刺桐姬小蜂对不同品种刺桐属植物为害差异性研究

通过对广东境内刺桐属植物与刺桐姬小蜂分布情况的调查研究,发现广东省主要有刺桐、金脉刺桐、鸡冠刺桐、象牙花和龙牙花等5种刺桐属植物分布,广东省13个地巾级行政区有刺桐姬小蜂发生;5种主要奇主中,刺桐、金脉刺桐易感虫,而鸡冠刺桐、象牙花、龙牙花较抗虫.     

金脉刺桐嫩枝上刺桐姬小蜂虫瘿分布规律研究

刺桐姬小蜂Quadrastichus erythrinae Kim是重要外来入侵害虫,危害刺桐属多种植物。本文调查研究了金脉刺桐嫩枝上刺桐姬小蜂虫瘿的数量分布和空间格局。结果显示,金脉刺桐嫩枝上靠近生长点（芽头）一端刺桐姬小蜂虫瘿数量较多,15 cm以内占90%以上,距离越远越少,其虫瘿数量比率（Y）和枝条长度（X）关系方程为Y= 72577e-0138X。对频次分布的分析结果显示,刺桐嫩枝上虫瘿主要以虫瘿集体形式出现。获得的虫瘿空间格局主要参数平均拥挤度M*、丛生指数I、聚块性指数M*/M等均显著大于1,表明虫瘿是聚集分布且聚集程度较强。建立的Iwao M*-M方程为M*=175+351M、Taylor幂函数法则模型为lg s2=0554+1828lg〖AKx-〗。     

刺桐姬小蜂发生习性及其虫瘿形成分析

刺桐姬小蜂Quadrastichus erythrinaeKim是新发现的重要入侵害虫,在广东省深圳地区普遍发生且危害严重,受害植株超过10000株。寄主按受害程度依次为:杂色刺桐Erythrina variegata、金脉刺桐E.variegatevar.orientialis、鸡冠刺桐E.cristagalli和龙牙花E.corallodendron。移栽2年内的刺桐树受害严重。根据虫瘿形状,将其分为球形虫瘿、卷叶形虫瘿、肥厚叶虫瘿、分散虫瘿、粗柄形虫瘿、嫩枝肿大虫瘿、花蕾肿大虫瘿并对其形成过程进行分析。首次报道了刺桐姬小蜂为害鸡冠刺桐花蕾。刺桐姬小蜂在深圳无休眠和滞育现象,常年发生。     

中国大陆一新外来入侵种--刺桐姬小蜂

报道了最近发生在广东省深圳市、福建省厦门市等地刺桐等桐属植物上的一种新的外来人侵害虫——刺桐姬小蜂Quadrastichus erythrinae Kim。对其分类地位、形态特征、生物学特性、分布、寄主、为害症状等做了简要介绍,提出防治措施,并对其风险分析进行评估。     

刺桐姬小蜂生物学特性研究

刺桐姬小蜂Quadrastichus erythrinae Kim是新近发现的重要检疫性有害生物,在新加坡、毛里求斯、留尼汪、中国台湾、美国夏威夷和中国大陆深圳对刺桐属植物造成严重危害。本文结合田间观察和室内实验对刺桐姬小蜂生物学特性进行了较为系统的研究。结果表明： 刺桐姬小蜂成虫活动与温度、光照密切相关。补充营养能显著延长雌成虫寿命,但对雄成虫寿命无影响。成虫性比随环境温度变化而变化。该虫的平均怀卵量为275.8粒,30℃时成虫的产卵量和产卵率最高,分别为203.63粒和73.83%。发育起点温度和世代有效积温分别为13.37℃和458.27日·度。温度与发育历期呈显著的负相关（r**=-0.9161）。     

刺桐树11种挥发物对刺桐姬小蜂的野外引诱活性研究

为探明刺桐姬小蜂Quadrastichus erythrinae Kim对寄主植物上11种挥发物（4-溴丁醚苄酯、1,2-二甲苯、3-蒈烯、乙酸叶醇酯、苯甲腈、α－法呢烯、奎诺二甲基丙烯酸酯、柏木烯醇、芳樟醇、水杨酸甲酯、法呢醇）的野外引诱活性，本试验分别通过不同挥发物引诱板及不同颜色引诱板对刺桐姬小蜂进行了野外引诱活性研究，结果表明：苯甲腈的引诱效果最好，除了和1,2-二甲苯差异不显著外，跟其他挥发物的引诱效果差异显著；对雌成虫的引诱作用与1,2-二甲苯、3-蒈烯差异不显著，与其他成分差异显著；对雄成虫的引诱作用苯甲腈和水杨酸甲酯的引诱作用最好，与其他成分均差异显著；在引诱板颜色试验中，刺桐姬小蜂成虫对带荧光的翠绿色具有较强的趋性，该颜色除了和无荧光的黄色差异不显著外，跟其他颜色差异显著。     

新入侵种刺桐姬小蜂在中国的危险性评估

采用GARP生态位模型预测分析了刺桐姬小蜂Quadrastichus erythrinaeKim在中国的潜在地理分布,并参照国际上有害生物危险性定量分析方法,对刺桐姬小蜂在中国的危险性作出综合评价。结果表明:中国东南部大部分地区都是该虫的适生区。在福建、广东、海南地区风险值为2.0585,存在高度危险性;在其他东南部省区存在中度危险性。据此提出了针对性的风险管理措施。     

刺桐姬小蜂的虫瘿

刺桐姬小蜂Quadrastichus erythrinaeKim雌虫在刺桐幼嫩的组织内产卵,随着幼虫的生长,这些部位会增生,产生形式多样的虫瘿,从而影响刺桐的正常生长,特别是枝叶不能展开,影响光合作用。由于刺桐姬小蜂发育速度快,夏季平均不到1个月完成1个世代,目前严重为害海南、广东等地的多种     

基于ITS条形码序列的刺桐姬小蜂分子鉴定

【目的】刺桐姬小蜂Quadrastichus erythrinae Kim体型小,传统的形态学鉴定方法难以快速准确识别。【方法】本研究测定了刺桐姬小蜂的rDNA ITS1和ITS2序列,根据18S rDNA部分序列,利用MEGA的最大相似法(Maximum Likehood)构建系统发育树。根据刺桐姬小蜂ITS1和ITS2序列设计了特异引物,应用特异引物对单只刺桐姬小蜂进行PCR扩增,可稳定地扩增出明显的目的DNA条带。【结果】研究表明,基于ITS基因的DNA条形码技术可以用于刺桐姬小蜂的快速准确鉴定。【结论】因此,采用ITS1和ITS2区的特异性引物可对刺桐姬小蜂进行快速分子鉴定。     

刺桐姬小蜂在五(品、变)种刺桐属植物上的危害特性及程度

针对深圳市梧桐山林场、福田区新洲滨河立交、笔架山公园等不同生境5(品、变)种刺桐属植物(刺桐东方变种、金脉刺桐、本地刺桐、龙牙花刺桐、鸡冠刺桐)上刺桐姬小蜂的危害特点及发生情况进行调查。结果表明:刺桐姬小蜂危害最严重的是刺桐东方变种及金脉刺桐,龙牙花刺桐受轻微的危害,本地种刺桐和鸡冠刺桐完全不受害。此外,在刺桐东方变种不同方位树冠中,树冠东边的叶片3级危害百分比值较大,树冠东边与中部的危害指数差异显著;在刺桐东方变种和金脉刺桐的树冠上、中、下3个层次中,以树冠中上部的叶片整体受害较为严重。     

Using host plant relationships to help determine origins of the invasive Erythrina gall wasp, Quadrastichus erythrinae Kim (Hymenoptera: Eulophidae)

The Erythrina gall wasp has recently invaded a wide swath of Asian and Pacific countries, causing severe damage to several species of Erythrina trees. It poses an imminent threat to native Erythrina species in Latin America, Asia, Australia and the Pacific. While an African origin of the pest is presumed, it’s exact home on the continent remains unknown. We examined host plant relationships of the wasp using 71 of the world’s species of Erythrina that are planted in the botanical gardens of Hawaii. Observational and experimental data indicate that species endemic to Africa are more resistant to the wasp than those from other continents. Complete absence of galling on all Erythrina native to Benin, Burundi, Congo, Gambia, Lesoto, Rwanda, and Somalia make those countries highly unlikely to be the origin of the wasp. Mozambique, South Africa, Swaziland, and Zimbabwe were also shown to be unlikely sources. We present susceptibility indices of all 71 Erythrina species, including a number of economically useful trees, and we provide a warning for several species of conservation concern.     

The advantage of attending ants and gall aggregation for the gall wasp Andricus symbioticus (Hymenoptera: Cynipidae)

Summary Gall clusters of Andricus symbioticus secrete a sweet and sticky food attractive to ants. An ant exclusion experiment demonstrated the selective advantage of attending ants and gall aggregation for A. symbioticus. This gall wasp interacts with the gall-attending ants only through the host plant. Evolution of this symbiotic relationship seems to be associated with gall aggregation.Contribution from Laboratory of Entomology Kyoto Prefectural University, No. 249     

A phylogenetic analysis of higher-level gall wasp relationships (Hymenoptera: Cynipidae)

We present the most comprehensive analysis of higher-level relationships in gall wasps conducted thus far. The analysis was based on detailed study of the skeletal morphology of adults, resulting in 164 phylogenetically informative characters, complemented with a few biological characters. Thirty-seven cynipid species from thirty-one genera, including four genera of the apparently monophyletic Cynipini and almost all of the genera in the other tribes, were examined. The outgroup included exemplar species from three successively more distant cynipoid families: Figitidae (the sister group of the Cynipidae), Liopteridae and Ibaliidae. There was considerable homoplasy in the data, but many groupings in the shortest tree were nonetheless well supported, as indicated by bootstrap proportions and decay indices. Partitioning of the data suggested that the high level of homoplasy is characteristic of the Cynipidae and not the result of the amount of available phylogenetically conservative characters being exhausted. The analysis supported the monophyly of the Cynipini (oak gall wasps) which, together with the Rhoditini (the rose gall wasps), Eschatocerini and Pediaspidini formed a larger monophyletic group of gall inducers restricted to woody representatives of the eudicot subclass Rosidae. The inquilines (Synergini) were indicated to be monophyletic, whereas the Aylacini, primarily herb gall inducers, appeared as a paraphyletic assemblage of basal cynipid groups. The shortest tree suggests that the Cynipidae can be divided into three major lineages: one including the inquilines, the Aylacini genera associated with Rosaceae, and Liposthenes ; one consisting entirely of Aylacini genera, among them Aulacidea , Isocolus and Neaylax ; and one comprising the woody rosid gallers (the oak and rose gall wasps and allies), the Phanacis-Timaspis complex and the Aylacini genera associated with Papaveraceae.     

Population dynamics of the chestnut gall wasp,Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae)

Summary During the course of a 10-year investigation on the population dynamics of Dryocosmus kuriphilus, a rapid decrease in the number of individuals was noticed in the adult stage. To detect the role of predation by spiders in causing this high adult mortality, spiders collected from the survey station were tested for their reaction to Drycosmus antiserum. About 2050% of the spiders collected during the emergence period of D. kuriphilus reacted positively, showing that the species of Thomisidae, Argiopidae, Agelenidae and Salticidae were the important predators. The predation ratio for the total adult wasp population was estimated at 8.1% in 1968, 20.2% in 1969 and much higher in 1970, increasing with decreasing population density of the wasps. However, the predation by spiders could not account for all of the adult mortality of D. kuriphilus.     

Constraints on growth and allocation patterns of Silphium integrifolium (Asteraceae) caused by a cynipid gall wasp

Summary Life-history traits of 101 clones from two populations of Daphnia magna were measured under controlled environmental conditions in the laboratory. Some individuals had four juvenile instars, others had five. This depended on their length at birth and on the population they came from. Females in the group with five juvenile instars were smaller at birth but larger and older at maturity than those with four juvenile instars. Within groups of females with equal numbers of preadult instars (instar groups) age and size at maturity increased with size at birth. This relationship differed significantly among instar groups for both age and size at maturity. Significant differences in age and size at maturity between two populations became non-significant when size at birth was used as a covariable in AN-COVA. Within populations, size at birth depended on the clone and on the parity of the clutch. First-clutch offspring were considerably smaller than those from later clutches. The results suggest that variability in life-history traits is common within and between clones, but that most of this variation can be accounted for by size at birth and the number of pre-adult instars.     

The effect of hosptlant and parasitoids on the reproductive success of the parthenogenetic gall wasp Diplolepis rosae (Hymenoptera,Cynipidae)

Summary The univoltine cynipid gall wasp Diplolepis rosae reproduces by an obligate homozygosity promoting system known as gamete duplication. The wasp is confined to roses (Rosa spp) on which it induces large, complex and multichambered galls. In southern Sweden, D. rosae was found to parasitize Rosa canina, R. dumalis, R. rubiginosa, R. villosa, R. sherardi and R. rubrifolia, but not R. majalis and R. rugosa. The distribution of galls shows that there are differences in the relation between wasp and hosplant with respect both to species and individual plants. There is a positive correlation between wasp size and gall (clutch) size. Parasitoid pressure was found to be high, causing D. rosae an estimated average larval loss of approximately 75%, mainly due to the attack of the ichneumonid wasp Orthopelma mediator. The very common cynipid inquiline Periclistus brandtii does not seem to have any negative effects. Overall parasitism and probability of no hatched offspring per gall decrease with increasing gall (clutch) size. The probability of loosing all of a given number of offspring decreases with the number of galls produced. It is suggested that D. rosae, in order to escape parasitoids, needs high ability to establish new colonies. Hence the production of many comparatively small galls, which increases the chance of leaving any offspring, rather than the production of few large galls, maximizing the number of offspring, should be favoured by selection.     

Detection of an invasive gall-inducing pest, Quadrastichus erythrinae (Hymenoptera: Eulophidae), causing damage to Erythrina variegata L. (Fabaceae) in Okinawa Prefecture, Japan

In 2005, Quadrastichus erythrinae Kim, 2004 (Hymenoptera: Eulophidae), which induces stem, petiole, and leaf galls on Erythrina variegata L. (Fabaceae), was found on the following six islands in Okinawa Prefecture, Japan: Okinawa, Kume, Miyako, Ishigaki, Iriomote, and Hateruma. Galls were also found in Vietnam. In Japan, no further infestation records have been reported from any of Japan's other south‐western prefectures where Erythrina species grow. Because no Erythrina galls were observed in Okinawa Prefecture before 2005, Q. erythrinae seems to have invaded quite recently.