Cover Image Melocactus sergipensis N.P. Taylor & M.V. Meiado (Cactaceae) at the holotype locality with limestone substrate in an area of Caatinga, Simão Dias municipality, Sergipe, Brazil. Photo taken by Marcos V. Meiado.
In azoospermic patients, spermatozoa are routinely obtained by testicular sperm extraction (TESE). However, success rates of this technique are moderate, because the site of excision of testicular tissue is determined arbitrarily. Therefore the aim of this study was to establish probe‐based laser endomicroscopy (pCLE) a noval biomedical imaging technique, which provides the opportunity of non‐invasive, real‐time visualisation of tissue at histological resolution. Using pCLE we clearly visualized longitudinal and horizontal views of the tubuli seminiferi contorti and localized vital spermatozoa. Obtained images and real‐time videos were subsequently compared with confocal laser scanning microscopy (CLSM) of spermatozoa and tissues, respectively.
Comparative visualization of single native Confocal laser scanning microscopy (CLSM, left) and probe‐based laser endomicroscopy (pCLE, right) using Pro FlexTM UltraMini O after staining with acriflavine. 相似文献
Mechanisms of renal autoregulation generate oscillations in arterial blood flow at several characteristic frequencies. Full‐field laser speckle flowmetry provides a real‐time imaging of superficial blood microcirculation. The possibility to detect changes in oscillatory dynamics is an important issue in biomedical applications. In this paper we show how laser power density affects quality of the recorded signal and improves detectability of temporal changes in microvascular perfusion.
Cover Image Left: Papaver fauriei growing only on the gravelly alpine slopes of Mt. Rishiri. Right: Cultivated poppies (Papaver sp.) found in seaside towns on Rishiri Island. Photographed by Kazuki Kosugi and Tetsuya Kondo. Rishiri Island, Hokkaido, Japan
Cover Image Top left: Natural hybrids of Yezo spruce (Picea jezoensis var. jezoensis) and Sakhalin spruce (Picea glehnii) occur at low frequencies in this forest around bog in The University of Tokyo Hokkaido Forest, Furano, Hokkaido. Top right: Current‐year shoot of Yezo spruce (left), Sakhalin spruce (right), and their natural hybrid (center). Bottom left: A branch with ripe cones of Sakhalin spruce in Ochiishi, Nemuro, Hokkaido. Bottom right: A branch with ripe cones of Yezo spruce in the Uryu Experimental Forest, Hokkaido University, Horokanai, Hokkaido. All photos taken by Mineaki Aizawa.
Cover Image Left: Flowering individuals of Gastrodia elata. Right: Individual flowers of Gastroidia elate. Photographed by Naoto Sugiura. Rebun Island, Hokkaido, Japan
Cover Image A seed predator Sibinia weevil visiting a flower of Dianthus superbus var. longicalycinus. Photographed by Takashi Miyake. Araihama Forest Park, Niigata, Japan.
Transformation of Ulva partita (Photos by R. Suzuki). Mitochondria in the 2‐cell phase are visualized using green fluorescent protein (GFP). Left: Chlorophyll autofluorescence (red). Middle: GFP fluorescence (green). Right: Merged image of GFP and chlorophyll fluorescence. See Suzuki et al. See pages 176–184. Cover picture from: Article link here
A carageenophyte, Eucheuma denticulatum (Solieriaceae, Rhodophyta) at a mariculture farm in Bali, Indonesia (Photo by R. Terada). Characteristic thermal and irradiance responses on the photosynthesis of Indonesian E. denticulatum and Kappaphycus alvarezii are reported by Borlongan et al. in this issue. Cover picture from: Article link here
Over the past years it had been demonstrated that multimodal imaging combining the nonlinear modalities coherent anti‐Stokes Raman scattering (CARS), two‐photon excited auto‐fluorescence (TPEF) and second harmonic generation (SHG) show a great potential for tissue diagnosis and tumor identification. To extend the applicability of this multimodal imaging approach for in‐vivo tissue screening of difficult to access body regions the development of suitable fiber optic probes is required. Here we report about a novel CARS imaging fiber probe consisting of 10,000 coherent light guiding elements preserving the spatial relationship between the entrance and the output of the fiber. Therefore the scanning procedure can be shifted from the distal to the proximal end of the fiber probe and no moving parts or driving current are required to realize in‐vivo CARS endoscopy.
Back scattered CARS image of rabbit aorta with plaques (white) using a laser scanning microscope and an imaging fiber. 相似文献
Primary segregative cell division in Struvea okamurae (Photos by A. Hasebe). The same apical portion of a cylindrical cell was photographed in order from left to right 72 min, 109 min, 126 min, 136 min, 148 min, 294 min, and 492 min after the light period had begun. See Okuda et al. See pages 219–229. Cover picture from: Article link here
The paper presents problems and solutions related to hyperspectral image pre‐processing. New methods of preliminary image analysis are proposed. The paper shows problems occurring in Matlab when trying to analyse this type of images. Moreover, new methods are discussed which provide the source code in Matlab that can be used in practice without any licensing restrictions.
The proposed application and sample result of hyperspectral image analysis. 相似文献
Cover Image Left: Close‐up photograph of a hairy plant of Arabidopsis halleri subsp. gemmifera. Right: Larva of Phaedon brassicae feeding on a glabrous plant. Photographs by Yasuhiro Sato. Locality: Taka‐cho, Hyogo, Japan
Habitat and morphology of Prasiola from the marine environment in Japan (Photos by W. A. Nelson). Top left: P. calophylla on a concrete wharf in Akkeshi Bay. Bottom left: P. delicata growing next to lichens and guano on Daikokujima. Right: Prasiola cf. meridionalis on rockfaces on Daikokujima. See Sutherland et al. in this issue for details. Cover picture from: Prasiola (Prasiolales, Trebouxiophyceae) in Japan: a survey of freshwater populations and new records of marine taxa, Sutherland J et al. See pages 110‐117. Article link here
The healing process of superficial skin wounds treated with a blue‐LED haemostatic device is studied. Four mechanical abrasions are produced on the back of 10 Sprague Dawley rats: two are treated with the blue‐LED device, while the other two are left to naturally recover. Visual observations, non‐linear microscopic imaging, as well as histology and immunofluorescence analyses are performed 8 days after the treatment, demonstrating no adverse reactions neither thermal damages in both abraded areas and surrounding tissue. A faster healing process and a better‐recovered skin morphology are observed: the treated wounds show a reduced inflammatory response and a higher collagen content.
Blue LED induced photothermal effect on superficial abrasions. 相似文献
Bacterial meningitis is a disease of pronounced clinical significance, especially in the developing world. Immediate treatment with antibiotics is essential, and no single test can provide a conclusive diagnosis. It is well established that elevated total protein in cerebrospinal fluid (CSF) is associated with bacterial meningitis. Brillouin spectroscopy is a widely used optical technique for noninvasive determination of the elastic moduli of materials. We found that elevated protein levels in CSF alter the fluid elasticity sufficiently to be measurable by Brillouin spectroscopy, with model healthy and diseased fluids distinguishable to marked significance (P = 0.014), which increases with sample concentration by dialysis.
Typical raw output of a 2‐stage VIPA Brillouin spectrometer: inelastically scattered Brillouin peaks (arrows) and elastically scattered incident radiation (center cross). 相似文献
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