建成区扩张下的西安市热环境空间分异性

深入研究建成区扩张下的城市热环境的空间分异性,对于更好地认识城市热岛效应,改善城市生态环境具有重大意义。以西安市建成区为例,基于2005、2010及2016年3期Landsat遥感影像,采用线性光谱混合分析法提取建成区范围,利用热红外遥感影像反演地表温度来研究城市热环境的时空演变;在此基础上,基于城市热岛足迹与多维直方图的信息容量分析方法探究建成区扩张下城市热岛效应的影响范围变化及其空间分异性特征。结果表明:2005—2016年西安市建成区扩张强度剧烈,不透水性表面显著增加;城市建设加剧了西安市城市热岛强度和级别,致使高温区域增多并向外蔓延,逐渐呈现片状分布格局;西安市城市热岛足迹的空间分异性显著,从2005年至2016年城市热岛效应的影响足迹范围逐渐变小;信息容量能够反映城市扩张对城市热环境空间分异性的影响,研究期间西安市建成区内城市热岛空间分异性随着热岛强度增加变得更加复杂。     

快速城市化区域城市热岛及其环境效应研究

以广州为中心的珠江三角洲区域城市化十分迅速,城市热岛对城市生态环境影响极大。城市热岛及其环境效应的研究有利于城市合理规划和可持续发展。利用中尺度模式MM5模拟广州地区的城市热岛效应,模式采用四重嵌套网格,最高水平分辨率为1km。研究通过数值模拟结果与自动气象站观测的气温和ETM+热红外波段反演的地温的对比,表明该方案能准确模拟地表的温度状况和城市热岛的环境效应。研究还表明城市干岛与热岛相伴而生,02时和11时广州热岛强度较小,相对湿度较高,20时热岛强度达到最大,城区相对湿度则最低;因城市热岛的存在,改变了原盛行风向,减弱了风速,且湿地、绿地、河流廊道等景观分割了城市热岛和干岛,增加了其破碎度,调节了城市气候。     

城市水体对热岛的缓冲性能沿河岸距离的变化规律

城市水体包括城区内的自然水体和人工水体两大类。作为城市生态系统的重要组成部分,水体在缓解城市热岛效应(Urban Heat Island, UHI)上具有重要作用。研究城市热岛效应的现状,探讨水体对城市热岛的缓冲效应,为改善城市热岛效应和生态环境,并对城市进行合理的改造和规划提供理论依据。以长沙市中心城区为研究区域,以南北向贯穿长沙市的湘江河道作为主要研究对象,基于长沙市2016年7月Landsat 8 TIRS遥感影像采用大气校正法反演地表温度(Land Surface Temperature, LST),利用监督分类法获取其同步的城市化进程和土地利用类型,分析市区内地表温度及热岛效应的空间分布特征。同时,通过在湘江两侧建立多个尺度的缓冲区,并将其与地表温度分布及土地利用类型叠置,分析湘江为主的水体对长沙市热环境及各缓冲区的缓冲效应,结果表明:(1)长沙市城市建设格局与热岛效应空间分布基本相似,建筑用地热岛效应更高,极端地表温度达到53.8℃;水体、绿地和裸地的热岛效应相对较低,最低地表温度为16.0℃;(2)湘江对长沙市热岛效应具有缓冲作用,对长沙市热岛效应的平均缓冲距离为400 m;(3)湘江对热岛效应的缓冲能力与水体周边土地利用类型有关,对河东区的缓冲作用小于河西区,对沿江休闲区的缓冲效应大于沿江住宅区。综上所述,长沙市热岛效应强度与用地类型相关,建筑用地热岛效应严重;水体对热岛的缓冲效应显著,但不同缓冲区内缓冲距离存在差异,因此对城市生态格局进行小规模改造可增强水体缓冲作用,缓解城市热岛效应。     

城市热岛的生态环境效应

城市热岛效应(Urbanheatislandeffect,简称UHI)是一种由于城市建筑及人类活动导致热量在城区空间范围内聚集的现象,是城市气候最显著的特征之一。热岛引起地表温度的提高,必将强烈地影响着城市生态系统的物流、能流,改变城市生态系统结构和功能,产生一系列生态环境效应,影响着城市气候、城市水文、城市土壤理化性质、城市大气环境、城市生物习性、城市物质循环、城市能量代谢以及城市居民健康等。提高能量利用效率、优化城市格局、建设绿色屋顶、采用高反射率地表材料以及增加城市绿地均可有效地控制城市热岛效应。利用遥感手段和数值模型技术,开展多尺度的城市热岛生态环境效应研究,为改善城市生态环境、实现城市可持续发展提供理论依据。     

基于GEE的长江经济带城市群热岛特征及影响因素

遥感技术能够客观、定量地评价地表城市热岛强度的时空变化，分析城市热岛的影响因素，为城市生态建设提供有效的科学参考。本研究基于GEE(Google Earth Engine)平台，以长时间序列的MODIS卫星资料为基础数据源，使用城乡二分法估算长江经济带2003—2019年的城市地表热岛强度，再利用城市热场变异指数评价生态环境，并利用地理探测器进行城市热岛成因分析。结果表明：长江经济带5个城市群2003—2019年全年平均气温为22.78℃,白天为23.91℃,夜间为7.59℃,平均温度整体上均呈上升趋势，分别为0.02、0.02和0.006℃·a^-1。长江经济带5个城市群17年无热岛占比最高，弱热岛和弱冷岛占比次之，强热岛占比最少，且城市热岛全年、白天和夜间都呈现下降趋势。长江经济带整体的生态评价为优。地理探测器因子探测表明，DEM、土地覆盖类型、O₃是长三角城市群热岛效应的主控因子；蒸散发量、紫外线气溶胶吸收指数、人口密度是长江中游城市群热岛效应的主控因子；归一化植被指数、夜间灯光指数、土地覆盖类型是成渝城市群热岛效应的主控因子；归一化植被...     

基于多源遥感数据的城市热环境响应与归因分析——以深圳市为例

城市热环境是城市局部气候与环境的综合表现,它与土地利用格局密切相关,但是相应的机制研究还不充分。以深圳市为例,首先基于2018年LANDSAT 8遥感影像,采用支持向量机方法和线性光谱混合模型提取土地利用与覆盖度信息,分析了城市土地覆盖对地表温度以及热量收支状况的影响。基于2003-2018的MODIS地表温度数据,进一步研究了深圳市城市热岛现象的时空变化,从地表能量的角度分析城市热岛变化背后的形成机制。结果表明,深圳市地表温度从西北到东南逐渐降低,城市不透水面温度显著高于植被覆盖区域,城市热岛效应明显。不透水面和城市植被共同影响深圳市的地表温度与热量收支状况,不透水地表与感热具有较好的相关性,城市植被与潜热具有较好的相关性。长时间序列分析表明深圳的城市热岛现象在夏季较高而冬季较低,月均热岛强度为2.14℃;对于年际变化,深圳在2003-2018表现出显著的下降趋势。归因分析显示感热通量的影响在深圳起主导作用,这一模式在全年和季节上都较为明显。结果表明深圳市经过高速扩张阶段,目前发展方向是提高建成区的利用效率,该现象强调了热传输在加强城市热岛效应过程中对近地面湍流的干扰作用。本研究可以为缓解热岛效应与景观格局优化研究提供借鉴。     

北京、天津和石家庄城市地表覆盖组分与结构特征对地表温度的影响

城市地表覆盖组分与结构特征直接影响城市地表温度和热岛强度,已成为当前城市规划与城市生态学研究的热点之一。本文以北京、天津和石家庄为研究区域,基于Landsat-8 OLI/TIRS遥感影像提取城市地表覆盖,反演城市地表温度,利用城乡梯度分析、多尺度分析及景观格局指数等方法,分析了城市地表覆盖组分与结构特征对地表温度的影响。结果表明:北京、天津和石家庄不透水地表面积分别为1103.86、610.33和326.75 km2,但北京市不透水地表比例最低,为46.70%;城市内裸土的地表温度最高,其次为不透水地表和植被,水体最低;沿城乡梯度方向上,地表温度与不透水地表比例呈显著正相关,与植被比例呈显著负相关,植被覆盖比例每上升10%,地表温度下降0.4～0.6℃;随着空间分析尺度的增大,城市不透水地表和植被比例与地表温度的相关系数呈现先增大后稳定的变化趋势;城市不透水地表、植被的斑块大小、形状以及聚集度对地表温度具有显著影响,聚集度的影响最大。城市绿地在城市热岛调节中发挥着重要作用,建议在城市中合理规划城市不透水地表与植被绿地比例和空间布局,提升城市生态系统服务和人居环境质量。     

基于遥感技术的城市下垫面参数与热环境关系的研究——以北京市朝阳区为例

城市热岛（UHI）不仅直接关系到城市人居环境质量和居民健康状况,同时还对城市能源消耗、生态系统过程演变、生物物候以及城市经济可持续发展有着深远的影响。以北京市朝阳区2002—2017年夏季4期Landsat系列遥感影像为数据,采用大气反演法,对15年间朝阳区城市地表温度（LST）时空变化进行分析,发现15年间朝阳区城市热岛比例指数上升迅速,热岛效应逐年加剧。进一步研究表明,城市地表温度与归一化植被指数（NDVI）及归一化建筑指数（NDBI）密切相关：地表温度与NDBI正相关,NDBI指数每升高0.1,地表升温0.79~2.37°C;与NDVI指数负相关,NDVI指数每提高0.1,地表降温0.4~0.77°C。本研究可为城市规划建设与城市绿地营建提供科学具体的参考指导依据,并促进生态可持续发展与人居环境改善。     

海绵城市建设缓解热岛的效应与机理——以浙江省嘉兴市为例

海绵城市建设缓解热岛效应已成为全球气候变化的重要议题之一。以国家首批海绵城市试点嘉兴市为例,利用2013、2015、2017和2018年的Landsat 8遥感影像反演三环内的地表温度,通过空间和时间两个维度评估海绵城市建设对嘉兴城市热岛的缓解作用,利用Pearson相关性分析、灰色综合关联分析和回归分析等方法研究海绵城市建设的结构性指标与相对热岛强度在中小尺度层面的关系。结果表明:海绵城市建设对嘉兴热岛效应有较为明显的缓解作用,且缓解作用随着建设的不断完善而增强;海绵城市建设的结构性指标对相对热岛强度的影响程度从大到小依次为:有效不透水面积比例、下沉式绿地比例、绿地面积比例和透水路面比例;综合考虑城市热岛缓解的有效性及工程的经济性,不同指标的阈值存在差异:有效不透水面积比例应低于0.45,绿地面积比例应高于0.35,透水路面比例控制在0.4左右,下沉式绿地的应用比例则越大越好。在此基础上,提出有效缓解城市热岛效应的策略建议。     

我国三大城市群地区城市和农业用地地表热环境效应对比研究

以大中小城市协同发展为特征的城市群已成为我国城市化发展的主要形式，城市化和农业作为城市群地区最主要的土地利用活动，其气候效应是国际研究的热点。然而过去研究多关注大城市的热岛效应，对更为普遍的农业活动以及中小城市城市化的气候效应认识十分薄弱。基于MODIS地表温度数据，以自然林地为参照，提出了一种可逐像元估算土地利用地表热环境效应的新方法，进而对比分析了我国三大城市群地区（京津冀、长三角和珠三角）城市和农业用地地表热环境效应及其驱动因子差异。结果表明各城市群白天城市热岛效应明显，地级以上城市年平均热岛强度达3.2℃以上，但最强热岛均未发生在核心城市。夜晚热岛效应明显减弱，京津冀和长三角部分城市甚至出现冷岛效应。农业用地在白天亦表现出明显的增温效应，特别是在京津冀地区，而在夜晚除珠三角城市群外，降温效应明显，京津冀和长三角地区平均降温2.3℃和0.7℃。虽然城市用地平均增温强度大于农业用地，但农业用地因面积优势对区域温度变化起控制作用。白天城市和农业用地整体导致各城市群温度明显增加，京津冀增温最高（4.0℃），夜晚二者导致长三角和京津冀地区平均温度降低。研究还发现各城市群城市和农业用地地表热环境效应时空异质性极大，主要受植被、地表反照率、气候背景和人口密度控制。本文对制定缓解气候变化的土地利用策略具有重要的指导意义。     

Palytoxin Acts on Na+,K+-ATPase but not Nongastric H+,K+-ATPase

Palytoxin (PTX) opens a pathway for ions to pass through Na,K-ATPase. We investigate here whether PTX also acts on nongastric H,K-ATPases. The following combinations of cRNA were expressed in Xenopus laevis oocytes: Bufo marinus bladder H,K-ATPase α₂- and Na,K-ATPase β₂-subunits; Bufo Na,K-ATPase α₁- and Na,K-ATPase β₂-subunits; and Bufo Na,K-ATPase β₂-subunit alone. The response to PTX was measured after blocking endogenous Xenopus Na,K-ATPase with 10 μm ouabain. Functional expression was confirmed by measuring ⁸⁶Rb uptake. PTX (5 nm) produced a large increase of membrane conductance in oocytes expressing Bufo Na,K-ATPase, but no significant increase occurred in oocytes expressing Bufo H,K-ATPase or in those injected with Bufo β₂-subunit alone. Expression of the following combinations of cDNA was investigated in HeLa cells: rat colonic H,K-ATPase α₁-subunit and Na,K-ATPase β₁-subunit; rat Na,K-ATPase α₂-subunit and Na,K-ATPase β₂-subunit; and rat Na,K-ATPase β₁- or Na,K-ATPase β₂-subunit alone. Measurement of increases in ⁸⁶Rb uptake confirmed that both rat Na,K and H,K pumps were functional in HeLa cells expressing rat colonic HKα₁/NKβ₁ and NKα₂/NKβ₂. Whole-cell patch-clamp measurements in HeLa cells expressing rat colonic HKα₁/NKβ₁ exposed to 100 nm PTX showed no significant increase of membrane current, and there was no membrane conductance increase in HeLa cells transfected with rat NKβ₁- or rat NKβ₂-subunit alone. However, in HeLa cells expressing rat NKα₂/NKβ₂, outward current was observed after pump activation by 20 mm K⁺ and a large membrane conductance increase occurred after 100 nm PTX. We conclude that nongastric H,K-ATPases are not sensitive to PTX when expressed in these cells, whereas PTX does act on Na,K-ATPase.     

The sodium cycle: A novel type of bacterial energetics

The progress of bioenergetic studies on the role of Na⁺ in bacteria is reviewed. Experiments performed over the past decade on several bacterial species of quite different taxonomic positions show that Na⁺ can, under certain conditions, substitute for H⁺ as the coupling ion. Various primary Na⁺ pumps ( generators) are described, i.e., Na⁺-motive decarboxylases, NADH-quinone reductase, terminal oxidase, and ATPase. The formed is shown to be consumed by Na⁺ driven ATP-synthase, Na⁺ flagellar motor, numerous Na⁺, solute symporters, and the methanogenesis-linked reverse electron transfer system. InVibrio alginolyticus, it was found that , generated by NADH-quinone reductase, can be utilized to support all three types of membrane-linked work, i.e., chemical (ATP synthesis), osmotic (Na⁺, solute symports), and mechanical (rotation of the flagellum). InPropionigenum modestum, circulation of Na⁺ proved to be the only mechanism of energy coupling. In other species studied, the Na⁺ cycle seems to coexist with the H⁺ cycle. For instance, inV. alginolyticus the initial and terminal steps of the respiratory chain are Na⁺ - and H⁺-motive, respectively, whereas ATP hydrolysis is competent in the uphill transfer of Na⁺ as well as of H⁺. In the alkalo- and halotolerantBacillus FTU, there are H⁺ - and Na⁺-motive terminal oxidases. Sometimes, the Na⁺-translocating enzyme strongly differs from its H⁺-translocating homolog. So, the Na⁺-motive and H⁺-motive NADH-quinone reductases are composed of different subunits and prosthetic groups. The H⁺-motive and Na⁺-motive terminal oxidases differ in that the former is ofaa ₃-type and sensitive to micromolar cyanide whereas the latter is of another type and sensitive to millimolar cyanide. At the same time, both Na⁺ and H⁺ can be translocated by one and the sameP. modestum ATPase which is of the F₀F₁-type and sensitive to DCCD. The sodium cycle, i.e., a system composed of primary generator(s) and consumer(s), is already described in many species of marine aerobic and anaerobic eubacteria and archaebacteria belonging to the following genera:Vibrio, Bacillus, Alcaligenes, Alteromonas, Salmonella, Klebsiella, Propionigenum, Clostridium, Veilonella, Acidaminococcus, Streptococcus, Peptococcus, Exiguobacterium, Fusobacterium, Methanobacterium, Methanococcus, Methanosarcin, etc. Thus, the sodium world seems to occupy a rather extensive area in the biosphere.     

Role of the furosemide-sensitive Na+/K+ transport system in determining the steady-state Na+ and K+ content and volume of human erythrocytesin vitro andin vivo

Summary To study the physiological role of the bidirectionally operating, furosemide-sensitive Na⁺/K⁺ transport system of human erythrocytes, the effect of furosemide on red cell cation and hemoglobin content was determined in cells incubated for 24 hr with ouabain in 145mm NaCl media containing 0 to 10mm K⁺ or Rb⁺. In pure Na⁺ media, furosemide accelerated cell Na⁺ gain and retarded cellular K⁺ loss. External K⁺ (5mm) had an effect similar to furosemide and markedly reduced the action of the drug on cellular cation content. External Rb⁺ accelerated the Na⁺ gain like K⁺, but did not affect the K⁺ retention induced by furosemide. The data are interpreted to indicate that the furosemide-sensitive Na⁺/K⁺ transport system of human erythrocytes mediates an equimolar extrusion of Na⁺ and K⁺ in Na⁺ media (Na⁺/K⁺ cotransport), a 1:1 K⁺/K⁺ (K⁺/Rb⁺) and Na⁺/Na⁺ exchange progressively appearing upon increasing external K⁺ (Rb⁺) concentrations to 5mm. The effect of furosemide (or external K⁺/Rb⁺) on cation contents was associated with a prevention of the cell shrinkage seen in pure Na⁺ media, or with a cell swelling, indicating that the furosemide-sensitive Na⁺/K⁺ transport system is involved in the control of cell volume of human erythrocytes. The action of furosemide on cellular volume and cation content tended to disappear at 5mm external K⁺ or Rb⁺. Thein vivo red cell K⁺ content was negatively correlated to the rate of furosemide-sensitive K⁺ (Rb⁺) uptake, and a positive correlation was seen between mean cellular hemoglobin content and furosemide-sensitive transport activity. The transport system possibly functions as a K⁺ and waterextruding mechanism under physiological conditiosin vivo. The red cell Na⁺ content showed no correlation to the activity of the furosemide-sensitive transport system.     

Sodium ATPase and Sodium/proton Antiporter Are Not Obligatory for Sodium Homeostasis of Enterococcus hirae at Acid pH

Enterococcus hirae grows in a broad pH range from 5 to 11. An E. hirae mutant 7683 lacking the activities of two sodium pumps, Na⁺-ATPase and Na⁺/H⁺ antiporter, does not grow in high Na⁺ medium at pH above 7.5. We found that 7683 grew normally in high Na⁺ medium at pH 5.5. Although an energy-dependent sodium extrusion at pH 5.5 was missing, the intracellular levels of Na⁺ and K⁺ were normal in this mutant. The Na⁺ influx rates of 7683 and two other strains at pH 5.5 were much slower than those at pH 7.5. These results suggest that Na⁺ elimination of this bacterium at acid pH is achieved by a decrease in Na⁺ entry and a normal K⁺ uptake.     

Functional Consequences of Various Leucine Mutations in the M3/M4 Loop of the Na<Superscript>+</Superscript>,K<Superscript>+</Superscript>-ATPase α-Subunit

Leucines were mutated within the sequence L³¹¹ILGYTWLE³¹⁹ of the extracellular loop flanking the third (M3) and fourth (M4) transmembrane segments (M3/M4 loop) of the Torpedo Na⁺,K⁺-ATPase α-subunit. Replacement of Leu³¹¹ with Glu resulted in a considerable loss of Na⁺,K⁺-ATPase activity. Replacement of Leu³¹³ with Glu shifted the equilibrium of E₁P and E₂P toward E₁P and reduced the rate of the E₁P to E₂P transition. The reduction of the transition rate and stronger inhibition of Na⁺,K⁺-ATPase activity by Na⁺ at higher concentrations together suggest that there is interference of Na⁺ release on the extracellular side in the Leu³¹³ mutant. Thus, Leu³¹³ could be in the pathway of Na⁺ exit. Replacement of Leu³¹⁸ with Glu yielded an enzyme with significantly reduced apparent affinity for both vanadate and K⁺, with an equilibrium shifted toward E₂P and no alteration in the transition rate. The reduced vanadate affinity is due to the lower rate of production of vanadate-reactive [K⁺ ₂]E₂ caused by inhibition of dephosphorylation through reduction of the K⁺ affinity of E₂P. Thus, Leu³¹⁸ may be a critical position in guiding external K⁺ to its binding site.     

Intracellular calcium content of human erythrocytes: Relation to sodium transport systems

Summary To study the possible role of intracellular Ca (Ca _i ) in controlling the activities of the Na⁺–K⁺ pump, the Na⁺–K⁺ cotransport and the Na⁺/Li⁺ exchange system of human erythrocytes, a method was developed to measure the amount of Ca embodied within the red cell. For complete removal of Ca associated with the outer aspect of the membrane, it proved to be essential to wash the cells in buffers containing less than 20nm Ca. Ca was extracted by HClO₄ in Teflon^® vessels boiled in acid to avoid Ca contaminations and quantitated by flameless atomic absorption. Ca _i of fresh human erythrocytes of apparently healthy donors ranged between 0.9 and 2.8 mol/liter cells. The mean value found in females was significantly higher than in males. The interindividual different Ca contents remained constant over periods of more than one year. Sixty to 90% of Ca _i could be removed by incubation of the cells with A23187 and EGTA. The activities of the Na⁺–K⁺ pump, of Na⁺–K⁺ cotransport and Na⁺/Li⁺ exchange and the mean cellular hemoglobin content fell with rising Ca _i ; the red cell Na⁺ and K⁺ contents rose with Ca _i . Ca depletion by A23187 plus EGTA as well as chelation of intracellular Ca²⁺ by quin-2 did not significantly enhance the transport rates. It is concluded that the large scatter of the values of Ca _i of normal human erythrocytes reported in the literature mainly results from a widely differing removal of Ca associated with the outer aspect of the membrane.     

Phospholemman expression is high in the newborn rabbit heart and declines with postnatal maturation

Phospholemman (PLM) is a small sarcolemmal protein that modulates the activities of Na(+)/K(+)-ATPase and the Na(+)/Ca(2+) exchanger (NCX), thus contributing to the maintenance of intracellular Na(+) and Ca(2+) homeostasis. We characterized the expression and subcellular localization of PLM, NCX, and the Na(+)/K(+)-ATPase alpha1-subunit during perinatal development. Western blotting demonstrates that PLM (15kDa), NCX (120kDa), and Na(+)/K(+)-ATPase alpha-1 (approximately 100kDa) proteins are all more than 2-fold higher in ventricular membrane fractions from newborn rabbit hearts (1-4-day old) compared to adult hearts. Our immunocytochemistry data demonstrate that PLM, NCX, and Na(+)/K(+)-ATPase are all expressed at the sarcolemma of newborn ventricular myocytes. Taken together, our data indicate that PLM, NCX, and Na(+)/K(+)-ATPase alpha-1 proteins have similar developmental expression patterns in rabbit ventricular myocardium. Thus, PLM may have an important regulatory role in maintaining cardiac Na(+) and Ca(2+) homeostasis during perinatal maturation.