used traditionally as a medicine to improve bronchial and asthmatic conditions or cardiac
and blood circulation in Korea, China, and Japan. Here, we report the effects of citrus unshiu peel water extract (CPWE) on
the phorbol myristate acetate (PMA) + calcium ionophore A23187-induced hypoxia-inducible factor-1α (HIF-1α) activation and
inflammatory cytokine production from the human mast cell line, HMC-1 cells. We compared CPWE with hesperidin, a common constituent
of citrus unshiu. CPWE and hesperidin inhibited the PMA + A23187-induced HIF-1α expression and the subsequent production of
vascular endothelial growth factor (VEGF). In addition, CPWE suppressed PMA + A23187-induced phosphorylation of the extracellular
signal-regulated kinase (ERK). We also show that the increased cytokines interleukin (IL)-1β, IL-8, and tumor necrosis factor
(TNF)-α level was significantly inhibited by treatment of CPWE or hesperidin. In the present study, we report that CPWE and
hesperidin are inhibitors of HIF-1α and cytokines on the mast cell-mediated inflammatory responses. 相似文献
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Comparative analysis of vascular endothelial cell activation by TNF-α and LPS in humans and baboons 总被引:7,自引:0,他引:7
As an Old World nonhuman primate, baboons have been extensively used for research on dyslipidemia and atherogenesis. With
increasing knowledge about the endothelium's role in the initiation and progression of atherosclerosis, the value of the baboon
model can be increased by developing it for research on the role of dysfunctional endothelium in atherogenesis. Toward that
goal, we have established and validated methods of isolating and culturing baboon femoral artery endothelial cells (BFAECs)
and compared baboon endothelial cellular characteristics with those of humans. Our results indicated that baboon and human
endothelial cells share similar growth and culture behaviors. As was the case for human endothelial cells, BFAECs responded
to tumor necrosis factor (TNF)-α stimulation with increased expression of adhesion molecules (maximum increase for intracellular
adhesion molecule (ICAM): 1.76±0.26-fold; vascular cell adhesion molecule (VCAM): 1.65±0.25-fold; E-selectin: 2.86±0.57-fold).
However, BFAECs were hyporesponsive to lipopolysaccharide (LPS) (range, 0.25–20 μg/mL) in adhesion molecule expression, whereas
1 μg/mL LPS induced 2.14- to 3.71-fold increases in human endothelial cells. The differential responses to LPS were not related
to TLR-2 and toll-like receptor (TLR)-4 expression on the cell surface. And baboon microvascular endothelial cells had similar
features as BFAECs. We observed constitutive expression of interleukin (IL)-6, IL-8, granulocyte macrophage colony-stimulating
factor (GM-CSF), and monocyte chemoattractant protein (MCP)-1 in both human and baboon endothelial cells, and these cytokines
were further induced by TNF-α and LPS. We also demonstrated that the responses to TNF-α or LPS varied among baboons maintained
under the same dietary and environmental conditions, suggesting that response may be controlled by genetic factors. 相似文献
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Birgit M. Kräling Joyce Bischoff 《In vitro cellular & developmental biology. Animal》1998,34(4):308-315
Summary Human dermal microvascular endothelial cells are used to analyze the functions of microvascular endothelium in vitro. However, the low yield and short lifespan of these cells in culture has limited the types of analysis that could be performed.
Human microvascular endothelial cells are typically grown in media containing supplements such as dibutyryl cyclic AMP, hydrocortisone,
bovine brain extract, and antifungal agents, each of which increase the complexity of experimental design and interpretation
of results. In the present study, endothelial cells were transferred after Ulex europeus-I selection into a simplified medium consisting of Endothelial Basal Medium 131, 10% fetal bovine serum, and 2 ng/ml basic
fibroblast growth factor and analyzed over 3 mo. The human microvascular endothelial cells expressed the endothelial markers
von Willebrand factor, CD31, P-selectin, and E-selectin. In addition, the cells showed increased proliferation in the presence
of basic fibroblast growth factor (0.5 ng/ml) or vascular endothelial cell growth factor (10 ng/ml). Tumor necrosis factor-α-induced
expression of E-selectin was similar in cells at Passages 3, 6, and 12, indicating that the cells maintained responsiveness
to cytokines over several weeks. Furthermore, the endothelial cells attained a typical cobblestone morphology with increased
cellular density and also formed capillarylike tubes on Matrigel. In summary, the human dermal microvascular endothelial cells
display the expected endothelial characteristics when grown for several passages and, therefore, provide a valuable in vitro model for human microvascular endothelium. 相似文献
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Singh AK Patel J Litbarg NO Gudehithlu KP Sethupathi P Arruda JA Dunea G 《Cell and tissue research》2008,332(1):81-88
When rat omentum becomes activated by intraperitoneal injection of inert polydextran particles, these particles are rapidly
surrounded by cells that express markers of adult stem cells (SDF–1α, CXCR4, WT–1) and of embryonic pluripotent cells (Oct–4,
Nanog, SSEA–1). We have cultured such cells, because they may offer a convenient source of adult stem cells, and have found
that they retain stem cell markers and produce high levels of vascular endothelial growth factor for up to ten passages. After
systemic or local injection of these cultured cells into rats with acute injury of various organs, the cells specifically
engraft at the injured sites. Thus, our experiments show that omental stromal cells can be cultured from activated omentum,
and that these cells exhibit stem cell properties enabling them to be used for repair and possibly for the regeneration of
damaged tissues. 相似文献
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Developing antiangiogenic agents using natural products has remained a significant hope in the mainstream of anticancer research.
In the present investigation series of flavonoids possessing di-, tri-, tetra-, and penta-hydroxy substitutions were evaluated
as antiangiogenic agents using in vivo choriallantoic membrane model. The MTT-based cytotoxicity against selected cancer cell
lines was carried out to determine the anticancer potential. The kinetics of free radical scavenging activities of these compounds
was demonstrated using 2,2-diphenyl-1-picryl hydrazine (DPPH) and superoxide anion radicals (SORs). To understand the possible
antiangiogenic mechanism, the selected flavonoids were docked in silico onto the proangiogenic peptides such as vascular endothelial
growth factor (VEGF), hypoxia inducible factor (HIF-1α), and vascular endothelial growth factor receptor-2 (VEGFR2) from human
origin. The results of the study shows that amongst the tested flavonoids, genistein (87.1%), kaempferol, (86.3%), and quercetin
(84.7%) were found to be effective inhibitors of angiogenesis in CAM model. The antiangiogenic, cytotoxic, and antioxidant
activities are discussed in light of structure–activity relationship using in silico approach and other drug-related properties
were also calculated using BioMed CAChe V. 6.1.10. The results of the present study focus the isoflavone genistein, kaempferol,
and quercetin as lead molecules for designing novel anti-tumor/antioxidant agents targeting angiogenesis. 相似文献
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Shunsei Hirohata Yasushi Miura Tetsuya Tomita Hideki Yoshikawa Takahiro Ochi Nicholas Chiorazzi 《Arthritis research & therapy》2006,8(2):R54-10
Bone marrow CD34+ cells from rheumatoid arthritis (RA) patients have abnormal capacities to respond to tumor necrosis factor
(TNF)-α and to differentiate into fibroblast-like cells producing matrix metalloproteinase (MMP)-1. We explored the expression
of mRNA for nuclear factor (NF)κB in RA bone marrow CD34+ cells to delineate the mechanism for their abnormal responses to
TNF-α. CD34+ cells were purified from bone marrow samples obtained from 49 RA patients and 31 osteoarthritis (OA) patients
during joint operations via aspiration from the iliac crest. The mRNAs for NFκB1 (p50), NFκB2 (p52) and RelA (p65) were examined
by quantitative RT-PCR. The expression of NFκB1 mRNA in bone marrow CD34+ cells was significantly higher in RA than in OA,
whereas there was no significant difference in the expression of mRNA for NFκB2 and RelA. The expression of NFκB1 mRNA was
not correlated with serum C-reactive protein or with the treatment with methotrexate or oral steroid. Silencing of NFκB1 by
small interfering RNA abrogated the capacity of RA bone marrow CD34+ cells to differentiate into fibroblast-like cells and
to produce MMP-1 and vascular endothelial growth factor upon stimulation with stem cell factor, granulocyte-macrophage colony
stimulating factor and TNF-α without influencing their viability and capacity to produce β2-microglobulin. These results indicate
that the enhanced expression of NFκB1 mRNA in bone marrow CD34+ cells plays a pivotal role in their abnormal responses to
TNF-α and, thus, in the pathogenesis of RA. 相似文献
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Massimo Mariotti Sara Castiglioni Daniela Bernardini Jeanette AM Maier 《Immunity & ageing : I & A》2006,3(1):4
Background
The functional changes associated with endothelial senescence may be involved in human aging and age-related vascular disorders. Since the inflammatory cytokine interleukin (IL-)1 inhibits endothelial growth, we evaluated the expression of IL-1α, IL-1β and their antagonist, the IL-1 receptor antagonist (IL-1ra), in endothelial in vitro senescence and quiescence. We also examined the expression of IL-1α in human senescent and progeric fibroblasts. 相似文献14.
Masanori Ito Kazuhiro Yoshida Eikai Kyo Ayse Ayhan Hirofumi Nakayama Wataru Yasui Hisao Ito Eiichi Tahara 《Virchows Archiv. B, Cell pathology including molecular pathology》1990,59(1):173-178
We have examined the expression of mRNAs for epidermal growth factor (EGF), transforming growth factor-alpha (TGF-α), EGF
receptor (EGFR), PDGF-A chain (PDGFA), PDGF-B chain (PDGFB) and PDGF receptor (PDGFR) genes in seven human colorectal carcinoma
cell lines and 18 human colorectal carcinomas.
In surgically resected specimens of the 18 colorectal tumors, TGF-α, EGFR, PDGFA, PDGFB and PDGFR mRNAs were detected at various
levels in 15 (83%), 9 (50%), 18 (100%), 8 (44%) and 12 (67%), respectively. They were also detected in normal tissues. Interestingly,
EGF mRNA was detected in only five (28%) of the tumors, but not in normal mucosa. Expression of EGF was also confirmed immunohistochemically
in tumor cells. Of the five tumors expressing EGF, four expressed EGFR mRNA and showed a tendency to invade veins and lymphatics.
All the colorectal carcinoma cell lines expressed TGF-α mRNA, and five cell lines expressed EGFR mRNA simultaneously. Production
of TGF-α protein by DLD-1 and CoLo320DM cells was confirmed by TGF-α specific monoclonal antibody binding assay. The spontaneous3H-thymidine uptake by DLD-1 was suppressed by an anti-TGF-α monoclonal antibody. PDGFA and PDGFB mRNA were also expressed
in four cell lines, but PDGFR and EGF mRNA was not detected. These results suggest that human colorectal carcinomas express
multi-loops of growth factors and that TGF-α produced by tumor cells functions as an autocrine growth factor in human colonic
carcinoma. 相似文献
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Xingqun Ma Yanwen Yao Dongmei Yuan Hongbing Liu Shouju Wang Changsheng Zhou Yong Song 《PloS one》2012,7(12)
Background
Malignant pleural effusion (MPE) is a common complication of lung cancer. One widely used treatment for MPE is Endostar, a recombined humanized endostatin based treatment. However, the mechanism of this treatment is still unclear. The aim of this study was to investigate the effects of Endostar in mice with MPE.Methods and Materials
Lewis lung carcinoma (LLC) cell line expressing enhanced green fluorescent protein (EGFP) was injected into pleural cavity to establish MPE mice model. Mice were randomly divided into four groups. High dose of Endostar (30 mg/kg), low dose of Endostar (8 mg/kg), normal saline, or Bevacizumab (5 mg/kg) was respectively injected into pleural cavity three times with 3-day interval in each group. Transverse computed tomography (CT) was performed to observe pleural fluid formation 14 days after LLC cells injection. Mice were anesthetized and sacrificed 3 days after final administration. The volume of pleural effusion n was measured using 1 ml syringe. Micro blood vessel density (MVD), Lymphatic micro vessel density (LMVD), the expression level of vascular endothelial growth factor A (VEGF-A) and VEGF-C were observed by immunohistochemistry (IHC) staining.Results
The volume of pleural effusion as well as the number of pleural tumor foci, MVD and the expression of VEGF-A were significantly reduced in high dose of Endostar treat group. More importantly, LMVD and the expression of VEGF-C were markedly lower in treat group than those in the other three control groups.Conclusion
Our work demonstrated that Endostar played an efficient anti-cancer role in MPE through its suppressive effect on angiogenesis and lymphangiogenesis, which provided a certain theoretical basis for the effectiveness of Endostar on the MPE treatment. 相似文献18.
Hongwei Si Dongmin Liu 《Apoptosis : an international journal on programmed cell death》2009,14(1):66-76
Isoflavone genistein may have beneficial effects on vascular function, but the mechanism is unclear. Here, we investigated
whether genistein protects vascular endothelial cells against apoptosis induced by tumor necrosis factor-α. We show that genistein
significantly inhibited TNF-α-induced apoptosis in human aortic endothelial cells as determined by caspase-3 activation, 7-amino
actinomycin D staining, in situ apoptotic cell detection and DNA laddering. The anti-apoptotic effect of genistein was associated
with an enhanced expression of Bcl-2 protein and its promoter activity. Inhibition of extracellular signal-regulated kinase
1/2, protein kinase A, or estrogen receptors had no effect on the cytoprotective effect of genistein. However, inhibition
of p38 mitogen-activated protein kinase (p38) completely abolished this genistein effect. Accordingly, stimulation of HAECs
with genistein resulted in rapid activation of p38β, but not p38α. These findings provide the evidence that genistein acts
as a survival factor for vascular ECs to protect cells against apoptosis via activation of p38β. Preservation of the functional
integrity of the endothelial monolayer may represent an important mechanism by which genistein exerts its vasculoprotective
effect. 相似文献
19.
Jonathan Eckard Jisen Dai Jing Wu Jinlong Jian Qing Yang Haobin Chen Max Costa Krystyna Frenkel Xi Huang 《Cancer cell international》2010,10(1):28
Background
Young women diagnosed with breast cancer are known to have a higher mortality rate from the disease than older patients. Specific risk factors leading to this poorer outcome have not been identified. In the present study, we hypothesized that iron deficiency, a common ailment in young women, contributes to the poor outcome by promoting the hypoxia inducible factor-1α (HIF-1α and vascular endothelial growth factor (VEGF) formation. This hypothesis was tested in an in vitro cell culture model system. 相似文献20.
Ester Fonsatti Elda Lamaj Sandra Coral Luca Sigalotti Gianpaolo Nardi Aldo Gasparollo Mario P. Colombo Maresa Altomonte Michele Maio 《Cancer immunology, immunotherapy : CII》1999,48(2-3):132-138
Melanoma cells constitutively release intercellular adhesion molecule 1 (ICAM-1) as soluble ICAM-1 (sICAM-1), and its levels
are elevated in melanoma patients and correlate with disease progression. However, this correlation is not absolute, suggesting
that specific characteristics of neoplastic cells and/or ICAM-1-positive non-neoplastic cells may influence the amounts of
circulating sICAM-1. In this study, we found a weak correlation (r = 0.55; r
2 = 0.3) between sICAM-1 release by 40 metastatic melanomas (36 primary cultures and 4 cell lines), and ICAM-1 expression on
neoplastic cells. In addition, melanoma-secreted interleukin-1α (IL-1α) (1/40) but not vascular endothelial growth factor
(VEGF) (29/40), significantly (P < 0.05) up-regulated the shedding of sICAM-1 by human umbilical vein endothelial cells (HUVEC). This was completely abolished
by IL-1α/β neutralizing antibodies both at the protein and mRNA level. Altogether, our results suggest that (i) the extent
of sICAM-1 release is distinctive for individual melanomas and can be independent of ICAM-1 expression; (ii) tumor endothelia
may sustain levels of sICAM-1 in selected melanomas; (iii) melanoma-released VEGF does not affect ICAM-1 expression and sICAM-1
release by HUVEC. Melanoma-derived sICAM-1 inhibits cell-mediated cytotoxicity of melanoma cells; therefore, constitutive
levels of sICAM-1 release and IL-1α secretion by individual melanomas can differentially influence tumor progression and the
clinical effectiveness of cytotoxic-cell-based vaccines.
Received: 15 October 1998 / Accepted: 17 February 1999 相似文献