Water relations and gas exchange in Fagus sylvatica L. and Quercus petraea (Mattuschka) Liebl. in a mixed stand at their southern limit of distribution in Europe

Water status and gas exchange of beech (Fagus sylvatica L.) and sessile oak [Quercus petraea (Mattuschka) Liebl.] were studied in a mixed stand in the Montejo de la Sierra forest (central Iberian Peninsula), one of the southernmost locations of both species in Europe. Gas exchange and water potential were measured in leaves at different canopy levels over several days in two growing seasons. The daily variation pattern was established with the measurements of three selected dates per year, representative of the soil moisture content situations in early, mid- and late summer. A similar daily time course of leaf water potential was found for the two species. Nevertheless, beech showed a most noticeable decrease of water potential at midmorning and maintained lower leaf water potential than oak in the early afternoon. In 1994 the sessile oak saplings showed higher values of predawn water potential (Ψ_pd) than beech at the end of summer, when soil moisture content was lowest (20 cm depth). Beech showed a significantly lower net assimilation rate (A) than sessile oak for leaves under the same PPFD. Maximum net photosynthesis values (A _max) for beech and sessile oak on sunny leaves were 10.1±0.4 μmol m^–2 s^–1 and 17.8±1.7 μmol m^–2 s^–1 respectively, and those for water vapour stomatal conductance (g _wv) were 265±31 mmol m^–2 s^–1 and 438±74 mmol m^–2 s^–1. Differences in A and g _wv between the two species were maintained throughout the day on all measurement dates. No clear relationship was found between water status of saplings and stomata performance; there was only a negative correlation between Ψ_pd and g _wvmid in beech. Nevertheless, a significant response to the air vapour pressure gradient between leaf and air was translated into stomata closure on an hourly basis, more intensively in beech. Received: 4 March 1999 / Accepted: 21 December 1999     

Seasonal changes in abundance and spatial distribution of the soil arthropods in a Japanese cedar (Cryptomeria japonica D.Don) plantation,with special reference to collembola and acarina

Seasonal changes in abundance and distribution pattern of soil micro-arthropods were studied in connection with a few environmental factors in a Japanese cedar (Cryptomeria japonica D. Don) plantation. The soil arthropods were sampled from three different depths at intervals of two months for two years. Of the collected animals (total 51000–155000 m⁻²), Collembola (20000–76000 m⁻²), oribatid mites (19000–55000 m⁻²) and carnivorous mites (6200–21000 m⁻²) were the numerically dominant animal groups. Low seasonal variations in abundance indicated their seasonal stability in population levels. The trends in seasonal fluctuation were similar among these groups and between the two years, showing bimodal pattern with little peaks in early summer and winter. The pattern of seasonal fluctuation in abundance of carnivorous mites (P _d) was significantly synchronized with that in the total abundance of Collembola and oribatid mites (P _τ). Thus, the number-ratios (P _d/P _τ) were fairly constant, ranging from 0.10 to 0.25. Seasonal changes in vertical distribution of the three animal groups showed a similar pattern for both years. The downward migrations were shown to be more affected by low temperatures in winter accompanied by snow coverage rather than by the desiccation of the surface soil in summer. All the three groups demonstrated as a whole slightly aggregated patterns of horizontal distribution throughout the two years. Temporal increases in the patchiness indices were observed from summer to autumn when the moisture content of the surface soil was low.     

Light response characteristics of a morphologically diverse group of southern hemisphere conifers as measured by chlorophyll fluorescence

Unlike northern hemisphere conifer families, the southern family, Podocarpaceae, produces a great variety of foliage forms ranging from functionally broad-, to needle-leaved. The production of broad photosynthetic surfaces in podocarps has been linked qualitatively to low-light-environments, and we undertook to assess the validity of this assumption by measuring the light response of a morphologically diverse group of podocarps. The light response, as apparent photochemical electron transport rate (ETR), was measured by modulated fluorescence in ten species of this family and six associated species (including five Cupressaceae and one functionally needle-leaved angiosperm) all grown under identical glasshouse conditions. In all species, ETR was found to increase as light intensity increased, reaching a peak value (ETR_max) at saturating quantum flux (PPFD_sat), and decreasing thereafter. ETR_max ranged from 217 μmol electrons · m⁻² · s⁻¹ at a PPFD_sat of 1725 μmol photons · m⁻² · s⁻¹ in Actinostrobus acuminatus to an ETR of 60 μmol electrons · m⁻² · s⁻¹ at a PPFD_sat of 745 μmol electrons · m⁻² · s⁻¹ in Podocarpus dispermis. Good correlations were observed between ETR_max and both PPFD_sat and maximum assimilation rate measured by gas-exchange analysis. The effective quantum yield at light saturation remained constant in all species with an average value of 0.278 ± 0.0035 determined for all 16 species. Differences in the shapes of light response curves were related to differences in the response of non-photochemical quenching (q _n), with q _n saturating faster in species with low PPFD_sat. Amongst the species of Podocarpaceae, the log of average shoot width was well correlated with PPFD_sat, wider leaves saturating at lower light intensities. This suggests that broadly flattened shoots in the Podocarpaceae are an adaptation to low light intensity. Received: 15 April 1996 / Accepted: 30 September 1996     

Effects of the halocline on water quality and phytoplankton composition in a shallow brackish lake (Lake Obuchi, Japan)

The relationships of the halocline to both water quality and phytoplankton composition in Lake Obuchi, a shallow brackish lake in northern Japan, were investigated from April 2001 to December 2004. The halocline in this lake became stronger in summer (July–September, mean maximum density gradient 4.3–5.8 ρ_tm⁻¹) but weaker in spring, fall, and winter (1.9–3.3 ρ_tm⁻¹). Although the difference in water quality between the upper and lower layers separated by the halocline was high in summer, nutrients (PO₄³⁻-P and NH₄⁺-N) were eluted from the bottom sediment as levels of dissolved oxygen decreased in the bottom layer because of the strong stratification caused by the halocline formed over the long term. Moreover, phytoplankton taxa composition also differed between the upper and lower layers in summer, but was similar in other seasons. The dominant phytoplankton taxa in the upper layer in summer were Skeletonema costatum and Cyclotella spp., whereas in the lower layer, Gymnodinium spp. (Dinophyceae) and Chlorophyceae, which prefer eutrophic and low dissolved oxygen conditions, dominated. This suggests that the halocline was related to differentiations in both water quality and ecosystem components between the upper and lower layers in the brackish lake water.     

Photosynthetic characteristics of dipterocarp seedlings in three tropical rain forest light environments: a basis for niche partitioning?

In three tropical rain forest light environments in Sabah, Malaysia, we compared photosynthesis in seedlings of ten climax tree species with putatively differing shade tolerances. The objectives of the study were (a) to characterise the range of photosynthetic responses in ten species of the Dipterocarpaceae and (b) to elucidate those photosynthetic characteristics that might provide a basis for niche partitioning. Seedlings were acclimated (c. 7 months) in three light environments; understorey, partial shade and a gap (140 m²). The light environments represented a gradation in median diurnal (0630–1830 hours) photon flux density (PFD) ranging from understorey (4.7 μmol m⁻² s⁻¹), through partial shade (21.2 μmol m⁻² s⁻¹) to gap (113.7 μmol m⁻² s⁻¹). Integrated diurnal PFD were in the sequence gap > partial shade > understorey (15.2, 4.7, 1.3 mol m⁻² day⁻¹, respectively). In gap-acclimated plants, species differed in the photosynthetic light-response variables apparent quantum yield, dark respiration rate, light compensation point, net saturated leaf assimilation rate (A _sat), and in stomatal conductance (g _s sat) when assimilation rate (A) was saturated. A light-demanding pioneer species (Macaranga hypoleuca) and a shade-demanding understorey species (Begonia sp.) had, respectively, higher and lower A _sat and g _s sat than the dipterocarp species. In high-light conditions A _sat and g _s sat were strongly positively correlated in dipterocarp species. Differing photosynthetic characteristics of gap-acclimated plants suggest that, in these dipterocarp species, different rates of carbon fixation may be an important factor contributing towards niche partitioning. Mean integrated diurnal A (A _diurnal) in the gap, partial shade and understory were, respectively, 122.9, 52.7, 20.5 mmol m⁻² day⁻¹. Differences occurred in A _diurnal of dipterocarp species between light environments. When Macaranga was included, differences in A _diurnal were evident in the gap and partial shade, and in both cases were attributed to the pioneer. For the variable A _diurnal, there was of a shift in the rank position of Macaranga among light environments, but a shift did not occur among the dipterocarp species. Results from this study are consistent with the idea that rates of carbon fixation per unit leaf area may contribute towards niche differentiation between the climax and single pioneer species, but not within the group of climax species. Other physiological and/or carbon allocation factors may be involved in any niche partitioning; dipterocarp species often have inherently different growth rates and susceptibility to herbivory. As an alternative to niche partitioning, dipterocarp species may co-exist in natural light environments as a result of habitat disequilibrium or purely stochastic processes. Received: 2 April 1997 / Accepted: 13 July 1997     

Photosynthesis of a temperate fallow C<Subscript>3</Subscript> herbaceous ecosystem: measurements and model simulations at the leaf and canopy levels

The objectives of the study were to characterize photosynthesis of temperate fallow C₃herbaceous species and examine the performance of a simple photosynthesis model (based on the Farquhar’s equations) to simulate carbon fluxes at the leaf and canopy levels. The maximum rate of carboxylation at 25°C (V _m0) was estimated for sunlit leaves using in situ gas exchange data under saturating irradiance. Throughout the seasons, leaf measurements indicate that values of V _m0 were similar for the four major species of the fallow. The rate declined from March (100 μmol m⁻² s⁻¹) to July (50 μmol m⁻² s⁻¹) and remained almost constant until November. The maximum quantum yield estimated for Potentilla reptans L. (dominant species) was 0.082 mol(CO₂) mol⁻¹(photon absorbed), similar to values already published for C₃ species. Leaf area index (LAI) increased from winter (less than 0.2 m² m⁻²) to spring (up to 4 m² m⁻²). Rates of canopy photosynthesis (measured with a canopy chamber) strongly depended on LAI and temperature, in addition to irradiance. They reached a maximum of 25 μmol m⁻² s⁻¹ and were intermediate between those published for C₄ grassland or cultivated species, and on woody species. At leaf level, simulations gave realistic predictions. At canopy level, the model had the ability to reproduce the effects of environmental and seasonal conditions. However, simulations underestimated the photosynthetic activity of the fallow canopy.     

Can light-saturated photosynthesis in lowland tropical forests be estimated by one light level?

Leaf-level net photosynthesis (A_n) estimates and associated photosynthetic parameters are crucial for accurately parameterizing photosynthesis models. For tropical forests, such data are poorly available and collected at variable light conditions. To avoid over- or underestimation of modeled photosynthesis, it is critical to know at which photosynthetic photon flux density (PPFD) photosynthesis becomes light-saturated. We studied the dependence of A_n on PPFD in two tropical forests in French Guiana. We estimated the light saturation range, including the lowest PPFD level at which A_sat (A_n at light saturation) is reached, as well as the PPFD range at which A_sat remained unaltered. The light saturation range was derived from photosynthetic light-response curves, and within-canopy and interspecific differences were studied. We observed wide light saturation ranges of A_n. Light saturation ranges differed among canopy heights, but a PPFD level of 1,000 µmol m⁻² s⁻¹ was common across all heights, except for pioneer trees species that did not reach light saturation below 2,000 µmol m⁻² s⁻¹. A light intensity of 1,000 µmol m⁻² s⁻¹ sufficed for measuring A_sat of climax species at our study sites, independent of the species or the canopy height. Because of the wide light saturation ranges, results from studies measuring A_sat at higher PPFD levels (for upper canopy leaves up to 1,600 µmol m⁻² s⁻¹) are comparable with studies measuring at 1,000 µmol m⁻² s⁻¹.     

Winter and summer nitrous oxide and nitrogen oxides fluxes from a seasonally snow-covered subalpine meadow at Niwot Ridge,Colorado

The soil emission rates (fluxes) of nitrous oxide (N₂O) and nitrogen oxides (NO + NO₂ = NO _x ) through a seasonal snowpack were determined by a flux gradient method from near-continuous 2-year measurements using an automated system for sampling interstitial air at various heights within the snowpack from a subalpine site at Niwot Ridge, Colorado. The winter seasonal-averaged N₂O fluxes of 0.047–0.069 nmol m⁻² s⁻¹ were ~15 times higher than observed NO _x fluxes of 0.0030–0.0067 nmol m⁻² s⁻¹. During spring N₂O emissions first peaked and then dropped sharply as the soil water content increased from the release of snowpack meltwater, while other gases, including NO _x and CO₂ did not show this behavior. To compare and contrast the winter fluxes with snow-free conditions, N₂O fluxes were also measured at the same site in the summers of 2006 and 2007 using a closed soil chamber method. Summer N₂O fluxes followed a decreasing trend during the dry-out period after snowmelt, interrupted by higher values related to precipitation events. These peaks were up to 2–3 times higher than the background summer levels. The integrated N₂O-N loss over the summer period was calculated to be 1.1–2.4 kg N ha⁻¹, compared to ~0.24–0.34 kg N ha⁻¹ for the winter season. These wintertime N₂O fluxes from subniveal soil are generally higher than the few previously published data. These results are of the same order of magnitude as data from more productive ecosystems such as fertilized grasslands and high-N-cycling forests, most likely because of a combination of the relatively well-developed soils and the fact that subnivean biogeochemical processes are promoted by the deep, insulating snowpack. Hence, microbially mediated oxidized nitrogen emissions occurring during the winter can be a significant part of the N-cycle in seasonally snow-covered subalpine ecosystems.     

Offsetting high water demands with high productivity: Sorghum as a biofuel crop in a high irradiance arid ecosystem

High irradiance arid environments are promising, yet understudied, areas for biofuel production. We investigated the productivity and environmental trade‐offs of growing sorghum (Sorghum bicolor) as a biofuel feedstock in the low deserts of California (CA). Using a 5.3 ha experimental field in the Imperial Valley, CA, we measured aboveground biomass production and net ecosystem exchange of CO₂ and H₂O via eddy covariance over three growing periods between February and November 2012. Environmental conditions were extreme, with high irradiance, vapor pressure deficit (VPD), and air temperature throughout the growing season. Air temperature peaked in August with a maximum of 45.7 °C. Sorghum produced an annual aboveground biomass yield of 43.7 Mg per hectare. Net ecosystem exchange (NEE) was highest during the summer growth period and reached a maximum of ?68 μmol CO₂ m^?2 s^?1. Water use efficiency, or biomass water ratio (BWR), was high (4.0 g dry biomass kg^?1 H₂O) despite high seasonal evapotranspiration (1094 kg H₂O m^?2). The BWR of sorghum surpassed that of many C4 biofuel candidate crops in the United States, as well as that of alfalfa which is currently widely grown in the Imperial Valley. Sorghum also outperformed many US biofuel crops in terms of radiation use efficiency (RUE), achieving 1.5 g dry biomass MJ^?1. We found no evidence of saturation of NEE at high levels of photosynthetically active radiation (PAR) (up to 2250 μmol m^?2 s^?1). In addition, we found no evidence that NEE was inhibited by either high VPD or air temperature during peak photosynthetic phases. The combination of high productivity, high BWR, and high RUE suggests that sorghum is well adapted to this extreme environment. The biomass production rates and efficiency metrics spanning three growing periods provide fundamental data for future Life Cycle Assessments (LCA), which are needed to assess the sustainability of this sorghum biofuel feedstock system.     

Seasonal changes in canopy photosynthesis and foliage respiration in a Rhizophora stylosa stand at the northern limit of its natural distribution

Gross photosynthesis and respiration rates of leaves at different canopy heights in a Rhizophora stylosa Griff. stand were measured monthly over 1 year at Manko Wetland, Okinawa Island, Japan, which is the northern limit of its distribution. The light-saturated net photosynthesis rate for the leaves at the top of the canopy showed a maximum value of 17 μmol CO₂ m⁻² s⁻¹ in warm season and a minimum value of 6 μmol CO₂ m⁻² s⁻¹ in cold season. The light-saturated gross photosynthesis and dark respiration rates of the leaves existing at the top of the canopy were 2−7 times and 3–16 times, respectively, those of leaves at the bottom of the canopy throughout the year. The light compensation point of leaves showed maximum and minimum peaks in warm season and cold season, respectively. The annual canopy gross photosynthesis, foliage respiration, and surplus production were estimated as 117, 49, and 68 t CO₂ ha⁻¹ year⁻¹, respectively. The energy efficiency of the annual canopy gross photosynthesis was 2.5%. The gross primary production GPP fell near the regression curve of GPP on the product of leaf area index and warmth index, the regression curve which was established for forests in the Western Pacific with humid climates.     

Can water responses in Stipa tenacissima L. during the summer season be promoted by non-rainfall water gains in soil?

This article reports on quantified soil water gains and their possible effects on summer water relationships in a semiarid Stipa tenacissima L. grasslands located in SE Spain. We believe that the net soil water gains detected using minilysimeters could be from soil water vapour adsorption (WVA). Our study of high water-stress showed stomatal conductance (21.8–43.1 mmol H₂O m⁻² s⁻¹) in S. tenacissima leaves unusual for the summer season, and the evapotranspiration from S. tenacissima grassland, estimated by a multi-source sparse evapotranspiration model, closely corresponding to total WVA. This highlights the importance of summer soil WVA to stomatal conductance and vital transpiration in S. tenacissima. This study measured pre-dawn leaf water potential (ψ) response to sporadic light rainfall, finding that a light summer rainfall (1.59 mm day⁻¹) was sufficient to vary ψ in S. tenacissima from −3.8 (close to the turgour loss point) to −2.7 MPa. We hypothesize that soil WVA can supply vegetation with water vital to its survival in seasons with a severe water deficit, giving rise to a close relationship between soil water dynamics and plant water response.     

Arthropod communities in a Japanese cedar (Cryptomeria japonica D. Don) plantation: Abundance,biomass and some properties

Arboreal arthropod communities were censused by insecticidal knockdown in a plantation of Japanese cedar,Cryptomeria japonica, in central Japan from April 1983 to February 1985 at intervals of two months. All arthropods sampled were allocated to higher taxonomic groups or guilds and the composition of the communities was analyzed with respect to abundance, biomass and their seasonal trends. The total densities fluctuated seasonally from 200 to 3500 m⁻²; corresponding biomass values ranged from 7 to 600 mg fresh weight m⁻². Maximum density and biomass generally occurred in summer and were minimum in winter. The consistently dominant guild in terms of abundance were the detritivores, mostly comprising Collembola and oribatid mites, accounting for 36–93% of all arthropods; phytophages, predators and detritivores were major guilds in terms of biomass, showing average proportions of 27%, 23% and 20%, respectively. Biomass ratios of predators to prey were generally high in some canopy communities, suggesting the importance of predation pressure in regulating the population levels of arthropods in forests. Average individual size of predators was approximately proportional to that of prey, irrespective of tree locality. Soil arthropod communities maintained densities approximately 10²–10³ times as large as the corresponding canopy communities throughout the year. The seasonal variations in abundance were much greater in canopy than in soil communities.     

Carbon balance and water use efficiency of frequently cut Lolium perenne L. swards at elevated carbon dioxide

The impact of doubled atmospheric [CO₂] on the carbon balance of regularly cut Lolium perenne L. swards was studied for two years under semi-field conditions in the Wageningen Rhizolab. CO₂ and H₂O vapour exchange rates of the swards were measured continuously for two years in transparent enclosures. The light utilization efficiencies of the swards ranged between 1.5 g CO₂ MJ^–1 global radiation (high light, ambient [CO₂]) and 2.8 g CO₂ MJ^–1 (low light, doubled [CO₂]). The above-ground net primary productivity (NPP) in the enclosures was greater by 29% in 1994 and 43% in 1995 in the doubled [CO₂] treatments, but only 20% and 25% more carbon was recovered in the periodical cuts. Thus, NPP increased significantly more than did the harvested above-ground biomass. The positive [CO₂] effect on net carbon assimilation is therefore associated with a preferential allocation of extra carbon to the roots and soil. In addition to higher canopy photosynthesis and leaf elongation rates, a small part of the positive [CO₂] effects on NPP could be attributed to a decrease of the specific respiration of the shoots. On a canopy basis however, respiration was equal or slightly higher at doubled [CO₂] due to the higher amount of standing biomass. Comparison of NPP and carbon recovered in different harvests showed that allocation to roots and soil was highest in spring, it was low in early summer and increased again in late summer and autumn. The total gross amount of carbon partitioned to the roots and soil during the two year period was 57% more at doubled [CO₂]. The total amount of carbon that was sequestered in the soil after subtraction of the respiratory losses was 458 g m^–2 and 779 g m^–2 in the ambient and doubled [CO₂] treatments, respectively. The average water use efficiency (WUE) of the swards was increased by a factor 1.5 at doubled [CO₂]. Both WUE and its positive interaction with [CO₂] varied between years and were positively correlated with global irradiance. At doubled [CO₂], the higher WUE was fully compensated for by a higher leaf area index. Therefore, total transpiration on a canopy basis was equal for the ambient and the doubled [CO₂] concentrations in both years.     

Effects of water stress on gas exchange of field grown <Emphasis Type="Italic">Zea mays</Emphasis> L. in Southern Italy: an analysis at canopy and leaf level

Zea mays is cultivated in the Mediterranean regions where summer drought may lead to photoinhibition when irrigation is not available. In this work the response of maize to water stress was evaluated by gas exchange measurements at the canopy and leaf level. Leaf gas exchange was assessed before, during and after water stress, while canopy turbulent fluxes of mass and energy were performed on a continuous basis. In the early growth period, a linear increment of net ecosystem photosynthetic rate (P _NE) to incoming of photosynthetic photon flux density (PPFD) was found and net leaf photosynthetic rate (P _NL) showed the tendency to saturate under high irradiance. During water stress, the relationship between P _NE and PPFD became curvilinear and both P _NE and P _NL saturated in a range between 1,000 and 1,500 μmol (photons) m⁻² s⁻¹. Leaf water potential (ψ_l) dropped from −1.50 to −1.88 MPa during water stress, indicating that leaf and canopy gas exchanges were limited by stomatal conductance. With the restoration of irrigation, P _NE, P _NL and ψ_l showed a recovery, and P _NE and P _NL reached the highest values of whole study period. Leaf area index (LAI) reached a value of 3.0 m² m⁻². The relationship between P _NE and PPFD remained curvilinear and P _NE values were lower than those of a typical well-irrigated maize crop. The recovery in P _NE and P _NL after stress, and ψ_l values during stress indicate that the photosynthetic apparatus was not damaged while soil moisture stress after-effects resulted in a sub-optimal LAI values, which in turn depressed P _NE.     

Dynamics of soil respiration in sparse <Emphasis Type="Italic">Ulmus pumila</Emphasis> woodland under semi-arid climate

Sparse Ulmus pumila woodlands play an important role in contributing to ecosystem function in semi-arid grassland of northern China. To understand the key attributes of soil carbon cycling in U. pumila woodland, we studied dynamics of soil respiration in the canopy field (i.e., the projected crown cover area) and the open field at locations differing in distance (i.e., at 1–1.5, 3–4, 10, and >15 m) to tree stems from July through September of 2005, and measured soil biotic factors (e.g., fine root mass, soil microbial biomass, and activity) and abiotic factors [e.g., soil water content (SWC) and organic carbon] in mid-August. Soil respiration was further separated into root component and microbial component at the end of the field measurement in September. Results showed that soil respiration had a significant exponent relationship with soil temperature at 10-cm depth. The temperature sensitivity index of soil respiration, Q ₁₀, was lower than the global average of 2.0, and declined significantly (P < 0.05) with distance. The rate of soil respiration was generally greater in the canopy field than in the open field; monthly mean of soil respiration was 305.5–730.8 mg CO₂ m⁻² h⁻¹ in the canopy field and 299.6–443.1 mg CO₂ m⁻² h⁻¹ in the open field from July through September; basal soil respiration at 10°C declined with distance, and varied from ~250 mg CO₂ m⁻² h⁻¹ near tree stems to <200 mg CO₂ m⁻² h⁻¹ in the open field. Variations in soil respiration with distance were consistent with patterns of SWC, fine root mass, microbial biomass and activities. Regression analysis indicated that soil respiration was tightly coupled with microbial respiration and only weakly related to root respiration. Overall, variations in SWC, soil nutrients, microbial biomass, and microbial activity are largely responsible for the spatial heterogeneity of soil respiration in this semi-arid U. pumila woodland.     

Influence of contrasting availabilities of water and nutrients on the radiation use efficiency in C3 and C4 grasses

The radiation use efficiency (RUE) model is one of the most used tools to generate large spatial and temporal scale net primary productivity (NPP) estimations by remote sensing. It involves two key issues to make accurate estimations of NPP: the estimation of the fraction of photosynthetically active radiation (PAR) intercepted by vegetation (fPAR) and the estimation of the plant RUE. The objectives of this work were to quantify the above‐ground RUE under optimal water and nutrient conditions in two C₃ and one C₄ grass species and to analyse the effect of restrictions in these factors upon RUE by comparing both metabolic pathways. Grasses were cultivated from seeds and four treatments combining contrasting availabilities of water and nutrients were applied. RUE values were calculated from measurements of the incoming PAR, fPAR and productivity. In each of the species, plants with sufficient water and nutrients showed the highest RUE (2.61–3.52 g MJ^?1), whereas those with deficiencies in both resources presented the lowest RUE (1.15–2.39 g MJ^?1). Cynodon dactylon (C₄) was the species with higher value of RUE and no significant differences were detected between treatments. However, no significant differences were detected between C. dactylon and D. glomerata under no stress treatment (N1W1) and between C. dactylon and L. perenne under water stress treatment (N1W0). RUE values of Dactylis glomerata (C₃) diminished if only one of the two stress factors was presented, while Lolium perenne (C₃) only when both stress factors were present. The decreases under stress treatments were between 35% and 60% compared with the no stress treatment. When regional NPP is estimated it is therefore important to take into account the decrease in the RUE, especially in areas under severe stress.     

Moisture adsorption capacity and surface area as deduced from vapour pressure isotherms in relation to hygroscopic water of soils

Six calcareous and alluvial soil profiles differing in their texture, CaCO₃ and salinity were chosen from west and middle Nile Delta for the present study. The 1^st and 2^nd profiles from Borg El-Arab area were sandy loam in texture and > 30% CaCO₃, while the 3^rd and 4^th profiles (from Nubaria area) were sandy clay loam and < 30% CaCO₃. The 2^nd and 4^th profiles were taken from cultivated area with maize. The 5^th profile from Epshan area was non-saline clay alluvial soil and the 6^th from El-Khamsen was saline clay alluvial soil. The relation between soil moisture content (W%) and water vapour pressure (P/P _o) was established for the mentioned soils. Data showed that the specific surface area (S) values were 34–53 and 44–60 m²/g for calcareous soils of Borg El-Arab and Nubaria areas, 206–219 and 206–249 m²/g for non-saline and saline clay alluvial soils of Epshan and El-Khamsen areas, respectively. The corresponding values of the external specific surface area (S _e) were 16–21, 14–22, 72–86 and 92–112 m²/g. Submitting W _m+W _me as an adsorption boundary of moisture films (W _c) (where W _m is mono-adsorbed layer of water vapour on soil particles and W _me is the external mono-adsorbed layer), the maximum water adsorption capacity (W _a) was found to be W _c + W _me or W _m + 2W _me. It was ranged from 1.88 to 2.70%, 1.97 to 2.95%, 9.70–10.70% and 10.80 to 13.12% while the maximum hygroscopic water (M _H) values were 2.43–3.78%, 2.91–4.65%, 16–17% and 18.30–21.9% for the studied soil profiles respectively. The residual moisture content (θ _r) at pF 7 and P/P _o = 0 was ranged from 0.0005–0.0010%, 0.0007–0.0019% and 0.0043–0.0048% in Borg El-Arab, Nubaria and Epshan soil profiles, respectively. The inter-relations between the surface area and the hygroscopic moisture parameters of the soils under investigation were as follows Calcareous soils; W _m = 0.40 M _H, W _c = 0.55 M _H, W _a = 0.70 M _H, S = 14 M _H Non-saline soil; W _m = 0.35 M _H, W _c = 0.49 M _H, W _a = 0.63 M _H, S = 13 M _H Saline soil; W _m = 031 M _H, W _c = 0.45 M _H, W _a = 0.59 M _H, S = 12 M _H These relations give possibility to deduce the soil moisture adsorption capacities and specific surface area via maximum hygroscopic water, which can be obtained through the experimental determination of water vapor adsorption isotherms.     

Transpiration and whole-tree conductance in ponderosa pine trees of different heights

Changes in leaf physiology with tree age and size could alter forest growth, water yield, and carbon fluxes. We measured tree water flux (Q) for 14 ponderosa pine trees in two size classes (12 m tall and ∼40 years old, and 36 m tall and ∼ 290 years old) to determine if transpiration (E) and whole-tree conductance (g _t) differed between the two sizes of trees. For both size classes, E was approximately equal to Q measured 2 m above the ground: Q was most highly correlated with current, not lagged, water vapor pressure deficit, and night Q was <12% of total daily flux. E for days 165–195 and 240–260 averaged 0.97 mmol m^–2 (leaf area, projected) s^–1 for the 12-m trees and 0.57 mmol m^–2 (leaf area) s^–1 for the 36-m trees. When photosynthetically active radiation (I _P) exceeded the light saturation for photosynthesis in ponderosa pine (900 μmol m^–2 (ground) s^–1), differences in E were more pronounced: 2.4 mmol m^–2 (leaf area) s^–1 for the 12-m trees and 1.2 mmol m^–2 s^–1 for the 36-m trees, yielding g _t of 140 mmol m^–2 (leaf area) s^–1 for the 12-m trees and 72 mmol m^–2 s^–1 for the 36-m trees. Extrapolated to forests with leaf area index =1, the 36-m trees would transpire 117 mm between 1 June and 31 August compared to 170 mm for the 12-m trees, a difference of 15% of average annual precipitation. Lower g _t in the taller trees also likely lowers photosynthesis during the growing season. Received: 19 April 1999 / Accepted: 23 March 2000     

Small scale vertical gradients of Arctic ice algal photophysiological properties

Photosynthetic parameters of phytoplankton and sea ice algae from landfast sea ice of the Chukchi Sea off Point Barrow, Alaska, were assessed in spring 2005 and winter through spring 2006 using Pulse Amplitude Modulated (PAM) fluorometry including estimates of maximum quantum efficiency (F _v/F _m), maximum relative electron transport rate (rETR_max), photosynthetic efficiency (α), and the photoadaptive index (E _k). The use of centrifuged brine samples allowed to document vertical gradients in ice algal acclimation with 5 cm vertical resolution for the first time. Bottom ice algae (0–5 cm from ice–water interface) expressed low F _v/F _m (0.331–0.426) and low α (0.098–0.130 (μmol photons m⁻²s⁻¹)⁻¹) in December. F _v/F _m and α increased in March and May (0.468–0.588 and 0.141–0.438 (μmol photons m⁻²s⁻¹)⁻¹, respectively) indicating increased photosynthetic activity. In addition, increases in rETR_max (3.3–16.4 a.u.) and E _k (20–88 μmol photons m⁻² s⁻¹) from December to May illustrates a higher potential for primary productivity as communities become better acclimated to under-ice light conditions. In conclusion, photosynthetic performance by ice algae (as assessed by PAM fluorometry) was tightly linked to sea ice salinity, temperature, and inorganic nutrient concentrations (mainly nitrogen).     

Estimating heat tolerance among plant species by two chlorophyll fluorescence parameters

The heat tolerance of 8 temperate- and 1 subtropical-origin C₃ species as well as 17 tropical-origin ones, including C₃, C₄, and CAM species, was estimated using both F₀-T curve and the ratio of chlorophyll fluorescence parameters, prior to and after high temperature treatment. When leaves were heated at the rate of ca. 1 °C min⁻¹ in darkness, the critical temperature (T_c) varied extensively among species. The T_c's of all 8 temperate-origin species ranged between 40–46 °C in winter (mean temperature 16–19 °C), and between 32–48 °C in summer (mean temperature ca. 30 °C). Those for 1 subtropical- and 12 tropical-origin C₃ species ranged between 25–44 °C and 35–48 °C, and for 1 CAM and 4 C₄ species were 41–47 and 45–46 °C, respectively. Acclimating three C₃ herbaceous plants at high temperature (33/28 °C, day/night) for 10 d in winter caused their T_c's rising to nearly the values measured in summer. When leaves were exposed to 45 °C for 20 min and then kept at room temperature in darkness for 1 h, a significant correlation between RF_v/m (the ratio of F_v/F_m before and after 45 °C treatment) and T_c was observed for all tested temperate-origin C₃ species as well as tropical-origin CAM and C₄ species. However, F₀ and F_v/F_m of the tropical-origin C₃ species were less sensitive to 45 °C treatment, regardless of a large variation of T_c; thus no significant correlation was found between their RF_v/m and T_c. Thus T_c might not be a suitable index of heat tolerance for plants with wide range of environmental adaptation. Nevertheless, T_c's of tropical origin C₃ species, varying and showing high plasticity to seasonal changes and temperature treatment, appeared suitable for the estimation of the degree of temperature acclimation in the same species.