共查询到20条相似文献,搜索用时 625 毫秒
1.
A sunflower cDNA microarray containing about 800 clones covering major metabolic and signal transduction pathways was used to study gene expression profiles in leaves and embryos of drought-tolerant and -sensitive genotypes subjected to water-deficit stress under field conditions. Using two-step ANOVA normalization and analysis models, we identified 409 differentially expressed genes among genotypes, water treatment and organs. The majority of the cDNA clones differentially expressed under water stress was found to display opposite gene expression profiles in drought-tolerant genotype compared to drought-sensitive genotype. These dissimilarities suggest that the difference between tolerant and non-tolerant plants seems to be associated with changes in qualitative but not quantitative mRNA expression. Comparing leaves and embryos, 82 cDNA clones showing organ-specific variation in gene expression levels were identified in response to water stress across genotypes. Genes related to amino acids and carbohydrates metabolisms, and signal transduction were induced in embryos and repressed in leaves; suggesting that vegetative and reproductive organs respond differentially to water stress. Adaptive mechanisms controlling water deficit tolerance are proposed and discussed. 相似文献
2.
3.
Functional genomics of maize submergence tolerance and cloning of the related gene Sicyp51 总被引:3,自引:0,他引:3
TANG Wanhu ZHANG Zuxin ZOU Xiling & ZHENG Yonglian . National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan China . College of Life Science Yangtze University Jingzhou China 《中国科学:生命科学英文版》2005,48(4):337-345
In the middle and lower Yangtze River area, the major corn-growing region of South China, seasonal rainfall greatly affects maize plantation. Maize seed-lings meet with excessive precipitation and low tem-perature in spring, and when they grow up to begin flowering, they usually encounter Mei-yu storm ac-companied by hot days. At the same time, bad irriga-tion system and a higher level of underground water cause waterlogging, which further leads to yield losses. In order to reveal the molecu… 相似文献
4.
In this study, SSH (Suppression Subtractive Hybridization) and cDNA microarray were used to identify genes associated with waterlogging response of maize roots. Mo17 and Hz32 are two maize inbred lines with differential tolerance to hypoxia. Seedlings of the inbred lines with two leaves were submerged in hypoxia buffer. SSH libraries were constructed with cDNA samples from roots. Both forward and reverse subtractions were performed for each inbred line, and 105 positive clones induced by hypoxia were selected by differential screening. The treated and control message RNA were hybridized with the cDNA microarray of Mo17, sequentially, 57 of 3-fold differentially expressed clones were obtained. A total of 162 positive clones were all sequenced. Bioinformatics analysis showed these positive clones represent 85 TUGs, including genes involved in several biochemistry pathways, such as glycolysis, protection, signal transduction, cell construction and energy metabolism and 41 EST with unknown function. Comparison between Mo17 and Hz32 indicates that genes related to hypoxia tolerance have different expression patterns in submerged roots. Several positive clones' expression patterns were revealed by Northern or RT-PCR, and a new gene (Sicyp51), which may contribute to hypoxia tolerance, was identified. 相似文献
5.
6.
对老龄组大鼠 (30月龄 )和年轻对照组大鼠 (3月龄 )的腓肠肌超微结构进行观察 ,可以看到前者肌肉肌纤维萎缩伴有线粒体空泡变性。并进行总RNA抽提、mRNA纯化、探针制备 ,应用基因芯片筛选老龄化相关基因 ,两组大鼠骨骼肌重复出现的差异表达基因 12 7个 ,下调基因涉及能量代谢、信号转导 ,上调基因涉及蛋白质分解、细胞凋亡 相似文献
7.
Expression profile analysis of the low-oxygen response in Arabidopsis root cultures 总被引:19,自引:0,他引:19
下载免费PDF全文
![点击此处可从《The Plant cell》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Klok EJ Wilson IW Wilson D Chapman SC Ewing RM Somerville SC Peacock WJ Dolferus R Dennis ES 《The Plant cell》2002,14(10):2481-2494
8.
9.
We are using DNA microarray-based gene expression profiling to classify temporal patterns of gene expression during the development
of maize embryos, to understand mRNA-level control of embryogenesis and to dissect metabolic pathways and their interactions
in the maize embryo. Genes involved in carbohydrate, fatty acid, and amino acid metabolism, the tricarboxylic acid (TCA) cycle,
glycolysis, the pentose phosphate pathway, embryogenesis, membrane transport, signal transduction, cofactor biosynthesis,
photosynthesis, oxidative phosphorylation and electron transfer, as well as 600 random complementary DNA (cDNA) clones from
maize embryos, were arrayed on glass slides. DNA arrays were hybridized with fluorescent dye-labeled cDNA probes synthesized
from kernel and embryo poly(A)+RNA from different stages of maize seed development. Several characteristic developmental patterns of expression were identified
and correlated with gene function. Patterns of coordinated gene expression in the TCA cycle and glycolysis were analyzed in
detail. The steady state level of poly(A)+ RNA for many genes varies dramatically during maize embryo development. Expression patterns of genes coding for enzymes of
fatty acid biosynthesis and glycolysis are coordinately regulated during development. Genes of unknown function may by assigned
a hypothetical role based on their patterns of expression resembling well characterized genes. Electronic supplementary material
to this paper can be obtained by using the Springer LINK server located at http://dx.doi.org/10.1007/s10142-002-0046-6.
Electronic Publication 相似文献
10.
11.
12.
Potokina E Sreenivasulu N Altschmied L Michalek W Graner A 《Functional & integrative genomics》2002,2(1-2):28-39
A barley cDNA macroarray comprising 1,440 unique genes was used to analyze the spatial and temporal patterns of gene expression
in embryo, scutellum and endosperm tissue during different stages of germination. Among the set of expressed genes, 69 displayed
the highest mRNA level in endosperm tissue, 58 were up-regulated in both embryo and scutellum, 11 were specifically expressed
in the embryo and 16 in scutellum tissue. Based on Blast X analyses, 70% of the differentially expressed genes could be assigned
a putative function. One set of genes, expressed in both embryo and scutellum tissue, included functions in cell division,
protein translation, nucleotide metabolism, carbohydrate metabolism and some transporters. The other set of genes expressed
in endosperm encodes several metabolic pathways including carbohydrate and amino acid metabolism as well as protease inhibitors
and storage proteins. As shown for a storage protein and a trypsin inhibitor, the endosperm of the germinating barley grain
contains a considerable amount of residual mRNA which was produced during seed development and which is degraded during early
stages of germination. Based on similar expression patterns in the endosperm tissue, we identified 29 genes which may undergo
the same degradation process.
Electronic Publication 相似文献
13.
14.
15.
16.
17.
18.
20.
cDNA微阵列技术研究干旱胁迫下柽柳基因的表达 总被引:3,自引:0,他引:3
运用cDNA微阵列技术研究干旱胁迫下柽柳(Tamarix androssowii)基因的表达。分别将Cy5和Cy3两种荧光染料标记在干旱处理和对照的柽柳cDNA上,并与载有柽柳基因的微阵列进行杂交,通过计算机对芯片进行扫描和分析研究干旱胁迫下基因的表达。共获得了47个下调表达和62个上调表达的基因。Blastx分析表明这些基因按功能可以分为脱水保护、信号传导与调控、活性氧清除、光合作用、代谢、核糖体蛋白、蛋白质的分解与再生等几大类别。同时,发现了一些与干旱胁迫相关的功能未知基因和新基因。从而揭示了柽柳具有活性氧消除、代谢调节、脱水保护、蛋白质降解与再生等抗旱途径,并阐述了干旱胁迫前后柽柳基因的差异表达。 相似文献