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1.
母猪卵泡发育及排卵诱导 总被引:3,自引:0,他引:3
PMSG was used to study the follicular development and ovulation regularity of the sows.The results showed that the earlier copulation caused better ovulation effect after sows were injected PMSG,and the ovulation time of estrous sows centralized 12~63.5 h after accepting copulation.The observation of early embryos morphology showed that the embryos in the oviduct were in 2~4 cell stage,when entered womb,they were in 4~8 cell stage during 51~59.5 h and all of them entered womb during 71~83.5 h after the sows accepted copulation. 相似文献
2.
Anna Gumieniczek Hanna Hopkała Jolanta Rzymowska Maciej Niemczyk 《Central European Journal of Biology》2006,1(3):333-344
In the present study, DNA oxidative damage was elevated and superoxide dismutase (Cu,Zn-SOD) metabolism was disturbed in the
kidney of alloxan-induced diabetic animals. The effects of pioglitazone and repaglinide, new oral antidiabetics, on 8-hydroxy-2′-deoxyguanosine
(8-OHdG) and Cu,Zn-SOD were studied. Diabetic versus control levels (mean ± SE) of 8-OHdG were 24.9 ± 0.2 vs. 21.8 ± 0.1 and
21.5 ± 0.2 vs 20.1 ± 0.2 pmol/μg DNA after 4 and 8 weeks, respectively. At p<0.05, pioglitazone diminished this parameter
in diabetic animals (22.0 ± 0.2 and 20.1 ± 0.3 pmol/μg DNA). The level was not affected in diabetic groups receiving repaglinide
(24.9 ± 0.2 and 21.5 ± 0.3 pmol/μg DNA). In diabetic kidney, Cu,Zn-SOD mRNA was diminished relative to control animals and
was modulated by pioglitazone and repaglinide treatments. Simultaneously, Cu,Zn-SOD activity was also diminished (1.5 ± 0.2
vs. 2.8 ± 0.3 and 1.8 ± 0.1 vs 2.9 ± 0.3 U/mg protein after 4 and 8 weeks, respectively) and partly changed after pioglitazone
(2.1 ± 0.4 and 2.3 ± 0.3 U/mg protein) and repaglinide (2.0 ± 0.1 and 2.4 ± 0.2 U/mg protein). These results suggest that
a reduction in oxidative stress in diabetic kidney can be achieved with the administration of pioglitazone and to some extent
using repaglinide treatment. 相似文献
3.
The daily change in both percentage and absolute number of circulating major lymphocyte subset was determined with young Japanese
monkeys and rhesus monkeys. The blood sample was collected at four hour-intervals beginning at 16:00 for 24 hours under the
condition of applying tethering system by which blood samples could be collected without restraint. During the dark period
(from 20:00 to 08:00), the number of peripheral lymphocytes increased and that of granulocytes decreased, resulting in no
significant change in the number of total peripheral white blood cells. The absolute number of CD4 + T, CD8 + T, and CD20
+ B cells showed the significant daily change similar to that in number of peripheral lymphocytes, indicating no proportional
change in these subsets. The typical proportional change was observed in CD16 + natural killer (NK) cells and the percentage
of CD16 + cells decreased during dark period (from 20:00 to 04:00) and increased in the morning (from 08:00 to 12:00). The
NK activity determined by killing K562 target cells showed the same changing pattern as that of percentage in CD16+ NK cells.
The changing pattern of both percentage and activity of NK cells was consistent with that of plasma cortisol levels. In addition,
the intravenous injection of 300 μg/kg of cortisol induced increase in plasma cortisol levels and decrease in percentage of
CD16 + NK cells during the first 60 min after cortisol injection. These results strongly suggest that the levels of peripheral
functional CD16 + NK cells might be directly regulated by plasma cortisol level in macaque monkeys. 相似文献
4.
Jian-Xun TANG Jun-Rong LI Zhong-Liang LIU Hua ZHAO Xiao-Min TAO Zhang-Shun CHENG 《动物学研究》2013,(5):135-139
This study compared the accumulation of Zn2+and Cu2+in the ovaries and ova of loaches under different concentrations of Zn2+(1.00, 2.50 and 5.00 mg/L respectively) and Cu2+(0.10, 0.25 and 0.50 mg/L respectively). The results showed that both Zn2+and Cu2+accumulated in the ovaries, and that the relationship between accumulation and time was linear over 20 days of exposure. The accumulation of the metals in ovaries was closely related to the concentration of exposure in the solutions(P<0.05), and was obviously affected by the time and doses. However, the Cu2+concentration was significantly higher than Zn2+(P<0.05). The development level of ova in the ovaries also correlated with the concentration and exposure period in the Zn2+and Cu2+solutions. 相似文献
5.
ZHANG WANHUI YUNLONG ZHU FUZHOU WANG YUZHEN HU QI MEI CHAO ZHAO JIANGKANG CHEN 《Cell research》1995,5(2):197-207
The undecapeptide substanceP(SP) was shown to be intimately involved in both the structural and functional aspects of the anterior pituitary.Yet,in addition to its influences on hormonal secretion,SP may well possess more actions in this master gland.The present study was ftherefore aimed to investigate the effect of SP on the proliferation of rat anterior pituitary cells in primary culture,It was found that SP could dose-dependently increase the incorporation of tritiated thymidine(3H-TdR) into cultured anterior pituitary cells.Other mammalian tachykinins such as neurokinin A and neurokinin B had similar effect but to varying degrees.The equipotent analogue of SP,Norleucine^11-SP(Nle^11-SP),also acted likewise.with its action antagonizable by spantide,a SP receptor blocker.To further characterize the nature of cells responsive to the challenge of SP,immunocytochemical staining against S-100 protein and some adenohypophyseal hormones was performed alone or plus autoradiography.The results showed that the percentage of S-100 proteinimmunorective cells was apparently elevated by the addtion of Nle^11-Sp for 48h,which indicates a preferential proliferation of folliculo-stellate cells under the regime .This was confirmed by increases in immunocytochemical or autoradiographical labelling indices of anterior pituitary Substance P and anterior pituitary cell proliferation.Cells treated similarly.Taken together,These results reveal that the trophic action of SP observed previously in other tissues is also present at least in cultured rat anterior pituitary cells.with responding cells being predominantly folliculo-stellate cells as typified by S-100 proteinimmunoreactivity.Therefore,an intra-pituitary trophicaction of SP in vivo could be anticipated. 相似文献
6.
Monoclonal antibody (MAb) attained from hybridoma 1A5 was against human cardiac myosin heavy chains (CMHC) and didn't react with smooth muscle. It was labeled with Na 125 I and injected to the rats suffered from acute myocardial necrosis, which were treated by isoproylarterenol. After injection, the rats were studied from the 4 th to the 48 th hours by relative radioactivity in blood, thyroid gland, stomach, liver, lung, muscle and heart, respectively. The results indicated that 125 I CMHC MAb could specifically reach the target necrotic organ in the 4 th hours (2 03±0 60 ) and remained up to the 48 th hours (2 55±0 49) or longer, at which the 8 th was higher (4 89±0 44). Meanwhile 125 I CMHC MAb showed much higher affinity to necrotic cardiac tissue than the normal ( P <0 001). The same results were should in the blood from 4 th (4 94±0 45) to 48 th (2 70±0 12). Thus 125 I CMHC MAb can be considered as an effective tool for image diagnosis of acute myocardial necrosis. 相似文献
7.
Alfthan G Xu GL Tan WH Aro A Wu J Yang YX Liang WS Xue WL Kong LH 《Biological trace element research》2000,73(2):113-125
Keshan disease is a cardiomyopathy restricted to the endemic areas of China and seen in residents having an extremely low
selenium (Se) status. Prophylactic administration of sodium selenite has been shown to decrease significantly the incidence
of acute and subacute cases. The aim of the study was to assess the relative bioavailability of selenite versus organic Se-yeast
in a Se-deficient area in China with a randomized double-blind double-dummy design.
Healthy children (n=30) between 14 and 16 yr of age were randomized into three equal groups receiving either 200 μg/d selenite Se or 200 μg/d
Se-yeast or placebo for 12 wk. Blood was drawn at baseline, 4, 8, and 12 wk and 4 wk postsupplementation. The plasma Se concentration
(mean ± SD) was 0.16±0.03 μmol/L at baseline. Selenite and Se-yeast supplementation increased plasma Se to plateau values,
1.0±0.2 and 1.3±0.2 μmol/L, respectively. In red cells, Se-yeast increased the selenium level sixfold and selenite threefold
compared to placebo. The relative bioavailability of Se-yeast versus selenite measured as glutathione peroxidase (GSHPx) activity
was similar in plasma, red blood cells, and platelets. GSHPx activity reached maximal levels in plasma and platelets of 300%
and 200%, respectively, after 8 wk compared to the placebo group, but continued to increase in red cells for 16 wk.
Our study showed that although both forms of Se were equally effective in raising GSHPx activity, Se-yeast provided a longer
lasting body pool of Se. Se-yeast may be a better alternative to selenite in the prophylaxis of Keshan disease with respect
to building up of body stores. 相似文献
8.
Total aluminum, chromium, copper, iron, manganese, and nickel were determined in black tea, green tea, Hibiscus sabdariffa, and Ilex paraguariensis (mate) by electrothermal atomic absorption spectrometry after nitric/perchloric acid digestion. In each case, one ground
sample of commercially available leafy material was prepared and three 0.5-g subsamples were run in parallel. The infusions
were also analyzed and the percentage of each element leached into the liquor was evaluated. The obtained results indicated
that hibiscus and mate contained lower levels of aluminum (272±19 μg/g and 369±22 μg/g, respectively) as referred to black
tea (759±31 μg/g) or green tea (919±29 μg/g) and suggested that mate drinking could be a good dietary source of essential
micronutrient manganese (total content 2223±110 μg/g, 48.1% leached to the infusion). It was also found that the infusion
of hibiscus could supply greater amounts of iron (111±5 μg/g total, 40.5% leached) and copper (5.9±0.3 μg/g total, 93.4% leached)
as compared to other infusions. Moreover, it was found that the percentage of element leached to the infusion was strongly
related to the tannins content in the beverage (correlation coefficients >0.82 with the exception for nickel); for lower tannins
level, better leaching was observed. 相似文献
9.
Female-specific serum protein(FSSP)is normally present in the sera of female fish,but it is notfound in males.However,estradiol benzoate(EB)was found to induce the appearance of FSSP inmale fish and immature female fish.Massive doses of FSSP caused a rapid increase in FSSP.Ultrastructural examination of the liver indicated an extensive proliferation of the rough endoplasmicreticulum and a decrease in glycogen and lipid droplets after EB injection.A preparative PAGE for isolating highly purified FSSP from the serum of EB-treated fish,Carassiusauratus cuvieri(Temminck & Schlegel),is described.Purified FSSP obtained from EB-induced O-crucian fish has a molecular weight of 466,000±4,000(n=10),while that in mature female serumis 480,000±40,000(n=2).FSSP appears to be a dimer,with the size of the possible monomerbeing 240,000±8,000(n=6).SDS-PAGE on gradient gels indicated that sera from males given multiple(12)injections of EBcontain a main band with a molecular weight of 147,000±6,000(n=6).However,the same serumsamples provided three bands of protein on the PAGE gels.Antiserum was raised against the electrophoretically purified FSSP.The resulting antibody formeda single,continuous precipitation line with sera from EB-treated males and vitellogenic females,butnot with that from normal males.lmmunocytochemistry(PAP method)was used to locate FSSP in the liver and ovary of maturefemales and the liver of EB-treated males.Strongly positive particles were found clustered in groupsaround liver cell nuclei under light microscopy,and the yolk granules in the oocytes were also filledwith positive particles. 相似文献
10.
人重组γ-干扰素对兔妊娠的影响 总被引:7,自引:1,他引:7
ao et al. (1999) reported that human recombinet interferon gamma (hrIFN γ) exerted a certain anti contraceptive effect on the pregnant rabbit. In order to investigate the possible mechanisms by which interferon gamma (IFN γ) exerts its deleterious effect on pregnancy, 12 New Zealand breed rabbits were used as an animal model. One day (Day 1) after the females were mated with male animals, They were randomly divided into 3 groups. There were control, hrIFN γ 50 000 IU and hrIFN γ 100 000 IU groups. Saline and hrIFN γ of different doses were administered respectively to control and experimental rabbits via vaginal muscular injection on Day 6 and were killed on Day 12 of pregnancy. The fetus and placenta were weighed. The blood was collected before injection and at various intervals(6, 12, 24, 48 and 96 h) after injection. The concentration of serum progesterone were measured by radioimmunoassay. The apoptosis in placenta were examined by DNA fragmentation analysis. The results were as folloows: 1) In control, 50 000 IU and 100 000 IU hrIFN γ groups, progesterone level in serum were 26 20±0 74 ng/ml,17 81±0 55 ng/ml and 10 97±0 84 ng/ml respectively at 96 h after injection. In contrast with control group, progesterone production dropped significantly in rabbits treated with hrIFN γ, especially 100 000 IU hrIFN γ. 2) Apoptotic fragmentation of DNA(180 bp units) in placenta were detected both in control and experimental groups. The scan density of degraded DNA fragmentation in experimental group was higher than that in control, which suggested that apoptosis in placenta was further induced by hrIFN γ, especially by high dose hrIFN γ. In addition, the weight of placenta in rabbits treated with hrIFN γ reduced significantly as compared with that in control group( P <0 01). We suggest that hrIFN γ inhibits the secretion of progesterone, as a result of inducing apoptosis in placenta. 相似文献
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Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis
It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis. 相似文献
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RICHARD E. NORRIS 《Botanical journal of the Linnean Society. Linnean Society of London》1991,106(1):1-40
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera. 相似文献
18.
JOAN VALUÈS MONTSERRAT TORRELL NÚRIA GARCIA JACAS 《Botanical journal of the Linnean Society. Linnean Society of London》2001,137(4):399-407
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted. 相似文献
19.
肝癌中HBV和HCV基因和抗原的分布及意义 总被引:1,自引:0,他引:1
采用原位分子杂交方法检测HCV
RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV
RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV
RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV
X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV
C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细 相似文献
20.
H.-R. GREGORIUS 《Biological journal of the Linnean Society. Linnean Society of London》1984,23(2-3):157-165
For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献