Genetic rescue can reduce inbreeding depression and increase fitness of small populations, even when the donor populations are highly inbred. In a recent experiment involving two inbred island populations of the New Zealand South Island robin, Petroica australis, reciprocal translocations improved microsatellite diversity and individual fitness. While microsatellite loci may reflect patterns of genome‐wide diversity, they generally do not indicate the specific genetic regions responsible for increased fitness. We tested the effectiveness of this reciprocal translocation for rescuing diversity of two immunogenetic regions: Toll‐like receptor (TLR) and major histocompatibility complex (MHC) genes. We found that the relatively small number of migrants (seven and ten per island) effectively brought the characteristic TLR gene diversity of each source population into the recipient population. However, when migrants transmitted TLR alleles that were already present at high frequency in the recipient population, it was possible for offspring of mixed heritage to have decreased gene diversity compared to recipient population diversity prior to translocation. In contrast to TLRs, we did not observe substantial changes in MHC allelic diversity following translocation, with limited evidence of a decrease in differentiation, perhaps because most MHC alleles were observed at both sites prior to the translocation. Overall, we conclude that small numbers of migrants may successfully restore the diversity of immunogenetic loci with few alleles, but that translocating larger numbers of animals would provide additional opportunity for the genetic rescue of highly polymorphic immunity regions, such as the MHC, even when the source population is inbred. 相似文献
Heterozygosity‐fitness correlations (HFCs) have been observed for several decades, but their causes are often elusive. Tests for identity disequilibrium (ID, correlated heterozygosity between loci) are commonly used to determine if inbreeding depression is a possible cause of HFCs. We used computer simulations to determine how often ID is detected when HFCs are caused by inbreeding depression. We also used ID in conjunction with HFCs to estimate the proportion of variation (r2) in fitness explained by the individual inbreeding coefficient (F). ID was not detected in a large proportion of populations with statistically significant HFCs (sample size = 120 individuals) unless the variance of F was high (σ2(F) ≥ 0.005) or many loci were used (100 microsatellites or 1000 SNPs). For example, with 25 microsatellites, ID was not detected in 49% of populations when HFCs were caused by six lethal equivalents and σ2(F) was typical of vertebrate populations (σ2(F) ≈ 0.002). Estimates of r2 between survival and F based on ID and HFCs were imprecise unless ID was strong and highly statistically significant (P ≈ 0.01). These results suggest that failing to detect ID in HFC studies should not be taken as evidence that inbreeding depression is absent. The number of markers necessary to simultaneously detect HFC and ID depends strongly on σ2(F). Thus the mating system and demography of populations, which influence σ2(F), should be considered when designing HFC studies. ID should be used in conjunction with HFCs to estimate the correlation between fitness and F, because HFCs alone reveal little about the strength of inbreeding depression. 相似文献
Understanding how immune genetic variation is shaped by selective and neutral processes in wild populations is of prime importance in both evolutionary biology and epidemiology. The European roe deer (Capreolus capreolus) has considerably expanded its distribution range these last decades, notably by colonizing agricultural landscapes. This range shift is likely to have led to bottlenecks and increased roe deer exposure to a new range of pathogens that until recently predominantly infected humans and domestic fauna. We therefore investigated the historical and contemporary forces that have shaped variability in a panel of genes involved in innate and acquired immunity in roe deer, including Mhc‐Drb and genes encoding cytokines or toll‐like receptors (TLRs). Together, our results suggest that genetic drift is the main contemporary evolutionary force shaping immunogenetic variation within populations. However, in contrast to the classical view, we found that some innate immune genes involved in micropathogen recognition (e.g. Tlrs) continue to evolve dynamically in roe deer in response to pathogen‐mediated positive selection. Most studied Tlrs (Tlr2, Tlr4 and Tlr5) had similarly high levels of amino acid diversity in the three studied populations including one recently established in southwestern France that showed a clear signature of genetic bottleneck. Tlr2 implicated in the recognition of Gram‐positive bacteria in domestic ungulates, showed strong evidence of balancing selection. The high immunogenetic variation revealed here implies that roe deer are able to cope with a wide spectrum of pathogens and to respond rapidly to emerging infectious diseases. 相似文献
Advances in high‐throughput sequencing have promoted the collection of reference genomes and genome‐wide diversity. However, the assessment of genomic variation among populations has hitherto mainly been surveyed through single‐nucleotide polymorphisms (SNPs) and largely ignored the often major fraction of genomes represented by transposable elements (TEs). Despite accumulating evidence supporting the evolutionary significance of TEs, comprehensive surveys remain scarce. Here, we sequenced the full genomes of 304 individuals of Arabis alpina sampled from four nearby natural populations to genotype SNPs as well as polymorphic long terminal repeat retrotransposons (polymorphic TEs; i.e., presence/absence of TE insertions at specific loci). We identified 291,396 SNPs and 20,548 polymorphic TEs, comparing their contributions to genomic diversity and divergence across populations. Few SNPs were shared among populations and overall showed high population‐specific variation, whereas most polymorphic TEs segregated among populations. The genomic context of these two classes of variants further highlighted candidate adaptive loci having a putative impact on functional genes. In particular, 4.96% of the SNPs were identified as nonsynonymous or affecting start/stop codons. In contrast, 43% of the polymorphic TEs were present next to Arabis genes enriched in functional categories related to the regulation of reproduction and responses to biotic as well as abiotic stresses. This unprecedented data set, mapping variation gained from SNPs and complementary polymorphic TEs within and among populations, will serve as a rich resource for addressing microevolutionary processes shaping genome variation. 相似文献
Genome-wide association studies (GWAS) have become a widely used approach for genetic association studies of various human traits. A few GWAS have been conducted with the goal of identifying novel loci for pigmentation traits, melanoma, and non-melanoma skin cancer. Nevertheless, the phenotype variation explained by the genetic markers identified so far is limited. In this review, we discuss the GWAS study design and its application in pigmentation and skin cancer research. Furthermore, we summarize recent developments in post-GWAS activities such as meta-analysis, pathway analysis, and risk prediction. 相似文献
Show-jumping is an economically important breeding goal in Hanoverian warmblood horses. The aim of this study was a genome-wide association study (GWAS) for quantitative trait loci (QTL) for show-jumping in Hanoverian warmblood horses, employing the Illumina equine SNP50 Beadchip. For our analyses, we genotyped 115 stallions of the National State stud of Lower Saxony. The show-jumping talent of a horse includes style and ability in free-jumping. To control spurious associations based on population stratification, two different mixed linear animal model (MLM) approaches were employed, besides linear models with fixed effects only and adaptive permutations for correcting multiple testing. Population stratification was explained best in the MLM considering Hanoverian, Thoroughbred, Trakehner and Holsteiner genes and the marker identity-by-state relationship matrix. We identified six QTL for show-jumping on horse chromosomes (ECA) 1, 8, 9 and 26 (-log(10) P-value >5) and further putative QTL with -log(10) P-values of 3-5 on ECA1, 3, 11, 17 and 21. Within six QTL regions, we identified human performance-related genes including PAPSS2 on ECA1, MYL2 on ECA8, TRHR on ECA9 and GABPA on ECA26 and within the putative QTL regions NRAP on ECA1, and TBX4 on ECA11. The results of our GWAS suggest that genes involved in muscle structure, development and metabolism are crucial for elite show-jumping performance. Further studies are required to validate these QTL in larger data sets and further horse populations. 相似文献
Phagocytosis provides innate immune cells with a mechanism to take up and destroy pathogenic bacteria, apoptotic cells and other large particles. In some cases, however, peptide antigens from these particles are preserved for presentation in association with major histocompatibility complex (MHC) class I or class II molecules in order to stimulate antigen‐specific T cells. Processing and presentation of antigens from phagosomes presents a number of distinct challenges relative to antigens internalized by other means; while bacterial antigens were among the first discovered to be presented to T cells, analyses of the cellular mechanisms by which peptides from phagocytosed antigens assemble with MHC molecules and by which these complexes are then expressed at the plasma membrane have lagged behind those of conventional model soluble antigens. In this review, we cover recent advances in our understanding of these processes, including the unique cross‐presentation of phagocytosed antigens by MHC class I molecules, and in their control by signaling modalities in phagocytic cells. 相似文献
Captive breeding has the potential to rebuild depressed populations. However, associated genetic changes may decrease restoration success and negatively affect the adaptive potential of the entire population. Thus, approaches that minimize genetic risks should be tested in a comparative framework over multiple generations. Genetic diversity in two captive‐reared lines of a species of conservation interest, Chinook salmon (Oncorhynchus tshawytscha), was surveyed across three generations using genome‐wide approaches. Genetic divergence from the source population was minimal in an integrated line, which implemented managed gene flow by using only naturally‐born adults as captive broodstock, but significant in a segregated line, which bred only captive‐origin individuals. Estimates of effective number of breeders revealed that the rapid divergence observed in the latter was largely attributable to genetic drift. Three independent tests for signatures of adaptive divergence also identified temporal change within the segregated line, possibly indicating domestication selection. The results empirically demonstrate that using managed gene flow for propagating a captive‐reared population reduces genetic divergence over the short term compared to one that relies solely on captive‐origin parents. These findings complement existing studies of captive breeding, which typically focus on a single management strategy and examine the fitness of one or two generations. 相似文献
Incorporating mate choice into conservation breeding programs can improve reproduction and the retention of natural behaviors. However, different types of genetic‐based mate choice can have varied consequences for genetic diversity management. As a result, it is important to examine mechanisms of mate choice in captivity to assess its costs and benefits. Most research in this area has focused on experimental pairing trials; however, this resource‐intensive approach is not always feasible in captive settings and can interfere with other management constraints. We used generalized linear mixed models and permutation approaches to investigate overall breeding success in group‐housed Tasmanian devils at three nonmutually exclusive mate choice hypotheses: (a) advantage of heterozygous individuals, (b) advantage of dissimilar mates, and (c) optimum genetic distance, using both 1,948 genome‐wide SNPs and 12 MHC‐linked microsatellites. The managed devil insurance population is the largest such breeding program in Australia and is known to have high variance in reproductive success. We found that nongenetic factors such as age were the best predictors of breeding success in a competitive breeding scenario, with younger females and older males being more successful. We found no evidence of mate choice under the hypotheses tested. Mate choice varies among species and across environments, so we advocate for more studies in realistic captive management contexts as experimental or wild studies may not apply. Conservation managers must weigh up the need to wait for adequate sample sizes to detect mate choice with the risk that genetic changes may occur during this time in captivity. Our study shows that examining and integrating mate choice into the captive management of species housed in realistic, semi‐natural group‐based contexts may be more difficult than previously considered. 相似文献
We investigated how heterozygosity at the major histocompatibility complex (MHC) affects fitness in wild-derived (F2) house mice (Mus musculus musculus). To compare and control for potential confounding effects from close inbreeding and genome-wide heterozygosity, we used mice that were systematically outbred. We assessed how heterozygosity at MHC and background loci (using 15 microsatellite markers on 11 different chromosomes) affects individual survival and reproductive success (RS) in large, semi-natural population enclosures. We found that overall heterozygosity significantly increased RS, and this correlation was entirely explained by heterozygosity at two MHC loci. Moreover, we found that the effects of MHC heterozygosity depend on the level of background heterozygosity, and the benefits of maximal MHC heterozygosity show a curvilinear effect with increasing background heterozygosity. The enhanced RS from MHC heterozygosity was not because of increased survival, and although MHC heterozygosity was correlated with body mass, body mass did not correlate with RS when heterozygosity is controlled. Breeders were more MHC heterozygous than nonbreeders for both sexes, indicating that MHC heterozygosity enhanced fecundity, mating success or both. Our results show that (i) MHC heterozygosity enhances fitness among wild, outbred as well as congenic laboratory mice; (ii) heterozygosity-fitness correlations can potentially be explained by a few loci, such as MHC; (iii) MHC heterozygosity can increase fitness, even without affecting survival, by increasing mating and RS; and (iv) MHC effects depend on background genes, and maximal MHC heterozygosity is most beneficial at intermediate or optimal levels of background heterozygosity. 相似文献
This report describes single-nucleotide polymorphisms (SNPs) in the sheep major histocompatibility complex (MHC) class II and class III regions and provides insights into the internal structure of this important genomic complex. MHC haplotypes were deduced from sheep family trios based on genotypes from 20 novel SNPs representative of the class II region and 10 previously described SNPs spanning the class III region. All 30 SNPs exhibited Hardy-Weinberg proportions in the sheep population studied. Recombination within an extended sire haplotype was observed within the class II region for 4 of 20 sheep chromosomes, thereby supporting the presence of separated IIa and IIb subregions similar to those present in cattle. SNP heterozygosity varied across the class II and III regions. One segment of the class IIa subregion manifested very low heterozygosity for several SNPs spanning approximately 120 Kbp. This feature corresponds to a subregion within the human MHC class II region previously described as a 'SNP desert' because of its paucity of SNPs. Linkage disequilibrium (LD) was reduced at the junction separating the putative class IIb and IIa subregions and also between the class IIa and the class III subregions. The latter observation is consistent with either an unmapped physical separation at this location or more likely a boundary characterized by more frequent recombination between two conserved subregions, each manifesting high within-block LD. These results identify internal blocks of loci in the sheep MHC, within which recombination is relatively rare. 相似文献
DNA sequencing technology is undergoing a revolution with the commercialization of second generation technologies capable of sequencing thousands of millions of nucleotide bases in each run. The data explosion resulting from this technology is likely to continue to increase with the further development of second generation sequencing and the introduction of third generation single‐molecule sequencing methods over the coming years. The question is no longer whether we can sequence crop genomes which are often large and complex, but how soon can we sequence them? Even cereal genomes such as wheat and barley which were once considered intractable are coming under the spotlight of the new sequencing technologies and an array of new projects and approaches are being established. The increasing availability of DNA sequence information enables the discovery of genes and molecular markers associated with diverse agronomic traits creating new opportunities for crop improvement. However, the challenge remains to convert this mass of data into knowledge that can be applied in crop breeding programs. 相似文献
Adaptation to early training and racing (i.e. precocity), which is highly variable in racing Thoroughbreds, has implications for the selection and training of horses. We hypothesised that precocity in Thoroughbred racehorses is heritable. Age at first sprint training session (work day), age at first race and age at best race were used as phenotypes to quantify precocity. Using high‐density SNP array data, additive SNP heritability () was estimated to be 0.17, 0.14 and 0.17 for the three traits respectively. In genome‐wide association studies (GWAS) for age at first race and age at best race, a 1.98‐Mb region on equine chromosome 18 (ECA18) was identified. The most significant association was with the myostatin (MSTN) g.66493737C>T SNP (P =5.46×10?12 and P =1.89×10?14 respectively). In addition, two SNPs on ECA1 (g.37770220G>A and g.37770305T>C) within the first intron of the serotonin receptor gene HTR7 were significantly associated with age at first race and age at best race. Although no significant associations were identified for age at first work day, the MSTN:g.66493737C>T SNP was among the top 20 SNPs in the GWAS (P =3.98×10?5). Here we have identified variants with potential roles in early adaptation to training. Although there was an overlap in genes associated with precocity and distance aptitude (i.e. MSTN), the HTR7 variants were more strongly associated with precocity than with distance. Because HTR7 is closely related to the HTR1A gene, previously implicated in tractability in young Thoroughbreds, this suggests that behavioural traits may influence precocity. 相似文献
It's a wormy world. All natural vertebrate populations are subject to infection and re‐infection with helminth parasites (Stoll 1947). Even in humans, around one billion people in developing nations are infected by one or several of a range of helminth parasites (Lustigman et al. 2012). Infection by worms is therefore the norm and is reflected in vertebrate immune responses. Thus, there is probably little point in generating an inflammatory response to clear every last worm, with ensuing collateral damage to our own tissue, when rapid re‐infection from the environment by another worm is pretty much assured. Instead, the vertebrate immune system modifies its response to worms, controlling (but not always clearing) these infections and at the same time limiting damage to host tissue caused by inflammatory immune responses (Jackson et al. 2009). The immune system, however, has to fight battles on several fronts and, while fighting a prolonged war of attrition against helminth parasites, it also has to protect against periodic invasion by bacteria, where a rapid response to kill invading microbes before they spread is essential (Fig. 1 ). In this issue of Molecular Ecology, Friberg et al. (2013) ask what effect worm infections have on a host's ability to mount antimicrobial responses. Figure 1 Open in figure viewer PowerPoint Helminths generally produce chronic infections that elicit immune responses characterized by both the activation of T helper type 2 (Th2) cells and the production of regulatory responses, such as the cytokines transforming growth factor beta (TGF‐β) and interleukin‐10 (IL‐10) and regulatory T helper cells (TREG). In combination, this immunological phenotype is often called a ‘modified’ Th2 response. Bacterial infections are recognized by pattern recognition receptors such as the Toll‐like receptors (TLRs) that are able to detect bacterial molecules such as lipopolysaccharides (by TLR‐4), flagellins (by TLR‐5) and unmethylated CpG (by TLR‐9) and generate a rapid inflammatory response [characterized by tumour necrosis factor (TNF)‐α production], particularly at the site of infection. As indicated by the dashed line, these contrasting responses have the potential to interact, especially in animals that spend much of their life harbouring chronic helminth infections that may have systemic anti‐inflammatory effects. Friberg et al. ( 2013 ) begin their study in laboratory mice, where the immunological reagents exist to make mechanistic insights into helminth/bacterial co‐infections in a controlled environment. They focus on Toll‐like receptors (TLRs), which provide a first line of immune defence by recognizing microbial infection, triggering a rapid inflammatory response at the site of infection and can also help develop subsequent, acquired responses (Medzhitov 2001 ). Infection with the nematode Heligmosomoides polygyrus modified these responses but in a counter‐intuitive fashion. One might expect worm infection to reduce TLR‐mediated responses, consistent with a role of chronic helminth infections in down‐regulating inflammatory responses to limit tissue damage. Instead, TLR‐2, TLR‐4 and TLR‐9‐mediated cytokine responses tended to be elevated in worm infections, including that of tumour necrosis factor (TNF)‐α, which is a potent pro‐inflammatory cytokine. Potential explanations are that helminth infection changes the bacterial community composition of the gut flora or that helminth infection damages the gut wall and forces the host to defend itself against gut bacteria. Examining systemic effects of infection for immune responses within a whole animal (as opposed to cell culture) is challenging because immune responses are dynamic and variable. Having given an infective dose of nematodes, both the number of worms and the immune responses to these worms change through time. Friberg et al. ( 2013 ) also find that the dynamics of TLR‐mediated responses differ somewhat depending on the mouse strain used and on whether worm infections are delivered as a single pulse or split across multiple small doses (a so‐called ‘trickle’ infection), which may be more representation of infection in the field (Paterson et al. 2008 ). Varying the mode of infection and the host genetic background is important because the approach taken by much of the immunological literature is to try to eliminate variation by infecting a single host genotype with a single infective dose, which makes it difficult to generalize immunological results to the field. If examining immune responses in laboratory animals is difficult, what are the prospects for examining immune responses in a natural population? From a mechanistic perspective, the prospects would seem hopeless. The (already complex) dynamics of an immune response through time will be compounded by immunological variation among hosts in their pathogen exposure, age, nutrition and so forth that are found in natural populations. However, from an ecological perspective, quantifying the magnitude of variation in TLR‐mediated responses and identifying associations between immune responses and measurable variables such as macroparasite burden help to define what groups of individuals are most vulnerable to bacterial infection (Pedersen & Babayan 2011 ). Friberg et al. ( 2013 ) therefore performed an immuno‐epidemiological study on a natural population of wood mice (Apodemus sylvaticus) and found significant associations between H. polygyrus and ectoparasites on TLR2‐mediated TNF‐α production. The result, however, is a complex one in that the direction of the effect switched sharply between the 2 years in which the population was sampled. The cause of this switch is unclear, but may reflect qualitatively or quantitatively different pathogen exposures between the 2 years. Certainly, these data highlight that immune responses in the field are context‐dependent, even if the nature of that context remains elusive. Friberg et al.'s ( 2013 ) results, and a small but growing body of similar studies (Abolins et al. 2011 ; Boysen et al. 2011 ; Jackson et al. 2011 ), show both the potential and the difficulty in analysing immunological responses in the natural environment. In an earlier review in Molecular Ecology, Pedersen & Babayan ( 2011 ) make a compelling case to study the ecological context of immune responses in the wild. Ultimately, immune responses should enhance individual fitness, but it is not necessarily clear what constitutes a ‘good’ immune response. In particular, individuals in the wild may not have the luxury of deciding whether to tolerate a chronic worm infection or to clear an acute bacterial infection; the prevalence of co‐infection means that often they have to do both, often under conditions of nutritional stress (Pedersen & Babayan 2011 ). We are now only starting to bridge the gap between laboratory immunology and the ecology of natural populations. The technical barriers are readily apparent, including a lack of immunological reagents for non‐model species and the difficulty of sampling individuals and of measuring pathogen infection. Probably, a greater barrier, however, is linguistic; immunologists and ecologists speak very different languages. There is a steep learning curve for any ecologist trying to pick out what immune parameters they should try to measure in their system and how to interpret these. Equally, immunologists are unused to dealing with variation among individuals as anything other than a nuisance. However, there are great rewards for both ecologists and immunologists in understanding the sources of immunological variation in the field. It is therefore to be hoped that a common language can be developed between laboratory immunology and field ecology and that Molecular Ecology will be at the forefront of bringing these two fields together.
References
Abolins S , Pocock M , Hafalla J , Riley EM , Viney ME (2011 ) The immune function of wild mice, Mus musculus . Molecular Ecology, 20 , 881 – 892 . Wiley Online Library CAS PubMed Web of Science® Google Scholar
Boysen P , Eide DM , Storset AK (2011 ) Natural killer cells in free‐living Mus musculus have a primed phenotype . Molecular Ecology, 20 , 5103 – 5110 . Wiley Online Library CAS PubMed Web of Science® Google Scholar
Friberg IM , Little S , Ralli C et al. (2013 ) Macroparasites at peripheral sites of infection are major and dynamic modifiers of systemic anti‐microbial pattern recognition responses . Molecular Ecology, 22 , 2810 – 2826 . Wiley Online Library CAS PubMed Web of Science® Google Scholar
Jackson JA , Friberg IM , Little S , Bradley JE (2009 ) Review series on helminths, immune modulation and the hygiene hypothesis: immunity against helminths and immunological phenomena in modern human populations: coevolutionary legacies? Immunology, 126 , 18 – 27 . Wiley Online Library CAS PubMed Web of Science® Google Scholar
Jackson JA , Begon M , Birtles R et al. (2011 ) The analysis of immunological profiles in wild animals: a case study on immunodynamics in the field vole, Microtus agrestis . Molecular Ecology, 20 , 893 – 909 . Wiley Online Library CAS PubMed Web of Science® Google Scholar
Lustigman S , Prichard RK , Gazzinelli A et al. (2012 ) A research agenda for helminth diseases of humans: the problem of helminthiases . PLoS Neglected Tropical Diseases, 6 , e1582 . Crossref PubMed Web of Science® Google Scholar
Medzhitov R (2001 ) Toll‐like receptors and innate immunity . Nature Reviews Immunology, 1 , 135 – 145 . Crossref CAS PubMed Web of Science® Google Scholar
Paterson S , Wilkes C , Bleay C , Viney ME (2008 ) Immunological responses elicited by different infection regimes with Strongyloides ratti . PLoS ONE, 3 , e2509 . Crossref CAS PubMed Web of Science® Google Scholar
Pedersen AB , Babayan SA (2011 ) Wild immunology . Molecular Ecology, 20 , 872 – 880 . Wiley Online Library CAS PubMed Web of Science® Google Scholar
Stoll NR (1947 ) This wormy world . The Journal of Parasitology, 33 , 1 – 18 . Crossref CAS PubMed Google Scholar
S.P. wrote this article. S.P.‘s research focuses on the causes and consequences of genetic diversity in host and parasite populations. He is a director of the Centre for Genomic Research at the University of Liverpool and applies high‐throughput sequencing and gene expression methods to laboratory, domesticated and natural populations of animals and their pathogens.
Citing Literature
Number of times cited according to CrossRef: 1
M Orsucci, M Navajas, S Fellous, Genotype-specific interactions between parasitic arthropods, Heredity, 10.1038/hdy.2016.90, 118 , 3, (260-265), (2016). Crossref
The Tibetan chicken (TBC), an indigenous chicken breed of the Tibetan Plateau, has adapted to its hypoxic, high‐altitude environment over hundreds of years. The objective of this study was to identify the polymorphisms and genes associated with adaptation to hypoxia in this chicken breed. In the present study, samples were collected during days 18–21 of the incubation period from both surviving chicks and dead embryos, all of which were hatched under hypoxic conditions. A genome‐wide association study was conducted using the Illumina iSelect 60K SNP array with a case–control design, in which the case group consisted of the dead chicken embryos (n =54) and controls were the surviving chicks (n =82). Four significant SNPs were detected at the genome‐wide level (P <0.05), and the results indicated that fork head box G1 (FOXG1) was the main candidate gene. The lead SNP NC_006092.4:g.33368893T>C was confirmed with a polymerase chain reaction‐restriction fragment length polymorphism analysis of 122 cases and 212 controls. A chi‐square test showed a significant association between NC_006092.4:g.33368893T>C and hatchability under hypoxic conditions (P <0.01). Our results revealed novel polymorphisms and a candidate gene associated with hypoxic adaptation, facilitating further study in this field. 相似文献
