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1.
To examine whether dihydrofolate reductase (DHFR) from deep-sea bacteria has undergone molecular evolution to adapt to high-pressure
environments, we cloned eight DHFRs from Shewanella species living in deep-sea and ambient atmospheric-pressure environments, and subsequently purified six proteins to compare
their structures, stabilities, and functions. The DHFRs showed 74–90% identity in primary structure to DHFR from S. violacea, but only 55% identity to DHFR from Escherichia coli (ecDHFR). Far-ultraviolet circular dichroism and fluorescence spectra suggested that the secondary and tertiary structures
of these DHFRs were similar. In addition, no significant differences were found in structural stability as monitored by urea-induced
unfolding and the kinetic parameters, K
m and k
cat; although the DHFRs from Shewanella species were less stable and more active (2- to 4-fold increases in k
cat/ K
m) than ecDHFR. Interestingly, the pressure effects on enzyme activity revealed that DHFRs from ambient-atmospheric species
are not necessarily incompatible with high pressure, and DHFRs from deep-sea species are not necessarily tolerant of high
pressure. These results suggest that the DHFR molecule itself has not evolved to adapt to high-pressure environments, but
rather, those Shewanella species with enzymes capable of retaining functional activity under high pressure migrated into the deep-sea. 相似文献
2.
Two cytochromes c5 (SBcyt c and SVcyt c) have been derived from Shewanella living in the deep-sea, which is a high pressure environment, so it could be that these proteins are more stable at high pressure than at atmospheric pressure, 0.1 MPa. This study, however, revealed that SBcyt c and SVcyt c were more stable at 0.1 MPa than at higher pressure. In addition, at 0.1–150 MPa, the stability of SBcyt c and SVcyt c was higher than that of homologues from atmospheric-pressure Shewanella, which was due to hydrogen bond formation with the heme in the former two proteins. This study further revealed that cytochrome c551 (PMcyt c) of deep-sea Pseudomonas was more stable than a homologue of atmospheric-pressure Pseudomonas aeruginosa, and that specific hydrogen bond formation with the heme also occurred in the former. Although SBcyt c and SVcyt c, and PMcyt c are phylogenetically very distant, these deep-sea cytochromes c are commonly stabilized through hydrogen bond formation. 相似文献
3.
The sediments of a deep-sea hypersaline and sulfidic Mediterranean basin have yielded an unexpected discovery, the first multicellular
animals living entirely without oxygen. Reported by Danovaro et al. in BMC Biology, these three new species of Loricifera add a new and remarkable dimension to anoxic ecosystems previously
thought to support only unicellular life. 相似文献
4.
Monomeric cytochrome c5 from deep-sea piezophilic Shewanella violacea (SVcyt c5) was stable against heat and denaturant compared with the homologous protein from shallow-sea piezo-sensitive Shewanella livingstonensis (SLcyt c5). Here, the SVcyt c5 crystal structure revealed that the Lys-50 side chain on the flexible loop formed a hydrogen bond with heme whereas that of corresponding hydrophobic Leu-50 could not form such a bond in SLcyt c5, which appeared to be one of possible factors responsible for the difference in stability between the two proteins. This structural insight was confirmed by a reciprocal mutagenesis study on the thermal stability of these two proteins. As SVcyt c5 was isolated from a deep-sea piezophilic bacterium, the present comparative study indicates that adaptation of monomeric SVcyt c5 to high pressure environments results in stabilization against heat. 相似文献
5.
Visual systems in deep-sea fishes have been previously studied from a photobiological aspect; however, those of deep-sea fish inhabiting the hydrothermal vents are far less understood due to sampling difficulties. In this study, we analyzed the visual pigment of a deep-sea snailfish, Careproctus rhodomelas, discovered and collected only near the hydrothermal vents of oceans around Japan. Proteins were solubilized from the C. rhodomelas eyeball and subjected to spectroscopic analysis, which revealed the presence of a pigment characterized by an absorption maximum (λ max) at 480 nm. Immunoblot analysis of the ocular protein showed a rhodopsin-like immunoreactivity. We also isolated a retinal cDNA encoding the entire coding sequence of putative C. rhodomelas rhodopsin (CrRh). HEK293EBNA cells were transfected with the CrRh cDNA and the proteins extracted from the cells were subjected to spectroscopic analysis. The recombinant CrRh showed the absorption maximum at 480 nm in the presence of 11- cis retinal. Comparison of the results from the eyeball extract and the recombinant CrRh strongly suggests that CrRh has an A 1-based 11- cis-retinal chromophore and works as a photoreceptor in the C. rhodomelas retina, and hence that C. rhodomelas responds to dim blue light much the same as other deep-sea fishes. Because hydrothermal vent is a huge supply of viable food, C. rhodomelas likely do not need to participate diel vertical migration and may recognize the bioluminescence produced by aquatic animals living near the hydrothermal vents. 相似文献
6.
Establishing tissue cultures derived from deep-sea multicellular organisms has been extremely difficult because of the serious
damage they sustain upon decompression and exposure to the high temperature of surface seawater. We developed a novel pressure-stat
aquarium system for the study of living deep-sea multicellular organisms under pressure. Using this system, we have succeeded
in maintaining a variety of deep-sea multicellular organisms under pressure and atmospheric conditions after gradual, slow
decompression. Furthermore, we successfully cultivated and freeze-stocked pectoral fin cells of the deep-sea eel Simenchelys parasiticus collected at a depth of 1,162 m under atmospheric pressure conditions. This review describes novel capture and maintenance
devices for deep-sea organisms and cell culture studies of the organisms under atmospheric and pressure conditions. 相似文献
7.
The effects of high hydrostatic pressure on lactate dehydrogenase (LDH) activities from two species of hagfish were examined.
LDH from Eptatretus okinoseanus, a deep-sea species, retained 67% of the original activity even at 100 MPa. LDH activity from Eptatretus burgeri, a shallow-sea species, was completely lost at 50 MPa but recovered to the original value at 0.1 MPa. The tetrameric structure
of LDH-A 4 from E. okinoseanus did not change at 50 MPa. In contrast, almost all LDH tetramers from E. burgeri dissociated to dimers and monomers at 50 MPa but reverted to tetramers at 0.1 MPa. These results show that the dissociation
of tetramers caused the inactivation of E. burgeri LDH. The difference depends on the number 6 and 10 amino acids. The mechanism of the slight, gradual inactivation of E. okinoseanus LDH at high pressure differs and is probably due to the metamorphosis of its inner structures. 相似文献
8.
Behavioral responses of marine and aquatic animals are characterized as R1 (a first response of prolonged excitation), T (tetany or paralysis), I (inactivity and relaxation) and death as measured by the LD 50. R 1, T, and I are reversible responses and temperature dependent with each response occurring at high pressures as temperature increases. The lethal response is also temperature dependent but the tolerance to pressure increases with decreasing temperature. The responses are examined with reference to the ability of animals to successfully cope with the high pressure and low temperature of the abyssal environment. 相似文献
9.
In marine fishes, heterotrimeric guanyl nucleotide binding proteins (G proteins), which couple cell surface membrane receptors to their effector elements, are sensitive to hydrostatic pressure. The intrinsic high affinity GTPase activity of the α subunits of G proteins in three signaling systems coupled to adenylyl cyclase, the A 1 adenosine receptor, the muscarinic cholinergic receptor and the β-adrenergic receptor, was tested at pressures up to 340 atm. Brain membrane preparations from four members of the deep-sea teleost fish family Macrouridae were studied. Coryphaenoides armatus, C. filifer, C. rupestris and Macrourus berglax have depth distributions which together span 100–5810 m. Increased pressure inhibited basal GTPase activity only in M. berglax, which of the four species has the shallowest center of abundance. Increased hydrostatic pressure did not alter the response of GTPase activity to the β-adrenergic receptor agonist isoproterenol. Increased pressure decreased the stimulation of GTPase activity by the A 1 adenosine receptor agonist cyclopentyladenosine (CPA) in C. armatus and M. berglax, and by the muscarinic cholinergic receptor agonist carbamyl choline in C. armatus, C. filifer and M. berglax. Decreased agonist-stimulation of the GTPase activity at elevated pressure may result from pressure-induced changes in conformational states or inhibition of agonist binding. The binding of the non-hydrolyzable GTP analog guanosine 5′-[γ-thio]triphosphate (GTP[S]) in response to CPA was determined at 5 °C and atmospheric pressure. Six macrourid species and a morid were studied. The halftime ( t1/2) values for GTP[S] binding, ranging from 20.8 to 40.9 min, are similar to values previously reported for two other cold-adapted fishes. 相似文献
10.
All biological processes of life on Earth experience varying degrees of pressure. Aquatic organisms living in the deep-sea, as well as chondrocytic cells of articular cartilage are exposed to hydrostatic pressures that rise up to several hundred times that of atmospheric pressure. In the case of marine larvae that disperse through the oceanic water column, pressure changes might be responsible for stress conditions during development, limiting colonisation capabilities. In a number of biological systems, life strategies may be significantly influenced by pressure. In this review, we will focus on the consequences of pressure changes on various biological processes, and more specifically on animals living in the deep-sea. Revisiting general principles of pressure effects on biological systems, we present recent data illustrating the diversity of effects pressure may have at different levels in biological systems, with particular attention to effects on gene expression. After a review of the main pressure equipments available today for studying species living naturally at high pressure, we summarise what is known concerning pressure impact during animal development. 相似文献
11.
We report successful larval hatching of deep-sea shrimp after decompression to atmospheric pressure. Three specimens of deep-sea shrimp were collected from an ocean depth of 1157 m at cold-seep sites off Hatsushima Island in Sagami Bay, Japan, using a pressure-stat aquarium system. Phylogenetic analysis of Alvinocaris sp. based on cytochrome c oxidase subunit gene sequences confirmed that these species were a member of the genus Alvinocaris. All 3 specimens survived to reach atmospheric pressure conditions after stepwise 63-day decompression. Two of the specimens contained eggs, which hatched after 10 and 16 days, respectively, of full decompression. Although no molting of the shrimp larvae was observed during 74 days of rearing under atmospheric pressure, the larvae developed conventional dark-adapted eyes after 15 days. 相似文献
12.
We report on the lens pigmentation and visual pigments of 52 species of demersal deep-sea fishes caught at depths ranging from 480 m to 4110 m in the Porcupine Seabight and Goban Spur area of the North-eastern Atlantic. Only one species, caught between 480 and 840 m, had a lens with large amounts of pigment, consistent with the hypothesis that heavily pigmented lenses in deep-sea fish serve to enhance the contrast of bioluminescent signals by removing much of the background radiance, which is only visible to fish living shallower than 1000 m. Low concentrations of lens pigmentation were also observed in a further two species ( Rouleina attrita and Micromesisteus poutassou). The retinae of all species except five, contained only a single visual pigment, as determined by microspectrophotometry of individual rods, and/or spectrophotometry of retinal wholemounts and retinal extracts. Those fishes caught between 500 m and 1100 m had wavelengths of peak sensitivity ( max) ranging from 476 nm to 494 nm, while most fish living below 1100 m tended to be more conservative with ( max) values ranging from 475 nm to 485 nm. The only exceptions to this were three deep-living species caught between 1600 m and 2000 m whose retinae contain abnormally short-wave sensitive visual pigments ( Cataetyx laticeps — max 468 nm; Alepocephalus bairdii — max 467 nm; Narcetes stomias max 472 nm), suggesting adaptation for the detection of short-wave bioluminescence. 相似文献
13.
A survey of the pressure resistance of aquatic animals in different stages of their life cycle shows that adults generally are more tolerant of pressure than the egg and nauplii, but older adults appear less pressure resistant than younger adults. Data on many species of aquatic animals of different size shows no correlation between size and pressure resistance. It is concluded that size is not a special determinant in the successful deep-sea colonization of shallow-water animals and this is consonant with the fact of occasional large deep-sea species whereas the average size is quite small in comparison with littoral species. 相似文献
14.
The whole-cell protein inventory of the deep-sea barophilic hyperthermophile Thermococcus barophilus was examined by one-dimensional SDS gradient gel electrophoresis when grown under different pressure conditions at 85°C ( T
opt). One protein (P60) with a molecular mass of approximately 60 kDa was prominent at low pressures (0.3 MPa hydrostatic pressure
and 0.1 MPa atmospheric pressure) but not at deep-sea pressures (10, 30, and 40 MPa). About 17 amino acids were sequenced
from the N-terminal end of the protein. Sequence homology analysis in the GenBank database showed that P60 most closely resembled
heat-shock proteins in some sulfur-metabolizing Archaea. A high degree of amino acid identity (81%–93%) to thermosome subunits
in Thermococcales strains was found. Another protein (P35) with molecular mass of approximately 35.5 kDa was induced at 40 MPa hydrostatic
pressure but not under low-pressure conditions. No amino acid sequence homology was found for this protein when the 40 amino
acids from the N-terminal end were compared with homologous regions of proteins from databases. A PTk diagram was generated for T. barophilus. The results suggest that P
habitat is about 35 MPa, which corresponds to the in situ pressure where the strain was obtained.
Received: May 14, 1999 / Accepted: July 30, 1999 相似文献
15.
The current study evaluates the hypothesis, derived from previous investigations, that alterations in dietary fat and cholesterol influence the social behavior of monkeys. Subjects were 62 adult male, cynomolgus macaques ( Macaca fascicularis) assigned originally to an investigation of atherosclerosis regression. This study thus involves a secondary analysis of data derived from an investigation conducted for another purpose. Animals were housed for 14 months' in social groups of five individuals each and initially fed a diet very high in saturated fat and cholesterol to induce coronary artery atherosclerosis. Monkeys were then exposed for 28 months to one of three conditions; (1) a moderately high-fat, high-cholesterol diet and an unstable social environment (in which monkeys were switched among groups monthly); (2) a low-fat, low-cholesterol diet and an unstable social environment; and (3) a low-fat, low-cholesterol diet and a stable social environment. A comparison of animals living in unstable groups revealed that those consuming the low-fat diet exhibited more overt aggression ( P < 0.001) and overt submission ( P < 0.01) than did monkeys eating the high-fat diet. A second comparison involved only those animals living in stable social units. These monkeys, while consuming the low-fat diet, engaged in more aggression and submission ( Ps < 0.05), spent less time in passive body contact or within touching distance ( Ps < 0.001), and spent more time alone ( P < 0.001) than they had initially while consuming a very high-fat diet. The current investigation is the first on this topic to include measures of social behavior in animals both before and after a reduction in dietary fat. The findings that such a reduction is associated with increased agonism and decreased affiliation may help explain the epidemiologic association in human beings between low or reduced plasma cholesterol concentrations and a high incidence of violence-related mortality. More generally, the data are consistent with the hypothesis that there is a negative feedback adaptation providing for appropriate changes in behavior in response to periodic dietary privation. © 1996 Wiley-Liss, Inc. 相似文献
16.
The effects of pressure and temperature on the fatty acid composition in a barotolerant deep-sea bacterium that had branched-chain fatty acids were examined. The major fatty acids of the strain at atmospheric pressure were iso-C 15:0, C 16:1, iso-C 17:0, and iso-C 17:1. As the growth pressure increased, the proportion of unsaturated fatty acid increased because of an increase in the proportion of iso-C 17:1. On the other hand, as the growth temperature decreased, the proportion of unsaturated fatty acid increased because of the increase in the proportion of C 16:1 and C 18:1. 相似文献
17.
We investigated the effect of commercially available enzymes (α-amylase, α-galactosidase, papain, trypsin, and lipase) as
well as proteases from deep-sea bacteria on the larval attachment of the bryozoan Bugula neritina L. The 50% effective concentrations (EC 50) of the commercial proteases were 10 times lower than those of other enzymes. Crude proteases from six deep-sea Pseudoalteromonas species significantly decreased larval attachment at concentrations of 0.03 to 1 mIU ml −1. The EC 50 of the pure protease from the bacterium Pseudoalteromonas issachenkonii UST041101-043 was close to 1 ng ml −1 (0.1 mIU ml −1). The protease and trypsin individually incorporated in a water-soluble paint matrix inhibited biofouling in a field experiment.
There are certain correlations between production of proteases by bacterial films and inhibition of larval attachment. None
of the bacteria with biofilms that induced attachment of B. neritina produced proteolytic enzymes, whereas most of the bacteria that formed inhibitive biofilms produced proteases. Our investigation
demonstrated the potential use of proteolytic enzymes for antifouling defense. 相似文献
18.
Dissimilatory iron-reducing microorganisms play an important role in the biogeochemical cycle of iron and influence iron mineral formation and transformation. However, studies on microbial iron-reducing processes in deep-sea hydrothermal fields are limited. A novel piezophilic, thermophilic, anaerobic, fermentative iron-reducing bacteria of class Clostridia, named Anoxybacter fermentans DY22613 T, was isolated from East Pacific Rise hydrothermal sulfides. In this report, we examined its cell growth, fermentative metabolites, and biomineralization coupled with dissimilatory iron reduction. Both soluble ferric citrate (FC) and solid amorphous Fe(III) oxyhydroxide (FO) could promote cell growth of this strain, accompanied by increased peptone consumption. More acetate, butyrate, and CO 2 were produced than without adding FO or FC in the media. The highest yield of H 2 was observed in the Fe(III)-absent control. Coupled to fermentation, magnetite particles, and iron-sulfur complexes were respectively formed by the strain during FO and FC reduction. Under experimental conditions mimicking the pressure prevailing at the deep-sea habitat of DY22613 T (20?MPa), Fe(III)-reduction rates were enhanced resulting in relatively larger magnetite nanoparticles with more crystal faces. These results implied that the potential role of A. fermentans DY22613 T in situ in deep-sea hydrothermal sediments is coupling iron reduction and mineral transformation to fermentation of biomolecules. This bacterium likely contributes to the complex biogeochemical iron cycling in deep-sea hydrothermal fields. 相似文献
19.
Ophiuroidea is the most speciose of all classes of Echinoderma. It is an important component in benthic ecosystems, occurring in almost all ecological niches of modern seas. To date, the phylogeny and complete evolutionary history of the ophiuroids have not yet been fully resolved. In this study, we sequenced the complete mitochondrial genomes (mitogenomes) of Ophiothrix ( Ophiothrix) exigua and two deep-sea species Histampica sp. CS049 and Ophioplinthaca sp. M5261. These two deep-sea ophiuroids displayed reversed strand-compositional bias and rearranged gene orders. Thirteen distinct patterns of mitochondrial gene order among ophiuroid mitogenomes were detected, with two gene order newly found in Ophiuroidea. Our data supported the gene order found in all sampled Ophiuridae as the most likely ancestral order of all Ophiuroidea. To improve phylogenetic accuracy based on nucleotide differences, two different criteria were used for the analyses: (i) nucleotide sequence from all codon positions (PCG 123); (ii) the NTE method (“Neutral Transitions Excluded”) for ameliorating the misleading effects of a reverse strand bias in the data. The two methods confirmed the polyphyly of the orders Ophiacanthida and Amphilepidia. At family and genus level, Ophiuridae, Ophionotus and Ophioplinthus were not monophyletic. The most notable exception was that the NTE phylogeny showed low variation of branch length. NTE dataset generated younger age for most lower-level nodes than that from PCG123 dataset. All analyses suggested that the ophiuroids radiation occurred around the Permian–Triassic mass extinction event, and the divergence time of the deep-sea lineages was during the Cretaceous. 相似文献
20.
BackgroundTwo deep-sea eels collected from the Western Pacific Ocean are described in this study. Based on their morphological characteristics, the two deep-sea eel specimens were assumed to belong to the cusk-eel family Ophidiidae and the cutthroat eel family Synaphobranchidae. Methods and resultsTo accurately identify the species of the deep-sea eel specimens, we sequenced the mitochondrial genes (cytochrome c oxidase subunit I [COI] and 16S ribosomal RNA [16S rRNA]). Through molecular phylogenetic analysis based on mtDNA COI and 16S rRNA gene sequences, these species clustered with the genera Bassozetus and Synaphobranchus, suggesting that the deep-sea eel specimens collected are two species from the genera Bassozetus and Synaphobranchus in the Western Pacific Ocean, respectively. ConclusionsThis is the first study to report new records of the genera Bassozetus and Synaphobranchus from the Western Pacific Ocean based on COI and 16S rRNA genes 相似文献
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