共查询到20条相似文献,搜索用时 15 毫秒
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Anna R Paolacci Oronzo A Tanzarella Enrico Porceddu Mario Ciaffi 《BMC molecular biology》2009,10(1):11-27
Background
Usually the reference genes used in gene expression analysis have been chosen for their known or suspected housekeeping roles, however the variation observed in most of them hinders their effective use. The assessed lack of validated reference genes emphasizes the importance of a systematic study for their identification. For selecting candidate reference genes we have developed a simple in silico method based on the data publicly available in the wheat databases Unigene and TIGR. 相似文献3.
Katrijn Van Deun Kathleen Marchal Willem J Heiser Kristof Engelen Iven Van Mechelen 《BMC bioinformatics》2007,8(1):181
Background
Microarray compendia profile the expression of genes in a number of experimental conditions. Such data compendia are useful not only to group genes and conditions based on their similarity in overall expression over profiles but also to gain information on more subtle relations between genes and conditions. Getting a clear visual overview of all these patterns in a single easy-to-grasp representation is a useful preliminary analysis step: We propose to use for this purpose an advanced exploratory method, called multidimensional unfolding. 相似文献4.
Jeff W Chou Tong Zhou William K Kaufmann Richard S Paules Pierre R Bushel 《BMC bioinformatics》2007,8(1):427
Background
A common observation in the analysis of gene expression data is that many genes display similarity in their expression patterns and therefore appear to be co-regulated. However, the variation associated with microarray data and the complexity of the experimental designs make the acquisition of co-expressed genes a challenge. We developed a novel method for Extracting microarray gene expression Patterns and Identifying co-expressed Genes, designated as EPIG. The approach utilizes the underlying structure of gene expression data to extract patterns and identify co-expressed genes that are responsive to experimental conditions. 相似文献5.
Background
Expression array data are used to predict biological functions of uncharacterized genes by comparing their expression profiles to those of characterized genes. While biologically plausible, this is both statistically and computationally challenging. Typical approaches are computationally expensive and ignore correlations among expression profiles and functional categories. 相似文献6.
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In search of suitable reference genes for gene expression studies of human renal cell carcinoma by real-time PCR 总被引:1,自引:0,他引:1
Monika Jung Azizbek Ramankulov Jan Roigas Manfred Johannsen Martin Ringsdorf Glen Kristiansen Klaus Jung 《BMC molecular biology》2007,8(1):47
Background
Housekeeping genes are commonly used as endogenous reference genes for the relative quantification of target genes in gene expression studies. No conclusive systematic study comparing the suitability of different candidate reference genes in clear cell renal cell carcinoma has been published to date. To remedy this situation, 10 housekeeping genes for normalizing purposes of RT-PCR measurements already recommended in various studies were examined with regard to their usefulness as reference genes. 相似文献8.
Genome mapping and expression analyses of human intronic noncoding RNAs reveal tissue-specific patterns and enrichment in genes related to regulation of transcription
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Nakaya HI Amaral PP Louro R Lopes A Fachel AA Moreira YB El-Jundi TA da Silva AM Reis EM Verjovski-Almeida S 《Genome biology》2007,8(3):R43
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Background
Normalization of gene expression data refers to the comparison of expression values using reference standards that are consistent across all conditions of an experiment. In PCR studies, genes designated as "housekeeping genes" have been used as internal reference genes under the assumption that their expression is stable and independent of experimental conditions. However, verification of this assumption is rarely performed. Here we assess the use of gene microarray analysis to facilitate selection of internal reference sequences with higher expression stability across experimental conditions than can be expected using traditional selection methods. 相似文献10.
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Global expression analysis of nucleotide binding site-leucine rich repeat-encoding and related genes in Arabidopsis 总被引:1,自引:0,他引:1
Xiaoping Tan Blake C Meyers Alexander Kozik Marilyn AL West Michele Morgante Dina A St Clair Andrew F Bent Richard W Michelmore 《BMC plant biology》2007,7(1):56
Background
Nucleotide binding site-leucine rich repeat (NBS-LRR)-encoding genes comprise the largest class of plant disease resistance genes. The 149 NBS-LRR-encoding genes and the 58 related genes that do not encode LRRs represent approximately 0.8% of all ORFs so far annotated in Arabidopsis ecotype Col-0. Despite their prevalence in the genome and functional importance, there was little information regarding expression of these genes. 相似文献12.
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Background
Normalization of gene expression microarrays carrying thousands of genes is based on assumptions that do not hold for diagnostic microarrays carrying only few genes. Thus, applying standard microarray normalization strategies to diagnostic microarrays causes new normalization problems. 相似文献15.
Background
Oncolytic adenoviruses are promising agents for the multimodal treatment of cancer. However, tumor-selectivity is crucial for their applicability in patients. Recent studies by several groups demonstrated that oncolytic adenoviruses with tumor-/tissue-specific expression of the E1 and E4 genes, which are pivotal for adenoviral replication, have a specificity profile that is superior to viruses that solely target the expression of E1 or E4 genes. Presently the E1 and E4 regions are modified in a time consuming sequential fashion. 相似文献16.
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