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Dominant and specific repression of Xenopus oocyte 5S RNA genes and satellite I DNA by histone H1. 总被引:31,自引:11,他引:20
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A P Wolffe 《The EMBO journal》1989,8(2):527-537
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Transcriptional activation of Xenopus class III genes in chromatin isolated from sperm and somatic nuclei 总被引:8,自引:0,他引:8
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A P Wolffe 《Nucleic acids research》1989,17(2):767-780
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Assembly of transcriptionally active chromatin in Xenopus oocytes requires specific DNA binding factors 总被引:22,自引:0,他引:22
Active minichromosomes assembled on injected 5S RNA gene clones are stable in Xenopus oocytes; endogenous 5S DNA specific factor(s) are required for their assembly. When somatic-type and oocyte-type 5S RNA gene clones are coinjected, the somatic genes are assembled into active minichromosomes, while most of the oocyte genes are assembled into inactive ones. The differential 5S RNA gene expression, which mimics that in somatic cells, appears to result from titration of 5S DNA specific factor(s) by the competing somatic 5S DNA, followed by histone mediated assembly of inactive chromatin on the oocyte 5S DNA. Stable minichromosomes are also assembled on a cloned histone H4 gene; again, intragenic DNA rearrangements affect the efficiency of assembly of active chromatin and differential gene expression occurs after coinjection of two or more H4 DNA constructs. We suggest that the H4 DNA molecules also compete for limiting quantities of specific DNA binding factor(s) required for the assembly of active H4 gene chromatin. 相似文献
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Formation and stability of the 5 S RNA transcription complex 总被引:49,自引:0,他引:49
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Transcription fraction TFIIIC can regulate differential Xenopus 5S RNA gene transcription in vitro 总被引:30,自引:4,他引:26
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A P Wolffe 《The EMBO journal》1988,7(4):1071-1079
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Differential transcription of Xenopus oocyte and somatic-type 5 S genes in a Xenopus oocyte extract 总被引:10,自引:0,他引:10
L Millstein P Eversole-Cire J Blanco J M Gottesfeld 《The Journal of biological chemistry》1987,262(35):17100-17110
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Differential 5S RNA gene expression in vitro 总被引:23,自引:0,他引:23
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Chromosomal footprinting of transcriptionally active and inactive oocyte-type 5S RNA genes of Xenopus laevis 总被引:5,自引:2,他引:3
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The chromatin structure of the Xenopus oocyte-specific 5S rRNA genes was examined at high resolution in immature oocyte and somatic cell chromosomes by DNase I footprinting. On oocyte chromatin, where the genes are active, the cleavage preferences over the entire gene region showed a periodic pattern of sensitivity and were dramatically different from the patterns obtained with deproteinized DNA or somatic cell chromatin. Further, the normal binding site for TFIIIA over the internal promoter region was preferentially sensitive to cleavage, indicating that TFIIIA was not bound in the manner predicted by in vitro experiments. In somatic cell chromatin, the oocyte-type 5S genes displayed a cleavage pattern largely similar to deproteinized DNA suggesting the absence of positioned nucleosomes on these inactive genes, although the presence of uncharacterized repressor complexes could not be ruled out. These data are discussed in terms of potential forms of the chromatin structure and alternative mechanisms of oocyte-type gene activation. 相似文献
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