阴道毛滴虫染色体组型观察

采用火焰干燥法制备阴道毛滴虫染色体标本,按 Levan等方法,进行核型分析。阴道毛滴虫的染色体数目为n＝10,2n ＝20,染色体的组型为3m、3sm、1st和3T。 Abstract：　The chromosomes ofTrichomonas vaginaliswere prepared by flame-drying method. Data of chromosome were measured and calculated. According to Levan's method, analysis of karyotype was described. The results showed that the chromosome number ofT. vaginalisconsisted of twenty pairs(2n=20) and chromosome type was 3m, 3sm, 1st and 3T.     

阴道毛滴虫染色体组型观察

采用火焰干燥法制备阴道毛滴虫染色体标本,按 Levan等方法,进行核型分析。阴道毛滴虫的染色体数目为n＝10,2n ＝20,染色体的组型为3m、3sm、1st和3T。 Abstract：　The chromosomes ofTrichomonas vaginaliswere prepared by flame-drying method. Data of chromosome were measured and calculated. According to Levan's method, analysis of karyotype was described. The results showed that the chromosome number ofT. vaginalisconsisted of twenty pairs(2n=20) and chromosome type was 3m, 3sm, 1st and 3T.     

Effect of iron on the virulence of Trichomonas vaginalis

The role of iron was evaluated with respect to the virulence of Trichomonas vaginalis in mice. Iron-supplemented and iron-depleted Diamond's trypticase-yeast extract-maltose (TYM) media were prepared by adding 360 microM of ferrous sulfate and 100 microM of 2,2'-dipyridyl. Trophozoites cultivated from normal TYM and iron-supplemented TYM media produced subcutaneous abscesses; however, trichomonads grown in an iron-deficient TYM medium failed to produce any pathology. In addition to the increased virulence of trophozoites in mice, iron affects the level of adherence and the cytotoxicity of trichomonads to HeLa cells, which are significantly reduced in trophozoites grown in iron-deficient medium. In conclusion, it is suggested that under iron-depleted conditions such as that induced by 2,2'-dipyridyl the virulence of T. vaginalis is reduced.     

Genetic identity and differential gene expression between Trichomonas vaginalis and Trichomonas tenax

Background  

Trichomonas vaginalis is a human urogenital pathogen responsible for trichomonosis, the number-one, non-viral sexually transmitted disease (STD) worldwide, while T. tenax is a commensal of the human oral cavity, found particularly in patients with poor oral hygiene and advanced periodontal disease. The extent of genetic identity between T. vaginalis and its oral commensal counterpart is unknown.     

The effect of hormones on Trichomonas vaginalis

The hormonal milieu can alter susceptibility to infection. The effect of hormones on Trichomonas vaginalis was studied utilizing axenically cultured clinical isolates. Oestrogens, in physiological concentrations, decreased the growth of the organisms and their attachment to mammalian cells in vitro, and acted as a chemorepellent. The specificity of these effects was verified by their being blocked with anti-oestrogens, by the dose- and time-dependency of the responses, and by the lack of effect with other hormones. These results suggest that oestrogens may decrease the virulence of T. vaginalis; however, interactions between oestrogens and mammalian cells may promote the development of infection. Thus complicated interactions between hormones, micro-organisms and mammalian cells must determine whether exposure to oestrogens predisposes to or prevents the development of infection.