Context‐dependent conservation of the cavity‐nesting European Roller

To maximize the effectiveness of conservation interventions, it is crucial to have an understanding of how intraspecific variation determines the relative importance of potential limiting factors. For bird populations, limiting factors include nest‐site availability and foraging resources, with the former often addressed through the provision of artificial nestboxes. However, the effectiveness of artificial nestboxes depends on the relative importance of nest‐site vs. foraging resource limitations. Here, we investigate factors driving variation in breeding density, nestbox occupation and productivity in two contrasting study populations of the European Roller Coracias garrulus, an obligate cavity‐nesting insectivorous bird. Breeding density was more than four times higher at the French study site than at the Latvian site, and there was a positive correlation between breeding density (at the 1‐km² scale) and nest‐site availability in France, whereas there was a positive correlation between breeding density and foraging resource availability in Latvia. Similarly, the probability of a nestbox being occupied increased with predicted foraging resource availability in Latvia but not in France. We detected no positive effect of foraging resource availability on productivity at either site, with most variation in breeding success driven by temporal effects: a seasonal decline in France and strong interannual fluctuations in Latvia. Our results indicate that the factors limiting local breeding density can vary across a species' range, resulting in different conservation priorities. Nestbox provisioning is a sufficient short‐term conservation solution at our French study site, where foraging resources are typically abundant, but in Latvia the restoration of foraging habitat may be more important.     

Migration of herbaceous woodland flora into post-agricultural black alder woods planted on wet and fertile habitats in south western Poland

Because post-agricultural black alder woods have not been the subject of studies on forest recovery, the aim of the research was to investigate the process of colonization of their herb layer by woodland flora. Migration rates of 51 forest plant species in the herb layer of alder woods planted on abandoned meadows, bordering meso- and eutrophic forests, were studied. Mean migration rates calculated for typical wet alderwoods reached 1.20–1.60 m yr⁻¹, for oak-hornbeam forests 1.17–1.63 m yr⁻¹, and for alder-ash carrs 0.79–1.26 m yr⁻¹, exceeding those reported in studies conducted in less fertile, drier sites. Although migration rates for many species exceeded 2 or 3 m, there was a group of slow colonizing species, mainly ancient woodland indicators. Species migration patterns fitted either the model based on the establishment of isolated individuals, or the model of a wave front. The age of the recent forests and the presence of Urtica dioica in their herb layer are the main factors explaining the species composition in post-agricultural black alder woods.     

SIDEROPHORE‐INDEPENDENT IRON UPTAKE BY IRON‐LIMITED CELLS OF THE CYANOBACTERIUM ANABAENA FLOS‐AQUAE1

Iron acquisition by iron‐limited cyanobacteria is typically considered to be mediated mainly by siderophores, iron‐chelating molecules released by iron‐limited cyanobacteria into the environment. In this set of experiments, iron uptake by iron‐limited cells of the cyanobacterium Anabaena flos‐aquae (L.) Bory was investigated in cells resuspended in siderophore‐free medium. Removal of siderophores decreased iron‐uptake rates by ～60% compared to siderophore‐replete conditions; however, substantial rates of iron uptake remained. In the absence of siderophores, Fe(III) uptake was much more rapid from a weaker synthetic chelator [N‐(2‐hydroxyethyl)ethylenediamine‐N,N′,N′‐triacetic acid (HEDTA); log K_cond = 28.64 for Fe(III)HEDTA(OH)^?] than from a very strong chelator [N,N′‐bis(2‐hydroxybenzyl)‐ethylenediamine‐N,N′‐diacetic acid (HBED); log K_cond = 31.40 for Fe(III)HBED^?], and increasing chelator:Fe(III) ratios decreased the Fe(III)‐uptake rate; these results were evident in both short‐term (4 h; absence of siderophores) and long‐term (116 h; presence of siderophores) experiments. However, free (nonchelated) Fe(III) provided the most rapid iron uptake in siderophore‐free conditions. The results of the short‐term experiments are consistent with an Fe(III)‐binding/uptake mechanism associated with the cyanobacterial outer membrane that operates independently of extracellular siderophores. Iron uptake was inhibited by temperature‐shock treatments of the cells and by metabolically compromising the cells with diphenyleneiodonium; this finding indicates that the process is dependent on active metabolism to operate and is not simply a passive Fe(III)‐binding mechanism. Overall, these results point to an important, siderophore‐independent iron‐acquisition mechanism by iron‐limited cyanobacterial cells.     

Restoration of a species‐rich flood meadow by topsoil removal and diaspore transfer with plant material

Abstract. In previous studies, limited dispersal was revealed to be the main obstacle to restoration of species‐rich flood‐meadows along the northern Upper Rhine in Germany. To overcome dispersal limitation we transferred freshly mown plant material from species‐rich sources to a restoration site on a former arable field. Before plant material application, topsoil was removed to accelerate nutrient impoverishment and create favourable conditions for seedling recruitment. Topsoil removal led to a drastic reduction in organic matter and essential mineral nutrients to the level of target communities (P) or even below (N, K). At a removal depth of 30 cm content of the soil seed bank that comprised exclusively of annual arable weeds, ruderals and some common grassland species, declined by 60 ‐ 80%, while at a removal depth of 50 cm the seed bank was almost completely eliminated. With few exceptions, all species recorded in source plant material were found established at the restoration site. However, the overall correlation between seed content in plant material and establishment success was not very high. Vegetation development at the restoration site was characterized by a rapid decline in arable weeds and ruderals, while resident grassland species and species transferred with plant material increased rapidly from the third year onwards. After four years as many as 102 species were established that could be exclusively attributed to plant material transfer, among them many rare and highly endangered plants. Establishment of species from plant material was most successful in regularly flooded plots, due to the suppression of competitors as well as the creation of favourable moisture conditions for seedling emergence. Diaspore transfer with plant material proved to be an extremely successful method in restoring species‐rich grassland. However, high quality of plant material and suitable site conditions with low competition in early stages of succession seem to be essential prerequisites.     

Factors limiting rosette recruitment in scarlet gilia, Ipomopsis aggregata: seed and disturbance limitation

We performed a factorial combination of seed additions, surface soil disturbance, and protection from potential seed predation in experimental plots to elucidate the relative importance of each factor in the recruitment of scarlet gilia seedlings. We detected a significant interaction between seed addition and surface soil disturbance on seedling recruitment over 2 years of establishment. Plots that received both supplemental seed addition and disturbance established 10.5 times as many rosettes as control plots. We did not detect main or interactive effects of caging on seedling establishment. We explored the importance of density-dependent recruitment by investigating the number of rosettes per experimental plot through time. In addition, we used planned contrasts to compare the absolute and percent changes in control and treatment plot rosette densities between the study years. We found significant downward concave curvature to the seedling recruitment curve, suggesting negative density-dependent recruitment across the experimental range of rosette densities. We found a significant difference between the control plots and the seed addition-disturbance plots in terms of absolute changes in plot density from 1997 to 1998. Greater net mortality occurred in plots that received both seed addition and disturbance. However, we found no differences among treatments in the percent change in rosette density across the study years. Our study demonstrates that seedling recruitment in natural plant populations may be limited by the interaction of seed and microsite availability, and that seedling recruitment in scarlet gilia may be negatively density dependent. Our results suggest that the fecundity effects of particular plant-animal interactions (e.g., pollination, herbivory) may affect scarlet gilia population dynamics, particularly under conditions of high soil disturbance. Received: 11 June 1999 / Accepted: 24 November 1999     

Effectiveness of repeated autumn and spring fires for understorey restoration in weed‐invaded temperate eucalypt woodlands

Question. Can strategic burning, targeting differing ecological characteristics of native and exotic species, facilitate restoration of native understorey in weed‐invaded temperate grassy eucalypt woodlands? Location. Gippsland Plains, eastern Victoria, Australia. Methods. In a replicated, 5‐year experimental trial, the effects of repeated spring or autumn burning were evaluated for native and exotic plants in a representative, degraded Eucalyptus tereticornis grassy woodland. Treatments aimed to reduce seed banks and modify establishment conditions of exotic annual grasses, and to exhaust vegetative reserves of exotic perennial grasses. Treatments were applied to three grassland patch types, dominated by the native grass Austrodanthonia caespitosa, ubiquitous exotic annuals, or the common exotic perennial grass Paspalum dilatatum. Results. The dominant native grass Austrodanthonia caespitosa and native forbs were resilient to repeated fires, and target exotic annuals and perennials were suppressed differentially by autumn and spring fires. Exotic annuals were also suppressed by drought, reducing the overall treatment effects but indicating important opportunities for restoration. The initially sparse exotic geophyte Romulea rosea increased in cover with fire and the impact of this species on native forbs requires further investigation. There was minimal increase in diversity of subsidiary natives with fire, probably owing to lack of propagules. Conclusions. While fire is often considered to increase ecosystem invasibility, our study showed that strategic use of fire, informed by the relative responses of available native and exotic taxa, is potentially an effective step towards restoration of weed‐invaded temperate eucalypt woodlands.     

High‐frequency fire alters C : N : P stoichiometry in forest litter

Fire is a major driver of ecosystem change and can disproportionately affect the cycling of different nutrients. Thus, a stoichiometric approach to investigate the relationships between nutrient availability and microbial resource use during decomposition is likely to provide insight into the effects of fire on ecosystem functioning. We conducted a field litter bag experiment to investigate the long‐term impact of repeated fire on the stoichiometry of leaf litter C, N and P pools, and nutrient‐acquiring enzyme activities during decomposition in a wet sclerophyll eucalypt forest in Queensland, Australia. Fire frequency treatments have been maintained since 1972, including burning every 2 years (2yrB), burning every 4 years (4yrB) and no burning (NB). C : N ratios in freshly fallen litter were 29–42% higher and C : P ratios were 6–25% lower for 2yrB than NB during decomposition, with correspondingly lower 2yrB N : P ratios (27–32) than for NB (34–49). Trends in litter soluble and microbial N : P ratios were similar to the overall litter N : P ratios across fire treatments. Consistent with these, the ratio of activities for N‐acquiring to P‐acquiring enzymes in litter was higher for 2yrB than NB, whereas 4yrB was generally intermediate between 2yrB and NB. Decomposition rates of freshly fallen litter were significantly lower for 2yrB (72 ± 2% mass remaining at the end of experiment) than for 4yrB (59 ± 3%) and NB (62 ± 3%), a difference that may be related to effects of N limitation, lower moisture content, and/or litter C quality. Results for older mixed‐age litter were similar to those for freshly fallen litter although treatment differences were less pronounced. Overall, these findings show that frequent fire (2yrB) decoupled N and P cycling, as manifested in litter C : N : P stoichiometry and in microbial biomass N : P ratio and enzymatic activities. Furthermore, these data indicate that fire induced a transient shift to N‐limited ecosystem conditions during the postfire recovery phase.     

Regeneration processes of biological soil crusts,macro-cryptogams and vascular plant species after fine-scale disturbance in a temperate region: Recolonization or successional replacement?

Open base-rich sandy habitats are often characterised by an extensive cover of biological soil crusts (BSCs). We studied the impact of mechanical disturbances on cyanobacteria/algae-dominated BSCs, macro-cryptogams (bryophytes, lichens), phanerogams and their regeneration processes in a 3-year field experiment in a temperate region. We compared controls with two fine-scale disturbance regimes, each based on 50 plots (20 cm×20 cm): 1. BSCs were raked (weak disturbance), 2. BSCs were completely removed (strong disturbance). We used a Sileno conicae-Cerastion semidecandri habitat as a model area for the field study and ask if the crusts are able to regenerate or will be replaced by macro-cryptogams and/or phanerogams.     

Breeding systems in Clivia (Amaryllidaceae): late‐acting self‐incompatibility and its functional consequences

Late‐acting (ovarian) self‐incompatibility, characterized by minimal or zero seed production following self‐pollen tube growth to the ovules, is expected to show phylogenetic clustering, but can otherwise be difficult to distinguish from early‐acting inbreeding depression. In Amaryllidaceae, late‐acting self‐incompatibility has been proposed for Narcissus (Narcisseae) and Cyrtanthus (Cyrtantheae). Here, we investigate whether it occurs in the horticulturally important genus Clivia (Haemantheae) and test whether species in this genus experience ovule discounting in wild populations. Seed‐set results following controlled hand pollinations revealed that Clivia miniata and C. gardenii are largely self‐sterile. Self‐ and cross‐pollinated flowers of both species had similar proportions of pollen tubes entering the ovary, and those of C. gardenii also did not differ in the proportions of pollen tubes that penetrated ovules, thus ruling out classical gametophytic self‐incompatibility acting in the style, but not early inbreeding depression. Flowers that received equal mixtures of self‐ and cross‐pollen set fewer seeds than those that received cross‐pollen only, but it was unclear whether this effect was a result of ovule discounting or interactions on the stigma. The prevention of self‐pollination by the emasculation of either single flowers or whole inflorescences in wild populations did not affect seed set, suggesting that ovule discounting is not a major natural limitation on seed production. Flowers typically produce one to three large fleshy seeds from approximately 16 available ovules, even when supplementally hand pollinated, suggesting that fecundity is mostly resource limited. The results of this study suggest that Clivia spp. are largely self‐sterile as a result of either a late‐acting self‐incompatibility system or severe early inbreeding depression, but ovule discounting caused by self‐pollination is not a major constraint on fecundity. © 2014 The Linnean Society of London, Botanical Journal of the Linnean Society, 2014, 175 , 155–168.     

Seedling establishment in an epiphytic orchid: an experimental study of seed limitation

Will increased fruit and seed production in a severely pollination-limited orchid stimulate population growth? We tested whether safe sites for germination and seedling establishment are limiting for the twig epiphyte, Tolumnia variegata, by manipulating fruit set and monitoring subsequent seedling establishment for two seasons (1991–1992, 1992–1993). In the Cambalache Forest Reserve of Puerto Rico, we established 36 plots along a transect. Each plot consisted of nine trees. A center tree was designated as the site for attaching Tolumnia and manipulating fruit set. The other eight potential host trees were 1–3 and 3–5 m from the center tree in each of the cardinal directions. A 1-m length of stem 1 m from the ground was monitored for recruits on each of the nine trees of 24 fruit-enhanced plots and 12 controls (23 and 13, respectively for the 1992–1993 season). Fruit enhancement plots were divided among two treatments: one-fruit and five-fruit additions for the 1st year and one to five and more than five fruits for the 2nd year. Availability of suitable host species was not limiting. T. variegata showed little specificity for host tree species, good host trees and shrubs were common, and there was no evidence that the orchid had a preference for small branches, despite possessing the entire suite of characteristics thought to respresent “obligate” twig epiphytes. Fruit enhancement increased seed rain and seedling establishment consistently in only the high-fruit treatment plots. Most recruitment occurred near fruiting plants. Over the 2-year period, mortality was 18% for adults and 85.5% for the 1991–1992 cohort of recruits. Net recruitment was positive for both the treatment (average = 1.74) and control plots (average = 0.67). Seedling establishment at our study site was not microsite-limited. If selection for increased pollinator attraction occurs, then an increase in seed output should result in population growth.     

Flood‐deposited wood creates regeneration niches for riparian vegetation on a semi‐arid South African river

Question : The formation of large woody debris (LWD) piles during floods has significant impacts on riparian succession through pioneering plants often establishing in association with wood. We assess the importance of LWD for seed regeneration of riparian plants after a century‐scale flood disturbance in a semi‐arid environment. Location : The Sabie River within Kruger National Park in the semi‐arid northeast of South Africa. Methods : Our approach was to quantify the riparian soil seed bank, to record the frequency of establishment of riparian plants in woody debris piles, and to conduct experimental out‐plantings of common riparian trees in plots with and without LWD. Results : We found the abundance and diversity of seedlings were higher in soils taken from wood piles than from open reference areas, and most seedlings were herbaceous species. Surveys indicated that numbers of seedlings recorded within woody debris were significantly greater than in open reference areas or within established vegetation. Seedling establishment in various cover‐types also varied for different riparian tree species. Experimental out‐planting of seedlings of two riparian tree species (Philenoptera violacea and Combretum erythrophyllum) revealed that, after 433 days, planted seedlings survived only in woody debris piles. Conclusion : LWD formed after a large flood creates heterogeneous patches that may influence post‐disturbance regeneration of riparian vegetation by providing a variety of environmental niches for seedlings establishment. We suspect that higher seedling survival in LWD is due to increased moisture (particularly in the dry season) and nutrients, and protection from seasonal flooding and herbivory.