Oxidation of [Ir(η-C5Me5)(C8H4S8)] and crystal structures of [IrI(η-C5Me5)(C8H4S8)](I3) and [IrI(η-C5Me5)(C8H4S8)](I3)1/2(I7)1/2

[Ir(η⁵-C₅Me₅)(C₈H₄S₈)] (1) [ = 2-{(4,5-ethylenedithio)-1,3-dithiole-2-ylidene}-1,3-dithiole-4,5-dithionate(2−)] was reacted with iodine in dichloromethane to afford one-electron- and two-electron-oxidized species [IrI(η⁵-C₅Me₅)(C₈H₄S₈)] (2), [IrI(η⁵-C₅Me₅)(C₈H₄S₈)](I₃) (3) and [IrI(η⁵-C₅Me₅)(C₈H₄S₈)](I₅) (4). The oxidized species exhibit electrical conductivities of (1.1-5.0) × 10⁻⁶ S cm⁻¹ measured for compacted pellets at room temperature. The X-ray crystal structures of the two-electron-oxidized complexes 3 and 4 revealed the Ir-I bonds for both of them and the presence of for 3 and ions for 4 as the counter anions. They have many S-S and S-I non-bonding contacts to form two-dimensional molecular interaction sheets in the solid state.     

The effect of prostaglandins A2,E1,E2,15 methyl E2, 16, 16 dimethyl E2 and F2α on erythropoiesis

Prostaglandins A₂, E₁, E₂, methylated E₂s and F₂α affected erythropoiesis and/or erythropoietin (Ep) production. This action is indicated in the exhypoxic, polycythemic mouse where radioiron incorporations into RBC increased after administration of these compounds. The kidney and liver have been indicated through previous studies, to actively participate in Ep production. By the removal of one of these active sites in a murine system treated with prostaglandins it is shown that a response is reflected in Ep levels. Interference of the action of prostaglandins (PG) is altered by the removal of one of these target sites of Ep production. The erythropoietic responses elicited by PGA₂, E₁, and perhaps the methylated PGE₂s act through the liver whereas PGE₂ may operate through a renal pathway for its response. PGF_2α reveals no effect on erythropoietic activity and is no different than that observed for vehicle-treated controls. The prostaglandins tested appear to act primarily through the kidney or liver but the possibility exists that some yet undetermined organ site may also be involved.     

柞蚕抗菌肽CA1的分离

应用FPLC、HPLC系统配合MALDI TOF MS等技术 ,分离得到一个以灭活的Escherichiacoli诱导产生的具有明显杀菌活性的柞蚕抗菌肽CA₁。自动蛋白质序列分析仪测定其一级结构为WNPFKELERAGSRVRDAIISAGVAVATVAQATAILK ,含有 36个氨基酸残基 ,经联机检索 ,与cecropinD有 88%的同源性 ,仅有 4个氨基酸残基的差异 ,其中铰链区第 2 1～ 2 3位氨基酸为AGV ,与已知柞蚕抗菌肽A、D铰链区AGP有所不同 ,提示抗菌肽存在多态性。     

PKCγ过表达诱导C3H10T1/2细胞生长失控的初探

通过DNA重组构建蛋白激酶C_γ(PKC_γ)亚类的重组质粒并经基因转染技术和DNA印迹、蛋白质印迹与PKC活性分析,获得了过表达PKC_γ的C₃H₁₀T_1/2细胞——NCP4.NCP4细胞生长速率提高,流式细胞光度术检测表明,NCP4细胞G1期百分率下降,S期和G2+M期百分率升高,与对照组细胞相比,血清依赖性明显下降,贴壁依赖性降低,在软琼脂中形成小集落,出现部分转化表型.进一步检测,首次观察到NCP4细胞中癌基因c-sis表达明显增强,这可能是NCP4细胞血清依赖性下降的分子机理之一.实验表明,在正常C₃H₁₀T_1/2细胞中PKC_γ的过表达可直接导致细胞增殖加速并可诱导出现部分转化特征.     

Critical evaluation of electron transfer kinetics in P700–FA/FB, P700–FX, and P700–A1 Photosystem I core complexes in liquid and in trehalose glass

This work aims to fully elucidate the effects of a trehalose glassy matrix on electron transfer reactions in cyanobacterial Photosystem I (PS I). Forward and backward electron transfer rates from A_1A^? and A_1B^? to F_X, and charge recombination rates from A₀^?, A_1B^?, A_1A^?, F_X^?, and [F_A/F_B]^? to P₇₀₀⁺ were measured in P₇₀₀–F_A/F_B complexes, P₇₀₀–F_X cores, and P₇₀₀–A₁ cores, both in liquid and in a trehalose glassy matrix at 11% humidity. By comparing CONTIN-resolved kinetic events over 6 orders of time in increasingly simplified versions of PS I at 480?nm, a wavelength that reports primarily A_1A^?/A_1B^? oxidation, and over 9 orders of time at 830?nm, a wavelength that reports P₇₀₀⁺ reduction and A₀^? oxidation, assignments could be made for nearly all of the resolved kinetic phases. Trehalose-embedded PS I samples demonstrated partially arrested forward electron transfer. The fractions of complexes in which electron transfer did not proceed beyond A₀, A₁ and F_X were 53%, 16% and 22%, respectively, with only 10% of electrons reaching the terminal F_A/F_B clusters. The ~10?μs and ~150?μs components in both liquid and trehalose-embedded PS I were assigned to recombination between A_1B^? and P₇₀₀⁺ and between A_1A^? and P₇₀₀⁺, respectively. The kinetics and amplitudes of these resolved kinetic phases in liquid and trehalose-embedded PS I samples could be well-fitted by a kinetic model that allowed us to calculate the asymmetrical contribution of the A_1A^? and A_1B^? quinones to the electrochromic signal at 480?nm. Possible reasons for these effects are discussed.     

Biophysical studies on molecular mechanism of abortificient action of prostaglandins—IV. Conformation energy calculations on PGA1, PGB1 and PGE1

This paper reports the conformation energy (CE) calculations on three forms of prostaglandins (PGs) PGA₁, PGB₁ and PGE₁ on the basis of the empirical potential energy functions, for the simultaneous rotations around C₇–C₈ (θ), C₁₂–C₁₃ (β) and C₁₄–C₁₅ (β) bonds [Fig. 1(a)]. The isoenergy contours plotted for θβ rotations for the different β values show the existence of two low energy regions for thg equal to about 90° and 240° in all the three cases. The absolute minimum was obtained for thg = 240° and almost coincided with the crystallographic conformation for PGE₁ and PGA₁. In the case of PGB₁ series of low energy conformations were obtained with the thg values equal to about 90° and 270°, but none of them coincided with the observed crystallographic conformation. The paper discusses the comparison of the different low energy conformations in these three molecules, their biological relevance and the cause of disagreement in the case of PGB₁ with the crystallographic data.     

阿维菌素对南方小花蝽的毒杀作用

在室内条件下,研究了阿维菌素两种产品对南方小花蝽的毒杀效果.结果表明,两种产品对南方小花蝽不同虫态均有一定的毒杀作用.成虫、若虫对阿维菌素的敏感性基本相同,而卵的敏感性最低.阿维菌素对南方小花蝽除有直接的毒杀作用外,对其捕食能力也有一定影响.     

山地原花蝽核型研究(半翅目,花蝽科)

采用姬姆萨染色压片法,对花蝽科山地原花蝽性细胞进行了核型研究.结果表明该种单倍染色体组成为n=14A+XY,为无交叉减数分裂,性染色体经历后减数分裂,上述核型特征为阐明臭虫型内不同科的系统发育关系具有重要意义.     

金色蝗属一新种（直翅目：蝗总科）

本文记述在吉林省长白山,黑龙江省镜泊湖地区采到蝗虫－新种──长白山金色蝗Ｃｈｒｙｓａｃｒｉｓｃｈａｎｇｂａｉｓｈａｎｅｎｓｉｓｓｐ．ｎｏｖ,并附有特征图。     

朱砂藤根化学成分的研究

首次从朱砂藤根中人离并鉴定了β－香树脂醇乙本能酯,蒲公英醇,β－谷甾醇,另有两个结晶尚在鉴定中,将另文报道。     

小蹦蝗属二新种(直翅目:斑腿蝗亚科)

小蹦蝗属Pedopodisma Zheng,国内已知有3种,即秦岭小蹦蝗P.tsinlingensis,突眼小蹦蝗P.protrocula 和微翅小蹦蝗P.microptera。作者发现二新种。模式标本存放中国科学院动物研究所。本文量度单位均为毫米。     

方板蝗属一新种：（蝗总科：斑腿蝗科）

本文记述方板蝗属Squaroplatacris 1新种,小方板蝗Squaroplatacris elegans,sp.nov.。该新种近似于紫胫方板蝗S.violatibialis Liang et Zheng,主要区别为:体型较小,眼间距宽度狭于颜面隆起宽,前胸背板前缘平直,前胸背板后横沟位于背板中部或中后部,中胸腹板侧叶间中隔长为宽的1.2—1.3倍。     

拟大磨蚱属的分类研究(直翅目,蚱总科,短翼蚱科)(英文)

系统地研究拟大磨蚱属Macromotettixoides Zheng种类,共计有6种,其中有1新种,郑氏拟大磨蚱Zhengitettix zhengi sp.nov.。文中记述每个种的引证和分布地区,并附有分种检索表。郑氏拟大磨蚱,新种Zhengitettix zhengisp.nov.(图1~7)新种与本属其它已知种主要区别如下:前胸背板前缘平直;侧面观,前胸背板上缘近平直;缺前、后翅。模式标本保存于陕西师范大学动物研究所昆虫标本室。正模♀,广东梅县,雁洋(阴那山),海拔600m,2009-08-13,邓维安采。副模:4♂♂,12♀♀,同正模;1♀,福建武平(平川西山),2009-08-17,邓维安采;1♀,江西安远(三百山),海拔1000m,2009-08-19,邓维安采。词源:新种种名以郑哲民教授的姓氏命名,以示对郑教授在分类学领域做出突出贡献的敬意。     

浙江省小蹦蝗属一新种(直翅目:斑腿蝗科)

本文记述采自中国浙江省的小蹦蝗属１新种,即乌岩岭小蹦蝗Ｐｅｄｏｐｏｄｉｓｍａｗｕｙａｎｌｉｎｇｅｎｓｉｓ,ｓｐ．ｎｏｖ．。模式标本保存于山东大学生物系。     

消症汤对子宫内膜异位模型大鼠异位组织细胞增殖和凋亡的影响

目的　探讨“消症汤”对子宫内膜异位症 (endometriosis,EMT)模型大鼠异位组织细胞增殖和凋亡的影响 ,为临床用药提供实验依据。方法　制作大鼠EMT动物模型 ,模型鼠每日分别以 0 5ml和 1 0ml中药灌胃 ,连续用药 3周后观察异位组织细胞增殖指数和凋亡指数。结果　模型对照组细胞增殖指数大于用药组 ,凋亡指数小于用药组 (P <0 0 1)。 0 5ml组和 1 0ml组比较细胞增殖指数和凋亡指数无显著差异 (P >0 0 5 )。结论　“消症汤”能促进异位组织细胞凋亡 ,抑制异位组织增殖。     

云南省瘤股蚱属一新种记述(直翅目,枝背蚱科)

记述采自云南省金平县枝背蚱科Cladonotidae瘤股蚱属Tuberfemurus Zheng 1新种,郑氏瘤股蚱Tuberfemurus zhengi sp.nov.,模式标本保存于大理学院生命科学与化学学院.