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5.
An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110T, was isolated from the surface-sterilised root of Coffea arabica L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110T was a member of the genus Streptomyces and showed the closest similarities to Streptomyces buecherae AC541T (99.2%), followed by Streptomyces rapamycinicus NRRL B-5491T (99.1%), Streptomyces luteoverticillatus NBRC 3840T (99.1%), Streptomyces coerulescens NBRC 12758T (99.1%), and Streptomyces iranensis HM 35T (99.0%). Strain CA3R110T contained LL-diaminopimelic acid in cell peptidoglycan, MK-9(H6), and MK-9(H8) as major menaquinone, iso-C16:0, iso-C15:0, C16:0 as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus Streptomyces. A low digital DNA–DNA hybridization (<?55.7%) and average nucleotide identity-blast (ANIb) (<?92.2%) values revealed that strain CA3R110T could be distinguished from any known Streptomyces species. With the differences in phenotypic and genotypic data, strain CA3R110T represents a novel species of genus Streptomyces, for which the name Streptomyces endocoffeicus sp. nov. is proposed. The type strain is CA3R110T (=?TBRC 11245T?=?NBRC 114296T). 相似文献
7.
Two novel actinobacterial strains, MS1-9 T and NGC1-4, were isolated from roots of Musa (ABB) cv. ‘Kluai Namwa’, collected from Chachoengsao province, and Musa (ABB) cv. ‘Kluai Chang’, from Suphan Buri province, Thailand, respectively. Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B ( gyrB) gene and whole-genome sequences emphasised that the strains MS1-9 T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363 T, M. krabiensis JCM 12869 T and M. avicenniae DSM 45758 T, respectively. Strains MS1-9 T and NGC1-4 contained meso-diaminopimelic acid in cell-wall peptidoglycan. Whole-cell sugars were glucose, xylose, mannose, and ribose. The acyl type of peptidoglycan was glycolyl. MK-10(H 6), MK-9(H 6), and MK-10(H 8) were presented as the major menaquinones. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol were detected as predominant phospholipid profiles. The major cellular fatty acids consisted of iso-C 15:0, anteiso-C 15:0, anteiso-C 17:0, iso-C 17:0 and C 17:0. The DNA G + C content of strains MS1-9 T and NGC1-4 were 72.2 and 72.3 mol%, respectively. Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9 T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp. nov. is proposed. The type strain is MS1-9 T (=JCM 32149 T = TISTR 2659 T). 相似文献
8.
The taxonomic positions of soil isolates known as Streptomyces groups A, B and C were clarified. Comparative 16S rDNA sequence studies indicated that representatives of all three taxa
formed distinct phyletic lines within the Streptomyces tree though the group A strains were shown to be related to Streptomyces griseus and associated validly described species. The taxonomic integrity of all three groups was highlighted by DNA:DNA relatedness
and ribotype data though the group A strains encompassed a higher degree of genetic variation than the group B and C strains.
In light of these and earlier phenotypic data it is proposed that Streptomyces groups A, B and C be given species status as Streptomyces sanglieri sp. nov., Streptomyces aureus sp. nov. and Streptomyces laceyi sp. nov., respectively.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
11.
A novel actinomycete strain designated CN-207 T was isolated from northern Tunisian soil. This strain exhibited potent broad spectrum antibacterial activity against clinical isolates of methicillin-resistant Staphylococcus species and several other Gram-positive and Gram-negative bacteria. Strain CN-207 T developed greyish aerial mycelium and pale grey substrate mycelium on yeast extract/malt agar. The isolate produced branching vegetative mycelia with sporangiophores bearing sporangia developing at a late stage of growth. The sporangia contained smooth, non-motile spores. Chemotaxonomic characteristics of strain CN-207 T were typical of the Streptomyces genus. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CN-207 T belonged to the genus Streptomyces, and was most closely related to Streptomyces griseoincarnatus DSM 40274 T, Streptomyces variabilis DSM 40179 T, Streptomyces labedae DSM 41446 T and Streptomyces erythrogriseus DSM 40116 T. Low DNA–DNA relatedness values were recorded between strain CN-207 T and its closest phylogenetic neighbours. Strain CN-207 T was also distinguished from the nearest phylogenetic neighbours using a combination of morphological and phenotypic characteristics. On the basis of its phenotypic and molecular properties, strain CN-207 T is considered as a novel species of the Streptomyces genus, for which the name Streptomyces tunisiensis sp. nov. is proposed. The type strain is CN-207 T (=JCM 17589 T = DSM 42037 T). 相似文献
12.
Two novel anthraquinones, lupinacidins A (1) and B (2), have been isolated from the culture broth of a new endophytic actinomycete belonging to the genus Micromonospora. Lupinacidins were found to show significant inhibitory effects on the invasion of murine colon 26-L5 carcinoma cells without inhibiting cell growth. 相似文献
13.
A polyphasic analysis was carried out to clarify the taxonomic status of four marine actinomycete strains that share a phylogenetic relationship and phenotypic characteristics with the genus Salinispora. These strains formed a distinct lineage within the Salinispora 16S rRNA and gyrB trees and were found to possess a range of phenotypic properties and DNA:DNA hybridization values that distinguished them from the type strains of the two validly named species in this genus, Salinispora tropica (CNB-440 T, ATCC BAA-916 T) and Salinispora arenicola (CNH-643 T, ATCC BAA-917 T). The combined genotypic and phenotypic data support this conclusion. It is proposed that the strains be designated as Salinispora pacifica sp. nov., the type strain of which is CNR-114 T (DSMZ YYYYT = KACC 17160 T). 相似文献
14.
An alkaliphilic actinobacterium, designated EGI 80050 T, was isolated from a desert soil sample of Xinjiang, north-west China, and characterized by a polyphasic approach. The isolate was observed to produce purple orange-yellow aerial mycelium and dark orange-yellow substrate mycelium on yeast extract-malt extract agar medium. Whole-cell hydrolysates of strain EGI 80050 T were found to contain ll-diaminopimelic acid as the diagnostic diamino acid, and galactose, glucose, rhamnose and mannose as the main sugars. The major fatty acids identified were C 16:0-iso (36.8 %), C 15:0-anteiso (17.3 %), 15:0-iso (13.2 %) and 14:0-iso (10.5 %). The predominant menaquinones detected were MK-9(H 6) and MK-9(H 8), while the characteristic polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, phosphatidylmethylethanolamine and three unknown phospholipids. The G+C content of the genomic DNA was determined to be 67.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences affiliated the strain EGI 80050 T to the genus Streptomyces. Levels of 16 rRNA gene sequence similarities between strain EGI 80050 T and Streptomyces candidus NRRL ISP-5141 T, Streptomyces cremeus NBRC 12760 T, Streptomyces spiroverticillatus NBRC 12821 T, Streptomyces violaceorectus NBRC 13102 T, Streptomyces cinereoruber subsp. cinereoruber NBRC 12756 T were 96.7, 96.6, 96.6, 96.6 and 96.6 %, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic data, strain EGI 80050 T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces fukangensis sp. nov. (type strain EGI 80050 T = BCRC 16945 T = JCM 19127 T) is proposed. 相似文献
15.
A strain of Streptomyces, MBRL 179 T, isolated from a sample from a Limestone quarry located at Hundung, Manipur, India, was characterized by polyphasic taxonomy. The strain formed a monophyletic clade with Streptomyces spinoverrucosus NBRC 14228 T (16S rRNA gene sequence similarity of 99.3 %) in the Neighbour-joining tree. DNA–DNA hybridization experiment gave a DNA–DNA relatedness value of 34.7 % between MBRL 179 T and S. spinoverrucosus NBRC 14228 T. Strain MBRL 179 T contained LL-diaminopimelic acid, xylose, glucose, and mannose in the whole cell-wall hydrolysates along with small amount of ribose. The major polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside, with other unknown phospholipids and aminophospholipid. MK-9(H 6), MK-9(H 8) and MK-9(H 4) were the predominant menaquinones detected. The major fatty acids were anteiso-C 16:0 (28.1 %), iso-C 16:0 (20.3 %), C 16:0 (9.4 %) and anteiso-C 17:0 (8.3 %). The G+C content of the genomic DNA was 71.1 %. Based on the polyphasic experiment results, the strain MBRL 179 T merits recognition as a representative of a novel species of the genus Streptomyces for which the name Streptomyces muensis sp. nov. is proposed; the type strain is MBRL 179 T (=JCM 17576 T = KCTC 29124 T). 相似文献
18.
During the investigation of exploring potential sources of novel species and natural bioactives, a novel actinomycete, designated strain HIT-DPA4T, was isolated from a soil sample, which was collected from Nanjing, Jiangsu Province, PR China and characterized using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities and the result of phylogenetic analysis, strain HIT-DPA4T was most closely related to Streptomyces cyaneus CGMCC 4.1671 T, and shared the highest sequence similarity of 98.76%. In addition, the cell walls of the species HIT-DPA4T contained LL-diaminopimelic acid as the diagnostic diamino acid and the whole-cell hydrolysates were identified as glucose and ribose, and the principal phospholipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and phosphatidylmonomethylethanolamine. MK-9(H6) and MK-9(H4) were predominant menaquinones; and C16:0, anteiso-C15:0 and C15:0 as major cellular fatty acids of the organism HIT-DPA4T. Gene Ontology database analysis and antiSMASH server predicted results displayed that strain HIT-DPA4T was a promising classification units, which has various types of functions and contains multiple biosynthetic gene clusters with the similarity more than 80%. Multilocus sequence analysis (MLSA) of five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) illustrated that Streptomyces luteolifulvus formed a separate branch in the genus Streptomyces. However, a combination of low level of DNA-DNA relatedness and physiological properties indicated that strain HIT-DPA4T can be distinguished from its phylogenetically related species Streptomyces cyaneus CGMCC 4.1671 T. Moreover, gene synteny research could be further differed organism HIT-DPA4T from similarity species. Therefore, the strain is concluded to represent a novel species of the genus Streptomyces, for which the name Streptomyces luteolifulvus sp. nov. is proposed. The type strain is HIT-DPA4T (=?CGMCC 4.7558 T?=?TISTR 2751 T).
相似文献
19.
A novel actinomycete, designated strain KLBMP 4601 T, was isolated from the root of the medicinal plant Curcuma phaeocaulis collected from Sichuan Province, south-west China. The strain produced extensively branched substrate and aerial hyphae that carried straight to flexuous spore chains. Chemotaxonomic properties of this strain were consistent with those of members of the genus Streptomyces. The cell wall of strain KLBMP 4601 T contained ll-diaminopimelic acid as the characteristic diamino acid. The major menaquinone was MK-9(H 4), with minor amounts of MK-9(H 6), MK-9(H 8) and MK-10(H 2). The major fatty acids were C 16:0, iso-C 16:0, C 18:1ω9 c and C 16:1, iso G. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KLBMP 4601 T belongs to the genus Streptomyces and is most closely related to Streptomyces armeniacus JCM 3070 T (97.9 %), Streptomyces pharmamarensis PM267 T (97.6 %) and Streptomyces artemisiae YIM 63135 T (97.5 %). The 16S rRNA gene sequence similarity between strain KLBMP 4601 T and other members of this genus were lower than 97.5 %. DNA–DNA hybridization studies of strain KLBMP 4601 T with the three closest species showed relatedness values of 36.3 ± 4.2 %, 27.3 ± 0.6 %, and 30.9 ± 2.5 %, respectively. On the basis of chemotaxonomic, phenotypic and genotypic characteristics, it is evident that strain KLBMP 4601 T represents a novel species of the genus Streptomyces, for which the name Streptomyces phytohabitans sp. nov. is proposed. The type strain is KLBMP 4601 T (=KCTC 19892 T = NBRC 108772 T). 相似文献
20.
Four quinazolinones, new naturally occurring 1 and three known derivatives 2– 4, along with previously synthesized 4-phenylbut-3-enamide ( 5) and three known compounds, 2-acetamido benzamide, 1 H-indole-3-carbaldehyde, and 2-phenylacetamide, were isolated from an actinomycete, Streptomyces sp. BCC 21795. The structures were elucidated based on the analysis of NMR spectroscopic and mass spectrometric data. Compound 1 exhibited strong cytotoxic activity to Vero cells with IC 50 3.30 μg/mL. 相似文献
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