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Large-scale statistical analysis of secondary xylem ESTs in pine 总被引:3,自引:0,他引:3
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Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index 总被引:4,自引:0,他引:4
TadS. Sonstegard Anthony V. Capuco Joseph White Curtis P. Van Tassell Erin E. Connor Jennifer Cho Razvan Sultana Larry Shade James E. Wray Kevin D. Wells John Quackenbush 《Mammalian genome》2002,13(7):373-379
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The cellular and molecular biology of conifer embryogenesis 总被引:4,自引:0,他引:4
Gymnosperms and angiosperms are thought to have evolved from a common ancestor c. 300 million yr ago. The manner in which gymnosperms and angiosperms form seeds has diverged and, although broad similarities are evident, the anatomy and cell and molecular biology of embryogenesis in gymnosperms, such as the coniferous trees pine, spruce and fir, differ significantly from those in the most widely studied model angiosperm Arabidopsis thaliana. Molecular analysis of signaling pathways and processes such as programmed cell death and embryo maturation indicates that many developmental pathways are conserved between angiosperms and gymnosperms. Recent genomics research reveals that almost 30% of mRNAs found in developing pine embryos are absent from other conifer expressed sequence tag (EST) collections. These data show that the conifer embryo differs markedly from other gymnosperm tissues studied to date in terms of the range of genes transcribed. Approximately 72% of conifer embryo-expressed genes are found in the Arabidopsis proteome and conifer embryos contain mRNAs of very similar sequence to key genes that regulate seed development in Arabidopsis. However, 1388 loblolly pine (Pinus taeda) embryo ESTs (11.4% of the collection) are novel and, to date, have been found in no other plant. The data imply that, in gymnosperm embryogenesis, differences in structure and development are achieved by subtle molecular interactions, control of spatial and temporal gene expression and the regulating agency of a few unique proteins. 相似文献
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Josefa Gómez‐Maldonado Isabelle Lesur Noe Fernández‐Pozo Marina Rueda‐López Dario Guerrero‐Fernández Vanessa Castro‐Rodríguez Hicham Benzekri Rafael A. Cañas María‐Angeles Guevara Andreia Rodrigues Pedro Seoane Caroline Teyssier Alexandre Morel François Ehrenmann Grégoire Le Provost Céline Lalanne Céline Noirot Christophe Klopp Isabelle Reymond Angel García‐Gutiérrez Jean‐François Trontin Marie‐Anne Lelu‐Walter Celia Miguel María Teresa Cervera Francisco R. Cantón Christophe Plomion Luc Harvengt Concepción Avila M. Gonzalo Claros Francisco M. Cánovas 《Plant biotechnology journal》2014,12(3):286-299
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Characterization of EST-SSRs in loblolly pine and spruce 总被引:3,自引:0,他引:3
Yanik Bérubé Jun Zhuang Dainis Rungis Steven Ralph Jörg Bohlmann Kermit Ritland 《Tree Genetics & Genomes》2007,3(3):251-259
In the first large study of conifer expressed sequence tag-simple sequence repeats (EST-SSRs), two large conifer EST databases
were characterized for EST-SSRs. One database was from “interior spruce” (white and Engelmann spruce in Southern British Columbia)
and Sitka spruce, while the other was from loblolly pine. We found 475 and 629 unique EST-SSRs in loblolly pine and spruce,
respectively. 3′ ESTs contained 14% more SSRs than 5′ EST reads in loblolly pine and 41% more in spruce. Conifer EST-SSRs
differed conspicuously from angiosperm EST-SSRs in several aspects. EST-SSRs were considerably less frequent in conifers (one
EST-SSR every ∼50 kb) than in angiosperms (one EST-SSR every ∼20 kb). Dinucleotide repeats were the most abundant repeat class
in conifers, while in angiosperms, trinucleotides were most common. Finally, the AT motif was the dominant motif recovered
in both conifer species, whereas AG was the most common dinucleotide repeat in angiosperms. Also, as these EST-SSRs in conifers
could be developed into useful genetic markers, our work demonstrates the value of large-scale EST sequencing projects for
in-silico approaches for marker development. 相似文献