共查询到20条相似文献,搜索用时 15 毫秒
1.
Ramalho PA Paiva S Cavaco-Paulo A Casal M Cardoso MH Ramalho MT 《Applied and environmental microbiology》2005,71(7):3882-3888
Unspecific bacterial reduction of azo dyes is a process widely studied in correlation with the biological treatment of colored wastewaters, but the enzyme system associated with this bacterial capability has never been positively identified. Several ascomycete yeast strains display similar decolorizing behaviors. The yeast-mediated process requires an alternative carbon and energy source and is independent of previous exposure to the dyes. When substrate dyes are polar, their reduction is extracellular, strongly suggesting the involvement of an externally directed plasma membrane redox system. The present work demonstrates that, in Saccharomyces cerevisiae, the ferric reductase system participates in the extracellular reduction of azo dyes. The S. cerevisiae Deltafre1 and Deltafre1 Deltafre2 mutant strains, but not the Deltafre2 strain, showed much-reduced decolorizing capabilities. The FRE1 gene complemented the phenotype of S. cerevisiae Deltafre1 cells, restoring the ability to grow in medium without externally added iron and to decolorize the dye, following a pattern similar to the one observed in the wild-type strain. These results suggest that under the conditions tested, Fre1p is a major component of the azo reductase activity. 相似文献
2.
Reduction and biodegradation mechanisms of naphthylaminesulfonic azo dye amaranth using a newly isolated Shewanella decolorationis strain S12 were investigated. Under anaerobic conditions, amaranth was reduced by strain S12, and a stoichiometric amount
of two reduction products RP-1 and RP-2 were generated. UV/visible spectrophotometric and high performance liquid chromatography
(HPLC) analysis indicated that RP-1 and RP-2 were 1-aminenaphthylene -4-sulfonic acid and 1-aminenaphthylene-2-hydroxy-3,
6-disulfonic acid. The result strongly supports a mechanism of azo dye reduction by the process via the reductive cleavage
of the azo bond to form corresponding aromatic amines. The result of HPLC analyses revealed that these aromatic amines were
not able to be mineralized by strain S12 under anaerobic conditions. But after re-aeration of the decolorized culture, RP-2
was mineralized completely by this microorganism, but the consumption of RP-1 was not observed. Ames test showed that amaranth
had mutagenic but no cytotoxic potential. The mutagenic potential was relieved after the anaerobic treatment with strain S12
as the mutagenic effect of the two reduction products from amaranth was not detected by Ames test. Thus, the ability of strain
S12 to reduce and partially mineralize the naphthylaminesulfonic azo dye efficiently was demonstrated, which can potentially
be used to biodegrade and detoxify wastewater containing azo dyes using an alternating anaerobic/aerobic treatment procedure. 相似文献
3.
Azo Reductase Activity of Intact Saccharomyces cerevisiae Cells Is Dependent on the Fre1p Component of Plasma Membrane Ferric Reductase 总被引:1,自引:0,他引:1
Patrícia A. Ramalho Sandra Paiva A. Cavaco-Paulo Margarida Casal M. Helena Cardoso M. Teresa Ramalho 《Applied microbiology》2005,71(7):3882-3888
Unspecific bacterial reduction of azo dyes is a process widely studied in correlation with the biological treatment of colored wastewaters, but the enzyme system associated with this bacterial capability has never been positively identified. Several ascomycete yeast strains display similar decolorizing behaviors. The yeast-mediated process requires an alternative carbon and energy source and is independent of previous exposure to the dyes. When substrate dyes are polar, their reduction is extracellular, strongly suggesting the involvement of an externally directed plasma membrane redox system. The present work demonstrates that, in Saccharomyces cerevisiae, the ferric reductase system participates in the extracellular reduction of azo dyes. The S. cerevisiae Δfre1 and Δfre1 Δfre2 mutant strains, but not the Δfre2 strain, showed much-reduced decolorizing capabilities. The FRE1 gene complemented the phenotype of S. cerevisiae Δfre1 cells, restoring the ability to grow in medium without externally added iron and to decolorize the dye, following a pattern similar to the one observed in the wild-type strain. These results suggest that under the conditions tested, Fre1p is a major component of the azo reductase activity. 相似文献
4.
The function of cytoplasmic flavin reductases in the reduction of azo dyes by bacteria 总被引:4,自引:0,他引:4
A flavin reductase, which is naturally part of the ribonucleotide reductase complex of Escherichia coli, acted in cell extracts of recombinant E. coli strains under aerobic and anaerobic conditions as an "azo reductase." The transfer of the recombinant plasmid, which resulted in the constitutive expression of high levels of activity of the flavin reductase, increased the reduction rate for different industrially relevant sulfonated azo dyes in vitro almost 100-fold. The flavin reductase gene (fre) was transferred to Sphingomonas sp. strain BN6, a bacterial strain able to degrade naphthalenesulfonates under aerobic conditions. The flavin reductase was also synthesized in significant amounts in the Sphingomonas strain. The reduction rates for the sulfonated azo compound amaranth were compared for whole cells and cell extracts from both recombinant strains, E. coli, and wild-type Sphingomonas sp. strain BN6. The whole cells showed less than 2% of the specific activities found with cell extracts. These results suggested that the cytoplasmic anaerobic "azo reductases," which have been described repeatedly in in vitro systems, are presumably flavin reductases and that in vivo they have insignificant importance in the reduction of sulfonated azo compounds. 相似文献
5.
The Function of Cytoplasmic Flavin Reductases in the Reduction of Azo Dyes by Bacteria 总被引:11,自引:1,他引:10 下载免费PDF全文
A flavin reductase, which is naturally part of the ribonucleotide reductase complex of Escherichia coli, acted in cell extracts of recombinant E. coli strains under aerobic and anaerobic conditions as an “azo reductase.” The transfer of the recombinant plasmid, which resulted in the constitutive expression of high levels of activity of the flavin reductase, increased the reduction rate for different industrially relevant sulfonated azo dyes in vitro almost 100-fold. The flavin reductase gene (fre) was transferred to Sphingomonas sp. strain BN6, a bacterial strain able to degrade naphthalenesulfonates under aerobic conditions. The flavin reductase was also synthesized in significant amounts in the Sphingomonas strain. The reduction rates for the sulfonated azo compound amaranth were compared for whole cells and cell extracts from both recombinant strains, E. coli, and wild-type Sphingomonas sp. strain BN6. The whole cells showed less than 2% of the specific activities found with cell extracts. These results suggested that the cytoplasmic anaerobic “azo reductases,” which have been described repeatedly in in vitro systems, are presumably flavin reductases and that in vivo they have insignificant importance in the reduction of sulfonated azo compounds. 相似文献
6.
Halomonas sp strain GTW was newly isolated from coastal sediments contaminated by chemical wastewater and was identified to be a member
of the genus Halomonas by 16S rDNA sequence analysis and physical and biochemical tests. The optimal decolorization conditions were as follows:
temperature 30°C, pH 6.5.0–8.5, NaCl 10–20% (w/v) and the optimal carbon source was yeast exact. The results of experiments
demonstrated that the bacteria could decolorize different azo dyes under high salt concentration conditions, and the decolorization
rate of five tested azo dyes could be above 90% in 24 h. The exploitation of the salt-tolerant bacteria in the bio-treatment
system would be a great improvement of conventional biological treatment systems and the bio-treatment concept. 相似文献
7.
Identification of Quinoide Redox Mediators That Are Formed during the Degradation of Naphthalene-2-Sulfonate by Sphingomonas xenophaga BN6 总被引:1,自引:0,他引:1 下载免费PDF全文
Andreas Keck Jrg Rau Thorsten Reemtsma Ralf Mattes Andreas Stolz Joachim Klein 《Applied microbiology》2002,68(9):4341-4349
During aerobic degradation of naphthalene-2-sulfonate (2NS), Sphingomonas xenophaga strain BN6 produces redox mediators which significantly increase the ability of the strain to reduce azo dyes under anaerobic conditions. It was previously suggested that 1,2-dihydroxynaphthalene (1,2-DHN), which is an intermediate in the degradative pathway of 2NS, is the precursor of these redox mediators. In order to analyze the importance of the formation of 1,2-DHN, the dihydroxynaphthalene dioxygenase gene (nsaC) was disrupted by gene replacement. The resulting strain, strain AKE1, did not degrade 2NS to salicylate. After aerobic preincubation with 2NS, strain AKE1 exhibited much higher reduction capacities for azo dyes under anaerobic conditions than the wild-type strain exhibited. Several compounds were present in the culture supernatants which enhanced the ability of S. xenophaga BN6 to reduce azo dyes under anaerobic conditions. Two major redox mediators were purified from the culture supernatants, and they were identified by high-performance liquid chromatography-mass spectrometry and comparison with chemically synthesized standards as 4-amino-1,2-naphthoquinone and 4-ethanolamino-1,2-naphthoquinone. 相似文献
8.
Muyang He Liang Tan Shuxiang Ning Li Song Shengnan Shi 《World journal of microbiology & biotechnology》2017,33(2):39
Biological aerated filters (BAFs) were constructed and operated for assessing the effectiveness of bacterial community bioaugmented by a yeast Magnusiomyces ingens LH-F1 for treatment of azo dye Acid Red B (ARB). Dynamics of both bacterial and fungal communities were analyzed through MiSeq sequencing method. The results showed that the bioaugmented BAF displayed obviously better performance for decolorization, COD removal and detoxification of ARB wastewater than the other two which were inoculated with activated sludge (AS) and single M. ingens LH-F1, respectively. Moreover, the bioaugmented BAF also exhibited higher tolerance and stability to shock loading. MiSeq sequencing results demonstrated that both of bacterial and fungal communities remarkably shifted with operation conditions, and the increasing fungal diversity in the bioaugmented BAF was probably related to the relatively high biodegradation and detoxification efficiency. Furthermore, M. ingens LH-F1 survived in the bioaugmented BAF and became one of the dominant fungal species. Therefore, bioaugmentation with yeast M. ingens LH-F1 was successful for improving traditional biological processes aiming at treatment of azo compounds. This method was also potentially useful and meaningful for treating other recalcitrant organic pollutants in practical applications. 相似文献
9.
Isolation of a Bacterial Strain with the Ability To Utilize the Sulfonated Azo Compound 4-Carboxy-4′-Sulfoazobenzene as the Sole Source of Carbon and Energy 下载免费PDF全文
Silke Blümel Matthias Contzen Martina Lutz Andreas Stolz Hans-Joachim Knackmuss 《Applied microbiology》1998,64(6):2315-2317
A bacterial strain (strain S5) which grows aerobically with the sulfonated azo compound 4-carboxy-4′-sulfoazobenzene as the sole source of carbon and energy was isolated. This strain was obtained by continuous adaptation of “Hydrogenophaga palleronii” S1, which has the ability to grow aerobically with 4-aminobenzenesulfonate. Strain S5 probably cleaves 4-carboxy-4′-sulfoazobenzene reductively under aerobic conditions to 4-aminobenzoate and 4-aminobenzene-sulfonate, which are mineralized by previously established degradation pathways. 相似文献
10.
Shewanella decolorationis S12 is capable of high rates of azo dye decolorization and dissimilatory Fe(III) reduction. Under anaerobic conditions, when
Fe(III) and azo dye were copresent in S12 cultures, dissimilatory Fe(III) reduction and azo dye biodecolorization occurred
simultaneously. Furthermore, the dye decolorization was enhanced by the presence of Fe(III). When 1 mM Fe(III) was added,
the methyl red decolorizing efficiency was 72.1% after cultivation for 3 h, whereas the decolorizing efficiency was only 60.5%
in Fe(III)-free medium. The decolorizing efficiencies increased as the concentration of Fe(III) was increased from 0 to 6 mM.
Enzyme activities, which mediate the dye decolorization and Fe(III) reduction, were not affected by preadaption of cells to
Fe(III) and azo dye nor by the addition of chloramphenicol. Both the Fe(III) reductase and the azo reductase were membrane
associated. The respiratory electron transport chain inhibitors metyrapone, dicumarol, and stigmatellin showed significantly
different effects on Fe(III) reduction than on azo dye decolorization. 相似文献
11.
Biodegradation of textile azo dye by <Emphasis Type="Italic">Shewanella decolorationis</Emphasis> S12 under microaerophilic conditions 总被引:3,自引:0,他引:3
The complete biodegradation of azo dye, Fast Acid Red GR, was observed under microaerophilic conditions by Shewanella decolorationis S12. Although the highest decolorizing rate was measured under anaerobic condition and the highest biomass was obtained under
aerobic condition, a further biodegradation of decolorizing products can only be achieved under microaerophilic conditions.
Under microaerophilic conditions, S. decolorationis S12 could use a range of carbon sources for azo dye decolorization, including lactate, formate, glucose and sucrose, with
lactate being the optimal carbon source. Sulfonated aromatic amines were not detected during the biotransformation of Fast
Acid Red GR, while H2S formed. The decolorizing products, aniline, 1,4-diaminobenzene and 1-amino-2-naphthol, were followed by complete biodegradation
through catechol and 4-aminobenzoic acid based on the analysis results of GC-MS and HPLC. 相似文献
12.
Identification of quinoide redox mediators that are formed during the degradation of naphthalene-2-sulfonate by Sphingomonas xenophaga BN6 总被引:2,自引:0,他引:2
Keck A Rau J Reemtsma T Mattes R Stolz A Klein J 《Applied and environmental microbiology》2002,68(9):4341-4349
During aerobic degradation of naphthalene-2-sulfonate (2NS), Sphingomonas xenophaga strain BN6 produces redox mediators which significantly increase the ability of the strain to reduce azo dyes under anaerobic conditions. It was previously suggested that 1,2-dihydroxynaphthalene (1,2-DHN), which is an intermediate in the degradative pathway of 2NS, is the precursor of these redox mediators. In order to analyze the importance of the formation of 1,2-DHN, the dihydroxynaphthalene dioxygenase gene (nsaC) was disrupted by gene replacement. The resulting strain, strain AKE1, did not degrade 2NS to salicylate. After aerobic preincubation with 2NS, strain AKE1 exhibited much higher reduction capacities for azo dyes under anaerobic conditions than the wild-type strain exhibited. Several compounds were present in the culture supernatants which enhanced the ability of S. xenophaga BN6 to reduce azo dyes under anaerobic conditions. Two major redox mediators were purified from the culture supernatants, and they were identified by high-performance liquid chromatography-mass spectrometry and comparison with chemically synthesized standards as 4-amino-1,2-naphthoquinone and 4-ethanolamino-1,2-naphthoquinone. 相似文献
13.
Hou X 《Applied microbiology and biotechnology》2012,94(1):205-214
A cost-effective conversion of lignocellulosic biomass into bioethanol requires that the xylose released from the hemicellulose
fraction (20–40% of biomass) can be fermented. Baker’s yeast, Saccharomyces cerevisiae, efficiently ferments glucose but it lacks the ability to ferment xylose. Xylose-fermenting yeast such as Pichia stipitis requires accurately controlled microaerophilic conditions during the xylose fermentation, rendering the process technically
difficult and expensive. In this study, it is demonstrated that under anaerobic conditions Spathaspora passalidarum showed high ethanol production yield, fast cell growth, and rapid sugar consumption with xylose being consumed after glucose
depletion, while P. stipitis was almost unable to utilize xylose under these conditions. It is further demonstrated that for S. passalidarum, the xylose conversion takes place by means of NADH-preferred xylose reductase (XR) and NAD+-dependent xylitol dehydrogenase (XDH). Thus, the capacity of S. passalidarum to utilize xylose under anaerobic conditions is possibly due to the balance between the cofactor’s supply and demand through
this XR–XDH pathway. Only few XRs with NADH preference have been reported so far. 2-Deoxy glucose completely inhibited the
conversion of xylose by S. passalidarum under anaerobic conditions, but only partially did that under aerobic conditions. Thus, xylose uptake by S. passalidarum may be carried out by different xylose transport systems under anaerobic and aerobic conditions. The presence of glucose
also repressed the enzymatic activity of XR and XDH from S. passalidarum as well as the activities of those enzymes from P. stipitis. 相似文献
14.
Removal of azo dyes from the effluent generated by textile industries is rather difficult. Azo dyes represent a major class
of synthetic colorants that are both mutagenic and carcinogenic. Galactomyces geotrichum MTCC 1360, a yeast species, showed more than 96% decolorization of the azo dye Remazol Red (50 mg/L) within 36 h at 30°C
and pH 11.0 under static condition with a significant reduction in the chemical oxygen demand (62%) and total organic carbon
(41%). Peptone (5.0 g/L), rice husk (10 g/L extract), and ammonium chloride (5.0 g/L) were found to be more significant among
the carbon and nitrogen sources used. The presence of tyrosinase, NADH-DCIP reductase, riboflavin reductase and induction
in azo reductase and laccase activity during decolorization indicated their role in degradation. High performance thin layer
chromatography analysis revealed the degradation of Remazol Red into different metabolites. Fourier transform infrared spectroscopy
and high performance liquid chromatography analysis of samples before and after decolorization confirmed the biotransformation
of dye. Atomic absorption spectroscopy analysis revealed a less toxic effect of the metabolites on iron uptake by Sorghum vulgare and Phaseolus mungo than Remazol Red dye. Remazol Red showed an inhibitory effect on iron uptake by chelation and an immobilization of iron,
whereas its metabolites showed no chelation as well as immobilization of iron. Phytotoxicity study indicated the conversion
of complex dye molecules into simpler oxidizable products which had a less toxic nature. 相似文献
15.
脱色希瓦氏菌(Shewanella decolorationis)S12T的脱色特性 总被引:4,自引:0,他引:4
从印染废水活性污泥中分离到一株高效染料脱色菌,经鉴定该菌株为希瓦氏菌属的一个新种,命名为脱色希瓦氏菌(Shewanelladecolorationis)S12T。该菌株在偶氮染料浓度为50mg/L的培养基中培养4h后,染料去除率达到96%,对偶氮染料的最高脱色浓度达到2000mg/L。在浓度为500mg/L的偶氮染料平板上生长4d后,可观察到明显的脱色圈。全波长光谱扫描的结果表明希瓦氏菌S12T以生物降解的方式对偶氮染料进行脱色。希瓦氏菌S12T的脱色酶为组成型的胞内酶。 相似文献
16.
Effects of electron donors and acceptors on anaerobic reduction of azo dyes by <Emphasis Type="Italic">Shewanella decolorationis</Emphasis> S12 总被引:1,自引:0,他引:1
Shewanella decolorationis S12 was able to reduce various azo dyes in a defined medium with formate, lactate, and pyruvate or H2 as electron donors under anaerobic conditions. Purified membranous, periplasmic, and cytoplasmic fractions from strain S12
analyzed, respectively, only membranous fraction was capable of reducing azo dye in the presence of electron donor, indicating
that the enzyme system for anaerobic azoreduction was located on cellular membrane. Respiratory inhibitor Cu2+, dicumarol, stigmatellin, and metyrapone inhibited anaerobic azoreduction by purified membrane fraction, suggesting that
the bacterial anaerobic azoreduction by strain S12 was a biochemical process that oxidizes the electron donors and transfers
the electrons to the acceptors through a multicompound system related to electron transport chain. Dehydrogenases, cytochromes,
and menaquinones were essential electron transport components for the azoreduction. The electron transport process for azoreduction
was almost fully inhibited by O2, 6 mM of , and 0.9 mM of , but not by 10 mM of Fe3+. The inhibition may be a result from the competition for electrons from electron donors. These findings impact on the understanding
of the mechanism of bacterial anaerobic azoreduction and have implication for improving treatment methods of wastewater contaminated
by azo dyes. 相似文献
17.
《FEMS yeast research》2005,5(3):213-230
The first protein map of an ale-fermenting yeast is presented in this paper: 205 spots corresponding to 133 different proteins were identified. Comparison of the proteome of this ale strain with a lager brewing yeast and the Saccharomyces cerevisiae strain S288c confirmed that this ale strain is much closer to S288c than the lager strain at the proteome level. The dynamics of the ale-brewing yeast proteome during production-scale fermentation was analysed at the beginning and end of the first and the third usage of the yeast (called generation in the brewing industry). During the first generation, most changes were related to the switch from aerobic propagation to anaerobic fermentation. Fewer changes were observed during the third generation but certain stress-response proteins such as Hsp26p, Ssa4p and Pnc1p exhibited constitutive expression in subsequent generations. The ale brewing yeast strain appears to be quite well adapted to fermentation conditions and stresses. 相似文献
18.
Shewanella decolorationis S12 is capable of carrying out anaerobic respiration using azo dyes and Fe (III) citrate as electron acceptors. In the present
study, proteomic techniques including two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization
time-of-flight/time-of-flight mass spectrometry were used to analyze the similarity and the dissimilarity of the membrane
proteins isolated from strain S12 cells grown in amaranth or Fe (III) citrate with defined inorganic salt medium. The cells
of strain S12 grown under a saturated dissolved oxygen condition served as controls. This is the first work that made the
comparative analysis of cell membranous proteomics of strain S12 grown with azo compound or Fe (III) citrate as a sole terminal
electron acceptor. The results showed that most of the membrane proteins of strain S12 under azo respiration are similar to
those under Fe (III) respiration, but dissimilar from those of oxygen-grown cells. FdnH and FrdB were expressed specifically
in azo respiration. NqrA-2, DctP, and hypothetical protein SO_4719 showed relative overexpression in azo respiration compared
with Fe (III) respiration. OmpA family protein SO_3545 was detected to be specific to Fe (III) respiration. Furthermore, ArgF,
SdhA, and HoxK were expressed markedly in both amaranth- and Fe (III) citrate-grown cultures compared with oxygen-grown cultures. 相似文献
19.
从浙江某污水处理厂的活性污泥中筛选出若干株在高pH条件下对偶氮染料酸性大红GR有脱色能力的菌株,经脱色验证得到一株具有高效脱色活性的菌株Z1,经鉴定为巴斯德葡萄球菌(Staphylococcus pasteuri),并对此菌株的脱色特性进行了初步研究。结果表明,在厌氧条件下,Z1在pH7~12,40h对50mg/L的酸性大红GR脱色率均可达90%以上。该菌株对染料有较强的耐受力,在酸性大红GR浓度为300mg/L时,48h的脱色率仍可达93%。此外,该菌株能够对多种偶氮染料脱色,具有较好的脱色广谱性,有望应用于处理工业废水中的偶氮染料。 相似文献
20.
Oxygen-Insensitive Nitroreductases NfsA and NfsB of Escherichia coli Function under Anaerobic Conditions as Lawsone-Dependent Azo Reductases 下载免费PDF全文
Quinones can function as redox mediators in the unspecific anaerobic reduction of azo compounds by various bacterial species. These quinones are enzymatically reduced by the bacteria and the resulting hydroquinones then reduce in a purely chemical redox reaction the azo compounds outside of the cells. Recently, it has been demonstrated that the addition of lawsone (2-hydroxy-1,4-naphthoquinone) to anaerobically incubated cells of Escherichia coli resulted in a pronounced increase in the reduction rates of different sulfonated and polymeric azo compounds. In the present study it was attempted to identify the enzyme system(s) responsible for the reduction of lawsone by E. coli and thus for the lawsone-dependent anaerobic azo reductase activity. An NADH-dependent lawsone reductase activity was found in the cytosolic fraction of the cells. The enzyme was purified by column chromatography and the amino-terminal amino acid sequence of the protein was determined. The sequence obtained was identical to the sequence of an oxygen-insensitive nitroreductase (NfsB) described earlier from this organism. Subsequent biochemical tests with the purified lawsone reductase activity confirmed that the lawsone reductase activity detected was identical with NfsB. In addition it was proven that also a second oxygen-insensitive nitroreductase of E. coli (NfsA) is able to reduce lawsone and thus to function under adequate conditions as quinone-dependent azo reductase. 相似文献