Implications of Male and Female Contributions to Sexual Size Dimorphism for Inferring Behavior in the Hominin Fossil Record

Sexual dimorphism is commonly used to directly infer or support reconstructions of social behavior in early hominins. This is often done by comparing the magnitude of sexual size dimorphism to that seen in extant primates and extrapolating a likely social behavior. Such comparisons are of limited value, though, allowing only the inference of strong male–male competition when dimorphism is strong. Recent studies have begun to focus on the selective factors that impact female body size, and thereby size dimorphism. Considerations of changes in male and female size in the fossil record potentially allow insight into the meaning of changes in sexual dimorphism through time. To illustrate, I compare estimates of body mass dimorphism for four hominin taxa to assess changes in male and female size. Assuming that early Homo represents a single taxon, sexual size dimorphism increased in early Homo through an increase in male size, but was subsequently reduced through an increase in female size in Homo erectus. This would imply a significant increase in sexual selection acting on males in early Homo. An increase in female size with a loss of dimorphism in Homo erectus would imply a simultaneous shift in female optimal body size through selection for increased female fecundity, and/or an increase in female resource abundance, coupled with a shift in selection acting on male size. Although none of these inferences are certain, the exercise illustrates the potential for considering how dimorphism changes through time, rather than simply focusing on the magnitude of size dimorphism in isolation.     

Mechanisms of Transient Signaling via Short and Long Prolactin Receptor Isoforms in Female and Male Sensory Neurons

Prolactin (PRL) regulates activity of nociceptors and causes hyperalgesia in pain conditions. PRL enhances nociceptive responses by rapidly modulating channels in nociceptors. The molecular mechanisms underlying PRL-induced transient signaling in neurons are not well understood. Here we use a variety of cell biology and pharmacological approaches to show that PRL transiently enhanced capsaicin-evoked responses involve protein kinase C ϵ (PKCϵ) or phosphatidylinositol 3-kinase (PI3K) pathways in female rat trigeminal (TG) neurons. We next reconstituted PRL-induced signaling in a heterologous expression system and TG neurons from PRL receptor (PRLR)-null mutant mice by expressing rat PRLR-long isoform (PRLR-L), PRLR-short isoform (PRLR-S), or a mix of both. Results show that PRLR-S, but not PRLR-L, is capable of mediating PRL-induced transient enhancement of capsaicin responses in both male and female TG neurons. However, co-expression of PRLR-L with PRLR-S (1:1 ratio) leads to the inhibition of the transient PRL actions. Co-expression of PRLR-L deletion mutants with PRLR-S indicated that the cytoplasmic site adjacent to the trans-membrane domain of PRLR-L was responsible for inhibitory effects of PRLR-L. Furthermore, in situ hybridization and immunohistochemistry data indicate that in normal conditions, PRLR-L is expressed mainly in glia with little expression in rat sensory neurons (3–5%) and human nerves. The predominant PRLR form in TG neurons/nerves from rats and humans is PRLR-S. Altogether, PRL-induced transient signaling in sensory neurons is governed by PI3K or PKCϵ, mediated via the PRLR-S isoform, and transient effects mediated by PRLR-S are inhibited by presence of PRLR-L in these cells.     

Differential Sperm Priming by Male Sailfin Mollies (Poecilia latipinna): Effects of Female and Male Size

Recent interest in sperm competition has led to a re‐evaluation of the ‘cheap sperm’ assumption inherent in many studies of sexual selection. In particular, mounting evidence suggests that male sperm availability can be increased by the presence of females. However, there is little information on how this interacts with male traits presumably affected by female mate choice, such as larger size. This study examines the effects on male sperm availability of female presence, male body size, and female body size in the sailfin molly, Poecilia latipinna. Individual males of variable body sizes were isolated in divided tanks for 3 d, after which time either a female or no female was added to the other side of the tank. Prior to the treatments, larger males had more stripped sperm than smaller males. Female presence significantly increased the amount of sperm males primed, but this effect was strongest in small males. Furthermore, males showed a greater priming response in the presence of larger females than in the presence of smaller females. These results demonstrate that the presence of sexually mature females increases the amount of sperm males have for insemination. Furthermore, traits that indicate female fecundity may be used by males as cues in male mate choice.     

The Landscape of Candidate Driver Genes Differs between Male and Female Breast Cancer

The rapidly growing collection of diverse genome-scale data from multiple tumor types sheds light on various aspects of the underlying tumor biology. With the objective to identify genes of importance for breast tumorigenesis in men and to enable comparisons with genes important for breast cancer development in women, we applied the computational framework COpy Number and EXpression In Cancer (CONEXIC) to detect candidate driver genes among all altered passenger genes. Unique to this approach is that each driver gene is associated with several gene modules that are believed to be altered by the driver. Thirty candidate drivers were found in the male breast cancers and 67 in the female breast cancers. We identified many known drivers of breast cancer and other types of cancer, in the female dataset (e.g. GATA3, CCNE1, GRB7, CDK4). In contrast, only three known cancer genes were found among male breast cancers; MAP2K4, LHP, and ZNF217. Many of the candidate drivers identified are known to be involved in processes associated with tumorigenesis, including proliferation, invasion and differentiation. One of the modules identified in male breast cancer was regulated by THY1, a gene involved in invasion and related to epithelial-mesenchymal transition. Furthermore, men with THY1 positive breast cancers had significantly inferior survival. THY1 may thus be a promising novel prognostic marker for male breast cancer. Another module identified among male breast cancers, regulated by SPAG5, was closely associated with proliferation. Our data indicate that male and female breast cancers display highly different landscapes of candidate driver genes, as only a few genes were found in common between the two. Consequently, the pathobiology of male breast cancer may differ from that of female breast cancer and can be associated with differences in prognosis; men diagnosed with breast cancer may consequently require different management and treatment strategies than women.     

The Evolution of Reversed Sexual Size Dimorphism in Hawks,Falcons and Owls: A Comparative Study

Many hypotheses have been proposed to account for the origin and maintenance of reversed size dimorphism (RSD, females being larger than males) in hawks, falcons and owls, but no consensus has been reached. I performed comparative analyses, using both cross-taxa data and phylogenetically independent contrasts, to investigate potential correlates of reversed size dimorphism. Using a similar set of explanatory variables, covering morphology, life history and ecology, I tested whether any trait coevolved with size dimorphism in all three groups and hence provided a general explanation for the evolution of RSD. For hawks, strong correlates were found in the foraging-variable complex, so RSD might have evolved in species hunting large and agile prey. This is consistent with the intersexual-competition hypothesis (sexes have evolved different sizes to lessen intersexual competition for food), but especially the small-male hypothesis (males have evolved to be smaller to be more efficient foragers). Evolutionary pathway analyses suggest that RSD evolved most likely as a precursor of changes in hunting strategy but as a consequence of high reproduction. The falcons showed a similar pattern: species with strong RSD hunted larger and more agile prey. The evolutionary pathway analysis supported the idea that RSD evolved before the specialisation on more agile and/or larger prey. Finally for owls, the results showed clear parallels. RSD increased with prey size, consistent with the small-male hypothesis. Evolutionary pathway analysis suggests that RSD in owls has most likely evolved before specialisation on large prey, so a small and more agile male might be advantageous even when hunting small prey. These results suggest that RSD in hawks, falcons and owls evolved due to natural-selection pressures rather than sexual-selection pressures. Co-ordinating editor: J. Tuomi     

花生雌雄两性细胞发育关系的研究

本试验结果表明 ,雌雄配子体形成过程和程序与前人结论相一致 ,但发现 :1 )尽管雌雄蕊同时形成 ,但在雌雄配子形成不同步的情况下 ,花粉和胚囊中的大量淀粉却能同时充实 ,并且同时成熟等待受精。 2 )大孢子母细胞形成比小孢子母细胞迟 0 .7～ 0 .8片展叶 ;雌配子体形成比雄配子体迟 0 .5～ 0 .6片展叶。 3)雌雄两性细胞发育特点不同 ,雄性是小孢子形成快而花粉粒发育慢 ,雌性是大孢子形成慢而胚囊发育快 ,为此在所用时间上 ,花粉粒发育约是小孢子形成的 1 .6倍 ,是胚囊发育的 2 .3倍 ,而大孢子形成约是胚囊发育的 2 .2倍 ,约是小孢子形成的 1 .4倍。     

Lack of Evolution of Sexual Size Dimorphism in Heteromyidae (Rodentia): The Influence of Resource Defense and the Trade-Off between Pre- and Post-Copulatory Trait Investment

One paradoxical finding in some mammals is the presence of male–male intrasexual competition in the absence of sexual size dimorphism. It has been a major goal of evolutionary biologists for over a century to understand why some species in which large males can monopolize multiple mates while excluding smaller competitors, exhibit little or no sexual dimorphism. In this paper I examine three of the main hypotheses that have been proposed to explain this conundrum using as study case the Heteromyidae, a rodent family with subtle sexual size dimorphism. Using a phylogenetic comparative approach, I address the potential influence of (1) fecundity selection, (2) covariation between pre- and post-copulatory traits, and (3) environmental constraints (resource shortage) in explaining patterns of body size and sexual size dimorphism (SSD) across 62 heteromyid species. Baculum size, a proxy of the strength of post-copulatory sexual selection, and SSD were negatively correlated suggesting that heteromyid rodents balance their reproductive investment between pre- and post-copulatory traits, which may prevent the evolution of extensive SSD. Results also support a role for resource competition in moderating SSD. The amount of SSD correlated negatively with latitude. This can be explained if high productivity relaxes the level of intrasexual competition among females, leading to more male-biased dimorphism since forces acting on both sexes are not cancelled. In line with this argument, territorial species exhibited a higher dimorphism in comparison with social species. No support was found for the fecundity selection hypothesis. Overall, this study provides insight into the factors driving observed patterns of sexual dimorphism in this iconic group and highlights the need to consider a broader framework beyond sexual selection for better understanding the evolution of dimorphism in this family.     

The Long and Short of Hearing in the Mosquito Aedes aegypti

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胡杨雌雄株叶片的比较解剖学研究

应用石蜡切片法对胡杨(Populus euphratica Oliv.)雌、雄株叶片进行比较解剖研究,结果表明:(1)胡杨雌、雄株叶片都由表皮、叶肉与叶脉构成,表皮由2层细胞构成复表皮;上、下表皮内均分化出了2～3层栅栏组织细胞,栅栏组织之间有少量海绵组织细胞;主脉维管束通常1个,由木质部、韧皮部、少量形成层及类似禾本...     

Testosterone Manipulation of Male Attractiveness has no Detectable Effect on Female Home-Range Size and Behavior During the Fertile Period

Female dark‐eyed juncos (Junco hyemalis) are socially monogamous, but they engage in extra‐pair copulations (EPCs). We examined spatial activity and behavior of female juncos during their fertile period to determine whether they engaged in tactics likely to facilitate EPCs and whether any such tactics varied with the attractiveness of their social mates. We manipulated the attractiveness of social mates by implanting experimental males with tubes containing testosterone (T‐males) and control males with empty tubes (C‐males). Previous findings in free‐living juncos showed that females mated to C‐males were more likely to produce extra‐pair young than females mated to T‐males. We radio‐tracked 13 females (eight C‐mated, five T‐mated) for an average of 15 h each over 3 d during their fertile periods. We predicted that C‐mated females, to compensate for the induced relative unattractiveness of their social mates, would foray from their territories to seek EPCs and as a result would have larger home ranges than T‐mated females. Females of both treatment groups made extra‐territorial forays, some of considerable distances, but we observed no EPCs during forays. Further, neighboring T‐ and C‐males frequently made incursions into the home ranges of T‐ and C‐mated females but we saw no EPCs during these incursions. Our ability to detect statistical differences was limited by sample size, but given that constraint, we found no detectable difference in female home‐range size in relation to the treatment of their mates, nor did other female behavior differ according to male treatment. Male behavior was significantly affected by testosterone treatment. C‐males guarded their mates more closely than did T‐males. We conclude that female juncos make extra‐territorial movements during their fertile period without regard to male attractiveness (testosterone treatment), but we found no evidence that these function as a special tactic to gain EPCs.     

Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: The Biology of Female and Male Sterility

High levels of female and male sterility were observed among the hybrids from one of the two reciprocal crosses between a wild strain of D. melanogaster known as pi2 and laboratory strains. The sterility, which is part of a common syndrome called hybrid dysgenesis, was found to be associated with the rudimentary condition of one or both of the ovaries or testes. All other tissues, including those of the reproductive system were normal, as were longevity and mating behavior. The morphological details of the sterility closely mimic the agametic condition occurring when germ cells are destroyed by irradiation or by the maternal-effect mutation, grandchildless. We suggest that sterility in hybrid dysgenesis is also caused by failure in the early development of germ cells. There is a thermo-sensitive period beginning at approximately the time of initiation of mitosis among primordial germ cells a few hours before the egg hatches and ending during the early larval stages. Our results suggest that hybrid dysgenesis, which also includes male recombination, mutation and other traits, may be limited to the germ line, and that each of the primordial germ cells develops, or fails to develop, independently of the others. This hypothesis is consistent with the observed frequencies of unilateral and bilateral sterility, with the shape of the thermosensitivity curves and with the fact that males are less often sterile than females. The features of this intraspecific hybrid sterility are found to resemble those seen in some interspecific Drosophila hybrids, especially those from the cross D. melanogaster X D. simulans.     

No Evidence for Significant Effect of Body Size and Age on Male Mating Success in the Spot-legged Treefrog

In anurans, body size and age of individuals generally affect male mating success. To test whether body size and age have effects on male mating success in the foam-nesting treefrog Polypedates megacephalus, a species widely distributed in China, we analyzed differences in body size and age between mated and unmated males for three populations using a Generalized Linear Mixed Model(GLMM). The results showed that mated males did not exhibit larger body size and older age than unmated males, suggesting that large and/or old male individuals did not have greater mating success than small and/or young males. Moreover, we also found a non-significant size-assortative mating pattern for all populations. Our findings suggest that body size and age of the foam-nesting treefrog do not affect male mating success.     

Proteomic Changes between Male and Female Worms of the Polychaetous Annelid Neanthes arenaceodentata before and after Spawning

The Neanthesacuminata species complex (Polychaeta) are cosmopolitan in distribution. Neanthesarenaceodentata, Southern California member of the N. acuminata complex, has been widely used as toxicological test animal in the marine environment. Method of reproduction is unique in this polychaete complex. Same sexes fight and opposite sexes lie side by side until egg laying. Females lose about 75% of their weight and die after laying eggs. The male, capable of reproducing up to nine times, fertilizes the eggs and incubates the embryos for 3-4 weeks. The objective of this study was to determine if there is any set of proteins that influences this unique pattern of reproduction. Gel-based two-dimensional electrophoresis (2-DE) and gel-free quantitative proteomics methods were used to identify differential protein expression patterns before and after spawning in both male and female N. arenaceodentata. Males showed a higher degree of similarity in protein expression patterns but females showed large changes in phosphoproteme before and after spawning. There was a decrease (about 70%) in the number of detected phosphoproteins in spent females. The proteins involved in muscular development, cell signaling, structure and integrity, and translation were differentially expressed. This study provides proteomic insights of the male and female worms that may serve as a foundation for better understanding of unusual reproductive patterns in polychaete worms.     

No Linkage between Genes Controlling Female Pheromone Production and Male Pheromone Response in the European Corn Borer, Ostrinia Nubilalis Hubner (Lepidoptera; Pyralidae)

The E and Z pheromonal strains of the European corn borer, Ostrinia nubilalis, are characterized by female production of and male preference for opposite blends of (E)-11-and (Z)-11-tetradecenyl acetate. It is known that the pheromone production is controlled by an autosomal gene and that the males' behavior is determined by a sex-linked gene. A third gene, autosomally inherited, has been shown to determine the organization of the male pheromone receptors. In the present study the linkage relationship between the autosomal genes controlling sex pheromone production and male olfactory sensilla was investigated. A recombination experiment showed unequivocally that the genes determining the variation in pheromone production and male pheromone receptors are not closely linked and are most likely inherited independently.     

The Subadult Plumage of Male Purple Martins: Variability,Female Mimicry and Recent Evolution

Like a number of other passerine birds year-old male purple martins, Progne subis, often breed in a ♀-like plumage. We have examined delayed plumage maturation in martins by testing assumptions and predictions derived from two hypotheses advanced to explain their appearance and behavior: the sexual selection hypothesis and the female mimicry hypothesis. First, we examine the success of subadults of different appearance 1) in dominance interactions with adults and with other subadults and 2) at entering colonies with different numbers of adults. Second, we compare the mating success of subadults of different appearance. Finally, we examine predictions from the female mimicry hypothesis concerning the appearance of subadults with regard to geographic variation in colony size and with regard to historical changes in colony size in Eastern North America.     

Age-Group-Specific Associations between the Severity of Obstructive Sleep Apnea and Relevant Risk Factors in Male and Female Patients

Aim

To seek accurate and credible correlation manner between gender, age, and obesity; and the severity of obstructive sleep apnea (OSA) in large-scale population.

Methods

Totals of 1,975 male and 378 female OSA patients were sequentially recruited. Centralized covariant tendencies between age, body mass index (BMI), and waist hip ratio (WHR); and OSA severity, were explored in a gender-specific manner via multiple statistical analyses. The accuracies of observed correlations were further evaluated by adaptive multiple linear regression.

Results

All of age, BMI, WHR, smoking, drinking, and OSA severity differed between males and females. BMI and WHR were positively and (approximately) linearly associated with OSA severity in both males and females. Restricted cubic spline analysis was more effective than was the Pearson correlation approach in correlating age with AHI, and provided age crossover points allowing further piecewise linear modeling for both males and females. Multiple linear regression showed that increasing age was associated with OSA exacerbation in males aged ≤40 years and in females aged 45–53 years. BMI, WHR, and diabetes were independently associated with OSA severity in males with age-group-specific pattern. In females, only BMI was associated with OSA severity at all ages.

Conclusions

In male patients, BMI and WHR are prominent risk factors for OSA exacerbation. Age and diabetes are associated with OSA severity in males of particular ages. In females, BMI is also a prominent risk factor for severe OSA, and OSA severity increased with age in the range 45–53 years.