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1.
Yoshio Hayashi 《Journal of theoretical biology》1980,85(3):451-467
A mathematical model of protoplasmic streaming in a plant cell such as Nitella and Chara is studied. General rheological equations for the non-Newtonian fluid is derived theoretically, and the boundary value problem for the model is solved. The pattern of motion of cytoplasm in a living cell is obtained, and the rheological property of protoplasm is evaluated in vivo. 相似文献
2.
Marian Irwin 《The Journal of general physiology》1929,12(3):407-418
The rate of diffusion through the non-aqueous layer of the protoplasm depends largely on the partition coefficients mentioned above. Since these cannot be determined we have employed an artificial system in which chloroform is used in place of the non-aqueous layer of the protoplasm. The partition coefficients may be roughly determined by shaking up the aqueous solutions with chloroform and analyzing with the spectrophotometer (which is necessary with methylene blue because we are dealing with mixtures). This will show what dyes may be expected to pass through the protoplasm into the vacuole in case it behaves like the artificial system. From these results we may conclude that the artificial system and the living cell act almost alike toward methylene blue and azure B, which supports the notion of non-aqueous layers in the protoplasm. There is a close resemblance between Valonia and the artificial system in their behavior toward these dyes at pH 9.5. In the case of Nitella, on the other hand, with methylene blue solution at pH 9.2 the sap in the artificial system takes up relatively more azure B (absorption maximum at 650 mµ) than the vacuole of the living cell (655 mµ). But both take up azure B much more rapidly than methylene blue. A comparison cannot be made between the behavior of the artificial system and that of the living cell at pH 5.5 since in the latter case there arises a question of injury to cells before enough dye is collected in the sap for analysis. 相似文献
3.
OSTERHOUT WJ 《The Journal of general physiology》1950,34(2):279-284
Toxic solutions applied at one end of a Nitella cell 6 cm. long may produce little or no visible change in the structure of the protoplasm at the place of application but if the opposite end is covered with water its protoplasm soon disintegrates. If the middle of the cell is covered with mineral oil this region remains normal in appearance for half an hour or more. The result is due to the movement of substances in the cell. The loss of substances at the end where the toxic agent is applied results in loss at the opposite end if it is covered with water since water enters and travels along inside the cell carrying substances with it. This causes injury at the spot where the water enters. The conception developed here differs fundamentally from the usual view that the effects of injury spread gradually from the region where the toxic agent is applied to the immediately adjoining regions and thence to more remote places. The change produced by loss of substances produces an interesting pattern which deserves study. 相似文献
4.
W. J. V. Osterhout 《The Journal of general physiology》1944,28(1):17-22
The vacuolar surface of Nitella is covered with a non-aqueous film too thin to be visible as a separate membrane. The motion of the protoplasm may subject this film to a good deal of mechanical disturbance. Apparently this does not rupture the film for no dye escapes into the protoplasm as the result of such disturbance when the vacuolar sap is deeply stained with neutral red or brilliant cresyl blue. When the deeply stained central vacuole breaks up into several smaller vacuoles, leaving the outer protoplasmic surface in its normal position, there is no evidence of the escape of dye into the protoplasm through the film surrounding the vacuole. 相似文献
5.
OSTERHOUT WJ 《The Journal of general physiology》1950,33(4):275-284
If we apply water at one end of a Nitella cell, A, and place at the other end, B, a solution of a substance which does not penetrate, such as sucrose, water enters the cell at A, passes along inside the cell, and escapes at B. But if in place of sucrose we use a substance which penetrates such as ethyl alcohol the flow of water is lessened and this fact makes it possible to measure the amount of alcohol which enters. (An increase in the size of cells placed in solutions of alcohol does not necessarily indicate that the number of mols of alcohol entering is greater than the number of mols of water leaving the cell.) The permeability for water is more than 18 times as great as for ethyl alcohol. The behavior of the 2 substances was compared in the same individual cell with a driving force which at the start was the same for both substances. The number of mols entering per second per cm.2 of surface with a driving force of 1 atmosphere at 25°C. is 0.772 (10–6) for water and 0.042 (10–6) for ethyl alcohol. The experiments indicate that the non-aqueous substance at the surface of the protoplasm has a higher partition coefficient for water than for ethyl alcohol, although the protoplasmic surface is composed of materials not miscible with water. 相似文献
6.
Cyclic longitudinal fibrillar motion as a basis for steady rotational protoplasmic streaming 总被引:1,自引:0,他引:1
I G Donaldson 《Journal of theoretical biology》1972,37(1):75-91
The simple rhythmic motion of protoplasm back and forth along microfilaments sited at the active boundary of the streaming layer of Nitellais conjectured as a possible mechanism for the motive force for protoplasmic streaming. A theoretical model is set up to illustrate the process and estimate appropriate values for time scales and velocities of such oscillations to mantain streaming velocities of the order of those measured in these cells. 相似文献
7.
Marian Irwin 《The Journal of general physiology》1926,10(2):271-287
When living cells of Nitella are exposed to an acetate buffer solution until the pH value of the sap is decreased and subsequently placed in a solution of brilliant cresyl blue, the rate of penetration of dye into the vacuole is found to decrease in the majority of cases, and increase in other cases, as compared with the control cells which are transferred to the dye solution directly from tap water. This decrease in the rate is not due to the lowering of the pH value of the solution just outside the cell wall, as a result of diffusion of acetic acid from the cell when cells are removed from the buffer solution and placed in the dye solution, because the relative amount of decrease (as compared with the control) is the same whether the external solution is stirred or not. Such a decrease in the rate may be brought about without a change in the pH value of the sap if the cells are placed in the dye solution after exposure to a phosphate buffer solution in which the pH value of the sap remains normal. The rate of penetration of dye is then found to decrease. The extent of this decrease is the greater the lower the pH value of the solution. It is found that hydrochloric acid and boric acid have no effect while phosphoric acid has an inhibiting effect at pH 4.8 on stirring. Experiments with neutral salt solutions indicate that a direct effect on the cell (decreasing penetration) is due to monovalent base cations, while there is no such effect directly on the dye. It is assumed that the effect of the phosphate and acetate buffer solutions on the cell, decreasing the rate of penetration, is due (1) to the penetration of these acids into the protoplasm as undissociated molecules, which dissociate upon entrance and lower the pH value of the protoplasm or to their action on the surface of the protoplasm, (2) to the effect of base cations on the protoplasm (either at the surface or in the interior), and (3) possibly to the effect of certain anions. In this case the action of the buffer solution is not due to its hydrogen ions. In the case of living cells of Valonia under the same experimental conditions as Nitella it is found that the rate of penetration of dye decreases when the pH value of the sap increases in presence of NH3, and also when the pH value of the sap is decreased in the presence of acetic acid. Such a decrease may be brought about even when the cells are previously exposed to sea water containing HCl, in which the pH value of the sap remains normal. 相似文献
8.
Matilda Moldenhauer Brooks 《The Journal of general physiology》1922,4(3):347-349
Direct tests of the cell sap of Nitella show that the protoplasm is normally permeable to Li, Cs, and Sr, and that penetration is more rapid in an unbalanced than in a balanced solution. 相似文献
9.
Cutting a cell of Nitella sets up a series of rapid electrical responses, transmitted at a rate too rapid to be measured by means of our records. These are followed by slower responses whose speed falls off as the distance from the cut increases, as though they were caused by a mechanical disturbance whose intensity falls off as it travels. The faster responses seem to be due to the motion of sap past protoplasmic surfaces which have suffered little or no alteration (they seem to be similar to the electrical changes following a blow on the end of a soft rubber tube containing Ag-AgCl electrodes). The slower responses appear to be due to alterations in the protoplasm and are usually irreversible. 相似文献
10.
Marian Irwin 《The Journal of general physiology》1927,11(2):111-121
When living cells of Nitella are exposed to a solution of sodium acetate and are then placed in a solution of brilliant cresyl blue made up with a borate buffer mixture at pH 7.85, a decrease in the rate of penetration of dye is found, without any change in the pH value of the sap. It is assumed that this inhibiting effect is caused by the action of sodium on the protoplasm. This effect is not manifest if the dye solution is made up with phosphate buffer mixture at pH 7.85. It is assumed that this is due to the presence of a greater concentration of base cations in the phosphate buffer mixture. In the case of cells previously exposed to solutions of acetic acid the rate of penetration of dye decreases with the lowering of the pH value of the sap. This inhibiting effect is assumed to be due chiefly to the action of acetic acid on the protoplasm, provided the pH value of the external acetic acid is not so low as to involve an inhibiting effect on the protoplasm by hydrogen ions as well. It is assumed that the acetic acid either has a specific effect on the protoplasm or enters as undissociated molecules and by subsequent dissociation lowers the pH value of the protoplasm. With acetate buffer mixture the inhibiting effect is due to the action of sodium and acetic acid on the protoplasm. The inhibiting effect of acetic acid and acetate buffer mixture is manifested whether the dye solution is made up with borate or phosphate buffer mixture at pH 7.85. It is assumed that acetic acid in the vacuole serves as a reservoir so that during the experiment the inhibiting effect still persists. 相似文献
11.
Using multinucleate cells of Nitella 2 or 3 inches in length it is possible to kill one end with chloroform without producing at the other any immediate alteration which can be detected by our present methods. When a spot in external contact with sap is killed its potential difference falls approximately to zero and it is therefore possible to measure the potential difference across the protoplasm at any desired point merely by leading off from that point to the one where the protoplasm has been killed. The results indicate that the inner and outer protoplasmic surfaces differ, for when both surfaces are in contact with the same solution (cell sap) there is an electromotive force of about 15.9 millivolts, the inner surface being positive to the outer (i.e. the positive current tends to flow from the inner surface through the electrometer to the outer surface). The situation resembles that in Valonia where the corresponding value (with Valonia sap applied to the outside) has been reported as about 14.5 millivolt (the inner surface being positive to the outer). It would seem appropriate to designate this as radial polarity. 相似文献
12.
(a) Photosynthesis with protoplasm isolated from Chara or Nitella as measured by C14 fixation has been obtained at a rate 12 to 15 per cent of that of the whole cells. (b) Photosynthesis by cut cells of Chara or Nitella with the vacuolar sap removed was at a rate comparable to that of the whole cells. (c) Both the protoplasm and the cut cells reduced CO2 in the light to sucrose and hexose phosphates. Other products formed were also detected by paper chromatography. In contrast, dark controls fixed the C14 into products associated with plant respiration. (d) An important difference in the products from the extruded protoplasm was the absence of C14-labelled pentoses or sedoheptulose which were formed, however, by the whole or cut cells. This suggests that the most sensitive site affected by disruption of the cells may be the steps involved in the regeneration of the "C-2 acceptor" for CO2 fixation in photosynthesis. 相似文献
13.
A method distinguishing between the concentration effect due to the cell wall and that due to the protoplasm is described: the importance of this lies in the fact that if the protoplasm shows a concentration effect one or both ions of the salt must tend to enter its outer surface. Studies on the concentration effect of KCl with living protoplasm of Nitella show that when P.D. is plotted as ordinates and the logarithm of concentration as abscissæ the graph is not the straight line demanded in the ideal case by theory but has less slope and is somewhat concave to the axis of the abscissæ. With a variety of salts the dilute solution is positive, which indicates that the cation has a greater mobility in the protoplasm than the anion or that the partition coefficient of the cation (Ac) increases faster than that of the anion (Aa) as the concentration increases. If the result depended on the partition coefficients we should say that when Ac ÷ Aa increases with concentration the dilute solution is positive. When Ac ÷ Aa decreases as the concentration increases the dilute solution is negative. In either case the increase in concentration may be accompanied by an increase or by a decrease in the relative amount of salt taken up. Theoretically therefore there need be no relation between the sign of the dilute solution and the relative amount of salt taken up with increasing concentration. Hypothetical diagrams of the electrical conditions in the cell are given. If we define the chemical effect as the P.D. observed in leading off at two points with equivalent concentrations of different salts we may say that the chemical effect of the protoplasm is very much greater than that of the cell wall. 相似文献
14.
Treatment of Nitella with distilled water apparently removes from the cell something which is responsible for the normal irritability and the potassium effect, (i.e. the large P.D. between a spot in contact with 0.01 M KCl and one in contact with 0.01 M NaCl). Presumably this substance (called R) is partially removed from the protoplasm by the distilled water. When this has happened a pinch which forces sap out into the protoplasm can restore its normal behavior. The treatment with distilled water which removes the potassium effect from the outer protoplasmic surface does not seem to affect the inner protoplasmic surface in the same way since the latter retains the outwardly directed potential which is apparently due to the potassium in the sap. But the inner surface appears to be affected in such fashion as to prevent the increase in its permeability which is necessary for the production of an action current. The pinch restores its normal behavior, presumably by forcing R from the sap into the protoplasm. 相似文献
15.
Movements of water in cells of Nitella 总被引:2,自引:2,他引:0
OSTERHOUT WJ 《The Journal of general physiology》1949,32(4):553-557
When one end of a Nitella cell (A) is bathed in water and a solution of sucrose is placed at the other (B) we find that water enters at A, travels along inside the cell, and escapes at B. The solutes which cannot pass out through the protoplasm at B remain behind so that the osmotic pressure increases at B and diminishes at A until equilibrium is reached and the motion stops. An equation is given which enables us to predict with considerable accuracy the amount of flow required to produce equilibrium. 相似文献
16.
OSTERHOUT WJ 《The Journal of general physiology》1956,39(6):963-976
The behavior of the cell depends to a large extent on the permeability of the outer non-aqueous surface layer of the protoplasm. This layer is immiscible with water but may be quite permeable to it. It seems possible that a reversible increase or decrease in permeability may be due to a corresponding increase or decrease in the water content of the non-aqueous surface layer. Irreversible increase in permeability need not be due primarily to increase in the water content of the surface layer but may be caused chiefly by changes in the protoplasm on which the surface layer rests. It may include desiccation, precipitation, and other alterations. An artificial cell is described in which the outer protoplasmic surface layer is represented by a layer of guaiacol on one side of which is a solution of KOH + KCl representing the external medium and on the other side is a solution of CO2 representing the protoplasm. The K+ unites with guaiacol and diffuses across to the artificial protoplasm where its concentration becomes higher than in the external solution. The guaiacol molecule thus acts as a carrier molecule which transports K+ from the external medium across the protoplasmic surface. The outer part of the protoplasm may contain relatively few potassium ions so that the outwardly directed potential at the outer protoplasmic surface may be small but the inner part of the protoplasm may contain more potassium ions. This may happen when potassium enters in combination with carrier molecules which do not completely dissociate until they reach the vacuole. Injury and recovery from injury may be studied by measuring the movements of water into and out of the cell. Metabolism by producing CO2 and other acids may lower the pH and cause local shrinkage of the protoplasm which may lead to protoplasmic motion. Antagonism between Na+ and Ca++ appears to be due to the fact that in solutions of NaCl the surface layer takes up an excessive amount of water and this may be prevented by the addition of suitable amounts of CaCl2. In Nitella the outer non-aqueous surface layer may be rendered irreversibly permeable by sharply bending the cell without permanent damage to the inner non-aqueous surface layer surrounding the vacuole. The formation of contractile vacuoles may be imitated in non-living systems. An extract of the sperm of the marine worm Nereis which contains a highly surface-active substance can cause the egg to divide. It seems possible that this substance may affect the surface layer of the egg and cause it to take up water. A surface-active substance has been found in all the seminal fluids examined including those of trout, rooster, bull, and man. Duponol which is highly surface-active causes the protoplasm of Spirogyra to take up water and finally dissolve but it can be restored to the gel state by treatment with Lugol solution (KI + I). The transition from gel to sol and back again can be repeated many times in succession. The behavior of water in the surface layer of the protoplasm presents important problems which deserve careful examination. 相似文献
17.
In order to check whether or not the layer of chloroplasts densely arranged in the cortical gel of aNitella internode offers substantial resistance to osmotic water flow, a material was prepared which had the cortical gel layer freed from chloroplasts by centrifugation either longitudinal or lateral to the cell axis. The water permeability of the cell remained the same as normal even though the chloroplasts were exfoliated from the cell cortex to the extent of 50% of the total area, showing that the chloroplast layer plays hardly any significant part as a barrier to osmotic flow. Since it is known that the layer of the streaming endoplasm is also negligible as resistance against osmotic water flow (Tasawa and Kamiya, 1965), it is concluded that the major barrier components against osmotic flow in theNitella internode are the cell wall, plasmalemma and/or tonoplast. 相似文献
18.
D. R. Hoagland A. R. Davis With the Assistance of J. C. Martin. 《The Journal of general physiology》1923,5(5):629-646
1. Chemical examination of the cell sap of Nitella showed that the concentrations of all the principal inorganic elements, K, SO4, Ca, Mg, PO4, Cl, and Na, were very much higher than in the water in which the plants were growing. 2. Conductivity measurements and other considerations lead to the conclusion that all or nearly all of the inorganic elements present in the cell sap exist in ionic state. 3. The insoluble or combined elements found in the cell wall or protoplasm included Ca, Mg, S, Si, Fe, and Al. No potassium was present in insoluble form. Calcium was predominant. 4. The hydrogen ion concentration of healthy cells was found to be approximately constant, at pH 5.2. This value was not changed even when the outside solution varied from pH 5.0 to 9.0. 5. The penetration of NO3 ion into the cell sap from dilute solutions was definitely influenced by the hydrogen ion concentration of the solution. Penetration was much more rapid from a slightly acid solution than from an alkaline one. It is possible that the NO3 forms a combination with some constituent of the cell wall or of the protoplasm. 6. The exosmosis of chlorine from Nitella cells was found to be a delicate test for injury or altered permeability. 7. Dilute solutions of ammonium salts caused the reaction of the cell sap to increase its pH value. This change was accompanied by injury and exosmosis of chlorine. 8. Apparently the penetration of ions into the cell may take place from a solution of low concentration into a solution of higher concentration. 9. Various comparisons with higher plants are drawn, with reference to buffer systems, solubility of potassium, removal of nitrate from solution, etc. 相似文献
19.
W. J. V. Osterhout 《The Journal of general physiology》1946,29(3):181-192
The chloroplast of Spirogyra is a long, spirally coiled ribbon which may contract to form a short, nearly straight rod. This happens under natural conditions and it can also be produced by a variety of inorganic salts and by some organic substances. It also occurs when the chloroplast is freed by centrifugal force from the clear peripheral protoplasm which is in contact with the cellulose wall. It would therefore seem that the chloroplast may be passively stretched by the action of the clear protoplasm and hence it contracts as soon as it is set free. This contraction happens in dead as well as in living cells. It would be of much interest to know how the protoplasm brings about the coiling of the chloroplast and how the chloroplast is set free by various reagents. Presumably they must penetrate the living protoplasm to produce the effects described. In one species partial contraction without detachment from the peripheral protoplasm can be brought about by lead acetate. This is reversible. Lead nitrate does not produce this result. The attack upon the problem is greatly facilitated by the study of dead cells. Thereby we reduce the number of variables but the chloroplast continues to react to certain chemical and physical agents in much the same manner as in the living cell and the solution surrounding it can be controlled as is not possible in the living cell. We must await further investigation to learn what plant and animal cells contain gels under tension and what functions they perform. 相似文献
20.
Not only does distilled water take away the irritability of Nitella but it also changes its behavior toward potassium. In normal cells potassium is strongly negative to sodium but after sufficient exposure to distilled water this effect disappears. It can be restored by returning the cells to their normal environment or to a suitable nutrient solution. This change in the protoplasm seems to be chiefly in its outer surface. 相似文献