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Transcriptional and posttranscriptional gene silencing are mechanistically related 总被引:30,自引:0,他引:30
Sijen T Vijn I Rebocho A van Blokland R Roelofs D Mol JN Kooter JM 《Current biology : CB》2001,11(6):436-440
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Transgene silencing in monocots 总被引:31,自引:0,他引:31
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A model for RNA-mediated gene silencing in higher plants 总被引:30,自引:0,他引:30
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Analysis of target sequences of DDM1s in Brassica rapa by MSAP 总被引:1,自引:0,他引:1
DNA methylation is an important epigenetic modification regulating gene expression and transposon silencing. Although epigenetic
regulation is involved in some agricultural traits, there has been relatively little research on epigenetic modifications
of genes in Brassica rapa, which includes many important vegetables. In B. rapa, orthologs of DDM1, a chromatin remodeling factor required for maintenance of DNA methylation, have been characterized and
DNA hypomethylated knock-down plants by RNAi (ddm1-RNAi plants) have been generated. In this study, we investigated differences of DNA methylation status at the genome-wide
level between a wild-type (WT) plant and a ddm1-RNAi plant by methylation-sensitive amplification polymorphism (MSAP) analysis. MSAP analysis detected changes of DNA methylation
of many repetitive sequences in the ddm1-RNAi plant. Search for body methylated regions in the WT plant revealed no difference in gene body methylation levels between
the WT plant and the ddm1-RNAi plant. These results indicate that repetitive sequences are preferentially methylated by DDM1 genes in B. rapa. 相似文献