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N. Rashevsky 《Protoplasma》1933,20(1):125-130
Summary A mathematical analysis of the diffusion of oxygen into a cell is made, under the assumption, that the true rate of consumption
of oxygen by protoplasm is constant and independent on the oxygen pressure. The results are compared with experimental data.
For unfertilizedArbacia eggs and for bacteria the experimental curves cannot be represented by the theory. Some additional assumptions, like those
proposed byGerard, seem unavoidable. For fertilizedArbacia eggs a good agreement may however be obtained, by taking for the diffusion coefficient of oxygen trough the protoplasm 7×10−7 cm2·min−1, and for the permeability of the cell surface to oxygen 4.25×10−4cm·min−1.
With 1 Text-figure 相似文献
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Human spermatozoan oxygen consumption was measured in semen serially diluted with its own plasma, and shortly after serial dilution of washed spermatozoa in an artificial medium. In both media, per-sperm oxygen uptake rose significantly when sperm concentration fell below the range of 15-25 X 10(6) cells/ml. Oxygen uptake was higher in the artificial medium, regardless of sperm concentration. Sperm oxygen consumption in the artificial medium declined over times short enough that no change in the percentage of motile spermatozoa was detected. 相似文献
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When subjected to a series of elevated Al and H+ concentrations spanning environmentally relevant levels, the dragonfly Libellula julia respired at a rate lower than the controls. This trend was consistent at all levels but only attained significance (p < 0.05) at pH 4.0 with A1 levels of 3.0 and 30 mg 1–1. Low pH alone does not depress respiratory rates as greatly as Al and low pH combined. The authors speculate on some apparent inconsistencies found in the literature. 相似文献
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The generally accepted view that rates of oxygen consumption of tissues and poikilotherms increase regularly with rising temperature was subjected to careful examination using brain slices and skin of rats and nine different species of aquatic and terrestrial animals. It was found that, although there are statements in the literature to the contrary, the influence of temperature is a regular one and respiration increases with rising temperature so that when rates of oxygen consumption are plotted against temperature the resulting curve is regular without dips or peaks except the maximum expected at the optimum temperature. 相似文献
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Oxygen demand and long term changes of profundal zoobenthos 总被引:3,自引:3,他引:0
Petur M. Jónasson 《Hydrobiologia》1984,115(1):121-126
The paper attempts to combine the low oxygen content of the hypolimnion during stratification and the oxygen uptake of zoobenthos. Data of declining oxygen content in the hypolimnion and critical limits of respiration are combined for Chironomus anthracinus, Potamothrix hammoniensis and three species of Pisidium, P. casertanum, P. subtruncatum and P. henslowanum. The respiratory adaptation to low oxygen content influences both growth and population dynamics of the different species. The results have important bearing on eutrophication of the Lake Esrom ecosystem and temperate eutrophic lakes in general as well as the composition of profundal zoobenthos and its population dynamics.Publication No. 389 from Freshwater-Biological Laboratory, University of Copenhagen. 相似文献
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A technique for measuring the oxygen consumption of small laboratory animals suitable for pharmacological and toxicological studies is described. The oxygen consumption of the animal in the respiratory chamber is compensated by means of a photoelectrically operating servo mechanism from an oxygen reservoir. The oxygen supplied is directly recorded via a transducer by an X-Y recorder. The carbon dioxide exhaled by the animal is entirely absorbed. Composition and pressure of air in the respiratory chamber correspond to the physiological medium. The usefulness of this technique is demonstrated by investigations on the effects of epinephrine and morphine on the oxygen consumption of rats. 相似文献
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Estimates ofin situ nitrifying activity have been made in the hypolimnetic water column and surface 1.0 cm of profundal sediments at 2 sites in Grasmere, a mesotrophic lake in the English Lake District. Increases of nitrate concentrations were used to estimate nitrification in the water column whereas a mini-core technique, involving the use of a nitrification inhibitor (allylthiourea), was used to estimate the rate in surface sediments. The pattern of oxygen depletion in the water column was used to estimate the maximum depth to which sediments affect the overlying water. Nitrification in the sediment and in the water column made approximately equal contributions to the total areal oxygen deficit and, as a whole, nitrification accounted for 15–20% of the total oxygen depletion. There was no significant difference in oxygen depletion due to nitrification between the 2 sites. Attempts were made, using the nitrification potential technique, to determine the depth distribution of nitrifying activity in the surface 1.0 cm of sediment. 相似文献
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The kinetics of oxygen binding of Helix pomatia α-hemocyanin has been studied at low and intermediate levels of ligand saturation, under conditions in which oxygen binding is highly co-operative. Temperature-jump relaxation spectra are heterogeneous and can be resolved into a slow and a fast phase. The latter is related to a bimolecular reaction, i.e. the binding of oxygen. At very low degrees of fractional saturation (<0.15) the reactant concentration-dependence of the faster relaxation rate allows the combination and dissociation rate constants of the low affinity or T-state to be estimated as 1.3 × 106m?1 s?1 and 300 s?1, respectively. A possible interpretation of the slow component in the relaxation spectrum is discussed.In stopped-flow experiments, after mixing deoxyhemocyanin with oxygen-containing buffer, most of the binding process to the T-state is lost in the dead time. The observed initial rates of oxygen binding are between 15 and 120 s?1. depending on the oxygen concentration, and may reflect the rate of the allosteric change from a low to a high affinity state (T→R transition), which is slower than oxygen binding.Similarities and differences in the overall kinetic properties of small and giant respiratory proteins, i.e. hemoglobin and hemocyanin, are discussed. 相似文献
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We quantified the role of a main food resource, sedimenting organic matter (SOM), relative to oxygen (DO) and temperature (TEMP) in structuring profundal macroinvertebrate assemblages in boreal lakes. SOM from 26 basins of 11 Finnish lakes was analysed for quantity (sedimentation rates), quality (C:N:P stoichiometry) and origin (carbon stable isotopes, δ13C). Hypolimnetic oxygen and temperature were measured from each site during summer stratification. Partial canonical correspondence analysis (CCA) and partial regression analyses were used to quantify contributions of SOM, DO and TEMP to community composition and three macroinvertebrate metrics. The results suggested a major contribution of SOM in regulating the community composition and total biomass. Oxygen best explained the Shannon diversity, whereas TEMP had largest contribution to the variation of Benthic Quality Index. Community composition was most strongly related to δ13C of SOM. Based on additional δ13C and stoichiometric analyses of chironomid taxa, marked differences were apparent in their utilization of SOM and body stoichiometry; taxa characteristic of oligotrophic conditions exhibited higher C:N ratios and lower C:P and N:P ratios compared to the species typical of eutrophic lakes. The results highlight the role of SOM in regulating benthic communities and the distributions of individual species, particularly in oligotrophic systems. 相似文献
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Using laser Raman spectroscopy, we are able to study conformational changes that occur as previously-dried hen egg-white lysozyme is sequentially rehydrated. Parallel n.m.r. exchangeability studies enable us to monitor flexibility changes also during this rehdyration. The results are consistent with a general loosening up of the protein at a water content of ~0.08 g water/g protein, followed by (probably small) local conformational changes. The enzyme regains its activity only after both these processes have gone to completion; thus these solvent-related changes may be necessary before activity can recommence. 相似文献
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1. Benthic macroinvertebrates (MI) are commonly used to assess freshwater ecosystems with the reference condition approach. Such assessments necessitate control for natural community variation, either by categorical typologies or by predictive models that have been widely and successfully developed for running water biota but not previously for lake profundal invertebrates. 2. We evaluated four modelling techniques [multivariate regression tree (MRT), limiting environmental differences, nonparametric multiplicative regression (NPMR) and River Invertebrate Prediction And Classification System (RIVPACS) and the operative Finnish lake typology for assessing taxonomic completeness (observed‐to‐expected number of taxa, O/E) of profundal MI assemblages. We used data from 74 and 33 minimally disturbed reference lake basins for calibration and validation of the approaches, respectively, and 72 test basins subject to various anthropogenic pressures to evaluate sensitivity to detect impact. Either all predicted taxa (threshold probability of capture Pt = 0+) or only those predicted to be captured with ≥0.25 probability were used to calculate O/E. 3. With Pt = 0.25, all four modelling approaches were accurate (mean O/E = 0.966–1.053) but imprecise (SD of O/E = 0.279–0.304) in predicting the fauna actually observed in validation sites. All models were subtly more precise than a null model (mean 1.038, SD 0.343) or the typology (1.046, 0.327). The taxon‐specific NPMR model was slightly more precise than the other three models based on site groupings. 4. The O/E values correlated relatively weakly (r = 0.55–0.86) among the approaches, which thus produced contrasting lake‐specific assessments, despite their seemingly comparable performances. Indeed, typology, suggesting that MI assemblages were impaired in 56% of test sites, was more sensitive than the other approaches (26–46%) as an indicator of human‐induced deterioration. However, this greater ostensible sensitivity seemed to be biased, as lake morphometry, a main driver of natural community variation, remained uncontrolled by the typology. 5. Generally, our exercise illustrates the inconclusiveness of the common validation criteria for the assessment methods. The apparent poor predictability of the profundal fauna, irrespective of the method, may partly stem from large observation error, which could be alleviated by more intensive sampling. However, instead of an O/E‐taxa index, some other metric encompassing quantitative aspects might be preferable for assessing these species‐poor communities. 相似文献
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M J Stock 《Journal of applied physiology》1975,39(5):849-850
An apparatus suitable for the continuous measurement of oxygen consumption of rats and mice is described. The system uses a motorized syringe dispenser to deliver fixed volumes of oxygen to a closed animal chamber. The dispenser is controlled by a micro-differential pressure switch to maintain chamber pressure slightly above ambient. The rate of oxygen consumption is determined by timing the interval between successive operations of the dispenser. The system has proved suitable for a range of experimental conditions and treatments. 相似文献
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Rebel A Lenz C Krieter H Waschke KF Van Ackern K Kuschinsky W 《American journal of physiology. Heart and circulatory physiology》2001,280(6):H2591-H2597
We addressed the question to which extent cerebral blood flow (CBF) is maintained when, in addition to a high blood viscosity (Bvis) arterial oxygen content (CaO2) is gradually decreased. CaO2) was decreased by hemodilution to hematocrits (Hct) of 30, 22, 19, and 15% in two groups. One group received blood replacement (BR) only and served as the control. The second group received an additional high viscosity solution of polyvinylpyrrolidone (BR/PVP). Bvis was reduced in the BR group and was doubled in the BR/PVP. Despite different Bvis, CBF did not differ between BR and BR/PVP rats at Hct values of 30 and 22%, indicating a complete vascular compensation of the increased Bvis at decreased CaO2. At an Hct of 19%, local cerebral blood flow (LCBF) in some brain structures was lower in BR/PVP rats than in BR rats. At the lowest Hct of 15%, LCBF of 15 brain structures and mean CBF were reduced in BR/PVP. The resulting decrease in cerebral oxygen delivery in the BR/PVP group indicates a global loss of vascular compensation. We concluded that vasodilating mechanisms compensated for Bvis increases thereby maintaining constant cerebral oxygen delivery. Compensatory mechanisms were exhausted at a Hct of 19% and lower as indicated by the reduction of CBF and cerebral oxygen delivery. 相似文献
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Green JA Halsey LG Butler PJ Holder RL 《American journal of physiology. Regulatory, integrative and comparative physiology》2007,292(5):R2028-R2038
How animals manage their oxygen stores during diving and other breath-hold activities has been a topic of debate among physiologists for decades. Specifically, while the behavior of free-ranging diving animals suggests that metabolism during submersion must be primarily aerobic in nature, no studies have been able to determine their rate of oxygen consumption during submersion (Vo(2)d) and hence prove that this is the case. In the present study, we combine two previously used techniques and develop a new model to estimate Vo(2)d accurately and plausibly in a free-ranging animal and apply it to data for macaroni penguins (Eudyptes chrysolophus) as an example. For macaroni penguins at least, Vo(2)d can be predicted by measuring heart rate during the dive cycle and the subsequent surface interval duration. Including maximum depth of the dive improves the accuracy of these predictions. This suggests that energetically demanding locomotion events within the dive combine with the differing buoyancy and locomotion costs associated with traveling to depth to influence its cost in terms of oxygen use. This will in turn effect the duration of the dive and the duration of the subsequent recovery period. In the present study, Vo(2)d ranged from 4 to 28 ml.min(-1).kg(-1), indicating that, at least as far as aerobic metabolism was concerned, macaroni penguins were often hypometabolic, with rates of oxygen consumption usually below that for this species resting in water (25.6 ml.min(-1).kg(-1)) and occasionally lower than that while resting in air (10.3 ml.min(-1).kg(-1)). 相似文献
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Hendrikus J. Laanbroek Paul L. E. Bodelier Saskia Gerards 《Archives of microbiology》1994,161(2):156-162
Chemolithotrophic ammonium- and nitrite-oxidizing bacteria are dependent on the presence of oxygen for the production of nitrite and nitrate, respectively. In oxygen-limited environments, they have to compete with each other as well as with other organotrophic bacteria for the available oxygen. The outcome of the competition will be determined by their specific affinities for oxygen as well as by their population sizes. The effect of mixotrophic growth by the nitrite-oxidizing Nitrobacter hamburgensis on the competition for limiting amounts of oxygen was studied in mixed continuous culture experiments with the ammonium-oxidizing Nitrosomonas europaea at different levels of oxygen concentrations.The specific affinity for oxygen of N. europaea was in general higher than of N. hamburgensis. In transient state experiments, when oxic conditions were switched to anoxic, N. hamburgensis was washed out and nitrite accumulated. However, grown at low oxygen concentration, the specific affinity for oxygen of N. hamburgensis increased and became as great as that of N. europaea. Due to its larger population size, the nitrite-oxidizing bacterium became the better competitor for oxygen and ammonium accumulated in the fermentor. It is suggested that continuously oxygen-limited environments present a suitable ecological niche for the nitrite-oxidizing N. hamburgensis. 相似文献