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1.
Twenty-two polymorphic microsatellites were isolated from the noble scallop, Chlamys nobilis with a partial genomic library enriched for tandem repeat sequences of (CA)12 and (GA)12. Polymorphism of these loci was assessed in a sample of 32 unrelated individuals. The average allele number was 7.23 per
locus with a range of 3–19, and the observed and expected heterozygosity varied from 0.032–0.875 (averaging 0.452) and 0.315–0.939
(averaging 0.646), respectively. After Bonferroni correction (P > 0.0023), 11 of the 22 markers accorded with Hardy–Weinberg equilibrium, and the rest showed significant departure from HWE.
Three pairs of loci showed significant linkage disequilibrium (P < 0.01). A poor transferability of these loci to other two cultured species, C. farreri and Patinopecten yessoensis was detected. These markers would be useful for studies of population genetics, linkage mapping and other relevant research
in C. nobilis. 相似文献
2.
Wouter F. D. van Dongen Gopi K. Munimanda Jakob Augustin Donald Blomqvist Tibor Szép Richard H. Wagner 《Journal of Ornithology》2010,151(3):761-764
We isolated and characterised six novel microsatellite loci for paternity analysis in the sand martin Riparia riparia, by screening an enriched genomic library. In addition, we tested 16 already published microsatellite markers, five of which
were also polymorphic in the sand martin. Only one of these 11 loci exhibited evidence of null alleles, and all were polymorphic
(mean H
o = 0.68, range of number of alleles per locus = 4–24), making them suitable for individual heterozygosity quantification and
paternity assessment in this species (exclusion probability of 11 unlinked loci = 0.999997). 相似文献
3.
Claudete F. Ruas Thiago J. Nakayama M. Á. Ortiz Mayra A. Kuroki Tod F. Stuessy Karin Tremetsberger Eduardo A. Ruas Melissa de Oliveira Santos Salvador Talavera Paulo M. Ruas 《Conservation Genetics》2009,10(5):1413-1416
We report the isolation and characterization of eight microsatellite loci for Hypochaeris salzmanniana, an endangered species endemic to the southwestern coast of Spain and the Atlantic coast of Morocco. A total of 32 alleles
were detected across a sample of 45 individuals, with an average number of 4.0 alleles per locus. The average polymorphic
information content (PIC) was 0.533 and the observed (H
O) and expected (H
E) heterozygosity values varied from 0.022 to 0.978 and from 0.434 to 0.759, respectively. Five loci exhibited significant
deviation from Hardy–Weinberg equilibrium (P ≤ 0.001) and three pairs of loci showed significant linkage disequilibrium (P ≤ 0.01). The eight loci were tested for transferability in three others species (H. arachnoidea, H. glabra, and H. radicata) belonging to the same section of H. salzmanniana. With the exception of locus Hsalz7, all loci successfully amplified in the three species. These preliminary data confirm
the usefulness of microsatellite markers for assessing the ecology and genetic structure of H. salzmanniana and to understand the evolution of species within the section Hypochaeris. 相似文献
4.
Shi-Chao Xing Gen-Bo Xu Xiao-Lin Liao Guan-Pin Yang Song-Lin Chen 《Conservation Genetics》2009,10(4):1167-1169
In the study, 34 microsatellite loci were isolated from a dinucleotide-enriched genomic library of Japanese Spanish mackerel
(Scomberomorus niphonius). And 12 microsatellite loci were found to be polymorphic between 3 and 8 alleles. The number of observed and expected heterozygosity
per locus in 23 individuals ranged from 0.6087 to 1.0000 and 0.8908 to 0.9773, respectively. Two loci significantly deviated
from Hardy–Weinberg equilibrium after Bonferroni correction analysis and there was no significant linkage disequilibrium found
between pairs of loci. As a result, 12 microsatellite loci probably located on different chromosome pairs and these polymorphic
microsatellite loci should provide sufficient level of genetic diversity to investigate the fine-scale population structure
and evaluate the breeding strategy in S. niphonius.
The authors Shi-Chao Xing and Gen-Bo Xu contributed equally. 相似文献
5.
Guidong Miao Changwei Shao Hongyu Ma Xiaolin Liao Songlin Chen 《Conservation Genetics》2009,10(3):631-633
Starry flounder (Platichthys stellatus) is a rare fish species in China. Here, we reported 12 polymorphic microsatellite loci isolated from a dinucleotide-enriched
genomic library of starry flounder (P. stellatus). The number of alleles, observed and expected heterozygosity per locus in 30 individuals ranged from two to six, from 0.2500
to 1.0000 and from 0.4512 to 0.7667, respectively. One locus significantly deviated from Hardy–Weinberg equilibrium after
Bonferroni correction and no significant linkage disequilibrium between pairs of loci was found. Cross-species amplification
of these microsatellite loci in additional three fish species was performed. These polymorphic microsatellite loci would be
useful for investigating genetic population structure and construction of genetic linkage map in P. stellatus.
Guidong Miao and Changwei Shao have contributed equally. 相似文献
6.
Jin-Zhen Bi Chang-Wei Shao Gui-Dong Miao Hong-Yu Ma Song-Lin Chen 《Conservation Genetics》2009,10(4):1171-1173
The cutlassfish (Trichiurus haumela) is an important commercial fish species. In the present study, we report 12 polymorphic microsatellite DNA markers for cutlassfish.
The number of alleles per locus ranged from 2 to 9 in a sample of 26 individuals. Observed and expected heterozygosities per
locus varied from 0.2727 to 0.9583 and from 0.4059 to 0.7926, respectively. Two loci (Trha25 and Trha27) showed significant
departure from Hardy–Weinberg equilibrium after sequential Bonferroni correction (P < 0.0042). No significant linkage disequilibrium between pairs of loci was found. These microsatellite markers provide powerful
tools for investigating genetic population structure, population history and conservation management of cutlassfish.
Jin-Zhen Bi and Chang-Wei Shao contributed equally to this work. 相似文献
7.
X-chromosomal short tandem repeats (X-STR) loci are used for forensic practice in recent years in some complex kinship cases.
The commercially available kit of Investigator Argus X-12 (Qiagen, Hilden, Germany) makes it possible to examine the markers
of DXS10148–DXS10135–DXS8378, DXS7132–DXS10079–DXS10074, DXS10103–HPRTB–DXS10101 and DXS10146–DXS10134–DXS7423, which belong
to four linkage groups of X-chromosome. In this study, a total of 309 unrelated individuals (200 males and 109 females) from
Shanghai Han population were successfully analyzed with this kit. Hardy–Weinberg equilibrium tests demonstrated no significant
deviation from expected values (P > 0.05) for all of the 12 X-STR loci in the Shanghai Han population. Linkage disequilibrium tests were performed for all
pairs of loci by the Arlequin v3.1 software and only DXS10103–DXS10101 remained significant after adjustment for multiple
testing (P < 0.05/66). The combined power of discrimination in males (CDPM) was 0.999999996 while in females (CDPF) was 0.999999999999995, and the combined mean exclusion chance in duo cases (CMECD) was 0.999998 while in trio cases (CMECT) was 0.999999986. The results suggest that the twelve X-STR loci may provide high polymorphic information for paternity testing
and forensic identification in Chinese Han population from Shanghai. 相似文献
8.
By using a modified biotin–streptavidin capture method, a total of 12 microsatellite loci were developed and characterized
for Michelia coriacea (Magnoliaceae), a critically endangered endemic to Southeast Yunnan, China. The number of alleles (A) ranged from two to six in 30 samples of this species. The ranges of observed (H
O
) and expected (H
E
) heterozygosities were 0.033–0.8000 and 0.033–0.7910, respectively. Cross-species amplification in M. yunnanensis showed that a subset of these markers holds promise for congeneric species. These polymorphic SSR markers would be useful
tools for population genetics studies on M. coriacea and other congeneric species. 相似文献
9.
We describe the isolation and characterization of ten microsatellite loci from the red-winged tinamou (Rhynchotus rufescens) and also evaluated the cross-amplification of these loci and other ten loci previously developed for the great tinamou (Tinamus major) in other tinamous. Genetic variability was assessed using 24 individuals. Six loci were polymorphic with moderate to high
number of alleles per locus (2–12 alleles) and showed expected heterozygosity (HE) ranging from 0.267 to 0.860. All loci conformed
to the Hardy–Weinberg expectation and linkage disequilibrium was not significant for any pair of loci. This battery of polymorphic
loci showed high paternity exclusion probability (0.986) and low genetic identity probability (4.95 × 10−5), proving to be helpful for parentage tests and population analyses in the red-winged tinamou. The cross-amplification was
moderate where of the 160 locus/taxon combinations, 46 (28.75%) successfully amplified. 相似文献
10.
The mud crab Scylla paramamosain plays a significant role in fishery resources in China. In this study, we developed 18 polymorphic microsatellite markers
in this important crab by 5′ anchored PCR technique. A total of 125 alleles were detected in a single population of 32 individuals
of S. paramamosain. The number of alleles per locus ranged from five to nine, with the allele size ranging from 166 to 316 bp. The polymorphism
information content (PIC), observed and expected heterozygosity ranged from 0.39 to 0.88, from 0.33 to 0.92 and from 0.42
to 0.86, respectively. Three loci (Scypa13, Scypa14 and Scypa15) deviated significantly from Hardy–Weinberg equilibrium (HWE)
after Bonferroni correction (P < 0.0028), and no linkage disequilibrium was found between loci pairs. These polymorphic microsatellite markers will be useful
for the study of population genetic structure, construction of genetic linkage maps and mapping of economically quantitative
trait loci (QTL) in S. paramamosain. 相似文献
11.
The blue mussel Mytilus trossulus occurs in the Pacific and in the North Atlantic. We developed and characterized six microsatellite loci for Baltic M. trossulus. Seventeen microsatellite loci were screened, of which six were polymorphic. The number of alleles among 50 individuals ranged
from 3 to 13 and the observed and expected heterozygosity were 0.09–0.46 and 0.34–0.86, respectively. The loci were also tested
for cross amplification in M. edulis, in which four of the six microsatellite loci were successfully amplified. 相似文献
12.
Woo-Jin Kim Young-Ok Kim Bo-Hye Nam Hee Jeong Kong Eun-Mi Park Jung-Ha Kang Jeong-Ho Lee Kyung-Kil Kim 《Conservation Genetics》2009,10(4):1105-1111
Expressed sequence tags (ESTs) can be used to identify microsatellite markers. We developed 81 polymorphic microsatellite
markers from 4,940 ESTs of the olive flounder, Paralichthys olivaceus. Out of 100 EST-derived microsatellites for which PCR primers were designed, 81 loci were polymorphic in 30 individuals from
a single natural population with 2–28 (mean 10.6) alleles per locus. The observed and expected heterozygosities of these loci
were 0.033–1.000 and 0.033–0.965, respectively. Segregation analysis within a mapping family revealed non-amplifying null
alleles at five loci. These new EST-derived microsatellite markers should be useful for population genetic analyses, pedigree
tracing and constructing a linkage map for olive flounder. 相似文献
13.
Cristaria plicata was an important freshwater mussel for pearl culture in China. 18 polymorphic microsatellite markers were isolated and characterized
using (CA)15-enriched genomic library of C. plicata. These loci showed high levels of genetic polymorphism testing on 60 individuals sampled from Poyang Lake of Jiangxi Province,
China. The number of alleles per locus ranged from 4 to 18. The expected (H
E) and observed heterozygosities (H
O) were 0.7232–0.8961 and 0.0000–1.0000, respectively. Four microsatellite loci were significantly deviated from Hardy–Weinberg
equilibrium and no linkage disequilibrium was found. These microsatellite loci will be useful for assessment of genetic diversity
and population structure in C. plicata. 相似文献
14.
Ardisia crenata, an evergreen shrub native to East Asia, has been a serious invasive plant to the southeastern USA. Here 13 polymorphic microsatellite
loci were isolated and characterized from an enrichment genomic library of A. crenata. The average allele number of these microsatellites was four per locus, ranging from two to seven. The ranges of observed
and expected heterozygosity were 0.000–1.000 and 0.239–0.789, respectively. These microsatellite markers will be useful for
investigating population genetics and reproductive ecology of A. crenata. 相似文献
15.
Xin Zhang Wan-Hui Ye Hong-Lin Cao Zheng-Feng Wang Hao Shen Ju-Yu Lian 《Conservation Genetics》2009,10(6):1833-1835
We developed 11 polymorphic microsatellite primers from Canarium album, which is a famous Chinese fruit tree with diversified economy values. The observed and expected heterozygosities (H
o and H
e) ranged from 0.133 to 0.833 and 0.128 to 0.810, with averages of 0.607 and 0.629, respectively. Four loci were deviated from
Hardy–Weinberg equilibrium after Bonferroni correction (P < 0.01), but no linkage disequilibrium was detected among any pair of loci after Bonferroni correction (P < 0.05). Cross-species amplification in another important cultured species Canarium pimela showed that 10 of 11 primers were polymorphic, with number of alleles per locus ranging from 2 to 5. The primers will be
useful to explore further studies about introgressive hybridization and conversation of wild genetic resources of these two
species. 相似文献
16.
Naofumi Nomura Kento Fujiwara Tokushiro Takaso Motomi Ito Koichi Uehara Hiroaki Setoguchi 《Conservation Genetics》2009,10(4):1093-1095
Eight microsatellite loci for the perennial herb Farfugium japonicum, including the rheophytic variety luchuense endemic to riparian areas of the Ryukyu Islands, Japan, were isolated and characterised. The number of alleles ranged from
5 to 14. The expected (H
E) and observed (H
O) heterozygosities were 0.344–0.885 and 0.121–0.754, respectively, from 69 individuals in one population. Six loci exhibited
significantly fewer heterozygotes than expected under Hardy–Weinberg equilibrium (P < 0.05). The primers amplifying microsatellite sequences in F. japonicum may provide a population genetics tool useful in the establishment of a conservation strategy. 相似文献
17.
In order to study hybridisation, taxonomic boundaries and phylogeography in the genus Pseudopanax (Araliaceae), we developed seven novel microsatellite loci from enriched genomic libraries of P. lessonii and P. crassifolius. These loci were characterised in 16 individuals from a single population of P. crassifolius, and displayed 2–11 alleles per locus. Observed heterozygosities ranged from 0.063 to 0.688. Most loci were polymorphic in
the closely related Pseudopanax species, and several loci amplified widely across the Araliaceae. 相似文献
18.
Soon-Jae Kwon Gi-An Lee Sok-Young Lee Yong-Jin Park Jae-Gyun Gwag Tae-San Kim Kyung-Ho Ma 《Conservation Genetics》2009,10(5):1557-1560
The present study reports isolation and characterization of 21 polymorphic microsatellite markers developed from Lycium chinense Mill. These markers produced a total of 86 alleles across 30 L. chinense accessions, with an average of 4.1 alleles per locus. Values for observed heterozygosity and polymorphism information content
ranged from 0.03 to 0.81 (mean = 0.35) and from 0.03 to 0.78 (mean = 0.31), respectively. At the significance threshold (P < 0.05), 12 loci deviated from Hardy–Weinberg equilibrium, whereas significant linkage disequilibrium values were observed between
52 pairs of loci. All loci were successfully amplified for all L. barbarum accessions. These newly developed polymorphic microsatellite markers will be very useful for programming in the genetic conservation
and classification of L. chinense and L. barbarum. 相似文献
19.
Selvaraju Kanagarajan Saraladevi Muthusamy Emily Chin-Fun Chen Hsin-Sheng Tsay 《Conservation Genetics》2009,10(5):1487-1490
We developed and characterized 8 polymorphic microsatellite loci or simple sequence repeats from the expressed sequence tags
database of the medicinal fungus, Antrodia cinnamomea. Variability was assessed in a panel of 23 strains collected from various regions of Taiwan. The number of alleles per polymorphic
locus ranged from two to seven loci with an average of 4.375 alleles per locus. The observed and expected heterozygosity values
ranged from 0.130 to 0.783 and from 0.122 to 0.809, respectively. No significant Hardy–Weinberg equilibrium was detected (P < 0.01) at all loci. The EST-SSR markers developed in the present study can be used for strain identification and population
genetic studies in A. cinnamomea. 相似文献
20.
Isolation and characterization of microsatellite loci from a potential biofuel plant Miscanthus sinensis (Poaceae) 总被引:1,自引:0,他引:1
Kuo-Hsiang Hung Tzen-Yuh Chiang Chi-Te Chiu Tsai-Wen Hsu Chuan-Wen Ho 《Conservation Genetics》2009,10(5):1377-1380
Miscanthus sinensis (Poaceae) is typical of many dominant grasses of East Asia. Due to its characteristics of fast growth and high biomass, Miscanthus, a C4 plant, has been long explored for the potential usage as biofuel. In this study, we described the development of nine
microsatellite loci from M. sinensis for genetic studies. These new markers were tested in 25 individuals of five populations in three varieties. The number of
alleles ranged from 3 to 8. The expected (H
E) and observed (H
O) heterozygosities were 0.37–0.78 and 0.05–0.52, respectively. All microsatellite loci are significantly deviated from Hardy–Weinberg
expectations likely due to the population structure within samples. Linkage disequilibrium between four loci pairs were observed
from the total 36 pairwise comparisons of loci. Cross-species transferability revealed that all loci can be applied to its
closely related species, M. floridulus. 相似文献