共查询到20条相似文献,搜索用时 15 毫秒
1.
Makoto Kashima Kiyokazu Agata Norito Shibata 《Development, growth & differentiation》2020,62(6):407-422
Planarians have a remarkable regenerative ability owing to their adult pluripotent stem cells (aPSCs), which are called “neoblasts.” Planarians maintain a considerable number of neoblasts throughout their adulthood to supply differentiated cells for the maintenance of tissue homeostasis and asexual reproduction (fission followed by regeneration). Thus, planarians serve as a good model to study the regulatory mechanisms of in vivo aPSCs. In asexually reproducing invertebrates, such as sponge, Hydra, and planaria, piwi family genes are the markers most commonly expressed in aPSCs. While piwi family genes are known as guardians against transposable elements in the germline cells of animals that only sexually propagate, their functions in the aPSC system have remained elusive. In this review, we introduce recent knowledge on the PIWI family proteins in the aPSC system in planarians and other organisms and discuss how PIWI family proteins contribute to the regulation of the aPSC system. 相似文献
2.
Tian QN Bao ZX Lu P Qin YF Chen SJ Liang F Mai J Zhao JM Zhu ZY Zhang YZ Zhang ST 《Molecular biology reports》2012,39(3):2653-2658
MicroRNAs (miRNAs) are ~22-nt small non-coding RNAs that regulate the expression of specific target genes in many eukaryotes. miRNAs have been shown to play important roles in stem cell maintenance, cell fate determination, and differentiation. Planarians are capable of regenerating entire body plans from tiny fragments; this regenerative capacity is facilitated by a population of pluripotent stem cells known as neoblasts. Planarians have been a classic model system for the study of many aspects of stem cell biology. However, very limited knowledge on miRNA involved in this regulatory mechanism exists. This study profiles the expression of miRNAs in the normal and regenerative tissues of planarians using miRCURY LNA array technology. Thirteen miRNAs showed significant differences in expression between these two tissues. To further confirm our results, we examined the expression of two miRNAs by qRT-PCR. Results show that some known miRNAs may play key roles in the regulatory mechanisms of regeneration. Our findings can be utilized in future research on miRNA function. 相似文献
3.
Reddien PW 《Trends in genetics : TIG》2011,27(7):277-285
Planarians are flatworms that constitutively maintain adult tissues through cell turnover and can regenerate entire organisms from tiny body fragments. In addition to requiring new cells (from neoblasts), these feats require mechanisms that specify tissue identity in the adult. Crucial roles for Wnt and BMP signaling in the regeneration and maintenance of the body axes have been uncovered, among other regulatory factors. Available data indicate that genes involved in positional identity regulation at key embryonic stages in other animals display persisting regionalized expression in adult planarians. These expression patterns suggest that a constitutively active gene expression map exists for the maintenance of the planarian body. Planarians thus present a fertile ground for the identification of factors regulating the regionalization of the metazoan body plan and for the study of the attributes of these factors that can lead to the maintenance and regeneration of adult tissues. 相似文献
4.
Agata K 《Current opinion in genetics & development》2003,13(5):492-496
Planarians can regenerate using a pluripotent stem cell system. This phenomenon provides a unique opportunity to understand gene regulation in the process of differentiation from pluripotent stem cells. Recent studies have made significant advances in our understanding of the pluripotent stem cell system in this model. In particular, a gene knockdown method by RNA interference enabled great progress in identifying genes involved in regeneration and stem cell regulation. 相似文献
5.
Adult stem cell plasticity: Neoblast repopulation in non-lethally irradiated planarians 总被引:1,自引:0,他引:1
Alessandra Salvetti Leonardo Rossi Annalisa Lena Giuseppe Rainaldi Vittorio Gremigni 《Developmental biology》2009,328(2):305-816
Planarians are a model system for studying adult stem cells, as they possess the neoblasts, a population of pluripotent adult stem cells able to give rise to both somatic and germ cells. Although over the last years several efforts have been made to shed light on neoblast biology, only recent evidence indicate that this population of cells is heterogeneous. In this study we irradiated planarians with different non-lethal X-ray doses (1-5 Gy) and we identified subpopulations of neoblasts with diverse levels of tolerance to X-rays. We demonstrated that a dramatic reduction of neoblasts occurred soon after non-lethal irradiations and that de-novo proliferation of some radioresistant cells re-established the primary neoblast number. In particular, a strong proliferation activity occurred at the ventral side of irradiated animals close to the nervous system. The produced cells migrated towards the dorsal parenchyma and, together with some dorsal radioresistant cells, reconstituted the entire neoblast population demonstrating the extreme plasticity of this adult stem cell system. 相似文献
6.
7.
Sui Huang 《BioEssays : news and reviews in molecular, cellular and developmental biology》2009,31(5):546-560
The stunning possibility of “reprogramming” differentiated somatic cells to express a pluripotent stem cell phenotype (iPS, induced pluripotent stem cell) and the “ground state” character of pluripotency reveal fundamental features of cell fate regulation that lie beyond existing paradigms. The rarity of reprogramming events appears to contradict the robustness with which the unfathomably complex phenotype of stem cells can reliably be generated. This apparent paradox, however, is naturally explained by the rugged “epigenetic landscape” with valleys representing “preprogrammed” attractor states that emerge from the dynamical constraints of the gene regulatory network. This article provides a pedagogical primer to the fundamental principles of gene regulatory networks as integrated dynamic systems and reviews recent insights in gene expression noise and fate determination, thereby offering a formal framework that may help us to understand why cell fate reprogramming events are inherently rare and yet so robust. 相似文献
8.
9.
Hirose H Kato H Kikuchi-Taura A Soma T Taguchi A 《In vitro cellular & developmental biology. Animal》2012,48(3):143-148
Prior to differentiation, embryonic stem (ES) cells in culture are maintained in a so-called “undifferentiated” state, allowing derivation of multiple downstream cell lineages when induced in a directed manner, which in turn grants these cells their “pluripotent” state. The current work is based on a simple observation that the initial culture condition for maintaining mouse ES cells in an “undifferentiated” state does impact on the differentiation propensity of these cells, in this case to a neuronal fate. We point out the importance in judging the “pluripotency” of a given stem cell culture, as this clearly demonstrated that the “undifferentiated” state of these cells is not necessarily a “pluripotent” state, even for a widely used mouse ES cell line. We partly attribute this difference in the initial value of ES cells to the naïve-to-primed status of pluripotency, which in turn may affect early events of the differentiation in vitro. 相似文献
10.
11.
Kazuhiro Sakurada 《Stem cell research》2010,4(3):157-164
The term “environmental epigenetic modifications” refers to alterations in phenotype triggered by environmental stimuli via epigenetic mechanisms. Epidemiologic and animal model studies show that a subset of such environmental epigenetic marks may affect susceptibility to chronic diseases. A growing body of evidence regarding incompleteness of reprogramming indicates that the potential retention of pathogenic environmental epigenetics in human induced pluripotent stem cells (iPSCs) should be seriously considered. Given this possibility, the optimization of methods for the generation of human induc pluripotent stem cells may require the identification of epigenetically appropriate somatic cell sources. Similarly, techniques for controlling epigenetic modification by environmental factors may also play a critical role in the development of epigenetically stable sources of pluripotent stem cells. 相似文献
12.
La s Vicari de Figueiredo Pess a Fabiana Fernandes Bressan Kristine Karla Freude 《World journal of stem cells》2019,11(8):491-505
Up until the mid 2000s, the capacity to generate every cell of an organism was exclusive to embryonic stem cells. In 2006, researchers Takahashi and Yamanaka developed an alternative method of generating embryonic-like stem cells from adult cells, which they coined induced pluripotent stem cells (iPSCs). Such iPSCs possess most of the advantages of embryonic stem cells without the ethical stigma associated with derivation of the latter. The possibility of generating “custom-made” pluripotent cells, ideal for patient-specific disease models, alongside their possible applications in regenerative medicine and reproduction, has drawn a lot of attention to the field with numbers of iPSC studies published growing exponentially. IPSCs have now been generated for a wide variety of species, including but not limited to, mouse, human, primate, wild felines, bovines, equines, birds and rodents, some of which still lack well-established embryonic stem cell lines. The paucity of robust characterization of some of these iPSC lines as well as the residual expression of transgenes involved in the reprogramming process still hampers the use of such cells in species preservation or medical research, underscoring the requirement for further investigations. Here, we provide an extensive overview of iPSC generated from a broad range of animal species including their potential applications and limitations. 相似文献
13.
Characterization and categorization of fluorescence activated cell sorted planarian stem cells by ultrastructural analysis 总被引:1,自引:0,他引:1
Higuchi S Hayashi T Hori I Shibata N Sakamoto H Agata K 《Development, growth & differentiation》2007,49(7):571-581
Planarians have regenerative ability made possible by pluripotent stem cells referred to as neoblasts. Classical ultrastructural studies have indicated that stem cells can be distinguished by a unique cytoplasmic structure known as the chromatoid body and their undifferentiated features, and they are specifically eliminated by X-ray irradiation. Recently, by using fluorescence activated cell sorting (FACS), planarian cells were separated into two X-ray-sensitive fractions (X1 and X2) and an X-ray-insensitive fraction (XIS) according to DNA content and cytoplasmic size. Here we analyzed the fractionated cells by transmission electron microscopy (TEM). First, we found that both undifferentiated cells (stem cells) and regenerative cells (differentiating cells) were concentrated in the X1 fraction containing the S/G2/M phase cells. The regenerative cells were considered to be committed stem cells or progenitor cells, suggesting that some stem cells may maintain proliferative ability even after cell fate-commitment. Second, we succeeded in identifying a new type of stem cells, which were small in size with few chromatoid bodies and a heterochromatin-rich nucleus. Interestingly, they were concentrated in the X2 fraction, containing G0/G1 phase cells. These results suggest that planarian stem cells are not homogeneous, but may consist of heterogeneous populations, like mammalian stem cells. 相似文献
14.
Richard Milne 《New genetics and society》2016,35(4):393-408
This paper explores the promise of induced pluripotent stem cells as a model system for the study of neurodegenerative diseases of Alzheimer’s, Parkinson’s and other diseases of the aging brain. Research in these areas, as in neuroscience more broadly, has struggled with the imperfect mapping between human and animal brains. The paper argues that the contemporary promise of induced pluripotent stem cells for research is established through their potential to resolve problems of translation, bridging laboratory and clinical contexts by acting as a model of “real” patient bodies. However, the paper shows how this promise is contested and renewed through a rearticulation of the relationship between neurodegeneration, aging and the qualities of “young” and “aged” bodies. This not only results in the introduction of new qualities and attributes to the model system, but also a re-imagining of how aging features within both late and early-onset neurological diseases. 相似文献
15.
Generation of chimeric rhesus monkeys 总被引:1,自引:0,他引:1
Totipotent cells in early embryos are progenitors of all stem cells and are capable of developing into a whole organism, including extraembryonic tissues such as placenta. Pluripotent cells in the inner cell mass (ICM) are the descendants of totipotent cells and can differentiate into any cell type of a body except extraembryonic tissues. The ability to contribute to chimeric animals upon reintroduction into host embryos is the key feature of murine totipotent and pluripotent cells. Here, we demonstrate that rhesus monkey embryonic stem cells (ESCs) and isolated ICMs fail to incorporate into host embryos and develop into chimeras. However, chimeric offspring were produced following aggregation of totipotent cells of the four-cell embryos. These results provide insights into the species-specific nature of primate embryos and suggest that a chimera assay using pluripotent cells may not be feasible. 相似文献
16.
17.
MicroRNAs (miRNAs) are approximately 22-nt RNA molecules that typically bind to the 3' untranslated regions of target mRNAs and function to either induce mRNA degradation or repress translation. miRNAs have been shown to play important roles in the function of stem cells and cell lineage decisions in a variety of organisms, including humans. Planarians are bilaterally symmetric metazoans that have the unique ability to completely regenerate lost tissues or organs. This regenerative capacity is facilitated by a population of stem cells known as neoblasts. Planarians are therefore an excellent model system for studying many aspects of stem cell biology. Here we report the cloning and initial characterization of 71 miRNAs from the planarian Schmidtea mediterranea. While several of the S. mediterranea miRNAs are members of miRNA families identified in other species, we also identified a number of planarian-specific miRNAs. This work lays the foundation for functional studies aimed at addressing the role of these miRNAs in regeneration, cell lineage decisions, and basic stem cell biology. 相似文献
18.
19.
The morphology and growth of a pluripotent teratocarcinoma cell line and its derivatives in tissue culture 总被引:5,自引:0,他引:5
Cultures of the clonally derived pluripotent teratocarcinoma cell line, SIKR, are heterogeneous. They are characterized by the presence of two cell types—the “C cells”, which grow as tight, round colonies on a monolayer of the morphologically distinct “E cells”. In contrast to the C cells, whose proliferation is apparently uninhibited by high cell density, the E cells show density-dependent inhibition of growth. Subclones of SIKR are of two types: they are either similar to the parent culture, in that they contain both C and E cells (CE subclones) and are themselves tumorigenic and pluripotent; or they are composed only of E cells (E-type subclones), which are primarily not tumorigenic, but may become so after spontaneous transformation in vitro. The tumors formed by transformed E cells (E-t cells) are monotypic (“fibroblastic”), consisting of one cell type which is not clearly identifiable, but which is distinctly not embryonal carcinoma.It is concluded that the tumorigenic C cells are the stem cells of this teratocarcinoma line, and that they give rise to nontumorigenic E cells in vitro, but that the reverse does not occur. It is suggested that the C to E transition represents cell determination in vitro. The interest of this cell culture system for both developmental and oncological studies is discussed. 相似文献
20.
Young HE Duplaa C Romero-Ramos M Chesselet MF Vourc'h P Yost MJ Ericson K Terracio L Asahara T Masuda H Tamura-Ninomiya S Detmer K Bray RA Steele TA Hixson D el-Kalay M Tobin BW Russ RD Horst MN Floyd JA Henson NL Hawkins KC Groom J Parikh A Blake L Bland LJ Thompson AJ Kirincich A Moreau C Hudson J Bowyer FP Lin TJ Black AC 《Cell biochemistry and biophysics》2004,40(1):1-80
Tissue restoration is the process whereby multiple damaged cell types are replaced to restore the histoarchitecture and function
to the tissue. Several theories, have been proposed to explain the phenomenon of tissue restoration in amphibians and in animals
belonging to higher order. These theories include dedifferentiation of damaged tissues, transdifferentiation of lineage-committed
progenitor cells, and activation of reserve, precursor cells. Studies by Young et al. and others demonstrated that connective
tissue compartments throughout postnatal individuals contain reserve precursor cells. Subsequent repetitive single cell-cloning
and cell-sorting studies revealed that these reserve precursor cells consisted of multiple populations of cells, including,
tissue-specific progenitor cells, germ-layer lineage stem cells, and pluripotent stem cells. Tissue-specific progenitor cells
display various capacities for differentiation, ranging from unipotency (forming a single cell type) to multipotency (forming
multiple cell types). However, all progenitor cells demonstrate a finite life span of 50 to 70 population doublings before
programmed cell senescence and cell death occurs. Germ-layer lineage stem cells can form a wider range of cell types than
a progenitor cell. An individual germ-layer lineage stem cell can form all cells types within its respective germ-layer lineage
(i.e., ectoderm, mesoderm, or endoderm). Pluripotent stem cells can form a wider range of cell types than a single germ-layer
lineage stem cell. A single pluripotent stem cell can form cells belonging to all three germ layer lineages. Both germ-layer
lineage stem cells and pluripotent stem cells exhibit extended capabilities for self-renewal, far surpassing the limited life
span of progenitor cells (50–70 population doublings). The authors propose that the activation of quiescent tissue-specific
progenitor cells, germ-layer lineage stem cells, and/or pluripotent stem cells may be a potential explanation, along with
dedifferentiation and transdifferentiation, for the process of tissue restoration. Several model systems are currently being
investigated to determine the possibilities of using these adult quiescent reserve precursor cells for tissue engineering. 相似文献