The anostracan genus Branchinella (Crustacea: Branchiopoda), in need of a taxonomic revision; evidence from penile morphology

Three morphologically different penile types discovered in branchinellids from Botswana are compared with literature information on congeners. The striking differences among penile structures in Branchinella ondonguae, B. ornata and the halobiontic B. spinosa raise doubts about their congeneric status. Penis morphology in B. ondonguae corresponds with the most common configuration in branchinellids, but it is largely deviant in the other two. Branchinella ornata shares penile structures with Dendrocephalus , while basal parts in B. spinosa reflect affinity with chirocephalids. A considerable general morphological variability in Branchinella , not met in any other anostracan genus, may reflect the antiquity of the group, or else poor taxonomy. Large intra-branchinellid variability and vague generic boundaries, even when considering the usually conservative penile structures, stress the need of a thorough revision of the entire family of the Thamnocephalidae.     

Vergleichende Ultrastrukturuntersuchungen der Eu- und Paraspermien von 13Protodrilus-Arten (Polychaeta,Annelida) und ihre taxonomische und phylogenetische Bedeutung

The morphology of the slender, filiform spermatozoa of 13Protodrilus species of 22 different populations is investigated by light and transmission electron microscopy. All species have two types of spermatoza: fertile euspermatozoa, and paraspermatozoa, which are probably infertile and may comprise up to 20% of the total number of mature gametes. This is the first record of sperm dimorphism in polychaetes. The general construction pattern of the euspermatozoa is very complex. It shows a longish tapering acrosomal vesicle with an internal acrosomal rod, a rod-like conical nucleus, and a midpiece with numerous very complex supporting elements and two thin mitochondrial derivatives. Further, it has a ‘peribasal body’ surrounding the basal body of the axoneme, an anulus region with an ‘anchoring apparatus’ and an anulus cuff. Posteriorly, the tail region proper contains in some species 2 to 9 supporting rods. In several species the euspermatozoon shows very distinct and species-specific alternations of this ‘general pattern’ relating to e.g. size of sperm elements, structure of acrosome and nucleus, presence or absence of axial rod, and number, shape and size of supporting elements in midpiece and tail. In a number of species some sections of the euspermatozoon overlap with each other more or less strongly. The paraspermatozoon has a comparatively simple construction pattern and possesses no supporting structures in midpiece and tail region. The midpiece is very short and, in some species, entirely surrounded by its two thin and elongate mitochondrial derivatives. An axial rod is often missing or reduced; different sperm sections never overlap each other. In contrast to the euspermatozoa, the paraspermatozoa of the different species have a very similar ultrastructure. Their possible function in spermatophore transfer and histolytical opening of the female epidermis is discussed. A comparison of the different forms of euspermatozoa inProtodrilus elucidates possible plesiomorphous and apomorphous sperm traits. Very likely, the hypothetical plesiomorphous type of spermatozoa inProtodrilus has a very similar morphology to that of the paraspermatozoa, which for this reason are considered to be a sort of persisting representatives of the ancientProtodrilus sperm type. InProtodrilus, the different traits of the euspermatozoa represent excellent taxonomic characters for distinguishing species (e.g. ‘sibling species’). They can also be used well for phylogenetics within the genus, whereas the relations ofProtodrilus to other polychaete groups cannot be clarified solely on the basis of sperm characters, since in all groups the sperm structure is primarily an adaptation to a specific mode of reproduction. Generally, the value of sperm characters in phylogenetic considerations at higher taxonomic levels seems to be very limited due to the surprisingly wide range of different sperm structures within a single genus as is demonstrated in the present paper.      

Sexual selection,genital morphology,and copulatory behavior in male Galagos

Variations in penile morphology among galago species are pronounced and complex. Comparative studies of galagos and other primate species show that elongation of the baculum (os penis)is associated with copulatory patterns involving a prolonged period of intromission. The enlarged penile “spines” of male galagos may be important in maintaining a genital “lock” during copulation. In primate species where females mate with a number of partners, sexual selection may have favored the rapid evolution of such features of penile morphology and masculine copulatory behavior. It is suggested that evolution of complex penile morphologies in galagos has been influenced by sexual selection and that such morphological variations are extremely useful in taxonomic studies.     

Euroceros bulgaricus gen. nov., sp. nov. from Hadzhidimovo (SW Bulgaria) (Late Miocene) - the first European record of Hornbills (Aves: Coraciiformes)

A new genus and species of ground-hornbill are described from the Late Tertiary (Late Miocene) fluviative deposits in Hadzhidimovo (Hadzhidimovo-1, or Hadzhidimovo-Girizite locality), in southwestern Bulgaria. The cranial half of a scapula and the distal third of a femur have been recovered. The morphology distinguishes the specimen from the recent species of Bucorvus, the closest genus. Most interesting is the morphology of the scapula (the holotype), which differs considerably from the ground-hornbills in some structures (e.g. the clavicular joint) while being surprisingly similar in other structures (e.g. the humeral articular joint). The last peculiarity may indicate that the fossil species was a more primitive form having relatively poorer flying capacity in comparison to other ground-hornbills. This first record of Bucerotidae in Europe proves the occurrence of the family in the Tertiary of the western parts of the Eurasian continent. It came from the richest Bulgarian locality of the Hipparion fauna, from which ca. 36 species of large mammals and birds are now known.     

中国金粉蕨属孢子形态的研究

利用扫描电子显微镜对我国产金粉蕨属8种2变种蕨类植物的孢子进行了观察。结果表明,该属孢子形态较为一致。三裂缝,辐射对称,极面观为钝三角形或三角圆形,赤道面观为椭圆形,超半圆形成或扇形,周壁较薄,外壁突起形成表面纹饰的基本轮廓,具赤道环,近极脊和远极脊等结构,种间差异主要表现在远极面的纹饰形态上,从孢子形态上看,金粉蕨属与中国蕨科其它属差异较大,而与凤尾蕨属相似,建议将本属放在凤尾蕨科。     

Classical and dynamic morphology: toward a synthesis through the space of forms

In plant morphology, most structures of vascular plants can easily be assigned to pre-established organ categories. However, there are also intermediate structures that do not fit those categories associated with a classical approach to morphology. To integrate the diversity of forms in the same general framework, we constructed a theoretical morphospace based on a variety of modalities where it is possible to calculate the morphological distance between plant organs. This paper gives emphasis on shoot, leaf, leaflet and trichomes while ignoring the root. This will allow us to test the hypothesis that classical morphology (typology) and dynamic morphology occupy the same theoretical morphospace and the relationship between the two approaches remains a question of weighting of criteria. Our approach considers the shoot (i.e. leafy stem) as the basic morphological structural unit. A theoretical data table consisting of as many lines as there are possible combinations between different modalities of characters of a typical shoot was generated. By applying a principal components analysis (PCA) to these data it is possible to define a theoretical morphospace of shoots. Typical morphological elements (shoots, leaves, trichomes) and atypical structures (phylloclades, cladodes) including particular cases representing ‘exotic’ structures such as the epiphyllous appendages of Begonia and ‘water shoot’ and ‘leaf’ of aquatic Utricularia were placed in the morphospace. The more an organ differs from a typical shoot, the further away it will be from the barycentre of shoots. By giving a higher weight to variables used in classical typology, the different organ categories appear to be separate, as expected. If we do not make any particular arbitrary choice in terms of character weighting, as it is the case in the context of dynamic morphology, the clear separation between organs is replaced by a continuum. Contrary to typical structures, “intermediate” structures are only compatible with a dynamic morphology approach whether they are placed in the morphospace based on a ponderation compatible with typology or dynamic morphology. The difference in points of view between typology and continuum leads to a particular mode of weighting. By using an equal weighting of characters, contradictions due to the ponderation of characters are avoided, and the morphological concepts of continuum’ and ‘typology’ appear as sub-classes of ‘process’ or ‘dynamic morphology’.     

Effects of exogenous B supply on growth, B accumulation and distribution of two navel orange cultivars

To investigate the effects of boron (B) on growth, B concentration and distribution of two navel orange cultivars, ‘Newhall’ (Citrus sinensis Osbeck) and ‘Skagg’s Bonanza’ (Citrus sinensis Osbeck) grafted on the rootstock trifoliate orange [Poncirus trifoliata (L.) Raf.], B at five levels was exogenously supplied to 1-year-old grafted plants of both cultivars under greenhouse conditions. Plants were grown in sand:perlite (1:1, v/v) medium and were irrigated every 2 days with half-strength Hoagland’s No. 2 nutrient solutions containing different B, 0.01, 0.05, 0.10, 0.25 and 2.50 mg l⁻¹ (0.25 and 2.50 mg l⁻¹ were considered as control and excess B treatment, respectively, and the other three B levels were considered as low B treatments). After treatments for 183 days, leaves (from basal, middle, upper parts of the shoots), stem of scion, stem of rootstock and root were separately sampled. Our results showed that plant growth (plant height, root volume and dry weights of various parts) was inhibited in response to low or excess B supplies in both cultivars. It was found that B concentrations in the upper leaves of both cultivars were substantially higher than those in the basal leaves when low concentrations (≤0.05 mg l⁻¹) of exogenous B were applied, suggesting that B was preferentially translocated to the upper-younger leaves to support their growth. Analysis of B distribution in different parts indicated that translocation of B from the root to the scion’s shoots (stems and leaves of scion) may be restricted upon exposure to low B conditions. When B was inadequately supplied, growth of ‘Skagg’s Bonanza’ was better than ‘Newhall’, implying that the former cultivar was more tolerant to low B status, which may be due to the higher efficiency of B translocation from the root to the scion’s shoots. However, when the plants were treated with excess B (2.50 mg l⁻¹), both cultivars showed a similar degree of B toxicity. The probability of scion–rootstock interactions in relation to the differential responses of growth and different efficiency of B translocation involved in the two orange cultivars following the long-term low B stress were discussed.     

The structure of rice roots grown in aerobic and anaerobic environments

Summary Anatomy and structure was examined in roots of rice grown in aerated solutions for 4 weeks (‘non-adapted plants’) or for the last 6 days in N₂-flushed solutions (‘adapted plants’). Structures of roots of adapted and non-adapted plants were similar. In both cases, the cortex of basal tissue consisted predominantly of elongated cells which extended radially across almost the entire cortex. A lack of protoplasm in many of these cells indicates that they have no metabolic function, and longitudinal sections show that these structures may constitute a pathway for oxygen movement down the root. Apical tissues did not contain this type of structure. re]19760217     

Molecular phylogenetics of the genus <Emphasis Type="Italic">Physalia</Emphasis> (Cnidaria: Siphonophora) in New Zealand coastal waters reveals cryptic diversity

Physalia is a genus of pelagic colonial hydrozoans often known by common names such as ‘Portuguese-man-of-war’ or ‘bluebottle’. Siphonophore systematists generally recognise only a single species in this genus, Physalia physalis, however the name Physalia utriculus is also still in common use, which has led to considerable taxonomic confusion. With some morphological variation between global regions there is the possibility that this genus holds a substantial amount of cryptic variation. We seek to examine the genetic structure of Physalia present in New Zealand coastal waters. Fifty-four specimens collected from 13 locations around New Zealand and Australia were sequenced for both mitochondrial cytochrome c oxidase I (COI) and the first internal transcribed spacer (ITS1) of the nuclear ribosomal cistron. Sequences were analysed using maximum likelihood and split decomposition neighbour networks to determine conflict between clans (the unrooted analog of clades). Three clans were identified from both the COI and ITS sequences. The results are complex and clans are not consistent between the two genes. Nevertheless, it seems that there is substantial cryptic diversity amongst Physalia present in New Zealand coastal waters.     

Uncovering the Laurasian roots ofEubranchipus

The species of the anostracan genusEubranchipus Verrill, 1870 can be recognized as belonging to the same taxon and unambiguously distinguished from all other taxa in the family Chirocephalidae Daday, 1910 by the common morphology of their eversible penes. These reproductive structures are short, fat, and cylindrical with a single long, thin spine on the lateral margin at the tip of each penis. This ends the erroneous perception ofEubranchipus as a New World genus and recognizes it as an old taxon with members distributed accross the modern continents that once comprised Laurasia.     

Pollen morphology and infra-generic evolutionary relationships in some Chinese species of Pedicularis (Scrophulariaceae)

 The pollen grains of 32 Chinese species of Pedicularis representing 13 groups within the genus were investigated using light microscopy and scanning electron microscopy. The genus is eurypalynous, the apertures may be tricolpate, trisyncolpate, or bisyncolpate and five types of surface ornamentation (microscabrate, microrugulate, retipilate, microreticulate and microfoveolate) were observed. Comparison of the different aperture types using light microscopy allows three pollen types to be distinguished. Examination of exine ornamentation with scanning electron microscopy enabled each pollen type to be divided into two or three sub-types (giving a total of seven sub-types). Pollen morphology within the genus is discussed in relation to infrageneric relationships, evolutionary and pollination biology. There is little correlation with the existing infrageneric taxonomy (some taxonomic groups have more than one pollen type, while the same pollen type may be found in several different species-groups), but greater correlation with the corolla morphology. Received January 10, 2002; accepted March 5, 2002 Published online: March 10, 2003     

The enigmatic Alpine opilionid Saccarella schilleri gen. n., sp. n. (Arachnida: Nemastomatidae)—isolated systematic placement inferred from comparative genital morphology

In Opiliones, male genital morphology plays a key role in systematics and understanding the evolution of the group because functional modifications often outline higher taxonomic levels. In Nemastomatidae, different lateral stabilizing structures on the penial truncus cane developed independently. These wing-like protuberances serve as anchor points for two muscular tendons and are interpreted traditionally as generic characters. A newly discovered species from Monte Saccarello in the Ligurian Alps of North-western Italy is unique in providing anchor points as stabilizing parts of the distal truncus cane below the glans by short and narrow lateral folds. To place this unusual species within the Nemastomatidae one must re-evaluate the usefulness of male genital morphological and other characters used in the systematics of the family. The resulting phylogenetic framework is supplemented with available molecular genetic data. Based on both datasets, the creation of a new genus seems justified. Saccarella schilleri gen. n. sp. n. is subsequently described and further defined. The endemism and biogeography of the harvestmen fauna in the South-western Alps are discussed with respect to this enigmatic genus.     

The Philodinavidae (Rotifera Bdelloidea): a special family

Here we focus on the fine morphology and present observations on the biology of representatives of family Philodinavidae. Philodinavus paradoxus and Henoceros falcatus were collected and cultured under laboratory conditions. Rotifers of both species are tiny, about 200 μm long, have protrudable trophi and creep with leech-like movements. A very specific feature of these rotifers is their corona; a V-shaped lower lip contours the mouth opening, bilaterally bordered by two arched cuticular structures (‘cheeks’). The presence of the cheeks is a feature shared by the third genus, Abrochtha. On the basis of the morphology and biology of the three genera, we advance the hypothesis that Philodinavus is a primitive bdelloid, and that it can have originated Henoceros and Abrochtha, from which the other bdelloids could have stemmed. This revised version was published online in September 2006 with corrections to the Cover Date.     

Genetic and Electron-Microscopic Characterization of ‘<Emphasis Type="Italic">Rickettsiella agriotidis</Emphasis>’, a new <Emphasis Type="Italic">Rickettsiella</Emphasis> Pathotype Associated with Wireworm, <Emphasis Type="Italic">Agriotes</Emphasis> sp. (Coleoptera: Elateridae)

Wireworms, the polyphagous larvae of click beetles belonging to the genus Agriotes (Coleoptera: Elateridae), are severe and widespread agricultural pests affecting numerous crops. A previously unknown intracellular bacterium has been identified in a diseased Agriotes larva. Microscopic studies revealed the subcellular structures characteristic of Rickettsiella infections. Molecular phylogenetic analysis based on 16S ribosomal RNA and signal recognition particle receptor (FtsY) encoding sequences demonstrates that the wireworm pathogen belongs to the taxonomic genus Rickettsiella. Therefore, the new pathotype designation ‘R. agriotidis’ is proposed to refer to this organism. Moreover, genetic analysis makes it likely that—on the basis of the currently accepted organization of the genus Rickettsiella—this new pathotype should be considered a synonym of the nomenclatural type species, Rickettsiella popilliae.     

Multiple lines of evidence for an Australasian geographic boundary in the Indo-Pacific humpback dolphin (<Emphasis Type="Italic">Sousa chinensis</Emphasis>): population or species divergence?

The taxonomic status of humpback dolphins (genus Sousa, sub-family Delphininae) is unresolved. While the classification of this genus ranges from a single to three nominal species, the International Union for Conservation of Nature and the International Whaling Commission only recognise a ‘two-species’ taxonomy (S. teuszii in west Africa, and S. chinensis in the Indo-Pacific). Under the IUCN (2008), S. chinensis is listed as ‘near threatened’, but is only considered as a ‘migratory’ species in Australia. Taxonomic resolution of the genus Sousa is needed to define particular conservation status and develop appropriate management actions. Using phylogenetic analyses of 1,082 bp of mitochondrial and 1,916 bp of nuclear DNA, we provide multiple lines of genetic evidence for the genetic distinction of S. chinensis in China and Indonesia from S. chinensis in Australia. The separation of Australian Sousa from Sousa of Southeast Asia requires a review of their current conservation status and respective management actions.     

The female reproductive organ in podocopid ostracods is homologous to five appendages: histological evidence from Liocypris grandis (Crustacea,Ostracoda)

The giant ostracod, Liocypris grandis, found in South African temporary pools, has five pairs of additional appendage-like structures, seemingly associated with the Female Reproductive Organ (FRO). Original investigations of these structures by light and scanning electron microscopy could not unequivocally determine whether or not these structures are homologous to genuine appendages and if so, to how many. The present paper investigates morphology and attachment of these structures by frontal and transversal micro-sectioning (thickness = 1 μ m) of the caudal soft parts of mature females of L. grandis. It is found that all five appendages (R1–R5) have one individual attachment point where they enter into the FRO. Also R3(I) and R3(II) have a single attachment only, these two parts thus represent one single appendage. Whereas R1–R4 have clear lateral insertions, R5 is caudally positioned. The fusion of this latter appendage with the FRO occurs internally, at the posterior edge of the bulge of the FRO and is not externally visible. It is herewith thus accepted that R1–R5 are genuinely endogenous structures (and not, for example, exogenous parasites), that the five structures are homologous to five appendages, and not for example to parts of biramous appendages. It follows from this that the female reproductive organ is homologous to at least five, not to one segment. This would bring the number of (original) thoracic segments in podocopid ostracods to at least 8. An additional pair of triangular structures, inserted in between the two FRO is here described as structures ‘X’. Their homology and function remain unknown.     

Morphology and evolution of the tarsal plantulae in Hymenoptera (Insecta), focussing on the basal lineages

The morphology of the plantulae (= tarsal pulvilli = plantar lobes), structures attached to the underside of the tarsus in Hymenoptera, was examined in 55 genera from all 14 families of the basal lineages of Hymenoptera ('Symphyta') and a few species of Apocrita, using scanning electron microscopy. Two distinct types of plantula were found: (1) integrated , an unsclerotized patch positioned ventro-distally on each tarsomere, and (2) distal , a membranous vesicle attached to the apical end of each tarsomere. The evolution of these two types is discussed in the light of current phylogenetic hypotheses. The plantulae exhibit an astonishing variety of form and structural details; their potential phylogenetic and taxonomic value is discussed.     

Generic and species concepts in Microglena (previously the Chlamydomonas monadina group) revised using an integrative approach

Traditionally the genus Microglena Ehrenberg has been used to contain species that belong to the Chrysophyceae; however, the type species of Microglena, M. monadina, represents a green alga, which was later transferred to the genus Chlamydomonas. The taxonomic status of the genus has therefore remained unclear. We investigated 15 strains previously assigned to C. monadina and two marine species (C. reginae and C. uva-maris) using an integrative approach. Phylogenetic analyses of SSU and ITS rDNA sequences revealed that all strains form a monophyletic lineage within the Chlorophyceae containing species from different habitats. The strains studied showed similar morphology with respect to cell shape and size, but showed differences in chloroplast and pyrenoid structures. Some representatives of this group have the same type of sexual reproduction (homothallic advanced anisogamy). Three different morphotypes could be recognized. Strains belonging to type I have a cup-shaped chloroplast with a massive basal part, in which a large, single, ellipsoidal pyrenoid is located. The members of type II also have a cup-shaped chloroplast, which is partly lobed and has a thinner basal part than type I; here the pyrenoid is half-ring or horseshoe-shaped and occupies different positions in the chloroplast depending on the strain. The strains of type III have multiple pyrenoids, which appear to have developed from the subdivision of a single ring-shaped pyrenoid into several parts. We compared the results of our morphological investigations with the literature and found that 15 strains could be identified with existing species. Two strains did not fit with any described species. As a result of our study, we transfer all strains to the genus Microglena, propose 11 new combinations, and describe two new species. Comparison of the ITS-1 and ITS-2 secondary structures confirmed the species delineations. All species have characteristic compensatory base changes in their ITS secondary structures and are supported by ITS-2 DNA barcodes.     

Microfacies controls on weathering of carbonate building stones: Devonian (northern Sauerland,Germany)

Summary The Beisinghausen Limestone (Upper Givetian to Frasnian) in the Eslohe-Reiste area (northern Sauerland), used in the past as building stone, corresponds to proximal carbonate turbidites which have been derived from the Attendom-Elspe ‘reef’ complex. The particles of this allodapic limestone originated in different parts of the carbonate complex as shown by facies-diagnostic microfossils (foraminifera, calcisphaeres, calcareous algae, microproblematica). The fossils as well as the other dominating grain types (lumps, peloids) point to source areas located within lagoonal and slope environments. Reef-derived material is rare. The turbidites exhibit four microfacies types, differentiated by composition and size of the grains as well as by micrite content and corresponding to the common vertical and lateral textural variation of limestone turbidites. These MF types were recognized in outcrops as well as from building stones used in building the St. Pankratius church in Eslohe-Reiste (northern Sauerland) in 1849 and in the renovation of the church in 1963/64. The comparison of microfacies and the degree of the destruction of ‘old’ and ‘new” building stones by weathering (macroscopically described by the ◂Fabric Index’: Product of the ‘Rock Destruction Risk’ and the ‘Rock Preparation Destruction Degree’) shows that intrabioclastic rudstones (MF type 1) and bioclastic grainstones (MF type 2), both characterizing the basal parts of the turbidite beds, are more resistant to weathering destruction originating from freezing and thawing than packstones (MF type 4). Weathering of micritic facies types (e.g., MF 4) is more intensive due to the stronger development of joint systems affecting not only the surface of the building stones but the entire dimension stone. Porosity or the existence and amount of stylolites seem to have had no significant impact on the weathering of the building stones studied. The stronger weathering of building stones used in the original construction of the church as compared with the stones applied in this century is caused by the greater time interval available for mechanical weathering connected with freezing and thawing. The consideration of microfacies of limestone turbidites should facilitate the exploitation of weathering-resistant carbonate building stones.