Cover Image Left: Flowering individuals of Gastrodia elata. Right: Individual flowers of Gastroidia elate. Photographed by Naoto Sugiura. Rebun Island, Hokkaido, Japan
Cover Image Left: Papaver fauriei growing only on the gravelly alpine slopes of Mt. Rishiri. Right: Cultivated poppies (Papaver sp.) found in seaside towns on Rishiri Island. Photographed by Kazuki Kosugi and Tetsuya Kondo. Rishiri Island, Hokkaido, Japan
Main flower‐visitng insects of Bidens fronds. Photographed by Bing Zhou in Jían City, Jiangxi Province, China. Cover picture from: Reproductive biological characteristics potentially contributed to invasiveness in an alien invasive plant Bidens frondosa, Yan XH et al. See pages 107–116. Article link here .
Cover Image Melocactus sergipensis N.P. Taylor & M.V. Meiado (Cactaceae) at the holotype locality with limestone substrate in an area of Caatinga, Simão Dias municipality, Sergipe, Brazil. Photo taken by Marcos V. Meiado.
Cover Image A seed predator Sibinia weevil visiting a flower of Dianthus superbus var. longicalycinus. Photographed by Takashi Miyake. Araihama Forest Park, Niigata, Japan.
Cover Image Left: Close‐up photograph of a hairy plant of Arabidopsis halleri subsp. gemmifera. Right: Larva of Phaedon brassicae feeding on a glabrous plant. Photographs by Yasuhiro Sato. Locality: Taka‐cho, Hyogo, Japan
Transformation of Ulva partita (Photos by R. Suzuki). Mitochondria in the 2‐cell phase are visualized using green fluorescent protein (GFP). Left: Chlorophyll autofluorescence (red). Middle: GFP fluorescence (green). Right: Merged image of GFP and chlorophyll fluorescence. See Suzuki et al. See pages 176–184. Cover picture from: Article link here
A carageenophyte, Eucheuma denticulatum (Solieriaceae, Rhodophyta) at a mariculture farm in Bali, Indonesia (Photo by R. Terada). Characteristic thermal and irradiance responses on the photosynthesis of Indonesian E. denticulatum and Kappaphycus alvarezii are reported by Borlongan et al. in this issue. Cover picture from: Article link here
Habitat and morphology of Prasiola from the marine environment in Japan (Photos by W. A. Nelson). Top left: P. calophylla on a concrete wharf in Akkeshi Bay. Bottom left: P. delicata growing next to lichens and guano on Daikokujima. Right: Prasiola cf. meridionalis on rockfaces on Daikokujima. See Sutherland et al. in this issue for details. Cover picture from: Prasiola (Prasiolales, Trebouxiophyceae) in Japan: a survey of freshwater populations and new records of marine taxa, Sutherland J et al. See pages 110‐117. Article link here
Mechanisms of renal autoregulation generate oscillations in arterial blood flow at several characteristic frequencies. Full‐field laser speckle flowmetry provides a real‐time imaging of superficial blood microcirculation. The possibility to detect changes in oscillatory dynamics is an important issue in biomedical applications. In this paper we show how laser power density affects quality of the recorded signal and improves detectability of temporal changes in microvascular perfusion.
Rather than simply acting as a photographic camera capturing two‐dimensional (x, y) intensity images or a spectrometer acquiring spectra (λ), a hyperspectral imager measures entire three‐dimensional (x, y, λ) datacubes for multivariate analysis, providing structural, molecular, and functional information about biological cells or tissue with unprecedented detail. Such data also gives clinical insights for disease diagnosis and treatment. We summarize the principles underpinning this technology, highlight its practical implementation, and discuss its recent applications at microscopic to macroscopic scales.
Datacube acquisition strategies in hyperspectral imaging x, y, spatial coordinates; λ, wavelength. 相似文献
Monostroma angicava (left) and Protomonostroma undulatum (right) collected from the same boulder at Muroran, Hokkaido, Japan with a one‐yen coin in between. Thallus of M. angicava exhibits darker green color than P. undulatum because the chlorophyll contents per area is greater in the former than the latter. See Saco et al. in this issue. The link to the article here
The paper presents problems and solutions related to hyperspectral image pre‐processing. New methods of preliminary image analysis are proposed. The paper shows problems occurring in Matlab when trying to analyse this type of images. Moreover, new methods are discussed which provide the source code in Matlab that can be used in practice without any licensing restrictions.
The proposed application and sample result of hyperspectral image analysis. 相似文献
Polarimetric measurements in multiphoton microscopy can reveal information about the local molecular order of a sample. However, the presence of a dichroic through which the excitation beam propagates will generally scramble its polarization. We propose a simple scheme whereby a second properly‐oriented compensation dichroic is used to negate any alteration regardless of the wavelength and the initial polarization. We demonstrate how this robust and rapid approach simplifies polarimetric measurements in second‐harmonic generation, two‐photon excited fluorescence and coherent anti‐Stokes Raman scattering.
Illustration of the polarization maintaining strategy with the compensating dichroic oriented such that its s‐ and p‐axes are interchanged with these of the primary dichroic. 相似文献
Cover Image A large population (left) and various life history stages (right), e.g. seedling, one-leaf, three-leaved and flowering, of Trillium camschatcense. (cf. Ohara & Kawano, 2005, Plant Species Biology 20:75–82, DOI: 10.1111/j.1442-1984.2005.00126.x ) Photographed by Masashi Ohara, Tokachi, Hokkaido, Japan.
Bacterial meningitis is a disease of pronounced clinical significance, especially in the developing world. Immediate treatment with antibiotics is essential, and no single test can provide a conclusive diagnosis. It is well established that elevated total protein in cerebrospinal fluid (CSF) is associated with bacterial meningitis. Brillouin spectroscopy is a widely used optical technique for noninvasive determination of the elastic moduli of materials. We found that elevated protein levels in CSF alter the fluid elasticity sufficiently to be measurable by Brillouin spectroscopy, with model healthy and diseased fluids distinguishable to marked significance (P = 0.014), which increases with sample concentration by dialysis.
Typical raw output of a 2‐stage VIPA Brillouin spectrometer: inelastically scattered Brillouin peaks (arrows) and elastically scattered incident radiation (center cross). 相似文献
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